FastQCFastQC Report
Thu 26 May 2016
SRR1161550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161550_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42211075
Sequences flagged as poor quality0
Sequence length51
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA904300.21423287608761443No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC858250.20332341689947483RNA PCR Primer, Index 5 (100% over 42bp)
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA739310.1751459776847664No Hit
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA694780.1645966135664633No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC657610.1557908676810529RNA PCR Primer, Index 5 (100% over 44bp)
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA598740.1418442908644236No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530840.1257584650473839No Hit
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480780.11389901820789924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCGC752850.044.9737242
AGCCACG909500.044.968631
GACACTC1431550.044.9497221
CTAACGA1062950.044.9455341
GCTTGCG905450.044.938151
GACACGA1259200.044.916271
CTAACCG1133550.044.9135321
GCCACGC905350.044.913222
TAACGAG1062650.044.8945542
GACACCG963400.044.89421
ACACGAG1257150.044.8908582
ACACCGC959250.044.874492
ATGTGCG1853800.044.8677441
CTAACTC1084800.044.8634641
ACACTCA1422600.044.856822
GCTTGTC966000.044.8504031
AGCCATC1389350.044.8476941
ACGACAT754150.044.842371
CGACCGC1214500.044.8295752
ACGACCG1222000.044.82941
Produced by FastQC (version 0.11.5)