FastQCFastQC Report
Thu 26 May 2016
SRR1161549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1161549_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41751147
Sequences flagged as poor quality0
Sequence length51
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA868140.20793201202352596No Hit
GTCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC852460.2041764265781728RNA PCR Primer, Index 5 (100% over 42bp)
GACACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA712190.17057974479120297No Hit
CGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA669800.16042673031234328No Hit
GTCGGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC656940.15734657541264674RNA PCR Primer, Index 5 (100% over 44bp)
ATGTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA566600.13570884651384546No Hit
AGCCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA504470.12082781821538939No Hit
TAGGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460750.11035624961393276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACACGA1248450.044.9815561
AGGTCGC778050.044.9533352
GACACTC1410250.044.9434471
CTAACGA1049200.044.940611
AGCCACG900950.044.9351651
GCCACGC898400.044.9146352
GCTTGCG892250.044.9133871
CTAACCG1111000.044.9065971
ATGTGCG1806650.044.8931541
GACACCG935950.044.8857461
ACACGAG1247300.044.885642
TAACGAG1049150.044.884722
ACACCGC933600.044.8731422
ACACTCA1403950.044.8512272
AGCCATC1367800.044.8414731
CTAACTC1064400.044.8411331
CGACCGC1205100.044.8315242
ACGACAT743050.044.8283041
CGTGTTC1118500.044.821181
GCTTGTC961450.044.8186071
Produced by FastQC (version 0.11.5)