FastQCFastQC Report
Thu 26 May 2016
SRR1161556_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161556_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20307476
Sequences flagged as poor quality0
Sequence length51
%GC27

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC1732110.8529420396705135RNA PCR Primer, Index 12 (100% over 42bp)
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC1343190.6614263633747492RNA PCR Primer, Index 12 (100% over 44bp)
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA751050.36983916662021415No Hit
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA673760.33177929152790825No Hit
GTCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG609700.3002342585558146RNA PCR Primer, Index 12 (100% over 45bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA453590.2233610912552599No Hit
GTCGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT444400.21883566426472695RNA PCR Primer, Index 12 (100% over 43bp)
GTCGGACTGTAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG432900.21317272515796645RNA PCR Primer, Index 12 (100% over 37bp)
GTCGGACTGTAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT424710.20913972765497793RNA PCR Primer, Index 12 (100% over 36bp)
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA421810.20771168214109917No Hit
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA403360.19862635809590515No Hit
GTCGGACTGTAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT398730.19634640956857466RNA PCR Primer, Index 12 (100% over 38bp)
ACGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA383520.18885655706302448No Hit
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380080.18716259962587176No Hit
GTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT371300.18283906872523203RNA PCR Primer, Index 12 (100% over 46bp)
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA313390.1543224771015364No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA291930.1437549402988338No Hit
ATGTGCGCACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA270080.13299535599598886RNA PCR Primer, Index 12 (100% over 40bp)
GTCGGACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA265370.1306760131096548RNA PCR Primer, Index 12 (100% over 39bp)
GCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA264290.13014418926310684No Hit
GTCGGACTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA255540.12583543124709343RNA PCR Primer, Index 12 (100% over 40bp)
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226870.11171747783919578No Hit
GTCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT214270.10551286629613646RNA PCR Primer, Index 12 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGA529300.044.979431
GCTTGCG343800.044.9567221
CTAACTC755050.044.927221
ACACGAG529850.044.919972
GACACTC628350.044.9078561
CGACGAA596250.044.9053732
AGCCACG294800.044.880291
TAACGAG346800.044.8733942
CACACGC571900.044.8697242
GCTTGTC476750.044.867441
ATGTGCG996400.044.8636321
ACGACCG419550.044.852611
AGGTCGC413650.044.849162
GCCACGC293350.044.84082
GATGTCC549550.044.840072
GACACCG318800.044.8327641
AGCCATC487900.044.8264121
ACACTCA622250.044.8226852
CTAACGA349050.044.7714651
GATGCGC922350.044.764432
Produced by FastQC (version 0.11.5)