FastQCFastQC Report
Thu 26 May 2016
SRR1161555_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161555_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20117132
Sequences flagged as poor quality0
Sequence length51
%GC27

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATC1714020.852020059320583RNA PCR Primer, Index 12 (100% over 42bp)
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTC1337640.6649257955855735RNA PCR Primer, Index 12 (100% over 44bp)
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA718050.356934576956596No Hit
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA652040.3241217485673405No Hit
GTCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG603950.3002167505785616RNA PCR Primer, Index 12 (100% over 45bp)
GTCGGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCT446090.22174632049936344RNA PCR Primer, Index 12 (100% over 43bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA434450.21596020744905386No Hit
GTCGGACTGTAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG431180.21433472723646688RNA PCR Primer, Index 12 (100% over 37bp)
GTCGGACTGTAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT417310.20744010627359805RNA PCR Primer, Index 12 (100% over 36bp)
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA409770.20369205709839752No Hit
GTCGGACTGTAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGT397500.19759277813557122RNA PCR Primer, Index 12 (100% over 38bp)
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390500.19411315688538505No Hit
GTCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGT376070.1869401662225013RNA PCR Primer, Index 12 (100% over 46bp)
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370210.18402722614734546No Hit
ACGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA368630.18324182592230345No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300270.14926083897048548No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA278160.13827020670739745No Hit
GTCGGACTGTAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA274000.13620231750728684RNA PCR Primer, Index 12 (100% over 39bp)
ATGTGCGCACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA267550.1329960950696153RNA PCR Primer, Index 12 (100% over 40bp)
GTCGGACTGTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAA254590.12655382486927064RNA PCR Primer, Index 12 (100% over 40bp)
GCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250880.12470962560667198No Hit
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA218720.10872325140581669No Hit
GTCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAAT210730.1047515122931042RNA PCR Primer, Index 12 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGCG341450.044.978931
GACACGA529900.044.9399221
CTAACTC743100.044.938951
ACACGAG529650.044.918582
AGGTCGC411850.044.9148862
ATGTGCG1004800.044.914061
ACGACCG414650.044.8834151
CGACGAA580250.044.8825452
TAACGAG353550.044.880532
ACGACAT308400.044.8725851
GCCACGC284500.044.8706322
GACACTC619450.044.840741
AGCCACG285400.044.8318141
GATGTCC551900.044.814992
GCTTGTC471150.044.8118441
CTAACGA354500.044.7984161
GTGTTCA585800.044.7959562
GACACCG314050.044.7902681
CGTGTTC587350.044.769851
AGCCATC499000.044.768341
Produced by FastQC (version 0.11.5)