FastQCFastQC Report
Thu 26 May 2016
SRR1161554_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161554_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31273378
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7875532.518285680555519No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1178170.37673256787290454Illumina RNA PCR Primer (100% over 37bp)
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC698500.223352910581006Illumina RNA PCR Primer (100% over 39bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA470310.15038669631403426No Hit
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA452670.14474611600959766No Hit
GTTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT397150.12699299704688122Illumina RNA PCR Primer (100% over 31bp)
TCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG364330.11649844797706214Illumina RNA PCR Primer (100% over 33bp)
TTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC354000.11319531903461147Illumina RNA PCR Primer (100% over 32bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA73300.032.59799216
ACCTAGC74450.032.0611218
TACCTAG76300.031.28675317
TAGTTGG78500.030.44311536
ATGTACC82950.029.05488214
GGGCTCA107800.028.6745051
CCTAGCT83600.028.57894719
GTACTAT86750.028.0858126
TGGTCGC79900.026.88961445
TGTACTA91700.026.61879225
GTAGTTG93050.026.43248635
TATGTAC93450.025.93476913
CTATAAG94550.025.86550529
ATAAGTA95250.025.65499931
CTAGCTG96150.025.36342620
TATAAGT97350.025.05522730
TAGCTGT97950.024.92030121
GCTGTAC99350.024.68235423
TAAGTAG101050.024.38439832
GAACTTA101300.023.50294313
Produced by FastQC (version 0.11.5)