FastQCFastQC Report
Thu 26 May 2016
SRR1161562_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161562_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33616668
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6409231.9065631370723595No Hit
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA1281850.38131381730039393No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG978400.2910460965375867Illumina RNA PCR Primer (100% over 37bp)
AGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAA930660.2768448080577171No Hit
GAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAA774220.23030836964567694No Hit
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA710340.21130589147026704No Hit
GGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAA445050.1323896824039789No Hit
TGTGCGCACATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT433530.12896281094842593Illumina RNA PCR Primer (100% over 35bp)
GGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAAA409770.121894888571348No Hit
CTTATTCTTCTAACTACCAGTCCAAAACTAATTACAGGCCTGACAATATAA403500.120029742388508No Hit
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC370180.11011799265768993Illumina RNA PCR Primer (100% over 39bp)
GGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAGGATCA357900.10646504287694426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGC98250.034.92192518
GGGCTCA232050.034.8852421
TACCTAG99250.034.43425817
GTACCTA100650.033.9999516
TAGTTGG105350.032.05611436
ATGTACC107750.031.8642714
GAACTTA194200.031.69845213
TAAGTAG107050.031.5680732
CTATAAG111450.030.70497329
AACTACC155600.030.65687412
ACCAGTC155750.030.50904816
GTACTAT113050.030.48932626
TAACTAC157350.030.37347811
GCCTGAC162300.029.37486638
TACCAGT162300.029.2500615
CTAACTA166650.028.82746110
CCTAGCT120050.028.80531319
ATAAGTA119500.028.31666631
TGAACTT218500.028.25753212
TACAGGC168550.028.16556433
Produced by FastQC (version 0.11.5)