FastQCFastQC Report
Thu 26 May 2016
SRR1161553_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161553_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31287097
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8267192.642364039079752No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG1277800.4084111734623382Illumina RNA PCR Primer (100% over 37bp)
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC734070.23462387705705007Illumina RNA PCR Primer (100% over 39bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA493790.15782544478319607No Hit
ACCAAAAATAATGAACTTAAAACAAAAATATAATTATTCATTATTTCTACA476970.15244942667579547No Hit
GTTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT434840.13898381176112312Illumina RNA PCR Primer (100% over 31bp)
TTCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTC383780.12266398509264058Illumina RNA PCR Primer (100% over 32bp)
TCTACAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG382980.12240828863093306Illumina RNA PCR Primer (100% over 33bp)
TCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCG327510.10467893521728781Illumina RNA PCR Primer (100% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA73450.032.01104716
ACCTAGC74250.031.87530918
TACCTAG75800.030.987217
TAGTTGG78650.030.29884536
GGGCTCA105400.029.6913971
TGGTCGC73150.029.6789245
ATGTACC83150.028.52447114
CCTAGCT83450.028.5229519
GTACTAT84550.028.39134226
TGTACTA92250.026.16787325
GTAGTTG93300.025.8066135
CTAGCTG94800.025.60638220
CTATAAG95250.025.438929
ATAAGTA95000.025.34274331
TATGTAC96650.024.58695813
TAGCTGT98200.024.49070721
TATAAGT99900.024.1441330
GAACTTA100600.024.00183513
GCTGTAC100850.023.98102223
TAAGTAG101150.023.84816432
Produced by FastQC (version 0.11.5)