FastQCFastQC Report
Thu 26 May 2016
SRR1161551_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161551_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23764721
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5185372.1819612357325804No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG436680.18375136825717417Illumina RNA PCR Primer (100% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA347770.1463387682943974No Hit
GTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTC286740.12065784403696556Illumina RNA PCR Primer (100% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA112550.030.7835921
TACCTAG54300.030.03934717
GTACCTA55900.029.18071216
ACCTAGC56750.028.86009218
TAAGTAG57950.027.95801232
TAGTTGG61700.026.40477636
ATGTACC62650.026.0771514
ATAAGTA62550.025.97187631
GTACTAT63700.025.95853226
CCTAGCT63650.025.8375419
CTATAAG71300.023.25559829
TGTACTA72200.023.0271125
TATGTAC74900.021.81238213
TGGTCGC54850.021.45147945
TAGCTGT78150.021.24513821
GTAGTTG79300.021.1119735
CTAGCTG79100.021.07530820
GGTGGTC172100.020.20974345
GGTGAGA114950.020.19831
TACGCGT24400.019.92104713
Produced by FastQC (version 0.11.5)