FastQCFastQC Report
Thu 26 May 2016
SRR1161557_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161557_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34439319
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4210041.222451582158172No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1811720.526061505455436Illumina RNA PCR Primer (100% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA1258780.36550664663258875No Hit
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1027040.2982172789189008Illumina RNA PCR Primer (100% over 39bp)
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA463450.13457002445373556No Hit
AGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT370710.1076415012735879Illumina RNA PCR Primer (100% over 38bp)
ATTCTCCAGGCATACGCGTATAACAACTCGGATAACCATTGTTAGTTAATC351240.10198807938101215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTAG170300.036.55721717
GTACCTA170950.036.4942216
ACCTAGC172000.036.31431218
TAAGTAG171700.035.92960432
ATGTACC177750.035.0494514
GTACTAT180050.034.8486126
TAGTTGG177250.034.779236
ATAAGTA181650.033.9726931
CTATAAG187750.033.49124529
CCTAGCT191600.032.5760519
TGTACTA195950.032.079525
GTAGTTG197600.031.6187235
TATGTAC200700.030.9073913
TAGCTGT206550.030.43607521
GCTGTAC207400.030.3438423
CTAGCTG210500.030.14286220
AGTAGTT210100.029.52337634
TATAAGT210300.029.50462330
AGTTGGT212750.029.48312237
AAGTAGT212550.029.25716433
Produced by FastQC (version 0.11.5)