FastQCFastQC Report
Thu 26 May 2016
SRR1161559_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161559_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31821460
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8165282.565966489281133No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG3418311.0742153251296451Illumina RNA PCR Primer (100% over 37bp)
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1385860.4355111299104441Illumina RNA PCR Primer (100% over 39bp)
CAGTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGG733080.23037283644433662Illumina RNA PCR Primer (97% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA719260.2260298553240486No Hit
TTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC717730.22554904771811224Illumina RNA PCR Primer (100% over 32bp)
TCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG716780.22525050704775962Illumina RNA PCR Primer (100% over 40bp)
AGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT668570.2101003536607057Illumina RNA PCR Primer (100% over 38bp)
TCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG652480.2050440174649435Illumina RNA PCR Primer (100% over 33bp)
GTTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT581290.1826723223887276Illumina RNA PCR Primer (100% over 31bp)
TACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT490290.15407526870231597Illumina RNA PCR Primer (100% over 35bp)
CCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC429700.13503465900056127Illumina RNA PCR Primer (100% over 41bp)
GTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC424790.1334916751148439Illumina RNA PCR Primer (97% over 39bp)
CTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG397670.1249691246096188Illumina RNA PCR Primer (100% over 34bp)
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA393830.12376239179471968No Hit
GCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC374130.11757160105161737Illumina RNA PCR Primer (100% over 41bp)
ACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG337950.10620191531123964Illumina RNA PCR Primer (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA101850.034.59166316
ACCTAGC102250.034.41601618
TACCTAG102300.034.24469417
TAAGTAG104450.033.04248432
TGGTCGC179700.032.84392545
TAGTTGG107650.032.2692636
ATGTACC109350.032.24111614
GTACTAT111750.031.70766326
CCTAGCT114950.030.78985219
ATAAGTA113450.030.59840431
CTATAAG122200.029.08798629
TGTACTA124650.028.96892425
GTAGTTG120900.028.90020435
TAGCTGT123700.028.64828921
CTAGCTG125500.028.41668320
TATGTAC124900.028.38967913
GCTGTAC127000.028.11648623
TATAAGT125250.027.76930430
AGTTGGT132900.026.6291637
AAGTAGT131050.026.6275133
Produced by FastQC (version 0.11.5)