FastQCFastQC Report
Thu 26 May 2016
SRR1161558_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161558_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34143410
Sequences flagged as poor quality0
Sequence length51
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC1618110.4739157570963181RNA PCR Primer, Index 19 (100% over 42bp)
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC904870.2650203948580414RNA PCR Primer, Index 19 (100% over 44bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620920.1818564695207655No Hit
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA401630.1176303128480723No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG556400.044.8777851
GACACGA796150.044.8444821
CTAACGA801500.044.8343661
ACGACAT690450.044.825471
ACACGAG804400.044.8120962
AGGTCGC461750.044.8109052
GACACCG772500.044.8044171
ACGACCG792400.044.8039321
ACACCGC782700.044.7864652
CTAACCG599400.044.763461
ATGTGCG693000.044.7579541
CGACCGC835250.044.7547652
TAACGAG868300.044.7541432
GACACTC791400.044.7382621
GCCACGC465800.044.7256742
TAGGTCG441150.044.7255821
CGTGTTC698650.044.7116851
CGACGAA679700.044.6961862
AACGAGC208550.044.6930733
GATGCGC1109200.044.6637882
Produced by FastQC (version 0.11.5)