FastQCFastQC Report
Thu 26 May 2016
SRR2048277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16765309
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC448530.26753458585225No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT434360.2590826092140622No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT432150.2577644110227852No Hit
CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC401040.23920823648403977No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT362030.21593995076380637No Hit
GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG290200.17309552719845486No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT281920.16815675750443967No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG263350.15708031387909402No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG253380.15113351027410232No Hit
CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA244810.14602176434684264No Hit
GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC239650.14294398033463027No Hit
CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA236730.14120228860678918No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG235660.14056406595309398No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA231750.13823186915314237No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT220640.13160509001056886No Hit
CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA215330.12843783553288518No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG213070.12708981385311777No Hit
CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA203580.12142931573763417No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC193750.11556601790041568No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA178330.10636845404996711No Hit
GTCCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGC174820.10427484515793893No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT174130.103863281016771No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG170740.10184124849711987No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA53450.047.695672
GGGCGAT78300.037.847981
GGCGATC79950.036.518172
GGCGCGT94750.034.0698625
CGGCATT166650.032.4193953
GCGATCT103600.029.2825623
GCGTGCC111300.029.0392198
GCATTCT198200.027.4965975
TAAAACG152450.027.14210958-59
ACGCTCT152600.026.86511862-63
CGAAATT157050.026.47948352-53
TCAACGA159900.026.11212348-49
GGCATTC213400.026.048644
TCGGGAA30850.025.5458813
AACGCAC160750.025.4835372-73
CGCGTGC128450.025.0511287
AACGAAA167450.024.89121250-51
CGTGCCT133700.024.635489
CCGGCAT217400.024.6321322
CGGTGGC140000.024.468931
Produced by FastQC (version 0.11.5)