FastQCFastQC Report
Thu 26 May 2016
SRR1161561_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161561_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33219585
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5924011.7832883824406596No Hit
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA1218300.3667414869872697No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1004170.30228252399902045Illumina RNA PCR Primer (100% over 37bp)
AGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAA885280.26649339538708866No Hit
GAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAA743330.22376257861138243No Hit
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA665940.20046608047632142No Hit
GGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAA427790.12877644317350745No Hit
TGTGCGCACATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT424360.12774391973891305Illumina RNA PCR Primer (100% over 35bp)
GGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAAAAAAAAAAA391260.11777991808145707No Hit
CTTATTCTTCTAACTACCAGTCCAAAACTAATTACAGGCCTGACAATATAA390220.11746684975143429No Hit
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC378020.11379431741847466Illumina RNA PCR Primer (100% over 39bp)
GGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGAGGATCA344310.10364668914437071No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA224150.035.201391
ACCTAGC95600.034.55030418
TACCTAG98800.033.271317
GTACCTA99550.032.85985616
GAACTTA189650.031.9612613
TAAGTAG101300.031.91549932
TAGTTGG102250.031.7289936
TAACTAC151450.030.97626111
ACCAGTC152400.030.46963516
AACTACC153150.030.44091812
ATGTACC108000.030.43671414
GTACTAT107900.030.33684226
CTATAAG110250.029.77169229
TACCAGT156000.029.75175315
GCCTGAC157900.029.22383138
CTAACTA164200.028.54550610
TGAACTT214000.028.30390212
CCTAGCT117800.027.96276719
TACAGGC166250.027.8914233
ATGAACT217400.027.8099611
Produced by FastQC (version 0.11.5)