FastQCFastQC Report
Thu 26 May 2016
SRR2048277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16765309
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC930300.5548958268529378TruSeq Adapter, Index 7 (97% over 35bp)
CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC479910.2862518072288438No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT472280.2817007428852042No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT398580.2377409208503106No Hit
GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC388860.23194323468777103No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT386600.23059521300800362No Hit
GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG325970.19443125086450835No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT301520.17984756499268817No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG284940.16995809620926164No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT277750.16566947856433784No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG264180.15757538378803515No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA255960.1526724022802085No Hit
CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA244110.14560423550797663No Hit
CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA234290.13974690236845622No Hit
GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC226580.13514812044323193No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG225730.13464112113889462No Hit
CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA206700.123290301419437No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC203890.12161422136627484No Hit
GTCCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGC193870.1156375942727927No Hit
CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA193090.115172347852342No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA185760.11080022443964498No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG181980.10854556870976849No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT174890.10431659804182553No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT173300.10336821110782987No Hit
CTCGCTAATTTGACTATGGATTCATCAAAATGCAACTGAGGTTTGCTCAG170090.10145354314674428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT76950.037.5722121
GGCGATC79800.035.305622
CGGCATT149900.032.7048233
GGCGCGT88100.031.5437765
GCGATCT106200.027.6885283
TAAAACG137550.027.22821258-59
GCATTCT181550.026.8463255
CGCTAAT64950.026.8423583
ACGCTCT138550.026.65458362-63
GCGTGCC105150.026.6514478
CGAAATT141800.026.34514252-53
GGCATTC192850.025.8890914
CCCGTTA179350.025.6652661
TCAACGA147700.025.48571448-49
AACGAAA148900.025.24842350-51
AACGCAC146450.024.97357272-73
GCCGTCT211450.024.85545748-49
CTTGAAA210150.024.839760-61
TCGCTAA71050.024.8085782
GCTTGAA218250.024.38572358-59
Produced by FastQC (version 0.11.5)