FastQCFastQC Report
Thu 26 May 2016
SRR1161558_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161558_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34143410
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4454331.3045943565683684No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1807370.5293466586963634Illumina RNA PCR Primer (100% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA1294580.37915955084744024No Hit
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1025940.3004796533210948Illumina RNA PCR Primer (100% over 39bp)
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA467150.13681996027930426No Hit
AGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT375670.11002708868270628Illumina RNA PCR Primer (100% over 38bp)
ATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATC360670.10563385438068429No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA166050.037.20660816
ACCTAGC168950.036.9275718
TACCTAG170150.036.4688517
TAAGTAG173200.035.31992332
GTACTAT177050.035.0726426
ATGTACC179150.034.5993314
TAGTTGG177250.034.38596336
CCTAGCT189250.033.00213619
CTATAAG189400.032.91636329
ATAAGTA186450.032.8580531
TGTACTA197850.031.49920525
GTAGTTG198350.031.000335
TATGTAC201350.030.81857113
CTAGCTG205350.030.74336620
TAGCTGT204050.030.57536921
GCTGTAC206950.030.34260623
AAGTAGT208550.029.50567833
AGTTGGT210800.029.28684637
TATAAGT210650.029.11519230
AGTAGTT213050.028.7557434
Produced by FastQC (version 0.11.5)