FastQCFastQC Report
Thu 26 May 2016
SRR1161553_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161553_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31287097
Sequences flagged as poor quality0
Sequence length51
%GC26

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATC1220300.3900329902771101RNA PCR Primer, Index 19 (100% over 42bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA855390.27340024547499564No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTC853340.27274502329187017RNA PCR Primer, Index 19 (100% over 44bp)
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA765640.2447142986771831No Hit
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640870.20483523926812386No Hit
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA602390.19253623945999207No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA536390.1714412813691216No Hit
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA501710.16035683975410053No Hit
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA485690.15523651810840744No Hit
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA434910.13900618520152253No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA401620.12836601618871832No Hit
GTCGGACTGTAGAACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG356970.11409495741966728RNA PCR Primer, Index 15 (97% over 36bp)
ACGACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350160.11191834128938202No Hit
GTCGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAAAATCTCG345850.11054077660193275RNA PCR Primer, Index 19 (100% over 45bp)
GTCGGACTGTAGAATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGA331920.10608846196245053RNA PCR Primer, Index 19 (100% over 37bp)
GTCGGACTGTAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGAA320730.10251190770431658RNA PCR Primer, Index 19 (100% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAG1235650.044.94952
CTAACGA1237600.044.9060021
GACACGA725100.044.8904651
CGTGTCG2115100.044.87211
AGCCATC1358550.044.8706171
ACACGAG722150.044.8459662
GACACTC725250.044.809691
CGTGTTC887700.044.7815931
ACGACCG931900.044.7815321
GTGTCGC2110900.044.7734262
AGGTCGC420250.044.7591172
CTAACCG1014350.044.7485241
GCTTGTC776950.044.7381551
GACACCG571150.044.735051
GCCATCA1353750.044.723312
ATGTGCG998700.044.720661
GCTTGCG403050.044.7004051
TGTCGCA537450.044.680813
CACACGC701850.044.67522
ACACCGC570200.044.6633032
Produced by FastQC (version 0.11.5)