##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048278_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18516247 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.085218835112755 33.0 31.0 34.0 30.0 34.0 2 32.18091738568836 34.0 31.0 34.0 30.0 34.0 3 32.28685202784344 34.0 31.0 34.0 30.0 34.0 4 35.90034033354599 37.0 35.0 37.0 35.0 37.0 5 35.72115007971108 37.0 35.0 37.0 33.0 37.0 6 35.67263209439796 37.0 35.0 37.0 33.0 37.0 7 35.61702395739266 37.0 35.0 37.0 33.0 37.0 8 35.684334087787875 37.0 35.0 37.0 33.0 37.0 9 37.291044616114704 39.0 37.0 39.0 34.0 39.0 10-11 37.330621966751686 39.0 37.0 39.0 34.0 39.0 12-13 37.344161616552206 39.0 37.0 39.0 34.0 39.0 14-15 38.654052006327206 40.0 38.0 41.0 34.0 41.0 16-17 38.6171128793 40.0 38.0 41.0 34.0 41.0 18-19 38.56653583741889 40.0 38.0 41.0 34.0 41.0 20-21 38.55763635579068 40.0 38.0 41.0 34.0 41.0 22-23 38.51337811598646 40.0 38.0 41.0 34.0 41.0 24-25 38.42745084897604 40.0 38.0 41.0 34.0 41.0 26-27 38.3202851258141 40.0 38.0 41.0 33.5 41.0 28-29 38.20608436472034 40.0 38.0 41.0 33.0 41.0 30-31 38.03793022959782 40.0 37.0 41.0 33.0 41.0 32-33 38.00226868328123 40.0 37.0 41.0 33.0 41.0 34-35 37.89133043537386 40.0 37.0 41.0 33.0 41.0 36-37 37.71588259759119 40.0 37.0 41.0 32.5 41.0 38-39 37.474223232170104 40.0 36.5 41.0 31.5 41.0 40-41 37.21886886689295 39.5 35.5 41.0 31.0 41.0 42-43 36.98486040394687 39.0 35.0 41.0 31.0 41.0 44-45 36.98869036473752 39.0 35.0 41.0 31.0 41.0 46-47 36.70857018163562 39.0 35.0 41.0 30.5 41.0 48-49 36.6986086327321 39.0 35.0 41.0 31.0 41.0 50-51 36.6552147959573 39.0 35.0 41.0 31.0 41.0 52-53 36.330661985660484 38.5 35.0 41.0 30.5 41.0 54-55 36.09824731221181 38.0 35.0 40.0 30.0 41.0 56-57 35.87016958674185 37.5 35.0 40.0 30.0 41.0 58-59 35.54412767878934 37.0 34.0 40.0 29.5 41.0 60-61 35.271540555707645 36.0 34.0 39.0 29.0 41.0 62-63 34.978974653989006 36.0 34.0 39.0 29.0 41.0 64-65 34.47527773851796 35.0 34.0 38.5 28.5 41.0 66-67 34.24920295673308 35.0 34.0 37.5 29.0 40.0 68-69 33.81213544515798 35.0 33.0 37.0 28.5 39.5 70-71 33.26495587901803 35.0 33.0 36.5 26.5 39.0 72-73 32.4205094585312 35.0 33.0 36.0 25.0 39.0 74-75 31.65395776476734 35.0 33.0 35.5 21.5 37.5 76-77 31.528676896565486 35.0 33.0 35.0 21.5 37.0 78-79 31.41894461658456 35.0 33.0 35.0 22.5 37.0 80-81 31.291573854032084 35.0 33.0 35.0 22.5 36.0 82-83 31.14415251103531 35.0 33.0 35.0 21.0 36.0 84-85 31.02848768435634 35.0 32.5 35.0 20.0 36.0 86-87 30.915707270485214 35.0 32.0 35.0 20.0 35.0 88-89 30.820459945257802 35.0 32.0 35.0 20.0 35.0 90-91 30.7310014821038 35.0 32.0 35.0 19.5 35.0 92-93 30.618683742985283 35.0 32.0 35.0 18.5 35.0 94-95 30.526159296751658 35.0 32.0 35.0 17.5 35.0 96-97 30.411503718869163 35.0 32.0 35.0 11.5 35.0 98-99 30.247227070366904 35.0 32.0 35.0 2.0 35.0 100-101 29.679641587196368 34.5 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4637199876425768 1101 2 0.4778778527969436 1101 3 0.4419225276368337 1101 4 0.3100113923559036 1101 5 0.38118132995400344 1101 6 0.3416373036328366 1101 7 0.3969267324518455 1101 8 0.3908556617184189 1101 9 0.48424018047246875 1101 10-11 0.43801272443265304 1101 12-13 0.436158157080925 1101 14-15 0.5925782697001338 1101 16-17 0.6094070363616098 1101 18-19 0.5941978846483806 1101 20-21 0.5977967786228149 1101 22-23 0.6064061857648753 1101 24-25 0.5781175978102624 1101 26-27 0.5713111029643585 1101 28-29 0.6139846446298876 1101 30-31 0.6183120461079596 1101 32-33 0.5847116040569844 1101 34-35 0.6473457133536087 1101 36-37 0.6985722420605356 1101 38-39 0.7065037634060332 1101 40-41 0.6375915601580218 1101 42-43 0.7527447744879083 1101 44-45 0.651103259374473 1101 46-47 0.6830530732714166 1101 48-49 0.7032449079967193 1101 50-51 0.6650182783228118 1101 52-53 0.6448190101311511 1101 54-55 0.6533645444395191 1101 56-57 0.6824130381326512 1101 58-59 0.6168324028979555 1101 60-61 0.5835124910648588 1101 62-63 0.5280636065851141 1101 64-65 0.5527856046053685 1101 66-67 0.5367641855256906 1101 68-69 0.5357097143612677 1101 70-71 0.5420124559820181 1101 72-73 0.5177790984299406 1101 74-75 0.4780294047508491 1101 76-77 0.4314292244689817 1101 78-79 0.41317371868459674 1101 80-81 0.43361107745285565 1101 82-83 0.42398428591500803 1101 84-85 0.4318130792124961 1101 86-87 0.3984187162017534 1101 88-89 0.440563558935807 1101 90-91 0.44269633308671175 1101 92-93 0.4350880313237262 1101 94-95 0.4437395737072549 1101 96-97 0.4668021373347919 1101 98-99 0.487110105445808 1101 100-101 0.5665760662269292 1102 1 0.4269027979583484 1102 2 0.44424789242521 1102 3 0.41488574395896904 1102 4 0.29803655843192445 1102 5 0.3487097886931281 1102 6 0.3479393156378734 1102 7 0.3945516548746397 1102 8 0.3741580121980945 1102 9 0.46246662592621846 1102 10-11 0.4140235778778063 1102 12-13 0.4007979941664246 1102 14-15 0.5366790752778599 1102 16-17 0.580834599013663 1102 18-19 0.5386879556348703 1102 20-21 0.5464808156295717 1102 22-23 0.5339049516581866 1102 24-25 0.5448674238502846 1102 26-27 0.5471344084852632 1102 28-29 0.6101034133112577 1102 30-31 0.5842271556506802 1102 32-33 0.5825438281973803 1102 34-35 0.6203284420017567 1102 36-37 0.6817850113656547 1102 38-39 0.6610393827700989 1102 40-41 0.6511586104738782 1102 42-43 0.6544026481761733 1102 44-45 0.5963331662376774 1102 46-47 0.690186073068773 1102 48-49 0.6414566957571495 1102 50-51 0.5969785472255964 1102 52-53 0.5847514364023354 1102 54-55 0.612697585588279 1102 56-57 0.6369773423728091 1102 58-59 0.6503549897225298 1102 60-61 0.6479344509654439 1102 62-63 0.5751817545205284 1102 64-65 0.6081674732999076 1102 66-67 0.5388524608705367 1102 68-69 0.5473826852294579 1102 70-71 0.5708839277683211 1102 72-73 0.5119709817984059 1102 74-75 0.4631405943002491 1102 76-77 0.4335045568434843 1102 78-79 0.4504133005296467 1102 80-81 0.44090475010009555 1102 82-83 0.4752338558183524 1102 84-85 0.47762059697293324 1102 86-87 0.4906399687182308 1102 88-89 0.47291064825013507 1102 90-91 0.4799292396834538 1102 92-93 0.4890366918758282 1102 94-95 0.466487031820666 1102 96-97 0.4913600237099658 1102 98-99 0.5164683168292825 1102 100-101 0.6205006747065696 1103 1 0.3391498295690667 1103 2 0.3606167924049686 1103 3 0.3046555127887487 1103 4 0.22728306780260965 1103 5 0.24224072435704613 1103 6 0.26968993912927175 1103 7 0.2987977378440547 1103 8 0.2882472079256502 1103 9 0.34039526473043225 1103 10-11 0.31901164679438665 1103 12-13 0.34933904523835224 1103 14-15 0.4743617315518378 1103 16-17 0.48744319202911157 1103 18-19 0.474454920847478 1103 20-21 0.49750241671198836 1103 22-23 0.4917962303162611 1103 24-25 0.4870800201184835 1103 26-27 0.4474489391266232 1103 28-29 0.48765708644274497 1103 30-31 0.42011785890645115 1103 32-33 0.4960288327162061 1103 34-35 0.4701372881595418 1103 36-37 0.5432298967190476 1103 38-39 0.545001851887676 1103 40-41 0.4858788414765769 1103 42-43 0.5324379645782855 1103 44-45 0.5508326659692742 1103 46-47 0.6131792842695489 1103 48-49 0.5204113993711132 1103 50-51 0.49533609982799476 1103 52-53 0.46956549205025055 1103 54-55 0.4535859337573882 1103 56-57 0.5079229451107352 1103 58-59 0.487589420675576 1103 60-61 0.4830066645516169 1103 62-63 0.44392697591692354 1103 64-65 0.44306112112467844 1103 66-67 0.4281126074449304 1103 68-69 0.45833347515252143 1103 70-71 0.45348431908407605 1103 72-73 0.39752419208298306 1103 74-75 0.3871834723940495 1103 76-77 0.39530250457263705 1103 78-79 0.3746385045652332 1103 80-81 0.36754952073306413 1103 82-83 0.352020577162655 1103 84-85 0.38672844846838217 1103 86-87 0.3992823010935602 1103 88-89 0.3911009893054498 1103 90-91 0.37902794785670224 1103 92-93 0.3836493328667565 1103 94-95 0.41944234904030253 1103 96-97 0.42987140540549973 1103 98-99 0.49212417487120064 1103 100-101 0.5188671977977357 1104 1 0.23005814731568108 1104 2 0.24803216492111346 1104 3 0.2533813469046464 1104 4 0.1860937040253674 1104 5 0.20676703034312283 1104 6 0.2618943153112099 1104 7 0.2581031392570736 1104 8 0.2373840528630069 1104 9 0.28950559284059807 1104 10-11 0.29725426670864863 1104 12-13 0.28161894910515173 1104 14-15 0.4114987420576739 1104 16-17 0.37694555202673286 1104 18-19 0.342160125518582 1104 20-21 0.320574869738401 1104 22-23 0.29377575997241934 1104 24-25 0.3293542131661553 1104 26-27 0.3161164297551906 1104 28-29 0.29196306346494794 1104 30-31 0.3141060091184613 1104 32-33 0.35673278656916096 1104 34-35 0.3689145552189146 1104 36-37 0.3649988213258908 1104 38-39 0.4107063296096598 1104 40-41 0.3254992498615863 1104 42-43 0.4461404865225731 1104 44-45 0.3883328599948399 1104 46-47 0.450918210583815 1104 48-49 0.39922990333052155 1104 50-51 0.34956930109459705 1104 52-53 0.35529542916010115 1104 54-55 0.3841662545204514 1104 56-57 0.3294874961226526 1104 58-59 0.3650102938164892 1104 60-61 0.3662847652741661 1104 62-63 0.35112252716942294 1104 64-65 0.320922270609735 1104 66-67 0.29424749854484844 1104 68-69 0.33665551619798606 1104 70-71 0.296320539517815 1104 72-73 0.255036910458756 1104 74-75 0.2541771691502035 1104 76-77 0.21681232420647234 1104 78-79 0.22908408298131278 1104 80-81 0.20419042403848664 1104 82-83 0.2312583380300275 1104 84-85 0.2455624000965173 1104 86-87 0.23588827979731164 1104 88-89 0.25744162834805095 1104 90-91 0.24580457851222093 1104 92-93 0.27810928857718764 1104 94-95 0.28163084099600155 1104 96-97 0.28809716773194083 1104 98-99 0.32897009852343473 1104 100-101 0.37379234273483064 1105 1 0.25189474492280084 1105 2 0.257799057022261 1105 3 0.27552187332693023 1105 4 0.17676724870621996 1105 5 0.1905188303558134 1105 6 0.18459851864640342 1105 7 0.19538986298353933 1105 8 0.18675938324121688 1105 9 0.2527753403726649 1105 10-11 0.22216115259343638 1105 12-13 0.21794909135029172 1105 14-15 0.287681374418284 1105 16-17 0.2603731723533045 1105 18-19 0.2430468772043639 1105 20-21 0.3211801821568159 1105 22-23 0.31618265495616527 1105 24-25 0.28634440836080444 1105 26-27 0.2842224356640912 1105 28-29 0.2923291127883161 1105 30-31 0.29029672734679934 1105 32-33 0.2543135437985242 1105 34-35 0.25959180592247577 1105 36-37 0.2448527401443954 1105 38-39 0.29558583936173477 1105 40-41 0.3206086855399164 1105 42-43 0.3546556488228134 1105 44-45 0.2680274131792473 1105 46-47 0.3516575279230878 1105 48-49 0.29259477573681636 1105 50-51 0.25260536840882963 1105 52-53 0.266640149297082 1105 54-55 0.30373359793107824 1105 56-57 0.3115301273982709 1105 58-59 0.2663568723604044 1105 60-61 0.25933368950089886 1105 62-63 0.2592280747182656 1105 64-65 0.2538227571632561 1105 66-67 0.24077019496080965 1105 68-69 0.24064493458563163 1105 70-71 0.20696139760270427 1105 72-73 0.19008129210226343 1105 74-75 0.1721073268546931 1105 76-77 0.19080813491807547 1105 78-79 0.19023377580599998 1105 80-81 0.1569769812484445 1105 82-83 0.16246810759245278 1105 84-85 0.163818166689488 1105 86-87 0.15553314675599594 1105 88-89 0.13930066371102612 1105 90-91 0.11764214649004501 1105 92-93 0.12360304584234783 1105 94-95 0.09781776282233778 1105 96-97 0.07150202734509747 1105 98-99 0.09910564155675416 1105 100-101 0.15731731068929733 1106 1 0.17138157224115247 1106 2 0.1973514910092078 1106 3 0.1457914033036758 1106 4 0.10005270749775264 1106 5 0.11212249148249498 1106 6 0.12884940470746642 1106 7 0.17125821983163547 1106 8 0.16219187079700959 1106 9 0.22815899534056427 1106 10-11 0.1892167134846119 1106 12-13 0.13778691142097443 1106 14-15 0.21035921671107616 1106 16-17 0.1622546126102904 1106 18-19 0.20024616230810466 1106 20-21 0.19625209455667658 1106 22-23 0.18891958630950967 1106 24-25 0.20409436012161564 1106 26-27 0.20794656188290617 1106 28-29 0.19594810081751035 1106 30-31 0.16442912933965914 1106 32-33 0.14769792699943451 1106 34-35 0.1998400476618727 1106 36-37 0.18017617890336624 1106 38-39 0.2648985527481642 1106 40-41 0.14929320449930827 1106 42-43 0.2707268083475327 1106 44-45 0.26911246369235897 1106 46-47 0.33065882768293875 1106 48-49 0.27584024025871656 1106 50-51 0.20637979127921824 1106 52-53 0.2301144683044143 1106 54-55 0.26559710911672596 1106 56-57 0.21918825206508075 1106 58-59 0.20294667084429818 1106 60-61 0.2615362416076863 1106 62-63 0.21694446840996306 1106 64-65 0.18278655039083702 1106 66-67 0.2274373540285879 1106 68-69 0.17843150102535077 1106 70-71 0.15107986532052564 1106 72-73 0.13671231349201207 1106 74-75 0.13181601582446945 1106 76-77 0.14884941691524745 1106 78-79 0.12609601747416832 1106 80-81 0.12188752233693734 1106 82-83 0.09795958942321903 1106 84-85 0.12187230752418898 1106 86-87 0.07630188348233702 1106 88-89 0.10165801227361371 1106 90-91 0.16824865592076677 1106 92-93 0.17948540266370472 1106 94-95 0.20079925530464493 1106 96-97 0.1586681265283616 1106 98-99 0.19259101166734283 1106 100-101 0.21743172181210468 1107 1 0.04635059256381879 1107 2 0.09593101768506784 1107 3 0.1272864909797633 1107 4 0.09632173233704577 1107 5 -0.04505060914905812 1107 6 0.07395349889590364 1107 7 0.08885267992766188 1107 8 0.07901023691790243 1107 9 0.16267300054489908 1107 10-11 0.10074015359244015 1107 12-13 0.11928047174848899 1107 14-15 0.15620460811369696 1107 16-17 0.11002289465850623 1107 18-19 0.08962850584126159 1107 20-21 0.15326539434747843 1107 22-23 0.13865887177123426 1107 24-25 0.16110216094241991 1107 26-27 0.15760612902129623 1107 28-29 0.11488316086142447 1107 30-31 0.08698839437931838 1107 32-33 0.07931481467162627 1107 34-35 0.17981813306877825 1107 36-37 0.17804052061685383 1107 38-39 0.22218536383721244 1107 40-41 0.2199823968221395 1107 42-43 0.2356898750500065 1107 44-45 0.21500458990086457 1107 46-47 0.22420581034684517 1107 48-49 0.19852094625167638 1107 50-51 0.07594458357959155 1107 52-53 0.13849981236407416 1107 54-55 0.15365645713288245 1107 56-57 0.16981605517469234 1107 58-59 0.17363502557225274 1107 60-61 0.1802449939238997 1107 62-63 0.12720348540203474 1107 64-65 0.13698822295960866 1107 66-67 0.05360125391231918 1107 68-69 0.0820636461731894 1107 70-71 0.00913848402129247 1107 72-73 0.0471500251160748 1107 74-75 0.10707291224886717 1107 76-77 0.14031727688570328 1107 78-79 0.15884218804812633 1107 80-81 0.13869102758800622 1107 82-83 0.12493202694750138 1107 84-85 0.11444175155653369 1107 86-87 0.10932674752204363 1107 88-89 0.12974695190859364 1107 90-91 0.11753506063721275 1107 92-93 0.10159095760523229 1107 94-95 0.07195434457639038 1107 96-97 0.09161244237674993 1107 98-99 0.11114736653360069 1107 100-101 0.13868226032482056 1108 1 0.11843260904767305 1108 2 0.13816401347689578 1108 3 0.12714789836204687 1108 4 0.062142421040540796 1108 5 -0.0050421426613951326 1108 6 0.08272005095114565 1108 7 0.1307583599650286 1108 8 0.10283608872546068 1108 9 0.14084430389527114 1108 10-11 0.11301521310221574 1108 12-13 0.14052216617361069 1108 14-15 0.1561862608628033 1108 16-17 0.10805236198051915 1108 18-19 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-0.42417779780838316 2215 14-15 -0.5453248078439259 2215 16-17 -0.5688490715824841 2215 18-19 -0.6210769056545118 2215 20-21 -0.563070713262185 2215 22-23 -0.5317737327596461 2215 24-25 -0.5831634280269995 2215 26-27 -0.5827340027583858 2215 28-29 -0.5961375377284881 2215 30-31 -0.5483687163216189 2215 32-33 -0.6129908515548479 2215 34-35 -0.6562004552703797 2215 36-37 -0.5618781862715068 2215 38-39 -0.6241940867597862 2215 40-41 -0.64302207815998 2215 42-43 -0.6012041547223532 2215 44-45 -0.7273586001980235 2215 46-47 -0.6887718253719797 2215 48-49 -0.625184641567273 2215 50-51 -0.6285760737431971 2215 52-53 -0.6811039950910143 2215 54-55 -0.6365420221110085 2215 56-57 -0.6641566841371116 2215 58-59 -0.684136496484399 2215 60-61 -0.6196117122341533 2215 62-63 -0.6077053298813055 2215 64-65 -0.6633626726620676 2215 66-67 -0.6557824371944889 2215 68-69 -0.6726995805659115 2215 70-71 -0.7162070656318136 2215 72-73 -0.6415642287993002 2215 74-75 -0.4380306494792485 2215 76-77 -0.4421286569268261 2215 78-79 -0.48431792652441885 2215 80-81 -0.45589205251523524 2215 82-83 -0.42009673811499937 2215 84-85 -0.4568807379015922 2215 86-87 -0.5297432321445221 2215 88-89 -0.5459909793328706 2215 90-91 -0.4946636111865921 2215 92-93 -0.5738258247344383 2215 94-95 -0.5610342832041368 2215 96-97 -0.6316157441956776 2215 98-99 -0.623950042641205 2215 100-101 -0.7354874732396084 2216 1 -0.8950993457865906 2216 2 -0.7877025649294893 2216 3 -1.9450220074845923 2216 4 -0.8227430167748224 2216 5 -0.6810799154553351 2216 6 -0.7258313152930995 2216 7 -0.7546419595220684 2216 8 -0.6794188266540075 2216 9 -0.8882991806188372 2216 10-11 -0.8495858848440321 2216 12-13 -0.7090792962862835 2216 14-15 -0.9483969128611704 2216 16-17 -0.8485122667699443 2216 18-19 -0.8669340785606323 2216 20-21 -1.1347289023061222 2216 22-23 -0.8298936045951137 2216 24-25 -0.8420130177142795 2216 26-27 -0.774805116955676 2216 28-29 -0.8573349941807962 2216 30-31 -0.8065948638957394 2216 32-33 -0.98808387865909 2216 34-35 -1.012534232374982 2216 36-37 -1.0053465638221084 2216 38-39 -1.03264654618701 2216 40-41 -0.9742619666218033 2216 42-43 -1.0685795376528588 2216 44-45 -1.0356066453840285 2216 46-47 -0.7908490585477992 2216 48-49 -0.8949781681506437 2216 50-51 -1.152612181851282 2216 52-53 -1.2348311621454684 2216 54-55 -1.162069489782553 2216 56-57 -1.1981555326236872 2216 58-59 -1.2056195171408817 2216 60-61 -0.9615600647818567 2216 62-63 -0.926753663141028 2216 64-65 -1.04281493593367 2216 66-67 -1.0440042903577762 2216 68-69 -0.8880984948927306 2216 70-71 -0.9911258651022337 2216 72-73 -0.8616522059117848 2216 74-75 -0.6153754655699224 2216 76-77 -0.5908547555900938 2216 78-79 -0.39486920017679594 2216 80-81 -0.7388838717287243 2216 82-83 -0.5470217645585436 2216 84-85 -0.6021070305167484 2216 86-87 -0.5700433551292221 2216 88-89 -0.4738346911651945 2216 90-91 -0.5084121300430944 2216 92-93 -0.47246895750326345 2216 94-95 -0.6001381511256838 2216 96-97 -0.7560719355827636 2216 98-99 -0.6241339273687387 2216 100-101 -0.7561332216668788 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 396.0 3 493.0 4 904.0 5 1521.0 6 2840.0 7 5610.0 8 9688.0 9 12590.0 10 15922.0 11 21130.0 12 25581.0 13 26782.0 14 28377.0 15 30148.0 16 33412.0 17 37756.0 18 44619.0 19 54579.0 20 65310.0 21 77514.0 22 93730.0 23 117046.0 24 148576.0 25 194554.0 26 257543.0 27 365009.0 28 541051.0 29 311139.0 30 383571.0 31 472839.0 32 592831.0 33 762877.0 34 1048371.0 35 1596550.0 36 2806706.0 37 4458829.0 38 3401827.0 39 467456.0 40 570.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 47.185125768078215 15.773223961013407 7.840489451221358 29.201160819687015 2 30.95336681330403 21.413940923252557 22.10973831156422 25.522953951879195 3 30.408808980074376 15.117661084769177 23.322097168924024 31.151432766232425 4 33.16947003353326 19.015629895194206 17.191539948673185 30.623360122599358 5 37.99117359284848 20.768694166590873 19.151495289205474 22.088636951355166 6 33.351395386793484 26.057926317839502 20.205324123756373 20.385354171610643 7 23.02574652155449 27.074773379916945 29.827192616849292 20.07228748167928 8 22.62187364426495 26.132957720859956 27.598411276323976 23.646757358551117 9 26.941550304443446 18.958096638049817 30.77648510521598 23.323867952290765 10-11 27.224998672787205 27.528324179300483 23.210478343694593 22.036198804217726 12-13 24.089198884622395 25.813814634779668 23.337888163636674 26.759098316961268 14-15 24.296656876525788 26.10263030083796 24.02067492402753 25.580037898608722 16-17 27.660383878007245 22.942475869975162 23.369727677536382 26.02741257448121 18-19 24.92581245000674 22.60665727779501 26.31490333867333 26.15262693352492 20-21 27.814996743130504 22.73187433716995 25.742700451122737 23.710428468576815 22-23 24.95326131693966 28.89347123096813 22.100415381151485 24.052852070940727 24-25 24.352313403466695 22.66619958137305 25.992591803295777 26.988895211864477 26-27 24.64458566279063 22.329587640352766 22.72681487301718 30.29901182383942 28-29 27.100429692906992 25.313312681560145 22.321515801771277 25.26474182376159 30-31 24.27142582017488 23.155262025744545 25.283359456355974 27.2899526977246 32-33 23.741902752369533 25.429877239421998 23.28671868171115 27.54150132649732 34-35 24.47050150065507 25.519183234053855 22.63740054882612 27.37291471646495 36-37 27.44680286290947 22.186462842251338 22.79269149118271 27.574042803656486 38-39 24.41465865085943 25.37876601019634 25.236814998201307 24.969760340742916 40-41 24.83246008319559 25.735824901924758 25.259965955720403 24.171749059159254 42-43 24.694041683365022 22.79180214714877 25.541798386896208 26.972357782590002 44-45 28.513591334140227 22.70102575322094 22.249822007667106 26.535560904971728 46-47 24.984570751210192 25.501670328415692 25.241446309360143 24.27231261101397 48-49 27.48276149048995 23.05304363243804 25.479528869970245 23.98466600710176 50-51 23.937992491618775 23.401747055416187 22.895002746642724 29.765257706322313 52-53 27.082188955461657 23.53283848503425 25.26191727729707 24.123055282207027 54-55 24.758937380777002 22.887218992056006 25.25739422249012 27.096449404676875 56-57 23.624587639168997 23.0110642831671 25.822622154478715 27.54172592318519 58-59 26.64458947863463 23.26383688876045 25.554122819813323 24.537450812791597 60-61 24.023915321501164 23.399879576028553 25.519010412855263 27.057194689615017 62-63 26.95207349523907 23.566471110479352 25.214429252320947 24.267026141960628 64-65 25.103847988201927 28.12220532594969 22.59649323105271 24.17745345479567 66-67 24.839443570177792 28.15275861695573 22.1820719945965 24.825725818269976 68-69 24.631732877618234 28.0688818851898 22.482382634018656 24.81700260317331 70-71 24.307675848077103 28.56417699934036 22.594568901947497 24.53357825063504 72-73 24.675194708733365 28.797355641237665 22.07630682394764 24.45114282608133 74-75 25.277766946446263 28.024536512322534 22.123914237024987 24.573782304206222 76-77 25.708127104436656 26.50209778033745 22.74234917481618 25.047425940409713 78-79 25.03157632321496 26.02610021350439 23.439971393771103 25.50235206950955 80-81 25.702844950320923 25.826034067596638 23.470837447288183 25.000283534794253 82-83 25.909321837231193 25.410145143798218 23.50009767128646 25.180435347684128 84-85 25.669766693424418 25.8188529185404 22.43985588673616 26.071524501299017 86-87 26.061068574074763 25.897543198450713 22.17462907186138 25.866759155613146 88-89 26.05272691510428 25.253534769934948 22.117939654782262 26.575798660178513 90-91 25.720570392445897 25.034253725676614 22.921040531244095 26.324135350633394 92-93 25.50243231224501 25.598659244379736 22.791614185386294 26.107294257988954 94-95 25.600192220938723 26.38973356996225 22.45257704972075 25.55749715937828 96-97 25.624746065901594 26.146188918706148 22.54175185593658 25.687313159455684 98-99 24.934176020685843 26.4321071474641 22.61186579552861 26.021851036321443 100-101 24.75168011556622 26.78957819333482 22.26204094298935 26.19670074810961 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 348.0 1 249.0 2 134.0 3 110.0 4 91.0 5 85.5 6 91.0 7 98.5 8 113.5 9 138.0 10 159.5 11 183.0 12 253.0 13 339.5 14 406.5 15 618.0 16 993.5 17 1732.0 18 2269.5 19 2664.5 20 3423.5 21 4548.5 22 5874.5 23 7018.5 24 8673.5 25 10777.5 26 13819.0 27 17528.5 28 20464.0 29 26023.0 30 38106.5 31 43314.0 32 43044.5 33 55182.5 34 68286.5 35 88654.0 36 121493.0 37 143211.0 38 179937.0 39 244633.5 40 328242.5 41 424917.5 42 520604.5 43 600430.0 44 688284.5 45 781995.5 46 877204.0 47 934742.0 48 934466.0 49 912753.0 50 859341.5 51 791115.5 52 732585.0 53 692187.5 54 624002.0 55 576387.0 56 568063.0 57 527629.0 58 485082.5 59 482797.5 60 461499.0 61 419963.0 62 414388.5 63 430878.0 64 402647.0 65 302332.5 66 212472.0 67 185219.0 68 189121.0 69 150527.5 70 96346.5 71 73478.0 72 60790.0 73 52384.5 74 48724.0 75 57735.0 76 61751.0 77 58176.5 78 55691.5 79 43638.0 80 32543.5 81 30613.0 82 29469.5 83 25870.0 84 21633.5 85 21002.0 86 23873.0 87 21966.0 88 13920.0 89 7586.5 90 4754.0 91 2828.5 92 1585.0 93 1094.5 94 759.0 95 474.0 96 337.5 97 239.5 98 119.0 99 51.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08385608595521545 2 0.011303586520529782 3 7.668940687602622E-4 4 0.0 5 0.004855195547996309 6 0.002424897442770125 7 0.005443867755706651 8 0.0 9 0.0 10-11 0.0 12-13 3.5104305964378204E-5 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0055410796799156975 28-29 0.0 30-31 7.020861192875641E-5 32-33 8.100993684087276E-6 34-35 0.0 36-37 2.61932129118822E-4 38-39 0.0 40-41 0.0019496391466370046 42-43 0.0010990348098078406 44-45 0.0 46-47 0.0014986838315561462 48-49 0.0 50-51 2.970364350832002E-5 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.049619396414402E-4 68-69 0.0 70-71 3.5104305964378204E-4 72-73 0.0 74-75 2.7003312280290923E-5 76-77 4.860596210452367E-5 78-79 0.0 80-81 5.400662456058185E-6 82-83 3.1323842245137474E-4 84-85 6.291771761307786E-4 86-87 8.668063241973387E-4 88-89 2.646324603468511E-4 90-91 7.020861192875641E-5 92-93 6.534801571830404E-4 94-95 2.91635772627142E-4 96-97 6.69682144551215E-4 98-99 4.5905630876494574E-5 100-101 5.940728701664004E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.8516247E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.55509694744932 #Duplication Level Percentage of deduplicated Percentage of total 1 84.39714416230974 30.007486427787793 2 10.231279552264288 7.275482727544253 3 2.341260475092465 2.4973122951343156 4 0.8909505581043758 1.2671133387514066 5 0.45965614043223757 0.8171559317779292 6 0.29976571964292753 0.6394919534055721 7 0.18919196014908 0.4708716939344955 8 0.1327987386732935 0.37773376224223504 9 0.0979808493556794 0.31353467380511474 >10 0.6864936364403736 4.884997554320162 >50 0.10136773954583639 2.5630665795897447 >100 0.12015635908484087 9.394131475059195 >500 0.02481588178627309 6.189150418506253 >1k 0.023881046198726687 17.18841804011892 >5k 0.0023743935680250144 5.675709647095636 >10k+ 8.827873522144284E-4 10.438343480927118 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC 960209 5.185764696269174 TruSeq Adapter, Index 7 (97% over 35bp) GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT 45195 0.24408293970154968 No Hit GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG 35627 0.19240940132198495 No Hit GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC 35083 0.1894714409458893 No Hit CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT 33098 0.1787511259706138 No Hit GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT 30173 0.16295418828664363 No Hit CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC 28516 0.1540052905969552 No Hit GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC 26193 0.14145955171153204 No Hit GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC 23790 0.1284817598296242 No Hit CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA 23584 0.12736922336367623 No Hit GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC 22204 0.11991630917431594 No Hit CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA 20959 0.11319248441652349 No Hit CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT 20022 0.10813206369519698 No Hit CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA 20011 0.10807265640818034 No Hit GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG 19948 0.10773241467344867 No Hit CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA 19801 0.10693851729240812 No Hit GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATG 19083 0.10306084164895835 No Hit GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG 19014 0.10268819593949033 No Hit CACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCC 18835 0.10172147735985591 No Hit CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA 18781 0.10142984158722876 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7282119859386193E-4 0.0 0.0 5.400662456058185E-6 5.400662456058185E-6 2 2.484304729786765E-4 3.240397473634911E-5 0.0 5.400662456058185E-6 5.400662456058185E-6 3 2.646324603468511E-4 5.940728701664004E-5 0.0 1.080132491211637E-5 5.400662456058185E-6 4 3.0243709753925837E-4 6.480794947269822E-5 0.0 1.6201987368174556E-5 5.400662456058185E-6 5 3.942483592922475E-4 7.020861192875641E-5 0.0 1.6201987368174556E-5 5.400662456058185E-6 6 4.806589585891785E-4 7.560927438481459E-5 0.0 1.6201987368174556E-5 5.400662456058185E-6 7 7.614934063042041E-4 8.100993684087278E-5 0.0 2.160264982423274E-5 1.080132491211637E-5 8 0.001080132491211637 9.181126175298915E-5 0.0 2.160264982423274E-5 1.080132491211637E-5 9 0.0012475530273494408 9.721192420904733E-5 0.0 2.7003312280290926E-5 1.080132491211637E-5 10-11 0.0020009454399695574 9.721192420904733E-5 0.0 3.240397473634911E-5 1.080132491211637E-5 12-13 0.002757038183817703 9.991225543707643E-5 0.0 3.7804637192407297E-5 1.6201987368174556E-5 14-15 0.0031647881992500964 1.080132491211637E-4 0.0 3.7804637192407297E-5 1.6201987368174556E-5 16-17 0.0036562484827513913 1.107135803491928E-4 0.0 3.7804637192407297E-5 1.6201987368174556E-5 18-19 0.004118005122744366 1.1611424280525098E-4 5.400662456058185E-6 4.050496842043639E-5 2.160264982423274E-5 20-21 0.005457369411846796 1.2151490526130917E-4 5.400662456058185E-6 4.320529964846548E-5 2.160264982423274E-5 22-23 0.011314387845441897 1.2421523648933826E-4 5.400662456058185E-6 4.320529964846548E-5 2.160264982423274E-5 24-25 0.024888952928744143 1.3231623017342554E-4 1.080132491211637E-5 4.320529964846548E-5 2.7003312280290926E-5 26-27 0.04381017384354399 1.5661921122568737E-4 1.080132491211637E-5 4.320529964846548E-5 2.7003312280290926E-5 28-29 0.07438062367606135 1.6201987368174556E-4 1.080132491211637E-5 4.320529964846548E-5 2.7003312280290926E-5 30-31 0.11941674789713055 1.6201987368174556E-4 1.080132491211637E-5 4.320529964846548E-5 2.7003312280290926E-5 32-33 0.1729589154864914 1.6472020490977465E-4 1.080132491211637E-5 4.320529964846548E-5 2.7003312280290926E-5 34-35 0.23334102207644997 1.7552152982189102E-4 1.080132491211637E-5 5.130629333255276E-5 2.970364350832002E-5 36-37 0.3055235761328956 1.809221922779492E-4 1.080132491211637E-5 6.210761824466913E-5 3.240397473634911E-5 38-39 0.3891555345961847 1.836225235059783E-4 1.080132491211637E-5 7.29089431567855E-5 3.240397473634911E-5 40-41 0.48338089246703175 1.836225235059783E-4 1.080132491211637E-5 8.100993684087278E-5 3.240397473634911E-5 42-43 0.5869223930745793 1.9442384841809467E-4 1.080132491211637E-5 8.100993684087278E-5 3.240397473634911E-5 44-45 0.6954730081101208 2.2952815438247287E-4 1.080132491211637E-5 8.641059929693096E-5 3.240397473634911E-5 46-47 0.810690740947666 2.592317978907929E-4 1.080132491211637E-5 8.641059929693096E-5 3.240397473634911E-5 48-49 0.9435632393540656 2.592317978907929E-4 1.080132491211637E-5 8.641059929693096E-5 3.240397473634911E-5 50-51 1.0914279767384827 2.592317978907929E-4 1.080132491211637E-5 8.641059929693096E-5 3.240397473634911E-5 52-53 1.2545927908609125 2.646324603468511E-4 1.080132491211637E-5 9.181126175298915E-5 3.240397473634911E-5 54-55 1.4335248390238045 2.7543378525896745E-4 1.080132491211637E-5 9.721192420904733E-5 3.240397473634911E-5 56-57 1.6468186020633664 2.997367663112293E-4 1.080132491211637E-5 9.991225543707643E-5 3.240397473634911E-5 58-59 1.89314281668418 3.294404098195493E-4 1.6201987368174556E-5 1.1341391157722189E-4 3.240397473634911E-5 60-61 2.16321644445551 3.429420659596948E-4 1.6201987368174556E-5 1.1341391157722189E-4 3.240397473634911E-5 62-63 2.4772865689251176 4.050496842043639E-4 1.6201987368174556E-5 1.1611424280525098E-4 3.7804637192407297E-5 64-65 2.8397439286697783 4.563559775369166E-4 1.6201987368174556E-5 1.1881457403328007E-4 3.7804637192407297E-5 66-67 3.24585214271553 4.806589585891785E-4 1.6201987368174556E-5 1.1881457403328007E-4 3.7804637192407297E-5 68-69 3.7223552915447717 4.887599522732657E-4 1.6201987368174556E-5 1.2961589894539645E-4 3.7804637192407297E-5 70-71 4.2425362979873835 4.96860945957353E-4 1.6201987368174556E-5 1.3501656140145463E-4 4.860596210452367E-5 72-73 4.781749238925145 4.995612771853821E-4 1.6201987368174556E-5 1.431175550855419E-4 4.860596210452367E-5 74-75 5.360843371769668 5.076622708694694E-4 1.6201987368174556E-5 1.5391887999765828E-4 4.860596210452367E-5 76-77 5.996385228604911 5.157632645535566E-4 1.6201987368174556E-5 1.6742053613780374E-4 4.860596210452367E-5 78-79 6.696924058098814 5.184635957815858E-4 1.6201987368174556E-5 1.782218610499201E-4 4.860596210452367E-5 80-81 7.462773098673829 5.184635957815858E-4 1.6201987368174556E-5 1.9172351719006558E-4 5.130629333255276E-5 82-83 8.278443250405982 5.238642582376439E-4 1.6201987368174556E-5 1.9712417964612376E-4 5.400662456058185E-5 84-85 9.138898395554996 5.238642582376439E-4 1.6201987368174556E-5 2.0252484210218195E-4 5.400662456058185E-5 86-87 9.988379394593299 5.238642582376439E-4 1.6201987368174556E-5 2.0522517333021104E-4 5.940728701664004E-5 88-89 10.835781138586022 5.238642582376439E-4 1.6201987368174556E-5 2.160264982423274E-4 5.940728701664004E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGAT 10305 0.0 45.956913 1 CTTGAAA 118410 0.0 41.336796 60-61 GCTTGAA 120335 0.0 41.261574 58-59 CTGCTTG 122905 0.0 40.64611 56-57 TGCTTGA 121165 0.0 40.49283 58-59 TTGAAAA 123075 0.0 40.296772 60-61 TGAAAAA 121860 0.0 40.12753 62-63 GCCGTCT 124620 0.0 39.779926 48-49 ATGCCGT 126020 0.0 39.390476 46-47 CGTCTTC 125940 0.0 39.334698 50-51 GGCGATC 11860 0.0 38.89711 2 GTATGCC 127940 0.0 38.862457 44-45 CCGTCTT 126840 0.0 38.63806 50-51 TCGTATG 129280 0.0 38.395348 42-43 TCTTCTG 131395 0.0 37.694447 52-53 TGCCGTC 131985 0.0 37.140877 48-49 CGTATGC 132840 0.0 36.94981 44-45 TATCTCG 133430 0.0 36.487686 38-39 CTTCTGC 135485 0.0 36.40403 54-55 GTCTTCT 134930 0.0 36.24575 52-53 >>END_MODULE