FastQCFastQC Report
Thu 26 May 2016
SRR1161561_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161561_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33219585
Sequences flagged as poor quality0
Sequence length51
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1093950.3293087496427183No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC913550.27500343547338113RNA PCR Primer, Index 5 (100% over 42bp)
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670540.20185080578219144No Hit
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460000.13847253058700162No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA447060.13457723809614117No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA394200.11866493816825226No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC335740.10106688569408678RNA PCR Primer, Index 5 (100% over 44bp)
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA334110.10057621129222416No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACTC1420450.044.9170951
GCTTGCG578750.044.851861
ACGACAT951600.044.8489531
ACACTCA1439800.044.8424762
AGGTCGC609000.044.84032
CTAACCG736400.044.8362661
CGACATC1008950.044.8303832
ATGTGCG955900.044.819061
CTAACGA564150.044.7999731
TAACGAG633950.044.7794042
ACGACCG625200.044.7734381
CGACTCC942200.044.758192
GACACGA391300.044.7371031
CACACGC1099700.044.7344252
CGACCGC664400.044.71532
AGCCACG244050.044.7123261
GATGATC1088100.044.708772
CGTGTTC799100.044.6919941
GCTTGTC548100.044.6871451
TAGGTCG578950.044.680891
Produced by FastQC (version 0.11.5)