FastQCFastQC Report
Thu 26 May 2016
SRR2048278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18516247
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC9602095.185764696269174TruSeq Adapter, Index 7 (97% over 35bp)
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT451950.24408293970154968No Hit
GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG356270.19240940132198495No Hit
GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC350830.1894714409458893No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT330980.1787511259706138No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT301730.16295418828664363No Hit
CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC285160.1540052905969552No Hit
GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC261930.14145955171153204No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC237900.1284817598296242No Hit
CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA235840.12736922336367623No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC222040.11991630917431594No Hit
CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA209590.11319248441652349No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT200220.10813206369519698No Hit
CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA200110.10807265640818034No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG199480.10773241467344867No Hit
CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA198010.10693851729240812No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG190830.10306084164895835No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG190140.10268819593949033No Hit
CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC188350.10172147735985591No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA187810.10142984158722876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT103050.045.9569131
CTTGAAA1184100.041.33679660-61
GCTTGAA1203350.041.26157458-59
CTGCTTG1229050.040.6461156-57
TGCTTGA1211650.040.4928358-59
TTGAAAA1230750.040.29677260-61
TGAAAAA1218600.040.1275362-63
GCCGTCT1246200.039.77992648-49
ATGCCGT1260200.039.39047646-47
CGTCTTC1259400.039.33469850-51
GGCGATC118600.038.897112
GTATGCC1279400.038.86245744-45
CCGTCTT1268400.038.6380650-51
TCGTATG1292800.038.39534842-43
TCTTCTG1313950.037.69444752-53
TGCCGTC1319850.037.14087748-49
CGTATGC1328400.036.9498144-45
TATCTCG1334300.036.48768638-39
CTTCTGC1354850.036.4040354-55
GTCTTCT1349300.036.2457552-53
Produced by FastQC (version 0.11.5)