FastQCFastQC Report
Thu 26 May 2016
SRR1161560_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161560_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32160773
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8843122.7496602771332643No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3376121.049763325029532Illumina RNA PCR Primer (100% over 37bp)
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1374410.42735602157323765Illumina RNA PCR Primer (100% over 39bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA766770.2384177768363963No Hit
CAGTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGG726050.22575638962409267Illumina RNA PCR Primer (97% over 37bp)
TCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG705260.2192919927639799Illumina RNA PCR Primer (100% over 40bp)
TTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC699630.21754141295049095Illumina RNA PCR Primer (100% over 32bp)
AGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT659990.2052158385620893Illumina RNA PCR Primer (100% over 38bp)
TCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG642780.1998645990256515Illumina RNA PCR Primer (100% over 33bp)
GTTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT580050.18035947083734585Illumina RNA PCR Primer (100% over 31bp)
TACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT484590.15067734845801126Illumina RNA PCR Primer (100% over 35bp)
GTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC431930.13430336391479147Illumina RNA PCR Primer (97% over 39bp)
CCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC425230.13222008065539967Illumina RNA PCR Primer (100% over 41bp)
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA412100.12813746734259154No Hit
CTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG387830.12059100693879467Illumina RNA PCR Primer (100% over 34bp)
GCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC370170.11509984539239775Illumina RNA PCR Primer (100% over 41bp)
ACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG333400.10366666248973555Illumina RNA PCR Primer (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGC112150.034.80658318
GTACCTA112100.034.54144716
TACCTAG113600.034.3230717
TAAGTAG112200.034.04934332
ATGTACC117700.033.12760514
TAGTTGG117000.032.97935536
TGGTCGC171050.032.63709345
GTACTAT120350.032.41625626
CCTAGCT121950.032.08324419
ATAAGTA123300.031.11156531
TGTACTA130000.030.04463225
GTAGTTG129600.029.91191335
CTATAAG130350.029.6877529
TAGCTGT133250.029.2611821
CTAGCTG135050.029.00445420
TATGTAC135800.028.84534513
GCTGTAC136900.028.8261523
TATAAGT138450.027.65840330
AGTTGGT142200.027.2139837
AAGTAGT142350.027.09051333
Produced by FastQC (version 0.11.5)