FastQCFastQC Report
Thu 26 May 2016
SRR1161552_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161552_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24027498
Sequences flagged as poor quality0
Sequence length51
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA756750.31495164415371085No Hit
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA528360.21989805180714198No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC512630.2133513859828435RNA PCR Primer, Index 6 (100% over 42bp)
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA485530.20207264193716715No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC456480.18998232774798274RNA PCR Primer, Index 6 (100% over 44bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431570.17961503940193854No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA396190.16489024366998178No Hit
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA277890.11565498829715853No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA261310.10875456112825396No Hit
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA254590.10595776555677998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA944450.045.031861
GCTTGCG539200.045.0096861
GACACTC785250.044.973771
GACACGA749450.044.970471
CGTGTTC800200.044.956511
TAACGAG945500.044.9341472
ACGACCG759900.044.933751
AGGTCGC338900.044.932862
GACACCG558350.044.9200821
ACACGAG749100.044.9101942
ACACTCA778400.044.908872
CTAACCG594450.044.9048081
GCCACGC526400.044.8873832
CGACCGC755150.044.8729362
AGCCACG529700.044.871711
ACACCGC556850.044.858832
GCTTGTC602500.044.8476031
ATGTGCG739550.044.8436241
ACGACAT470550.044.8374821
TGTGGAG515150.044.830082
Produced by FastQC (version 0.11.5)