##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048277_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16765309
Sequences flagged as poor quality	0
Sequence length	101
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.322368111437733	31.0	31.0	34.0	27.0	34.0
2	30.42730008734107	31.0	31.0	34.0	27.0	34.0
3	30.757621228454543	31.0	31.0	34.0	27.0	34.0
4	34.43398925722156	37.0	35.0	37.0	32.0	37.0
5	34.432627397443134	37.0	35.0	37.0	32.0	37.0
6	34.35227361452151	37.0	35.0	37.0	31.0	37.0
7	34.321711577162105	37.0	35.0	37.0	31.0	37.0
8	34.45904068931864	37.0	35.0	37.0	31.0	37.0
9	36.02047782119614	39.0	37.0	39.0	32.0	39.0
10-11	35.98295864991215	39.0	37.0	39.0	31.5	39.0
12-13	35.92511166957913	39.0	37.0	39.0	31.5	39.0
14-15	37.07235843371572	40.0	37.0	41.0	31.0	41.0
16-17	37.08156399026108	40.0	37.0	41.0	31.0	41.0
18-19	36.970683958166234	40.0	37.0	41.0	31.0	41.0
20-21	36.90976578481196	40.0	37.0	41.0	31.0	41.0
22-23	36.82398335157437	40.0	37.0	41.0	30.5	41.0
24-25	36.71763335826378	40.0	37.0	41.0	30.0	41.0
26-27	36.64980427739208	40.0	37.0	41.0	30.0	41.0
28-29	36.47825563489465	40.0	36.0	41.0	30.0	41.0
30-31	36.356330563307836	40.0	36.0	41.0	30.0	41.0
32-33	36.289314142674016	40.0	36.0	41.0	30.0	41.0
34-35	35.84430904315572	39.5	35.0	41.0	28.0	41.0
36-37	35.78849835096985	39.0	35.0	41.0	27.5	41.0
38-39	35.76729483482828	39.0	35.0	41.0	28.0	41.0
40-41	35.55775595904615	39.0	35.0	40.5	27.5	41.0
42-43	35.02200215933986	38.0	34.0	40.0	26.0	41.0
44-45	34.78094155616219	38.0	34.0	40.0	25.5	41.0
46-47	34.57692903244432	38.0	33.5	40.0	24.5	41.0
48-49	34.32596738896969	38.0	33.0	40.0	24.0	41.0
50-51	34.30313718047189	38.0	33.0	40.0	24.0	41.0
52-53	34.1799949824963	37.0	33.0	40.0	24.0	41.0
54-55	34.089199906783705	37.0	33.0	40.0	24.0	41.0
56-57	33.8143577908406	37.0	33.0	40.0	23.0	41.0
58-59	33.52626855848585	36.0	33.0	40.0	23.0	41.0
60-61	33.22500482991396	35.5	33.0	39.0	22.5	41.0
62-63	32.90420540414733	35.0	33.0	39.0	22.0	41.0
64-65	32.62023900066501	35.0	32.0	38.5	22.0	40.0
66-67	32.311910057846234	35.0	32.0	37.5	21.5	40.0
68-69	31.946934530106184	35.0	32.0	37.0	20.0	40.0
70-71	31.66067720553197	35.0	31.5	36.5	20.0	39.0
72-73	31.41430578464137	35.0	31.0	36.0	20.0	39.0
74-75	31.145540264125167	35.0	31.0	36.0	19.5	38.5
76-77	30.837119733373243	35.0	31.0	35.0	18.0	37.0
78-79	30.610259464946335	34.5	31.0	35.0	18.0	37.0
80-81	30.438630537617886	34.0	31.0	35.0	17.0	36.0
82-83	30.237907395563063	34.0	31.0	35.0	15.5	36.0
84-85	30.13665259614362	34.0	31.0	35.0	15.0	36.0
86-87	30.33013972483299	34.5	31.0	35.0	16.5	35.5
88-89	30.266809487376584	35.0	31.0	35.0	15.0	35.0
90-91	30.150653739814757	35.0	31.0	35.0	11.0	35.0
92-93	30.02796772191911	35.0	31.0	35.0	6.0	35.0
94-95	29.919108797815774	34.5	31.0	35.0	2.0	35.0
96-97	29.752332629240534	34.0	31.0	35.0	2.0	35.0
98-99	29.55859930765368	34.0	31.0	35.0	2.0	35.0
100-101	28.855083136254752	34.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6222492022541708
1101	2	0.5642924558334208
1101	3	0.6160435602224297
1101	4	0.6320966951711995
1101	5	0.6804423932983283
1101	6	0.6132652331257233
1101	7	0.6416779734298288
1101	8	0.6287011508615308
1101	9	0.7333414026833793
1101	10-11	0.7056843885644355
1101	12-13	0.7348208579314814
1101	14-15	0.8753944788289516
1101	16-17	0.8782623466316011
1101	18-19	0.8924085491116713
1101	20-21	0.8713319035801774
1101	22-23	0.8650541988046783
1101	24-25	0.8673055189749732
1101	26-27	0.8740054178061456
1101	28-29	0.8753770010447255
1101	30-31	0.8831536184548199
1101	32-33	0.8917255697054927
1101	34-35	0.9382323672833479
1101	36-37	0.930444776399284
1101	38-39	0.9145552801174475
1101	40-41	0.9043737084071708
1101	42-43	0.9163809621331112
1101	44-45	0.8899703936438428
1101	46-47	0.9614543932987445
1101	48-49	0.9498062437765356
1101	50-51	0.8909317191568462
1101	52-53	0.8456081470629329
1101	54-55	0.8169621993945029
1101	56-57	0.8171326558137935
1101	58-59	0.7842273088384104
1101	60-61	0.7346011620533375
1101	62-63	0.7387006153103002
1101	64-65	0.7604265706620481
1101	66-67	0.7025172107493844
1101	68-69	0.6536377953086046
1101	70-71	0.73963154870275
1101	72-73	0.7015905726544887
1101	74-75	0.6876512680527718
1101	76-77	0.6812190831625955
1101	78-79	0.6551110935557922
1101	80-81	0.6144225904481893
1101	82-83	0.5544426508678235
1101	84-85	0.6098921949597873
1101	86-87	0.6497739621468632
1101	88-89	0.6688024984809324
1101	90-91	0.6830762134439787
1101	92-93	0.6864560400182391
1101	94-95	0.6225892100049357
1101	96-97	0.6286750907456522
1101	98-99	0.6603825654615498
1101	100-101	0.6235400430689175
1102	1	0.6145666106361602
1102	2	0.5807104119244215
1102	3	0.601468700127505
1102	4	0.6273091161370701
1102	5	0.6513998766128779
1102	6	0.5886907988708145
1102	7	0.6015139833562628
1102	8	0.5831572493741533
1102	9	0.7093630565336184
1102	10-11	0.6942700851792694
1102	12-13	0.6812931651995981
1102	14-15	0.8203863527004955
1102	16-17	0.8205507250714206
1102	18-19	0.8415908636469709
1102	20-21	0.7996164240776196
1102	22-23	0.8152941380571193
1102	24-25	0.8173630064217718
1102	26-27	0.8430627343435404
1102	28-29	0.7998691610203608
1102	30-31	0.8186308893585306
1102	32-33	0.8402235776801703
1102	34-35	0.8530123435058741
1102	36-37	0.8238781818820513
1102	38-39	0.8518493273135945
1102	40-41	0.8712202587150273
1102	42-43	0.8217982664289494
1102	44-45	0.8162084011728084
1102	46-47	0.8731153694838483
1102	48-49	0.9149381066289877
1102	50-51	0.8673519347993235
1102	52-53	0.7615785774048618
1102	54-55	0.7072758298033719
1102	56-57	0.7521402364541885
1102	58-59	0.7795690271044151
1102	60-61	0.8101105378158309
1102	62-63	0.8096130299191557
1102	64-65	0.7432783505452676
1102	66-67	0.7483743837014813
1102	68-69	0.7519885301062601
1102	70-71	0.7532093238904913
1102	72-73	0.7208834419863095
1102	74-75	0.6786296437131618
1102	76-77	0.7059299305272724
1102	78-79	0.6660168143724015
1102	80-81	0.6672970736441179
1102	82-83	0.6630640668210503
1102	84-85	0.6316393859733864
1102	86-87	0.694091013381346
1102	88-89	0.7165222398622468
1102	90-91	0.7175058653419342
1102	92-93	0.7000416410675356
1102	94-95	0.655830117450904
1102	96-97	0.7147362211888186
1102	98-99	0.7402198691541741
1102	100-101	0.7382962922807437
1103	1	0.44390056588225235
1103	2	0.36668963738075533
1103	3	0.4115839505923091
1103	4	0.46717741995853146
1103	5	0.4771369514926391
1103	6	0.458504858709361
1103	7	0.5061218392687223
1103	8	0.4742965511845725
1103	9	0.5469418639072074
1103	10-11	0.5224927343271943
1103	12-13	0.5040556242963845
1103	14-15	0.6164284552886272
1103	16-17	0.6107805573400285
1103	18-19	0.6626683084424769
1103	20-21	0.6717375912748125
1103	22-23	0.6142485089941374
1103	24-25	0.6117935985934153
1103	26-27	0.6652796935509571
1103	28-29	0.62582809979655
1103	30-31	0.6392298192536998
1103	32-33	0.6184793365201386
1103	34-35	0.6344701607043746
1103	36-37	0.6565304574466779
1103	38-39	0.6745021567892451
1103	40-41	0.645091504292786
1103	42-43	0.6029352846201803
1103	44-45	0.5895591460958158
1103	46-47	0.622852633844488
1103	48-49	0.5981196198748577
1103	50-51	0.5993607567829216
1103	52-53	0.5561277584818924
1103	54-55	0.5108618611952309
1103	56-57	0.534493394896927
1103	58-59	0.5501344973581865
1103	60-61	0.5430856166101137
1103	62-63	0.5284180622569181
1103	64-65	0.5455620539518833
1103	66-67	0.5098091326306999
1103	68-69	0.4839060013325991
1103	70-71	0.4903277149891956
1103	72-73	0.5328114609547754
1103	74-75	0.4783318743863525
1103	76-77	0.47486386992181906
1103	78-79	0.4665058203114434
1103	80-81	0.4572578914232608
1103	82-83	0.43882444899928785
1103	84-85	0.42156763377750295
1103	86-87	0.44880441777582547
1103	88-89	0.44044750133871347
1103	90-91	0.444065327008893
1103	92-93	0.44026527997678144
1103	94-95	0.44272108711984615
1103	96-97	0.4755446306576303
1103	98-99	0.46042034359438233
1103	100-101	0.46281040888640845
1104	1	0.4077991948148849
1104	2	0.3329423451740894
1104	3	0.4167096230285736
1104	4	0.4492828475277051
1104	5	0.48752149553964585
1104	6	0.39205236560772505
1104	7	0.42376516879701853
1104	8	0.4528329270018858
1104	9	0.5326676317923571
1104	10-11	0.4553672055941149
1104	12-13	0.4594268265900183
1104	14-15	0.5450032292270777
1104	16-17	0.5765034393982944
1104	18-19	0.5887268924357301
1104	20-21	0.5720122855524679
1104	22-23	0.5571452496672933
1104	24-25	0.5683791476768931
1104	26-27	0.5956535523769517
1104	28-29	0.5829784767833601
1104	30-31	0.5840323183355594
1104	32-33	0.5782898438000501
1104	34-35	0.6021392743957605
1104	36-37	0.5970202464689578
1104	38-39	0.5919565192810836
1104	40-41	0.5695348357142151
1104	42-43	0.5456461956626626
1104	44-45	0.5088670470418535
1104	46-47	0.5895285533572334
1104	48-49	0.5922976718921475
1104	50-51	0.551601492159925
1104	52-53	0.5210117027204006
1104	54-55	0.4520224815583589
1104	56-57	0.4583994537721736
1104	58-59	0.5214338422140514
1104	60-61	0.5156890597934165
1104	62-63	0.5660848763454354
1104	64-65	0.5610443580450237
1104	66-67	0.5464870800562949
1104	68-69	0.5216478518529684
1104	70-71	0.5264617226657258
1104	72-73	0.5354037687795454
1104	74-75	0.5217927188930602
1104	76-77	0.4861676264289976
1104	78-79	0.5255416086215057
1104	80-81	0.519953032370637
1104	82-83	0.48828066034244344
1104	84-85	0.44206641685731896
1104	86-87	0.5070455739895081
1104	88-89	0.5371228726419766
1104	90-91	0.472136257899507
1104	92-93	0.48844102443642967
1104	94-95	0.46466794682055124
1104	96-97	0.48370283258626756
1104	98-99	0.5372006237099711
1104	100-101	0.5234388463033781
1105	1	0.3451862540730559
1105	2	0.35866556038762454
1105	3	0.33894341757611457
1105	4	0.3681573871582131
1105	5	0.4099455501126812
1105	6	0.2998037662402737
1105	7	0.3008082626330193
1105	8	0.2920869630645129
1105	9	0.41459165233773376
1105	10-11	0.298312350899387
1105	12-13	0.31110658055651186
1105	14-15	0.30327765765806447
1105	16-17	0.3606576590747892
1105	18-19	0.4313514446174196
1105	20-21	0.3605355278765572
1105	22-23	0.3355956032760119
1105	24-25	0.3572583107256264
1105	26-27	0.3626518064075199
1105	28-29	0.2971990579614001
1105	30-31	0.357850094335717
1105	32-33	0.3158552504154102
1105	34-35	0.40257565817704943
1105	36-37	0.39595856165846044
1105	38-39	0.4147085562387858
1105	40-41	0.3822542822252899
1105	42-43	0.3267726611230728
1105	44-45	0.393794232438232
1105	46-47	0.44081104737888666
1105	48-49	0.41657029984569505
1105	50-51	0.3491604271452644
1105	52-53	0.3409708868646817
1105	54-55	0.3209171618561655
1105	56-57	0.2698299035524485
1105	58-59	0.2418562278380918
1105	60-61	0.3139967675916324
1105	62-63	0.31798879148635706
1105	64-65	0.31810883232648735
1105	66-67	0.23619626292554585
1105	68-69	0.23231667680545343
1105	70-71	0.26047984067969665
1105	72-73	0.25658700524164146
1105	74-75	0.2746512861246977
1105	76-77	0.25892193355736737
1105	78-79	0.23383488773690786
1105	80-81	0.237943361941646
1105	82-83	0.25718769060777547
1105	84-85	0.19115226226050552
1105	86-87	0.2649511021768376
1105	88-89	0.23637571255675383
1105	90-91	0.22739747057028836
1105	92-93	0.12657649192543374
1105	94-95	0.20330529435752354
1105	96-97	0.1821309201936998
1105	98-99	0.1345062598454625
1105	100-101	0.1478027353171143
1106	1	0.29529580158271074
1106	2	0.27743594498015156
1106	3	0.35131164860281316
1106	4	0.31557682647039087
1106	5	0.33590203792293494
1106	6	0.24508093361769312
1106	7	0.3063392376503984
1106	8	0.3153406764105
1106	9	0.3459009572660392
1106	10-11	0.2685244589586304
1106	12-13	0.30720807275579887
1106	14-15	0.3385113292371571
1106	16-17	0.3586801415442338
1106	18-19	0.39920706317911225
1106	20-21	0.403574938043036
1106	22-23	0.41313987870174884
1106	24-25	0.39542005731520646
1106	26-27	0.34395163619396385
1106	28-29	0.37255347386818727
1106	30-31	0.37803418338396
1106	32-33	0.39585690872173274
1106	34-35	0.3622463037484138
1106	36-37	0.35571954057520117
1106	38-39	0.37763291650443875
1106	40-41	0.3659008167411528
1106	42-43	0.3738260966567708
1106	44-45	0.35082433409282743
1106	46-47	0.4449829652927306
1106	48-49	0.3679143918397756
1106	50-51	0.38700049948243276
1106	52-53	0.33770244445714326
1106	54-55	0.33596026534886647
1106	56-57	0.3957685587190909
1106	58-59	0.426356320615227
1106	60-61	0.35856440493137853
1106	62-63	0.3145192870212057
1106	64-65	0.3131028977586823
1106	66-67	0.3609580059883797
1106	68-69	0.3526159155283466
1106	70-71	0.3600833148771905
1106	72-73	0.41289409679953337
1106	74-75	0.31113917525425805
1106	76-77	0.3703134578891252
1106	78-79	0.3324142140415596
1106	80-81	0.3025274880602957
1106	82-83	0.3877980091639053
1106	84-85	0.3467930770786012
1106	86-87	0.34518277235672556
1106	88-89	0.34693293612956566
1106	90-91	0.36173672959195
1106	92-93	0.36310261099159646
1106	94-95	0.32744672308795586
1106	96-97	0.3840369742464347
1106	98-99	0.28270363252542907
1106	100-101	0.3199369589911534
1107	1	0.19432334162599219
1107	2	0.1489417903882888
1107	3	0.1812561019769916
1107	4	0.18194098886789334
1107	5	0.165170782474668
1107	6	0.09194742510748455
1107	7	0.1083203253001983
1107	8	0.14171418326916552
1107	9	0.1962979729847234
1107	10-11	0.11043022398929736
1107	12-13	0.11358747191366803
1107	14-15	0.061133751403673386
1107	16-17	0.10483601401237053
1107	18-19	0.13612197425171502
1107	20-21	0.1647210077241752
1107	22-23	0.13359255985267993
1107	24-25	0.13669259376843712
1107	26-27	0.0982701705912632
1107	28-29	0.1176208210867955
1107	30-31	0.14700473273349957
1107	32-33	0.04027949876813608
1107	34-35	0.1150005079866574
1107	36-37	0.1285060540105576
1107	38-39	0.16134746992192106
1107	40-41	0.08894355762497241
1107	42-43	0.11019796478294097
1107	44-45	0.13776832877584155
1107	46-47	0.12623175797705244
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2216	90-91	-1.2910884702061445
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2216	94-95	-1.5402917629486872
2216	96-97	-1.5793462520957853
2216	98-99	-1.5712157448080681
2216	100-101	-1.779208447837739
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	397600.0
3	47523.0
4	37885.0
5	35099.0
6	36313.0
7	37838.0
8	40626.0
9	44430.0
10	45803.0
11	47841.0
12	49081.0
13	49695.0
14	50579.0
15	52245.0
16	52820.0
17	54281.0
18	54556.0
19	54769.0
20	56360.0
21	60371.0
22	67801.0
23	82321.0
24	103436.0
25	128689.0
26	159519.0
27	198350.0
28	245500.0
29	304007.0
30	380148.0
31	484057.0
32	627747.0
33	820804.0
34	1119382.0
35	1647609.0
36	2742797.0
37	3599142.0
38	2450632.0
39	297516.0
40	137.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	38.48951436751661	17.551224068353203	6.5508233832668585	37.40843818086332
2	31.604735763990032	16.426752869619826	26.842749957332067	25.12576140905807
3	30.44344532509508	16.53536948202093	18.76174109588141	34.259444097002586
4	33.60049509246522	19.92630112427247	18.22330989882127	28.249893884441036
5	34.25578404288898	22.018174602439903	20.26878774368501	23.457253610986108
6	29.964801970396866	26.79021263647187	21.14571124237667	22.099274150754592
7	24.39913558016474	22.534324721725223	32.05208689135857	21.01445280675147
8	22.853001497375697	21.332243304826594	30.72838180637465	25.086373391423063
9	22.278084804967353	19.349539972885406	34.061776793283876	24.31059842886337
10-11	25.94137555175056	26.37000052900069	24.8937600231219	22.794863896126845
12-13	26.08899862198426	24.4054215731745	24.609023577760876	24.896556227080364
14-15	25.867544666929383	24.512788549282167	25.58111843491446	24.038548348873995
16-17	26.844461448151673	23.842620974684696	24.99431589505079	24.31860168211284
18-19	27.152861057989153	23.45278205774971	25.018761474201632	24.375595410059507
20-21	26.878209585803624	24.091685724049103	24.41786566875282	24.61223902139445
22-23	27.095324664325354	24.26709620087876	23.498888485855467	25.138690648940415
24-25	25.833962672623258	24.093658828689364	24.41472640796073	25.65765209072665
26-27	25.869092711876746	23.802509298303338	23.989268578882605	26.33912941093731
28-29	25.7439216010709	24.118796048872696	23.33118392549569	26.806098424560716
30-31	26.220932464419754	24.309143354159954	23.734571787613575	25.735352393806714
32-33	25.124345819874794	23.76402808714688	24.92266859315947	26.188957499818855
34-35	25.856103678176005	24.052211547570405	23.997390866216783	26.094293908036807
36-37	26.488589339103218	23.38961569127407	24.131695479216457	25.990099490406248
38-39	25.909717572820934	23.82966723489373	23.911793701708177	26.34882149057716
40-41	26.805615323428682	24.37860692974938	23.541368540798672	25.274409206023265
42-43	26.470190916535476	23.96272278369845	24.06864068321351	25.498445616552566
44-45	27.434346226718326	23.940329071232224	23.526536490964133	25.098788211085317
46-47	26.65332028233139	23.964198219949616	23.792809707392767	25.589671790326225
48-49	26.148026827768224	24.473501519508847	23.90301725659117	25.475454396131763
50-51	25.840484680467146	24.584292811526513	24.382528766278355	25.192693741727982
52-53	25.6395014898262	24.84279964732157	24.109673958851236	25.408024904000992
54-55	26.35829490726386	24.06758809563943	23.840207502268935	25.733909494827774
56-57	24.904491416268744	24.087288720312262	24.904640811832223	26.103579051586767
58-59	24.963009772805623	25.404242390580766	24.119740449503226	25.51300738711038
60-61	25.227208578949927	25.326763353600928	23.93571834755865	25.51030971989049
62-63	25.602169807359516	25.255778848442674	23.947854571742923	25.194196772454884
64-65	26.908830890965785	24.122194690713833	23.870751775869916	25.098222642450462
66-67	26.611735067999465	23.813281752490095	23.574192428312337	26.000790751198107
68-69	26.53121327027554	23.51046484337229	24.045079812430117	25.913242073922056
70-71	25.804959375387753	24.103303195780125	24.195612779104952	25.89612464972717
72-73	26.211694327160632	24.672200916725945	23.568228222500135	25.547876533613284
74-75	26.700964181027516	25.23086797803237	22.78732020700881	25.2808476339313
76-77	26.728999955009474	24.456703549537405	23.4109695745145	25.403326920938618
78-79	25.983357343197543	24.378224579731782	24.21705347433221	25.421364602738468
80-81	26.710108423266348	24.537236945718586	24.029735033822657	24.72291959719241
82-83	26.518818650249877	24.235601236009593	23.986685707967652	25.25889440577288
84-85	26.63165713017487	24.622850928883825	22.8903672596064	25.855124681334907
86-87	27.013017511598814	24.73466697488704	22.68128066938822	25.57103484412593
88-89	26.751591253238722	24.23248928471324	22.814686651489914	26.20123281055813
90-91	26.814095598663446	23.92137950851333	23.440097177234836	25.82442771558839
92-93	26.97335196159877	24.55406722605233	23.512661753894804	24.9599190584541
94-95	26.92622025511317	25.669742362578184	23.009472491705665	24.39456489060298
96-97	27.309104044468384	25.251540754058066	22.98166528737146	24.45768991410209
98-99	26.99955540886893	25.54362925049333	22.68987463641507	24.766940704222673
100-101	26.75406748467357	25.96992417875249	22.583728627673054	24.692279708900887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3332.0
1	2973.0
2	2671.0
3	2529.0
4	2194.0
5	1983.0
6	1827.0
7	1672.5
8	1576.0
9	1406.0
10	1235.0
11	1163.5
12	1077.5
13	1141.0
14	1160.5
15	1342.5
16	1777.5
17	2495.5
18	3145.5
19	3759.0
20	4506.0
21	5368.0
22	6592.5
23	8041.0
24	9903.5
25	12186.0
26	15913.0
27	20476.5
28	23723.0
29	28974.5
30	40229.5
31	47132.0
32	50960.5
33	66181.0
34	83812.5
35	109430.0
36	150251.0
37	179254.0
38	221113.5
39	287496.5
40	356201.5
41	434762.5
42	499841.0
43	533003.0
44	586300.5
45	641512.0
46	695422.0
47	739895.5
48	737506.5
49	719267.5
50	676300.0
51	617397.5
52	583464.5
53	554318.0
54	496588.5
55	458967.5
56	435198.5
57	408435.5
58	400403.5
59	431188.0
60	440772.0
61	427757.0
62	459424.5
63	493281.0
64	440811.0
65	316710.0
66	220220.5
67	188114.0
68	182986.0
69	143051.0
70	100443.0
71	81824.5
72	70364.0
73	59201.0
74	52815.5
75	63449.5
76	68221.0
77	66743.5
78	67258.5
79	53650.0
80	41219.0
81	41393.0
82	42952.5
83	38300.5
84	31892.0
85	30802.0
86	32297.5
87	28293.5
88	19678.5
89	12515.0
90	9048.5
91	7287.0
92	5548.5
93	4032.0
94	2586.0
95	1526.5
96	925.5
97	589.0
98	404.0
99	262.5
100	225.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5300886491265983
2	0.11757015632697257
3	0.1425503102865566
4	0.12985146888733157
5	0.12892097604643016
6	0.12808591836869812
7	0.13155737242898416
8	0.13131878452106072
9	0.13739681147541033
10-11	0.15627806203870148
12-13	0.1508889576684808
14-15	0.14003022550911529
16-17	0.12673789668893068
18-19	0.14446796059649125
20-21	0.15740240755479068
22-23	0.13618597784269887
24-25	0.1522846969298329
26-27	0.17652224602600525
28-29	0.14472742494635799
30-31	0.16889936236785139
32-33	0.1473161037473273
34-35	0.4227747904914845
36-37	0.13983339048507845
38-39	0.14348378547630708
40-41	0.14683296323378234
42-43	0.25602271929494413
44-45	0.1442979667120958
46-47	0.17858304908069395
48-49	0.2827684237731616
50-51	0.13411324539261402
52-53	0.1617327780836011
54-55	0.17591086451195143
56-57	0.1861641798549612
58-59	0.1798087944576506
60-61	0.18073332260085395
62-63	0.20752077996295804
64-65	0.16292571762321828
66-67	0.15982407482021357
68-69	0.16049510331124825
70-71	0.18084068715941948
72-73	0.18474458180281678
74-75	0.17595559974468708
76-77	0.169707578905942
78-79	0.16962407313816882
80-81	0.16308974680991564
82-83	0.171231559167803
84-85	0.15828220046525834
86-87	0.15629595613179573
88-89	0.15313168400296112
90-91	0.17470897792578713
92-93	0.16286010594853934
94-95	0.17678469272472103
96-97	0.17359954415394313
98-99	0.17719625686588897
100-101	0.1468448926291785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6765309E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.83086147320484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.13815871514792	35.579260875283424
2	8.271903273754603	6.754978733808476
3	1.9312671143944593	2.365659000467886
4	0.7478911511581132	1.2214815995989048
5	0.4065866246136007	0.8300641073227933
6	0.26553860403414364	0.6505301974263786
7	0.16502712370162687	0.4716739739027796
8	0.1189205169608365	0.38845017234798573
9	0.08829692948291364	0.3244715726603564
>10	0.6233893616546134	5.067766156250668
>50	0.09186522676642186	2.652283310064393
>100	0.10715457328142453	9.584358553051393
>500	0.021108733148232646	6.026751629665959
>1k	0.020260284585620725	16.791926908282438
>5k	0.0017349080627857678	4.777924943298214
>10k+	8.968592527960325E-4	6.512418266568172
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC	44853	0.26753458585225	No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT	43436	0.2590826092140622	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT	43215	0.2577644110227852	No Hit
CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC	40104	0.23920823648403977	No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT	36203	0.21593995076380637	No Hit
GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG	29020	0.17309552719845486	No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT	28192	0.16815675750443967	No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG	26335	0.15708031387909402	No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG	25338	0.15113351027410232	No Hit
CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA	24481	0.14602176434684264	No Hit
GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC	23965	0.14294398033463027	No Hit
CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA	23673	0.14120228860678918	No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG	23566	0.14056406595309398	No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA	23175	0.13823186915314237	No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT	22064	0.13160509001056886	No Hit
CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA	21533	0.12843783553288518	No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG	21307	0.12708981385311777	No Hit
CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA	20358	0.12142931573763417	No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC	19375	0.11556601790041568	No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA	17833	0.10636845404996711	No Hit
GTCCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGC	17482	0.10427484515793893	No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT	17413	0.103863281016771	No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG	17074	0.10184124849711987	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.543516316937552E-5	5.96469769808597E-6	0.0	1.192939539617194E-5	0.0
2	9.543516316937552E-5	3.578818618851582E-5	5.96469769808597E-6	1.789409309425791E-5	0.0
3	9.543516316937552E-5	4.771758158468776E-5	5.96469769808597E-6	1.789409309425791E-5	0.0
4	1.0736455856554746E-4	7.157637237703164E-5	5.96469769808597E-6	2.385879079234388E-5	0.0
5	1.4911744245214926E-4	7.754107007511762E-5	5.96469769808597E-6	2.385879079234388E-5	0.0
6	1.6701153554640717E-4	8.350576777320358E-5	5.96469769808597E-6	3.578818618851582E-5	0.0
7	1.8490562864066507E-4	1.0736455856554746E-4	5.96469769808597E-6	3.578818618851582E-5	0.0
8	2.743760941119546E-4	1.192939539617194E-4	5.96469769808597E-6	4.771758158468776E-5	0.0
9	3.817406526775021E-4	1.192939539617194E-4	5.96469769808597E-6	4.771758158468776E-5	0.0
10-11	5.9945211865764E-4	1.192939539617194E-4	5.96469769808597E-6	4.771758158468776E-5	0.0
12-13	8.499694219772508E-4	1.192939539617194E-4	5.96469769808597E-6	5.368227928277373E-5	2.982348849042985E-6
14-15	0.0010855749810516468	1.3420569820693433E-4	5.96469769808597E-6	5.96469769808597E-5	5.96469769808597E-6
16-17	0.0014673156337291487	1.6402918669736416E-4	5.96469769808597E-6	6.262932582990269E-5	5.96469769808597E-6
18-19	0.0021025559385753046	1.6701153554640717E-4	5.96469769808597E-6	7.455872122607463E-5	8.947046547128955E-6
20-21	0.002794460871553277	1.6701153554640717E-4	5.96469769808597E-6	7.754107007511762E-5	1.192939539617194E-5
22-23	0.004777722856166862	1.6701153554640717E-4	5.96469769808597E-6	8.350576777320358E-5	1.192939539617194E-5
24-25	0.009474922293409564	1.7297623324449314E-4	5.96469769808597E-6	8.947046547128955E-5	1.192939539617194E-5
26-27	0.016799571066659136	1.7894093094257912E-4	5.96469769808597E-6	9.543516316937552E-5	1.192939539617194E-5
28-29	0.02880054283520811	1.819232797916221E-4	5.96469769808597E-6	9.543516316937552E-5	1.192939539617194E-5
30-31	0.046130971996996895	1.9087032633875105E-4	5.96469769808597E-6	1.013998608674615E-4	1.192939539617194E-5
32-33	0.0684926236671212	1.9683502403683702E-4	5.96469769808597E-6	1.1332925626363344E-4	1.192939539617194E-5
34-35	0.09462098193358678	1.9683502403683702E-4	8.947046547128955E-6	1.222763028107624E-4	1.192939539617194E-5
36-37	0.12556285124240776	2.0578207058396598E-4	1.192939539617194E-5	1.3420569820693433E-4	1.192939539617194E-5
38-39	0.16200118948001496	2.1771146598013793E-4	1.192939539617194E-5	1.4613509360310626E-4	1.192939539617194E-5
40-41	0.2046935132540653	2.206938148291809E-4	1.192939539617194E-5	1.5508214015023524E-4	1.192939539617194E-5
42-43	0.250878167530345	2.206938148291809E-4	1.192939539617194E-5	1.5508214015023524E-4	1.192939539617194E-5
44-45	0.30025393507510056	2.475349544705678E-4	1.192939539617194E-5	1.580644889992782E-4	1.192939539617194E-5
46-47	0.35285362172567175	2.6841139641386867E-4	1.192939539617194E-5	1.7894093094257912E-4	1.192939539617194E-5
48-49	0.41743042135399955	2.743760941119546E-4	1.192939539617194E-5	1.8788797748970807E-4	1.192939539617194E-5
50-51	0.48818068309984625	2.743760941119546E-4	1.192939539617194E-5	1.9683502403683702E-4	1.192939539617194E-5
52-53	0.5640516378194997	2.8928783835716954E-4	1.192939539617194E-5	2.0876441943300895E-4	1.789409309425791E-5
54-55	0.6516372588181941	2.952525360552555E-4	1.789409309425791E-5	2.0876441943300895E-4	1.789409309425791E-5
56-57	0.7550502051587596	3.191113268475994E-4	1.789409309425791E-5	2.0876441943300895E-4	2.0876441943300896E-5
58-59	0.873571730768577	3.548995130361152E-4	1.789409309425791E-5	2.1174676828205192E-4	2.982348849042985E-5
60-61	1.0064890542727247	3.787583038284591E-4	1.789409309425791E-5	2.1472911713109493E-4	3.578818618851582E-5
62-63	1.1617501353539026	3.96652396922717E-4	1.789409309425791E-5	2.1472911713109493E-4	3.578818618851582E-5
64-65	1.3413889359271578	4.175288388660179E-4	2.0876441943300896E-5	2.1472911713109493E-4	3.578818618851582E-5
66-67	1.5456321145050174	4.264758854131469E-4	2.385879079234388E-5	2.1472911713109493E-4	3.578818618851582E-5
68-69	1.7868534364621613	4.3244058311123283E-4	2.385879079234388E-5	2.2665851252726685E-4	3.578818618851582E-5
70-71	2.05049605706641	4.413876296583618E-4	2.385879079234388E-5	2.4455260562152476E-4	3.578818618851582E-5
72-73	2.338927961303904	4.4735232735644776E-4	2.385879079234388E-5	2.713937452629117E-4	3.578818618851582E-5
74-75	2.6577410532665997	4.5033467620549073E-4	2.385879079234388E-5	2.982348849042985E-4	3.578818618851582E-5
76-77	3.0250232787239413	4.5331702505453376E-4	2.385879079234388E-5	3.0419958260238453E-4	3.877053503755881E-5
78-79	3.4450751847162495	4.592817227526197E-4	2.385879079234388E-5	3.191113268475994E-4	4.175288388660179E-5
80-81	3.910652645889199	4.6524642045070566E-4	2.385879079234388E-5	3.3402307109281433E-4	4.175288388660179E-5
82-83	4.446887915993675	4.6524642045070566E-4	2.385879079234388E-5	3.6086421073420116E-4	4.175288388660179E-5
84-85	5.066491765824298	4.6524642045070566E-4	2.385879079234388E-5	3.8472300152654507E-4	4.175288388660179E-5
86-87	5.723536023105808	4.6524642045070566E-4	2.982348849042985E-5	3.8770535037558804E-4	4.175288388660179E-5
88-89	6.418044546628995	4.6524642045070566E-4	2.982348849042985E-5	3.8770535037558804E-4	4.175288388660179E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGGA	5345	0.0	47.69567	2
GGGCGAT	7830	0.0	37.84798	1
GGCGATC	7995	0.0	36.51817	2
GGCGCGT	9475	0.0	34.069862	5
CGGCATT	16665	0.0	32.419395	3
GCGATCT	10360	0.0	29.282562	3
GCGTGCC	11130	0.0	29.039219	8
GCATTCT	19820	0.0	27.496597	5
TAAAACG	15245	0.0	27.142109	58-59
ACGCTCT	15260	0.0	26.865118	62-63
CGAAATT	15705	0.0	26.479483	52-53
TCAACGA	15990	0.0	26.112123	48-49
GGCATTC	21340	0.0	26.04864	4
TCGGGAA	3085	0.0	25.545881	3
AACGCAC	16075	0.0	25.48353	72-73
CGCGTGC	12845	0.0	25.051128	7
AACGAAA	16745	0.0	24.891212	50-51
CGTGCCT	13370	0.0	24.63548	9
CCGGCAT	21740	0.0	24.632132	2
CGGTGGC	14000	0.0	24.46893	1
>>END_MODULE