FastQCFastQC Report
Thu 26 May 2016
SRR1161556_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161556_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20307476
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5073732.4984542638385983No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1618580.7970365199495989Illumina RNA PCR Primer (100% over 37bp)
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1058020.5210002464117155Illumina RNA PCR Primer (100% over 39bp)
TCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG549800.27073773225190567Illumina RNA PCR Primer (100% over 40bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA508920.250607214801091No Hit
GTTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT455670.22438534458935222Illumina RNA PCR Primer (100% over 31bp)
TTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC455590.22434595023034867Illumina RNA PCR Primer (100% over 32bp)
TCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG435700.21455152772309077Illumina RNA PCR Primer (100% over 33bp)
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA412730.20324042239419612No Hit
AGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT385670.1899152804612449Illumina RNA PCR Primer (100% over 38bp)
CCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC351100.17289199307683534Illumina RNA PCR Primer (100% over 41bp)
TGTGCGCACATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT325320.16019716088794098Illumina RNA PCR Primer (100% over 35bp)
CTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG276030.135925311446878Illumina RNA PCR Primer (100% over 34bp)
TACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT252110.12414639810481617Illumina RNA PCR Primer (100% over 35bp)
CAGTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGG237310.11685844168915921Illumina RNA PCR Primer (97% over 37bp)
GTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC237120.11676488008652576Illumina RNA PCR Primer (97% over 39bp)
ACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG216220.10647310379684802Illumina RNA PCR Primer (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTAG70250.036.3831417
ACCTAGC70650.036.33244718
GTACCTA72250.035.4073116
TGGTCGC127450.033.97911545
TAAGTAG75500.033.79949632
TAGTTGG75350.033.74732236
ATGTACC76600.033.28515614
GTACTAT78450.032.94945526
CCTAGCT78650.032.6654419
ATAAGTA80350.031.841731
CTAGCTG81750.031.61938320
GTAGTTG82100.030.97272735
CTATAAG85200.030.36744329
TGTACTA85800.030.17929825
TATGTAC85050.029.89878813
TATAAGT85700.029.88002630
TAGCTGT88100.029.2126721
GCTGTAC89050.029.1031323
AAGTAGT88850.028.89830233
GGGCTCA80800.028.7892481
Produced by FastQC (version 0.11.5)