FastQCFastQC Report
Thu 26 May 2016
SRR2048278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18516247
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC393410.21246746168378508No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT391960.21168436562765663No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT385490.208190137018587No Hit
GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC281100.1518126216397956No Hit
GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG270650.14616892937321477No Hit
CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC263480.14229665439222106No Hit
CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA254100.13723083300843847No Hit
CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA253900.1371228197593173No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC241410.13037739235170065No Hit
CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA216280.11680552759962644No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC203500.10990348098078406No Hit
CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA203370.10983327236885532No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT200240.1081428650201091No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT197290.10654966959557192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA238250.080.1958162
TCGGGAA111700.067.999283
AAGCGGC78600.056.879769
CGGGAGA279350.051.435174
ATCCGAA43400.051.188792
CGGGAAA168350.050.216594
AAGGGGC207100.049.2476779
CGGAAAA114650.048.316254
GATCCGA46850.048.103311
GATTGGG94050.047.265721
CGAAAAG54700.046.34195
AGAGCGG149750.045.289228
GATCGGG455850.042.6130071
GGGCGAT96750.042.2039681
AACTCGG120200.038.95118734-35
GAACGGA68200.038.2842031
CGGATTA63900.038.2138848-49
GGGAGAG462150.038.0972635
ATTTCGG89350.037.7791734-35
TCCGAAA37600.037.750343
Produced by FastQC (version 0.11.5)