FastQCFastQC Report
Thu 26 May 2016
SRR1161555_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161555_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20117132
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260872.615119292352409No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1731570.8607439668835499Illumina RNA PCR Primer (100% over 37bp)
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC1106550.5500535563419279Illumina RNA PCR Primer (100% over 39bp)
TCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG574370.2855128653527749Illumina RNA PCR Primer (100% over 40bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA531470.264187757976634No Hit
TTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC484480.24082955761288438Illumina RNA PCR Primer (100% over 32bp)
GTTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT483240.2402131675628514Illumina RNA PCR Primer (100% over 31bp)
TCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG458820.22807426028720199Illumina RNA PCR Primer (100% over 33bp)
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA428340.2129229951863914No Hit
AGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGT405380.20150983748578077Illumina RNA PCR Primer (100% over 38bp)
CCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC369750.18379856532233324Illumina RNA PCR Primer (100% over 41bp)
TGTGCGCACATGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT336040.16704170355893674Illumina RNA PCR Primer (100% over 35bp)
CTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGG287230.14277880167013868Illumina RNA PCR Primer (100% over 34bp)
TACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGT261310.12989426126944933Illumina RNA PCR Primer (100% over 35bp)
CAGTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGG252810.1256690068942233Illumina RNA PCR Primer (97% over 37bp)
GTCCGACGATCGTCGTACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC246290.12242798824404989Illumina RNA PCR Primer (97% over 39bp)
ACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTG224920.11180520165598158Illumina RNA PCR Primer (100% over 36bp)
GCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC211050.10491058069311272Illumina RNA PCR Primer (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGC114600.036.12098345
ACCTAGC70300.035.87373718
GTACCTA69900.035.7287216
TACCTAG71200.035.07419217
TAAGTAG71500.034.99648332
ATGTACC72900.034.3884414
GTACTAT75250.033.42424826
TAGTTGG74750.033.26466836
CCTAGCT76850.033.0796519
ATAAGTA76700.032.7677631
TGTACTA80600.031.42893425
CTAGCTG82200.031.11823720
CTATAAG81900.030.95853629
GTAGTTG82800.030.30237635
GGGCTCA79950.030.1659281
TATGTAC83000.030.12285813
TATAAGT84350.029.87536830
TAGCTGT87700.029.1153921
GCTGTAC87450.028.94136823
AAGTAGT88000.028.5117833
Produced by FastQC (version 0.11.5)