FastQCFastQC Report
Thu 26 May 2016
SRR1161554_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161554_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31273378
Sequences flagged as poor quality0
Sequence length51
%GC26

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC1215320.3886116811557741RNA PCR Primer, Index 19 (100% over 42bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA879660.28128077497736254No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC854530.27324518636905804RNA PCR Primer, Index 19 (100% over 44bp)
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA784890.2509770450764865No Hit
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA652620.20868228561685917No Hit
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA613090.19604214165799425No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA554470.1772977642517543No Hit
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA514370.16447535664359636No Hit
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA499510.15972371133044855No Hit
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA445360.1424086646476118No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA417940.1336408238342529No Hit
ACGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359530.114963596193542No Hit
GTCGGACTGTAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG354150.11324328315284649RNA PCR Primer, Index 15 (97% over 36bp)
GTCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG343510.10984102836604348RNA PCR Primer, Index 19 (100% over 45bp)
GTCGGACTGTAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA327400.10468968206760396RNA PCR Primer, Index 19 (100% over 37bp)
GTCGGACTGTAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA320010.10232664984255939RNA PCR Primer, Index 19 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAG1231500.044.9075782
CTAACGA1234050.044.891511
GACACGA713550.044.8802871
CGTGTCG2103600.044.860541
ACACGAG710350.044.850782
AGCCATC1361550.044.8470651
CGTGTTC900000.044.8082581
GACACTC721050.044.794911
AGGTCGC420950.044.78892
ACGACCG941550.044.782331
GTGTCGC2092300.044.7731062
CTAACCG1006250.044.763951
ATGTGCG1005100.044.7299351
GCTTGTC765400.044.714741
GACACCG554900.044.706911
TGTCGCA533750.044.702863
GCTTGCG405950.044.7000661
TGTCGCG729750.044.6934363
CGACCGC932200.044.6801032
CACACGC694950.044.670332
Produced by FastQC (version 0.11.5)