FastQCFastQC Report
Thu 26 May 2016
SRR1161562_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161562_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33616668
Sequences flagged as poor quality0
Sequence length51
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1311290.39007137768680705No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC921020.2739771829855356RNA PCR Primer, Index 5 (100% over 42bp)
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA787610.2342915127697962No Hit
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA564220.1678393587371598No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA543670.16172631981254063No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA484790.14421119903971447No Hit
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA404650.12037183459110223No Hit
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA346380.10303817142139132No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC343430.1021606305538669RNA PCR Primer, Index 5 (100% over 44bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACAT1019800.044.9163251
CTAACCG797300.044.9130361
GACACTC1519650.044.9083061
ACACTCA1533750.044.8820152
AGGTCGC630050.044.8796162
GCTTGCG630450.044.8483771
CTAACGA597300.044.8469961
CGACATC1072250.044.8298072
TAACGAG650650.044.8145142
GACACGA414150.044.807951
ATGTGCG1012600.044.802321
ACGACCG660850.044.797871
AGCCACG257750.044.7819941
CACACGC1154600.044.7684632
CGACTCC1009550.044.7563132
CTTGCGC651700.044.7561232
TAGGTCG599750.044.727581
GATGATC1123750.044.7178232
GATGTCC348750.044.7113722
GCTTGTC594850.044.710131
Produced by FastQC (version 0.11.5)