FastQCFastQC Report
Thu 26 May 2016
SRR1161550_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161550_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42211075
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6449411.5278952265489567No Hit
CAGTCCGACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG692140.16397118528727353Illumina RNA PCR Primer (100% over 37bp)
GTAACTTTTGTGTATGTACCTAGCTGTACTATAAGTAGTTGGTTTGTATGA481980.11418330378934911No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA214200.031.855191
TACCTAG84150.027.51264217
ACCTAGC87000.026.50524918
GTACCTA88400.026.11365316
TAAGTAG93650.024.84563632
TAGTTGG93500.024.64482336
ATGTACC96950.023.8841114
GTACTAT100150.023.47429826
CCTAGCT103350.022.35564619
ATAAGTA107500.021.64346531
CTATAAG112300.020.75551429
TGGTCGC100700.020.64285545
TGTACTA114800.020.47866625
TACGCGT45650.019.17568213
TATGTAC122050.019.15665813
TAGCTGT130750.018.1869821
GTAGTTG131750.018.05351335
CTAGCTG131750.018.0318620
GTGGTCG158050.017.96354945
GGTGGTC284650.017.8854845
Produced by FastQC (version 0.11.5)