FastQCFastQC Report
Thu 26 May 2016
SRR1161560_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161560_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32160773
Sequences flagged as poor quality0
Sequence length51
%GC28

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC3409611.060176631948492RNA PCR Primer, Index 12 (100% over 42bp)
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1967620.611807433857389No Hit
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1772930.551270953593062No Hit
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1736800.5400367708823416No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC1405000.43686760887246084RNA PCR Primer, Index 12 (100% over 44bp)
ACGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA861050.267732992611838No Hit
GTCGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT705260.2192919927639799RNA PCR Primer, Index 12 (100% over 43bp)
GTCGGACTGTAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG626810.1948989223611012RNA PCR Primer, Index 12 (100% over 37bp)
GTCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG619120.19250781067979927RNA PCR Primer, Index 12 (100% over 45bp)
GTCGGACTGTAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT569500.17707907704830353RNA PCR Primer, Index 12 (100% over 38bp)
GTCGGACTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA480940.14954242548834257RNA PCR Primer, Index 12 (100% over 40bp)
GTCGGACTGTAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT480250.1493278784064052RNA PCR Primer, Index 12 (100% over 36bp)
GTCGTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA415340.12914490581429744No Hit
CATCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA374000.1162907371660501No Hit
GTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT364460.11332439055491608RNA PCR Primer, Index 12 (100% over 46bp)
GTCGGACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA352690.1096646526499845RNA PCR Primer, Index 12 (100% over 39bp)
GTCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT344230.10703411886275246RNA PCR Primer, Index 12 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG1109650.044.8592451
ACGACAT1154700.044.8144261
ACGACCG1316100.044.8126831
TGATGCG2211200.044.7874531
TCACACG954750.044.7358971
CACACGC972650.044.7310452
GATGTCC947000.044.7259062
GTGTCGC1129200.044.705772
GTCGTTC998500.044.7005461
CGACCGC1379000.044.6953622
CGTGAAG419600.044.694083
CGACGAA1155050.044.683322
CGACTCC870250.044.6751062
TCGTGAA1214150.044.671362
GACGAAG391800.044.6662643
GATGCGC2238750.044.655862
CGACATC1211200.044.6426662
ACACGCG378600.044.6366233
GACGAAT283900.044.624673
ATGCGCG797500.044.6242073
Produced by FastQC (version 0.11.5)