FastQCFastQC Report
Thu 26 May 2016
SRR1161557_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161557_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34439319
Sequences flagged as poor quality0
Sequence length51
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC1567150.4550467446815659RNA PCR Primer, Index 19 (100% over 42bp)
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC873450.25361999753827885RNA PCR Primer, Index 19 (100% over 44bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537190.1559815976616727No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA771600.044.8918951
GACACGA760950.044.8311581
ACGACCG773950.044.8108251
GCTTGCG533350.044.7862321
TAACGAG858450.044.776662
CGACCGC823850.044.7752762
ACACGAG775450.044.760962
ACGACAT660600.044.760371
GACACCG723750.044.7444571
AGGTCGC449850.044.7345962
CTAACCG579650.044.714471
CGACGAA650350.044.7140732
ATGTGCG653900.044.7096751
ACACCGC739000.044.6971742
GACACTC751150.044.6970671
GCCACGC469450.044.65482
GTCGTTC553800.044.63181
GATGCGC1106250.044.6263732
CGACTCC639150.044.6139222
CGTGTTC655200.044.600361
Produced by FastQC (version 0.11.5)