FastQCFastQC Report
Thu 26 May 2016
SRR1161559_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161559_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31821460
Sequences flagged as poor quality0
Sequence length51
%GC28

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC3334021.0477269113359349RNA PCR Primer, Index 12 (100% over 42bp)
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1693550.5322037392376088No Hit
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1504390.4727595779703383No Hit
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1489280.4680112100450451No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC1363650.42853156329093633RNA PCR Primer, Index 12 (100% over 44bp)
ACGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA711160.22348440329262076No Hit
GTCGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT689040.21653311947346224RNA PCR Primer, Index 12 (100% over 43bp)
GTCGGACTGTAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG627340.19714368856740072RNA PCR Primer, Index 12 (100% over 37bp)
GTCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG607730.19098118062464764RNA PCR Primer, Index 12 (100% over 45bp)
GTCGGACTGTAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT567040.17819421233343788RNA PCR Primer, Index 12 (100% over 38bp)
GTCGGACTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA485340.15251971468311007RNA PCR Primer, Index 12 (100% over 40bp)
GTCGGACTGTAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT477060.14991769705098384RNA PCR Primer, Index 12 (100% over 36bp)
GTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT362500.11391683473982651RNA PCR Primer, Index 12 (100% over 46bp)
GTCGTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360600.11331975339912122No Hit
GTCGGACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA358540.1126723915244618RNA PCR Primer, Index 12 (100% over 39bp)
GTCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT338760.10645646051438244RNA PCR Primer, Index 12 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCG1038900.044.839441
ACGACCG1210750.044.803641
ACGACAT1084450.044.7800451
TGATGCG2130600.044.7633061
GTGTCGC1066900.044.7328722
GTCGTTC973650.044.7103921
CGACCGC1280300.044.69212
CACACGC923700.044.6687662
TCACACG902050.044.666951
GATGTCC918150.044.6422272
CGTGAAG402400.044.6397133
CGACATC1152300.044.6358682
GATGCGC2164950.044.6255722
TGTCGCA327850.044.6119773
TGTCGCG425950.044.5911373
ACACGCG374150.044.5822033
CGACGAA1073800.044.569072
CGACTCC814600.044.565732
TCGTGAA1146500.044.5554922
GACTCCG335650.044.5540243
Produced by FastQC (version 0.11.5)