##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048278_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18516247
Sequences flagged as poor quality	0
Sequence length	101
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.601236200834865	31.0	30.0	34.0	16.0	34.0
2	28.96859714606313	31.0	30.0	34.0	16.0	34.0
3	29.100869036797793	31.0	31.0	34.0	20.0	34.0
4	32.60295323344952	37.0	35.0	37.0	22.0	37.0
5	32.57165607047692	37.0	35.0	37.0	22.0	37.0
6	32.55311964676211	37.0	35.0	37.0	23.0	37.0
7	32.51214649491336	37.0	35.0	37.0	22.0	37.0
8	32.59507199272077	37.0	35.0	37.0	23.0	37.0
9	34.039252068737255	39.0	35.0	39.0	22.0	39.0
10-11	34.02716757342889	39.0	35.0	39.0	22.0	39.0
12-13	33.96086982421438	39.0	35.0	39.0	20.5	39.0
14-15	35.062414780921856	40.0	36.0	41.0	18.5	41.0
16-17	35.040068702907234	40.0	36.0	41.0	18.5	41.0
18-19	34.94724662616566	40.0	36.0	41.0	17.5	41.0
20-21	34.89139772762807	40.0	36.0	41.0	18.0	41.0
22-23	34.820383984940364	40.0	36.0	41.0	17.5	41.0
24-25	34.70413488759358	40.0	35.5	41.0	16.0	41.0
26-27	34.60216122630034	40.0	35.0	41.0	15.0	41.0
28-29	34.47558379405935	40.0	35.0	41.0	13.0	41.0
30-31	34.36834640410662	40.0	35.0	41.0	9.5	41.0
32-33	34.26954590203944	39.0	35.0	41.0	9.0	41.0
34-35	33.91147536539127	39.0	34.0	41.0	5.0	41.0
36-37	33.83746727941143	39.0	34.0	41.0	7.5	41.0
38-39	33.809171534598775	39.0	34.0	41.0	5.5	41.0
40-41	33.60756820753147	38.5	33.5	40.5	2.0	41.0
42-43	33.155996703867686	38.0	33.0	40.0	2.0	41.0
44-45	32.930069576194356	38.0	33.0	40.0	2.0	41.0
46-47	32.70692546389125	38.0	32.5	40.0	2.0	41.0
48-49	32.45867510300548	37.5	32.0	40.0	2.0	41.0
50-51	32.4249780746606	37.0	32.0	40.0	2.0	41.0
52-53	32.294917377155315	37.0	32.0	40.0	2.0	41.0
54-55	32.19777371731971	37.0	32.0	40.0	2.0	41.0
56-57	31.927413314371968	36.0	32.0	40.0	2.0	41.0
58-59	31.64090638885947	35.5	31.0	39.0	2.0	41.0
60-61	31.339069763975388	35.0	31.0	39.0	2.0	41.0
62-63	31.011989389642512	35.0	31.0	39.0	2.0	40.5
64-65	30.734724590787756	35.0	31.0	38.0	2.0	40.0
66-67	30.429969636935606	35.0	30.5	37.0	2.0	40.0
68-69	30.08147018129538	35.0	30.0	36.5	2.0	39.0
70-71	29.79831914642314	35.0	30.0	36.0	2.0	39.0
72-73	29.54995315735419	35.0	30.0	36.0	2.0	38.5
74-75	29.2907899748799	34.0	29.5	35.0	2.0	37.0
76-77	28.995474001832015	34.0	29.0	35.0	2.0	37.0
78-79	28.791860224158818	34.0	29.0	35.0	2.0	36.5
80-81	28.6402082992304	34.0	29.0	35.0	2.0	36.0
82-83	28.4474490159912	34.0	29.0	35.0	2.0	36.0
84-85	28.34639244118962	34.0	29.0	35.0	2.0	35.5
86-87	28.53725587588025	34.0	29.0	35.0	2.0	35.0
88-89	28.48388474727087	34.0	29.0	35.0	2.0	35.0
90-91	28.365504764545427	34.0	29.0	35.0	2.0	35.0
92-93	28.248134867719145	34.0	29.0	35.0	2.0	35.0
94-95	28.129096085184003	34.0	29.0	35.0	2.0	35.0
96-97	27.95253209249153	34.0	29.0	35.0	2.0	35.0
98-99	27.76091359118292	34.0	29.0	35.0	2.0	35.0
100-101	27.081941929160912	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4491434101957239
1101	2	0.4973612161194758
1101	3	0.5211918587128856
1101	4	0.48879310183892244
1101	5	0.511739401429864
1101	6	0.46528411243094325
1101	7	0.4996733287920634
1101	8	0.44374138989510925
1101	9	0.5752294242087785
1101	10-11	0.5405071606393221
1101	12-13	0.5197466951145273
1101	14-15	0.6604532087564365
1101	16-17	0.6744908468409392
1101	18-19	0.6880644719172864
1101	20-21	0.6776908706125795
1101	22-23	0.6953352519806018
1101	24-25	0.6580456673227886
1101	26-27	0.6931852801350757
1101	28-29	0.7090176200369953
1101	30-31	0.6827582826805241
1101	32-33	0.6744272935141993
1101	34-35	0.7765219356259436
1101	36-37	0.7566172136742821
1101	38-39	0.7532348832994273
1101	40-41	0.7346066081190372
1101	42-43	0.7213105127290049
1101	44-45	0.7507386045292321
1101	46-47	0.7696749788120201
1101	48-49	0.7704004675448601
1101	50-51	0.7218983662503078
1101	52-53	0.6747220950960937
1101	54-55	0.6159379328791985
1101	56-57	0.6128946336439469
1101	58-59	0.630364736050808
1101	60-61	0.5901136394848159
1101	62-63	0.6018437040897524
1101	64-65	0.5804844463866026
1101	66-67	0.5763651700947143
1101	68-69	0.5050671987719504
1101	70-71	0.5264551237957598
1101	72-73	0.5010235224635515
1101	74-75	0.48375683971899264
1101	76-77	0.5338997218778339
1101	78-79	0.458082150777706
1101	80-81	0.41053607932484937
1101	82-83	0.383690217769626
1101	84-85	0.4328020225238056
1101	86-87	0.48060239686746087
1101	88-89	0.46779459307559534
1101	90-91	0.49791892441694685
1101	92-93	0.5068185827417615
1101	94-95	0.41423389480150163
1101	96-97	0.39671035902634344
1101	98-99	0.4414688259631312
1101	100-101	0.46651205080302205
1102	1	0.4745469601224599
1102	2	0.5110924257206442
1102	3	0.49755362918377344
1102	4	0.4958011964370783
1102	5	0.5431248450539954
1102	6	0.4947403651702942
1102	7	0.4911219995724281
1102	8	0.47846847838841455
1102	9	0.5860450865422848
1102	10-11	0.5585907372318815
1102	12-13	0.5539213496518514
1102	14-15	0.6882283666715026
1102	16-17	0.6512383329100118
1102	18-19	0.7071056614547899
1102	20-21	0.6999192060319999
1102	22-23	0.7017120081553188
1102	24-25	0.6932069189081247
1102	26-27	0.708461600743469
1102	28-29	0.6971909981250164
1102	30-31	0.695653669527033
1102	32-33	0.6964908736010216
1102	34-35	0.7702061952800321
1102	36-37	0.7369788409077032
1102	38-39	0.7898809023210802
1102	40-41	0.7656545774690358
1102	42-43	0.7884598494525505
1102	44-45	0.7782946320358732
1102	46-47	0.8381291480877238
1102	48-49	0.8662142599264087
1102	50-51	0.7867542389511826
1102	52-53	0.6962976817204591
1102	54-55	0.6579587742414574
1102	56-57	0.6755501315920114
1102	58-59	0.6898725836703932
1102	60-61	0.6653701755909829
1102	62-63	0.6608466451042041
1102	64-65	0.6473473394688405
1102	66-67	0.6247043349418782
1102	68-69	0.6660820739078055
1102	70-71	0.6060674740201044
1102	72-73	0.6063052067595116
1102	74-75	0.5653920260344094
1102	76-77	0.5865203000223964
1102	78-79	0.5646474642262085
1102	80-81	0.5268667403629195
1102	82-83	0.5772708288913471
1102	84-85	0.5349159505246348
1102	86-87	0.5750096965397269
1102	88-89	0.5923268463688878
1102	90-91	0.6121253755983282
1102	92-93	0.5763707192581116
1102	94-95	0.5579829092577633
1102	96-97	0.6020801975131249
1102	98-99	0.6149414921666008
1102	100-101	0.6243924656636182
1103	1	0.3721960986803623
1103	2	0.38360713210963837
1103	3	0.4037936851242243
1103	4	0.445697777005563
1103	5	0.465668714320131
1103	6	0.44071832712809567
1103	7	0.46737142743101856
1103	8	0.45100356627338556
1103	9	0.5281463047976942
1103	10-11	0.476474571687433
1103	12-13	0.48628418105959526
1103	14-15	0.5802233417613039
1103	16-17	0.5729562095715579
1103	18-19	0.5928916438005203
1103	20-21	0.5702629935284946
1103	22-23	0.5098003369336297
1103	24-25	0.5509938541137203
1103	26-27	0.5716772416379783
1103	28-29	0.5550072874367586
1103	30-31	0.5603518935569411
1103	32-33	0.5525966846618289
1103	34-35	0.5987260441471776
1103	36-37	0.6255331774553312
1103	38-39	0.652589670445316
1103	40-41	0.610559993090078
1103	42-43	0.5895161764788952
1103	44-45	0.5777744987011957
1103	46-47	0.6071346120003085
1103	48-49	0.6198711349793058
1103	50-51	0.5782910764814986
1103	52-53	0.5584341114475038
1103	54-55	0.49572131393011176
1103	56-57	0.4825572459957286
1103	58-59	0.5046698940220722
1103	60-61	0.4983497673471966
1103	62-63	0.49841424410124446
1103	64-65	0.49879979509649885
1103	66-67	0.4817806960638329
1103	68-69	0.43835646347877244
1103	70-71	0.4608805656738433
1103	72-73	0.44566603070520827
1103	74-75	0.42685324919352396
1103	76-77	0.46668233974934736
1103	78-79	0.43226986151465496
1103	80-81	0.375270717820662
1103	82-83	0.4080888616721339
1103	84-85	0.4002936428622803
1103	86-87	0.4290377643565755
1103	88-89	0.41611782707702716
1103	90-91	0.43987171225073496
1103	92-93	0.42400745323625344
1103	94-95	0.4088435391620884
1103	96-97	0.4438370988616782
1103	98-99	0.43365495500219353
1103	100-101	0.43141117351489555
1104	1	0.19106385602940534
1104	2	0.1861372901499294
1104	3	0.2258213104841431
1104	4	0.2614832765696491
1104	5	0.28157289285469744
1104	6	0.17610626184237788
1104	7	0.19378325445335065
1104	8	0.1661533410743843
1104	9	0.26964378117184395
1104	10-11	0.20062203952446112
1104	12-13	0.22904274276101688
1104	14-15	0.2848975146303232
1104	16-17	0.3064890981982984
1104	18-19	0.3316794723181431
1104	20-21	0.3527149010584978
1104	22-23	0.2991264085368215
1104	24-25	0.2915996213296097
1104	26-27	0.3385463093063308
1104	28-29	0.3166244872856723
1104	30-31	0.31082361821138704
1104	32-33	0.299542641427756
1104	34-35	0.31894894071603375
1104	36-37	0.33290705437068624
1104	38-39	0.35228215609389224
1104	40-41	0.3023248366925415
1104	42-43	0.2743724973143955
1104	44-45	0.31942315332792504
1104	46-47	0.34804483766497185
1104	48-49	0.351863460621459
1104	50-51	0.3193417281487001
1104	52-53	0.28745655244433266
1104	54-55	0.2341623217506381
1104	56-57	0.2656066714213594
1104	58-59	0.28273067964451926
1104	60-61	0.22093929074279828
1104	62-63	0.2582130201879842
1104	64-65	0.2728719001703972
1104	66-67	0.2658389735824791
1104	68-69	0.24049598338191913
1104	70-71	0.2589883151842507
1104	72-73	0.27540398947865796
1104	74-75	0.2782728372384433
1104	76-77	0.26434156682910626
1104	78-79	0.279407547876211
1104	80-81	0.27731634551923534
1104	82-83	0.2684610318092666
1104	84-85	0.23661165529026462
1104	86-87	0.309626347374941
1104	88-89	0.3381469561350734
1104	90-91	0.27117869109160253
1104	92-93	0.26822440809555914
1104	94-95	0.280244930842791
1104	96-97	0.27879151578898487
1104	98-99	0.2944853488482231
1104	100-101	0.27452137421493816
1105	1	0.22624475964881086
1105	2	0.22902821483475932
1105	3	0.22483714154123646
1105	4	0.2646312209960513
1105	5	0.24234462934155943
1105	6	0.15491547118299565
1105	7	0.18930995785672877
1105	8	0.16910666625460635
1105	9	0.24552182046554805
1105	10-11	0.15024320408292624
1105	12-13	0.13600052804572726
1105	14-15	0.16074376984494876
1105	16-17	0.18400158158188162
1105	18-19	0.2354035887753696
1105	20-21	0.1916394005364026
1105	22-23	0.15880344370045663
1105	24-25	0.18216900706264738
1105	26-27	0.18380005517027342
1105	28-29	0.16585501230627386
1105	30-31	0.18487604830590953
1105	32-33	0.164753349381364
1105	34-35	0.19263764199917688
1105	36-37	0.2201910230266364
1105	38-39	0.2658519788377305
1105	40-41	0.1848384194134809
1105	42-43	0.14024093726167308
1105	44-45	0.1683210323305886
1105	46-47	0.2287187946285414
1105	48-49	0.25507431256055213
1105	50-51	0.20836080867157847
1105	52-53	0.19462226073537892
1105	54-55	0.18326488586173895
1105	56-57	0.15973631619865358
1105	58-59	0.10278650248014998
1105	60-61	0.1716306836145698
1105	62-63	0.18894693152492792
1105	64-65	0.1835326483580566
1105	66-67	0.16013004513006024
1105	68-69	0.10754821247821766
1105	70-71	0.16627870002117717
1105	72-73	0.17045505020202611
1105	74-75	0.15691886368192698
1105	76-77	0.12971360862155734
1105	78-79	0.1518421511324064
1105	80-81	0.1603033938277605
1105	82-83	0.1757428600183033
1105	84-85	0.114583093779558
1105	86-87	0.18006172344346894
1105	88-89	0.14802415480700404
1105	90-91	0.12994571174883518
1105	92-93	0.0644101934951884
1105	94-95	0.15488550600553097
1105	96-97	0.1792207992721373
1105	98-99	0.1379568659103576
1105	100-101	0.08139581234033955
1106	1	0.10423261479201074
1106	2	0.08917153079114115
1106	3	0.1648850315185868
1106	4	0.13545798713420254
1106	5	0.1780416466035888
1106	6	0.05397793317968791
1106	7	0.12376600855147046
1106	8	0.11169332362702278
1106	9	0.1476314216413641
1106	10-11	0.07346755955409634
1106	12-13	0.1176707584789014
1106	14-15	0.13321441928336952
1106	16-17	0.1509376968795877
1106	18-19	0.18942202557209953
1106	20-21	0.20263605440938903
1106	22-23	0.2136102630053287
1106	24-25	0.16864528299219472
1106	26-27	0.15030569478778943
1106	28-29	0.14234977984818187
1106	30-31	0.15294876006769442
1106	32-33	0.17061829988260513
1106	34-35	0.15478836965281317
1106	36-37	0.16984716541913514
1106	38-39	0.1764543481413341
1106	40-41	0.17512014101991724
1106	42-43	0.20453035715797796
1106	44-45	0.20724835753465243
1106	46-47	0.2574229492765596
1106	48-49	0.1770963600997959
1106	50-51	0.18770369481429583
1106	52-53	0.14776767478569042
1106	54-55	0.10256228970800407
1106	56-57	0.13723028416141858
1106	58-59	0.1927064353679313
1106	60-61	0.17255453837362467
1106	62-63	0.09650741552033892
1106	64-65	0.1060146794836605
1106	66-67	0.1533361615031552
1106	68-69	0.13139565717420965
1106	70-71	0.12076760099265371
1106	72-73	0.19170231820900696
1106	74-75	0.1349380637648494
1106	76-77	0.1948288525223134
1106	78-79	0.11894920366755102
1106	80-81	0.1019305902564227
1106	82-83	0.1566382501218655
1106	84-85	0.13337871606222862
1106	86-87	0.14019093732942878
1106	88-89	0.14501237213284313
1106	90-91	0.13942662948560525
1106	92-93	0.1275008294539255
1106	94-95	0.13042599958430046
1106	96-97	0.1498044811932644
1106	98-99	0.12434507840631781
1106	100-101	0.12303602708367123
1107	1	0.22737651280728244
1107	2	0.1847769734145963
1107	3	0.186411157802425
1107	4	0.22982320726114125
1107	5	0.23533798288740115
1107	6	0.1651136209520132
1107	7	0.19661567793124135
1107	8	0.21300241640854978
1107	9	0.250710554307652
1107	10-11	0.1693543356640248
1107	12-13	0.1822225697072355
1107	14-15	0.1597050053764164
1107	16-17	0.15189704692740236
1107	18-19	0.21610397839661744
1107	20-21	0.20043347868050176
1107	22-23	0.2262302291528968
1107	24-25	0.1977713618514727
1107	26-27	0.1602071994007659
1107	28-29	0.14640970159106814
1107	30-31	0.1706313432827926
1107	32-33	0.10770843215789938
1107	34-35	0.14290049026664775
1107	36-37	0.16451667253175373
1107	38-39	0.2293592688020425
1107	40-41	0.14294484595163226
1107	42-43	0.1350277421105588
1107	44-45	0.16263430219000696
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2216	92-93	-1.0128124138731458
2216	94-95	-1.0682294885629773
2216	96-97	-1.0524092140644612
2216	98-99	-1.0481694784511397
2216	100-101	-1.2635465140210584
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1453570.0
3	67495.0
4	44947.0
5	40205.0
6	40995.0
7	42156.0
8	44875.0
9	48544.0
10	50081.0
11	52599.0
12	53158.0
13	54558.0
14	55273.0
15	57713.0
16	58369.0
17	58763.0
18	59593.0
19	59344.0
20	60518.0
21	65010.0
22	73153.0
23	87727.0
24	110457.0
25	138294.0
26	172544.0
27	211683.0
28	261314.0
29	322600.0
30	402156.0
31	509845.0
32	656376.0
33	850272.0
34	1147847.0
35	1676572.0
36	2788200.0
37	3861943.0
38	2492972.0
39	284356.0
40	170.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.95200424194622	16.01712046192301	7.9364485569276635	32.09442673920311
2	32.3021858179665	20.02394111862522	22.05416181996623	25.61971124344205
3	31.10521233694107	16.955819694588897	20.61989517197695	31.319072796493085
4	33.739250183846856	19.009951875627323	18.21910890120549	29.031689039320323
5	36.52005468599517	21.003801219587242	19.388247745597862	23.08789634881973
6	33.81666328696381	25.53799191575077	19.711951981181812	20.93339281610361
7	26.888157188175388	23.15630509453832	29.57209568335154	20.38344203393475
8	23.10597799474444	25.324668126069618	27.654368201837364	23.91498567734858
9	24.269133759296228	21.202377225336324	31.077435693067244	23.4510533223002
10-11	27.290845936083237	27.62370387527139	23.13824071026714	21.94720947837823
12-13	28.436742979822082	23.405921631141467	23.365910061726535	24.79142532730992
14-15	26.496451189723103	23.43800186073296	25.026452682917228	25.03909426662671
16-17	28.705347857176516	22.937817796716143	24.535588892694264	23.821245453413077
18-19	28.951580952019363	22.60057385125704	24.931633992007793	23.51621120471581
20-21	28.0444954214416	24.447169692574683	23.684642332774416	23.8236925532093
22-23	30.56618234363258	23.224016003559328	22.069798143559215	24.140003509248878
24-25	26.896510728619127	25.53182259754745	23.208547142659295	24.363119531174128
26-27	25.967535187733283	26.452539686034836	22.725476790479018	24.85444833575286
28-29	26.82959866894077	25.4814839373118	22.24467864712639	25.444238746621046
30-31	28.79959037339613	23.5959340826835	22.958516245019137	24.64595929890124
32-33	25.81924507251163	24.914603434662947	24.369526282443964	24.896625210381462
34-35	26.892411939089282	25.533081835135775	22.789337433148248	24.78516879262669
36-37	25.083160873304973	23.81527038468612	25.095780856094528	26.005787885914383
38-39	24.503604276505285	22.91518663651725	25.308794761337545	27.27241432563992
40-41	29.464153421543383	23.166862037863424	22.512995247188982	24.855989293404214
42-43	29.55275978381289	22.847026214357776	23.335450033274675	24.26476396855466
44-45	30.684273545216257	22.71093059846781	22.67890691446288	23.925888941853053
46-47	28.663777064858927	22.830819140624122	22.977487708479867	25.527916086037084
48-49	25.852440507479084	23.146288333240495	22.683505765277776	28.31776539400264
50-51	27.911611233755963	23.6665956151834	23.224184536862808	25.197608614197826
52-53	25.251968335054453	25.569437848414733	24.7149034353421	24.463690381188716
54-55	25.35876340457327	24.807198204214377	23.6661862767542	26.16785211445815
56-57	23.947019727088193	23.659655576933268	27.489190974771326	24.904133721207213
58-59	24.034789938494207	26.91108028134171	24.589793416649457	24.464336363514633
60-61	24.238642083538913	29.06336841950926	22.557775110417698	24.14021438653413
62-63	24.446797273578063	29.042973340554173	22.473552454917854	24.03667693094991
64-65	25.416397076312556	28.036782861934956	22.553359686787804	23.993460374964684
66-67	25.3949333252844	27.765590781546173	22.24861656226311	24.59085933090632
68-69	25.54388129569905	26.940520055482896	22.843321395521745	24.67227725329631
70-71	25.72529577361154	26.218438224437218	23.27200026943536	24.784265732515884
72-73	26.50602425935476	26.08809497793661	22.870361981469472	24.53551878123916
74-75	27.20594367389917	26.253508543353615	22.33407308168364	24.20647470106357
76-77	27.06147640875587	25.367771930645986	22.985313899443995	24.585437761154147
78-79	26.443784905576166	25.080305732105053	23.544030981646742	24.93187838067204
80-81	27.467803218812612	25.087218548634883	23.461359535050782	23.983618697501722
82-83	27.64687114928185	24.709763287720587	23.561507959317346	24.081857603680216
84-85	27.74123479302761	25.0521486808947	22.34605898989654	24.86055753618115
86-87	27.977594496695783	25.17442112206394	22.293857667106792	24.554126714133485
88-89	27.79619664730389	24.636029414648114	22.214692768775954	25.353081169272045
90-91	27.954157296708537	24.37402494232495	22.93502821848087	24.736789542485642
92-93	27.840898979000944	25.12908068973221	22.86320603279022	24.16681429847663
94-95	27.904363082434593	25.89076194702022	22.640763549772938	23.564111420772253
96-97	27.98600412658187	25.618966317127796	22.9429420542741	23.452087502016237
98-99	27.637329556608393	26.142318225162175	22.68638875807571	23.53396346015372
100-101	27.449129085178757	26.65534121112334	22.392191493225347	23.503338210472556
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2098.0
1	2464.5
2	2915.0
3	2856.5
4	2535.0
5	2235.5
6	2041.0
7	1941.5
8	1785.0
9	1631.0
10	1548.5
11	1422.0
12	1380.0
13	1463.0
14	1514.0
15	1680.5
16	2186.0
17	2838.0
18	3254.5
19	3740.5
20	4536.5
21	5688.0
22	6932.0
23	8469.5
24	10425.0
25	12497.5
26	15616.5
27	19415.0
28	23164.5
29	29151.0
30	41182.0
31	47250.0
32	49966.0
33	63342.5
34	75663.0
35	95408.0
36	127945.5
37	148875.5
38	180013.5
39	235976.5
40	304157.0
41	387130.0
42	467630.5
43	526535.0
44	605568.0
45	694806.0
46	787074.0
47	860288.0
48	880265.0
49	875208.5
50	845427.0
51	801435.0
52	761442.0
53	735925.0
54	675167.5
55	620713.0
56	603538.0
57	565321.5
58	521928.5
59	510429.0
60	485525.5
61	443802.5
62	434563.0
63	441367.5
64	402392.0
65	312633.5
66	233662.0
67	199468.0
68	200040.5
69	161953.0
70	106837.5
71	82187.0
72	68434.0
73	57769.0
74	52800.0
75	60125.5
76	62841.0
77	59063.5
78	56040.5
79	45292.0
80	35222.0
81	32793.0
82	31515.5
83	28372.0
84	24562.5
85	23490.5
86	24561.5
87	21978.5
88	15871.5
89	10417.0
90	7322.5
91	5499.0
92	4171.5
93	3201.0
94	2143.5
95	1342.5
96	929.5
97	704.0
98	538.5
99	383.0
100	299.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5308095101561348
2	0.11722677927119897
3	0.1412435252132897
4	0.12936746847241776
5	0.12832514061839853
6	0.12689936572999916
7	0.13052861090047027
8	0.13075003806116867
9	0.1365179455642388
10-11	0.15543376581658258
12-13	0.15037874575771212
14-15	0.13871061452139843
16-17	0.12632959584088505
18-19	0.14372512961184844
20-21	0.1568433387176138
22-23	0.13561333460284905
24-25	0.1517775173338312
26-27	0.17608319871732106
28-29	0.1437197289493924
30-31	0.1680659153013027
32-33	0.1469682274167114
34-35	0.42309599780128226
36-37	0.13896174532560512
38-39	0.14223454677397637
40-41	0.14626884162865184
42-43	0.255159698398925
44-45	0.14477285812832374
46-47	0.1786404123902646
48-49	0.28295420772902846
50-51	0.13480323523444032
52-53	0.16065620641159084
54-55	0.17446029964927556
56-57	0.18428410465684542
58-59	0.17838118059237382
60-61	0.17895905147517205
62-63	0.20660774291896192
64-65	0.16129618491263376
66-67	0.1583960291737305
68-69	0.15915482224880667
70-71	0.1790292600871008
72-73	0.18326337945265042
74-75	0.17421726983875296
76-77	0.16804431265147846
78-79	0.1680119086767421
80-81	0.1616256253224533
82-83	0.16992914384864277
84-85	0.1564166863835852
86-87	0.15519073600605998
88-89	0.15182072263347968
90-91	0.1732748542401708
92-93	0.16170663525929418
94-95	0.17510297848154652
96-97	0.17250525984018253
98-99	0.17556203479031146
100-101	0.1454101362981386
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.8516247E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.84702803518579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.01935477112985	37.28520733482379
2	8.47602068207075	7.263445915829999
3	1.896942199150985	2.438350067644477
4	0.734676496525984	1.2591481777376363
5	0.40738106013099784	0.8727533852218284
6	0.2555721077098647	0.6570303158433657
7	0.16912593562604894	0.5072580593672442
8	0.11193814022864598	0.3836973306066682
9	0.09787662473098341	0.3774350235454024
>10	0.6036297947956415	5.138533240875371
>50	0.0860029401225134	2.6011013342143787
>100	0.10111949964199975	9.464265143251897
>500	0.019300980824094458	5.853166441318472
>1k	0.01867814745128148	16.21858316961238
>5k	0.0017221505372316352	4.949388493519399
>10k+	6.584693230591547E-4	4.730636566587772
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC	39341	0.21246746168378508	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT	39196	0.21168436562765663	No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT	38549	0.208190137018587	No Hit
GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC	28110	0.1518126216397956	No Hit
GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG	27065	0.14616892937321477	No Hit
CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC	26348	0.14229665439222106	No Hit
CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA	25410	0.13723083300843847	No Hit
CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA	25390	0.1371228197593173	No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC	24141	0.13037739235170065	No Hit
CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA	21628	0.11680552759962644	No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC	20350	0.10990348098078406	No Hit
CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA	20337	0.10983327236885532	No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT	20024	0.1081428650201091	No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT	19729	0.10654966959557192	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.752582961331203E-4	5.400662456058185E-6	0.0	0.0	0.0
2	5.56268232973993E-4	4.320529964846548E-5	0.0	0.0	0.0
3	5.778708827982258E-4	5.940728701664004E-5	0.0	5.400662456058185E-6	0.0
4	6.048741950785167E-4	6.480794947269822E-5	0.0	2.160264982423274E-5	0.0
5	7.29089431567855E-4	7.560927438481459E-5	0.0	2.160264982423274E-5	0.0
6	8.100993684087278E-4	8.100993684087278E-5	0.0	2.160264982423274E-5	0.0
7	0.0010855331536676952	8.100993684087278E-5	0.0	2.160264982423274E-5	0.0
8	0.001425774888399361	8.100993684087278E-5	0.0	2.160264982423274E-5	0.0
9	0.0016255993992735137	8.100993684087278E-5	0.0	2.160264982423274E-5	0.0
10-11	0.0024735034048746487	8.641059929693096E-5	0.0	2.4302981052261834E-5	0.0
12-13	0.0032890034357394345	8.641059929693096E-5	5.400662456058185E-6	2.7003312280290926E-5	0.0
14-15	0.003737258419592264	1.0261258666510552E-4	5.400662456058185E-6	2.7003312280290926E-5	0.0
16-17	0.004263823009057937	1.2691556771736735E-4	1.6201987368174556E-5	2.7003312280290926E-5	0.0
18-19	0.004744481967647115	1.2961589894539645E-4	1.6201987368174556E-5	3.5104305964378204E-5	0.0
20-21	0.0060946475816616615	1.2961589894539645E-4	1.6201987368174556E-5	4.320529964846548E-5	0.0
22-23	0.011832851441223483	1.3231623017342554E-4	1.6201987368174556E-5	4.5905630876494574E-5	0.0
24-25	0.025153585389090995	1.431175550855419E-4	2.160264982423274E-5	5.6706955788610945E-5	0.0
26-27	0.04387768212424473	1.45817886313571E-4	2.160264982423274E-5	6.210761824466913E-5	0.0
28-29	0.07415649618413493	1.45817886313571E-4	2.160264982423274E-5	6.750828070072732E-5	0.0
30-31	0.11855804256661731	1.5661921122568737E-4	2.4302981052261834E-5	7.29089431567855E-5	0.0
32-33	0.17122800316932474	1.5661921122568737E-4	2.970364350832002E-5	8.100993684087278E-5	0.0
34-35	0.2306082868736845	1.5661921122568737E-4	3.240397473634911E-5	8.100993684087278E-5	0.0
36-37	0.3017890180445314	1.6201987368174556E-4	3.240397473634911E-5	8.100993684087278E-5	0.0
38-39	0.3843489450102928	1.809221922779492E-4	3.240397473634911E-5	8.371026806890187E-5	0.0
40-41	0.47708372004326793	1.836225235059783E-4	3.240397473634911E-5	8.641059929693096E-5	0.0
42-43	0.5792075467561002	1.863228547340074E-4	3.240397473634911E-5	8.911093052496006E-5	0.0
44-45	0.6863539895530666	1.9172351719006558E-4	3.240397473634911E-5	9.181126175298915E-5	0.0
46-47	0.8000055302783551	2.0792550455824013E-4	3.240397473634911E-5	9.181126175298915E-5	0.0
48-49	0.9310607057682909	2.160264982423274E-4	3.240397473634911E-5	9.721192420904733E-5	0.0
50-51	1.0774294596523797	2.241274919264147E-4	3.240397473634911E-5	9.721192420904733E-5	0.0
52-53	1.238663536946769	2.4302981052261834E-4	3.240397473634911E-5	9.991225543707643E-5	0.0
54-55	1.41643174235038	2.484304729786765E-4	3.240397473634911E-5	1.0261258666510552E-4	2.7003312280290926E-6
56-57	1.6282106195710178	2.565314666627638E-4	3.240397473634911E-5	1.0261258666510552E-4	5.400662456058185E-6
58-59	1.8728768308178219	2.7813411648699657E-4	3.240397473634911E-5	1.0261258666510552E-4	5.400662456058185E-6
60-61	2.1407145843323434	2.862351101710838E-4	3.240397473634911E-5	1.0261258666510552E-4	5.400662456058185E-6
62-63	2.4515578129844564	3.2133941613546205E-4	3.240397473634911E-5	1.080132491211637E-4	5.400662456058185E-6
64-65	2.810788276911622	3.5374339087181116E-4	3.240397473634911E-5	1.1611424280525098E-4	5.400662456058185E-6
66-67	3.214039540518119	3.618443845558984E-4	3.240397473634911E-5	1.1881457403328007E-4	5.400662456058185E-6
68-69	3.688152896210555	3.618443845558984E-4	3.240397473634911E-5	1.2421523648933826E-4	8.100993684087278E-6
70-71	4.205514756851105	3.726457094680148E-4	3.240397473634911E-5	1.3501656140145463E-4	1.080132491211637E-5
72-73	4.740137134701217	3.726457094680148E-4	3.240397473634911E-5	1.485182175416001E-4	1.3501656140145463E-5
74-75	5.313706389853192	3.726457094680148E-4	3.240397473634911E-5	1.5661921122568737E-4	1.6201987368174556E-5
76-77	5.94233809907591	3.726457094680148E-4	3.240397473634911E-5	1.5931954245371646E-4	1.6201987368174556E-5
78-79	6.635178284238702	3.726457094680148E-4	3.240397473634911E-5	1.6472020490977465E-4	1.6201987368174556E-5
80-81	7.393552807974531	3.726457094680148E-4	3.240397473634911E-5	1.6742053613780374E-4	1.6201987368174556E-5
82-83	8.202415424680822	3.726457094680148E-4	3.240397473634911E-5	1.7012086736583283E-4	1.6201987368174556E-5
84-85	9.058266505086047	3.753460406960439E-4	3.240397473634911E-5	1.809221922779492E-4	1.6201987368174556E-5
86-87	9.902835601620566	3.8074670315210203E-4	3.240397473634911E-5	1.8902318596203648E-4	2.160264982423274E-5
88-89	10.745830405049144	3.8884769683618934E-4	3.240397473634911E-5	1.9712417964612376E-4	2.160264982423274E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGA	23825	0.0	80.195816	2
TCGGGAA	11170	0.0	67.99928	3
AAGCGGC	7860	0.0	56.87976	9
CGGGAGA	27935	0.0	51.43517	4
ATCCGAA	4340	0.0	51.18879	2
CGGGAAA	16835	0.0	50.21659	4
AAGGGGC	20710	0.0	49.247677	9
CGGAAAA	11465	0.0	48.31625	4
GATCCGA	4685	0.0	48.10331	1
GATTGGG	9405	0.0	47.26572	1
CGAAAAG	5470	0.0	46.3419	5
AGAGCGG	14975	0.0	45.28922	8
GATCGGG	45585	0.0	42.613007	1
GGGCGAT	9675	0.0	42.203968	1
AACTCGG	12020	0.0	38.951187	34-35
GAACGGA	6820	0.0	38.284203	1
CGGATTA	6390	0.0	38.21388	48-49
GGGAGAG	46215	0.0	38.097263	5
ATTTCGG	8935	0.0	37.77917	34-35
TCCGAAA	3760	0.0	37.75034	3
>>END_MODULE