FastQCFastQC Report
Thu 26 May 2016
SRR1161552_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161552_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24027498
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5009612.0849486700612774No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG409450.17040892064583668Illumina RNA PCR Primer (100% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA331490.13796276249820102No Hit
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC275960.11485174194999412Illumina RNA PCR Primer (100% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA118250.030.401741
TACCTAG56850.030.23632817
TAAGTAG58650.029.65861532
ACCTAGC58400.029.27679418
GTACCTA59050.029.18609216
TAGTTGG61150.028.22528336
ATGTACC63550.027.1601314
ATAAGTA65600.026.44622831
GTACTAT66850.026.1818926
CCTAGCT66700.025.83602719
TGTACTA72500.024.14150825
CTATAAG73700.023.84179329
TACGCGT24800.022.86656613
GTAGTTG81400.022.1159235
TATGTAC78900.021.96169313
CTAGCTG80500.021.79824420
TATAAGT82250.021.20201730
TAGCTGT84400.020.73766921
TGGTCGC56900.020.67817745
AAGTAGT87150.020.60510833
Produced by FastQC (version 0.11.5)