FastQCFastQC Report
Thu 26 May 2016
SRR1161551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161551_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23764721
Sequences flagged as poor quality0
Sequence length51
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730570.3074178737465506No Hit
GTCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510260.2147132297492573No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC505210.21258823110105102RNA PCR Primer, Index 6 (100% over 42bp)
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA461820.19433007439893782No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC454490.19124567042045224RNA PCR Primer, Index 6 (100% over 44bp)
CATCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA409620.17236474183728057No Hit
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA379160.15954742325819857No Hit
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA268030.11278482924331407No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245220.10318656802240599No Hit
TGATGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA244070.10270265743915108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGA928350.045.0477561
CGTGTTC794500.045.0136221
GCTTGCG526850.044.9789281
GACACGA753800.044.961161
GACACTC785750.044.9552761
ACACGAG752850.044.9430162
TAACGAG931400.044.9292952
AGCCACG523200.044.9220621
ACGACCG751650.044.9217641
CGACCGC747650.044.9147872
AGGTCGC338150.044.8987122
GACACCG545750.044.8921741
ACACTCA780550.044.8907622
CTAACCG592100.044.8893051
ACACCGC545350.044.887892
TAGGTCG339900.044.8732031
GCCACGC522500.044.865722
CTAACTC528150.044.8468631
ATGTGCG729600.044.845331
ACGACAT466950.044.8428231
Produced by FastQC (version 0.11.5)