##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2048277_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16765309
Sequences flagged as poor quality	0
Sequence length	101
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08954931877486	33.0	31.0	34.0	30.0	34.0
2	32.2040178919458	34.0	31.0	34.0	30.0	34.0
3	32.299797695348175	34.0	31.0	34.0	30.0	34.0
4	35.90413889776801	37.0	35.0	37.0	35.0	37.0
5	35.71695630542807	37.0	35.0	37.0	33.0	37.0
6	35.67979534406434	37.0	35.0	37.0	33.0	37.0
7	35.64708691023828	37.0	35.0	37.0	33.0	37.0
8	35.69712052429215	37.0	35.0	37.0	33.0	37.0
9	37.31820141221375	39.0	37.0	39.0	34.0	39.0
10-11	37.37118889368517	39.0	37.0	39.0	34.0	39.0
12-13	37.34873386467258	39.0	37.0	39.0	34.0	39.0
14-15	38.641107151678504	40.0	38.0	41.0	34.0	41.0
16-17	38.61116228158992	40.0	38.0	41.0	34.0	41.0
18-19	38.59876337501444	40.0	38.0	41.0	34.0	41.0
20-21	38.54492076465755	40.0	38.0	41.0	34.0	41.0
22-23	38.492698732841724	40.0	38.0	41.0	34.0	41.0
24-25	38.39590958329488	40.0	38.0	41.0	33.5	41.0
26-27	38.265665726769484	40.0	38.0	41.0	33.0	41.0
28-29	38.15588949180716	40.0	38.0	41.0	33.0	41.0
30-31	38.039201663387175	40.0	37.0	41.0	33.0	41.0
32-33	37.91513651791327	40.0	37.0	41.0	33.0	41.0
34-35	37.8267983608295	40.0	37.0	41.0	33.0	41.0
36-37	37.666956570857124	40.0	37.0	41.0	32.0	41.0
38-39	37.53834662397216	40.0	36.5	41.0	32.0	41.0
40-41	37.32569441457954	40.0	36.0	41.0	31.5	41.0
42-43	37.0917659793804	39.0	35.0	41.0	31.0	41.0
44-45	36.99856751820083	39.0	35.0	41.0	31.0	41.0
46-47	36.78719527328724	39.0	35.0	41.0	31.0	41.0
48-49	36.69057632638921	39.0	35.0	41.0	31.0	41.0
50-51	36.62754951310471	39.0	35.0	41.0	31.0	41.0
52-53	36.405247764893566	38.0	35.0	41.0	31.0	41.0
54-55	36.13013175003216	38.0	35.0	40.0	30.0	41.0
56-57	35.86120124597763	37.0	35.0	40.0	30.0	41.0
58-59	35.58612200347754	37.0	34.0	40.0	30.0	41.0
60-61	35.30251148368336	36.0	34.0	39.5	29.0	41.0
62-63	34.997710778846965	36.0	34.0	39.0	29.0	41.0
64-65	34.682471405686584	35.0	34.0	39.0	29.0	41.0
66-67	34.39621345481912	35.0	34.0	38.5	29.0	40.5
68-69	34.057912711301654	35.0	33.0	37.0	28.5	40.0
70-71	33.756372727755874	35.0	33.0	37.0	28.0	39.5
72-73	33.44861854320729	35.0	33.0	36.0	28.0	39.0
74-75	33.20098734833935	35.0	33.0	36.0	28.0	39.0
76-77	33.07293322180939	35.0	33.0	35.5	28.0	37.0
78-79	32.92446387358562	35.0	33.0	35.0	28.0	37.0
80-81	32.75720426626196	35.0	33.0	35.0	28.0	36.5
82-83	32.59094640009319	35.0	33.0	35.0	27.0	36.0
84-85	32.464441752907746	35.0	33.0	35.0	27.0	36.0
86-87	32.336092463312184	35.0	33.0	35.0	27.0	36.0
88-89	32.22477676969747	35.0	33.0	35.0	27.0	35.0
90-91	32.124780700433256	35.0	33.0	35.0	27.0	35.0
92-93	32.000438047398944	35.0	33.0	35.0	27.0	35.0
94-95	31.905380718005254	35.0	33.0	35.0	27.0	35.0
96-97	31.79142093354796	35.0	33.0	35.0	27.0	35.0
98-99	31.61162651401176	35.0	33.0	35.0	25.5	35.0
100-101	31.014667370580526	34.5	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.47339237647892674
1101	2	0.46300196769218616
1101	3	0.4609778709860848
1101	4	0.3021301961968774
1101	5	0.37347807095179775
1101	6	0.33199346154855647
1101	7	0.41555768206735166
1101	8	0.38393279826052407
1101	9	0.4725655577819907
1101	10-11	0.4664245847237538
1101	12-13	0.44298948933787585
1101	14-15	0.5706778519396281
1101	16-17	0.6136722773891066
1101	18-19	0.5730902809390486
1101	20-21	0.5892424571014345
1101	22-23	0.6166304331529275
1101	24-25	0.5845442538875787
1101	26-27	0.5785588303372862
1101	28-29	0.6243066692299024
1101	30-31	0.6456825293899442
1101	32-33	0.6132876336582456
1101	34-35	0.6441198275689146
1101	36-37	0.6597018436660989
1101	38-39	0.6893691811025136
1101	40-41	0.6600952992757101
1101	42-43	0.6879447697618346
1101	44-45	0.6611785476324457
1101	46-47	0.6681087916027906
1101	48-49	0.6534659255495328
1101	50-51	0.6702549229383337
1101	52-53	0.6322071447703905
1101	54-55	0.6154345417510356
1101	56-57	0.660813652685242
1101	58-59	0.5994722367407732
1101	60-61	0.5978182253609248
1101	62-63	0.5351748073556095
1101	64-65	0.555485465008168
1101	66-67	0.5577940062113171
1101	68-69	0.5537688390326991
1101	70-71	0.5572366825498207
1101	72-73	0.5468930286488174
1101	74-75	0.570572217407971
1101	76-77	0.5824155683958949
1101	78-79	0.5473866099174813
1101	80-81	0.5443862287481238
1101	82-83	0.5436465429093502
1101	84-85	0.5852317369604663
1101	86-87	0.5390612547755751
1101	88-89	0.5544362999057029
1101	90-91	0.5323964699301484
1101	92-93	0.5482340575879121
1101	94-95	0.589829349244436
1101	96-97	0.5816443959159301
1101	98-99	0.5556108334192267
1101	100-101	0.649421298176108
1102	1	0.4258370288399931
1102	2	0.4506106328714239
1102	3	0.4200318910859622
1102	4	0.28375130798412584
1102	5	0.3740841093403162
1102	6	0.34354171531969513
1102	7	0.3709604087966767
1102	8	0.3579594929250902
1102	9	0.4332282726481864
1102	10-11	0.4202325826338438
1102	12-13	0.4030870879327395
1102	14-15	0.5459920771483695
1102	16-17	0.5860242959759105
1102	18-19	0.5424672785215066
1102	20-21	0.5740341370437534
1102	22-23	0.5477957852647108
1102	24-25	0.5676649169564172
1102	26-27	0.5543014901684415
1102	28-29	0.605578501414989
1102	30-31	0.6114341270832
1102	32-33	0.608737857986867
1102	34-35	0.6179305689743515
1102	36-37	0.6684415496812903
1102	38-39	0.6615120115143256
1102	40-41	0.6435692996592692
1102	42-43	0.6532412748270531
1102	44-45	0.6205353193430909
1102	46-47	0.7138988679936702
1102	48-49	0.669571364187874
1102	50-51	0.6142702518028429
1102	52-53	0.5956566452092105
1102	54-55	0.606378236679241
1102	56-57	0.6213158537697367
1102	58-59	0.6133760748399197
1102	60-61	0.5998925469440621
1102	62-63	0.5573701911657167
1102	64-65	0.5789938523554596
1102	66-67	0.5523994923839552
1102	68-69	0.560431141476279
1102	70-71	0.5581924659446571
1102	72-73	0.5474534841890772
1102	74-75	0.5494242404481327
1102	76-77	0.5489209124778824
1102	78-79	0.5384682785406341
1102	80-81	0.5283020123478508
1102	82-83	0.5325222264466092
1102	84-85	0.5676074676497578
1102	86-87	0.5618628776618237
1102	88-89	0.5461655435209423
1102	90-91	0.5405653305202591
1102	92-93	0.5252574914800121
1102	94-95	0.5393600584450517
1102	96-97	0.5535858267437419
1102	98-99	0.6075831598195656
1102	100-101	0.6895744778765049
1103	1	0.3489185313889962
1103	2	0.3661174580844815
1103	3	0.31868322058575416
1103	4	0.24812479399373188
1103	5	0.25977200976539194
1103	6	0.30245445404553095
1103	7	0.30074169533381934
1103	8	0.3042676277190566
1103	9	0.3455851035443871
1103	10-11	0.3335632546943188
1103	12-13	0.35881601993758494
1103	14-15	0.48497978878394576
1103	16-17	0.4801761825295472
1103	18-19	0.4657063653695488
1103	20-21	0.5033293542759623
1103	22-23	0.4792895692884258
1103	24-25	0.49073760089063967
1103	26-27	0.4671551202741213
1103	28-29	0.5272960999579084
1103	30-31	0.45911817215909423
1103	32-33	0.5039037373107504
1103	34-35	0.49320356372930974
1103	36-37	0.5597947081185808
1103	38-39	0.5290675476008246
1103	40-41	0.4766716240677056
1103	42-43	0.5119115478403913
1103	44-45	0.5417552528785947
1103	46-47	0.6086445185874254
1103	48-49	0.5614301268362638
1103	50-51	0.5246337535795362
1103	52-53	0.4575672668122479
1103	54-55	0.5177173402575121
1103	56-57	0.5561200207234052
1103	58-59	0.543179869400646
1103	60-61	0.5575322214732381
1103	62-63	0.5225195510106033
1103	64-65	0.4936550189678641
1103	66-67	0.4591371513106779
1103	68-69	0.47416241388357605
1103	70-71	0.4530470210310895
1103	72-73	0.4520184740284705
1103	74-75	0.47303855813001405
1103	76-77	0.45616000635394727
1103	78-79	0.4265015384386288
1103	80-81	0.47430682472864305
1103	82-83	0.4515647752909544
1103	84-85	0.48141731711840663
1103	86-87	0.49116000164608664
1103	88-89	0.46973386772967274
1103	90-91	0.44837464762664325
1103	92-93	0.4732048222582961
1103	94-95	0.48339183713522615
1103	96-97	0.5260891943573682
1103	98-99	0.5793701032407874
1103	100-101	0.632145365948876
1104	1	0.26641602932944863
1104	2	0.2933079634053044
1104	3	0.2725465803170337
1104	4	0.1887550125395876
1104	5	0.21520493478270453
1104	6	0.2770263890922635
1104	7	0.2799080946569461
1104	8	0.2568136633793543
1104	9	0.32588081888918197
1104	10-11	0.3202371771335919
1104	12-13	0.3166762379993884
1104	14-15	0.43759733797818967
1104	16-17	0.4220753213052788
1104	18-19	0.40118746717567433
1104	20-21	0.37476638674558416
1104	22-23	0.40147783123475733
1104	24-25	0.4077773152802564
1104	26-27	0.40088059776554275
1104	28-29	0.38609288676731524
1104	30-31	0.3878482200866529
1104	32-33	0.42653468221002555
1104	34-35	0.4353414376970335
1104	36-37	0.43390382803662675
1104	38-39	0.4581612413827898
1104	40-41	0.3327440318951034
1104	42-43	0.4790459020148461
1104	44-45	0.46818257777665195
1104	46-47	0.5494958371381742
1104	48-49	0.47063172885257387
1104	50-51	0.41695435150842997
1104	52-53	0.41753827421624123
1104	54-55	0.4376014355192339
1104	56-57	0.3635303094425595
1104	58-59	0.4175368383381297
1104	60-61	0.43282238235070736
1104	62-63	0.39743437104749546
1104	64-65	0.3907762668582251
1104	66-67	0.34650723770203484
1104	68-69	0.36622321167450167
1104	70-71	0.3671480047021518
1104	72-73	0.3658451606426141
1104	74-75	0.4252147257306689
1104	76-77	0.38507153397410576
1104	78-79	0.39915738440492987
1104	80-81	0.38109491137903007
1104	82-83	0.3754315174181855
1104	84-85	0.4095429349407027
1104	86-87	0.4172748105725219
1104	88-89	0.42621245697570487
1104	90-91	0.405754233751523
1104	92-93	0.42964688634027226
1104	94-95	0.46545954511841714
1104	96-97	0.46369259680097485
1104	98-99	0.4980276798201224
1104	100-101	0.5336842476133974
1105	1	0.23480559117435718
1105	2	0.25994334773772465
1105	3	0.2427639532926662
1105	4	0.16339539319989171
1105	5	0.1563773517249345
1105	6	0.18057648975675278
1105	7	0.21337807851811164
1105	8	0.21069166474620005
1105	9	0.2438645829951227
1105	10-11	0.21685320136287345
1105	12-13	0.21942804345746225
1105	14-15	0.27019553048005207
1105	16-17	0.27580381714100355
1105	18-19	0.22009597768391842
1105	20-21	0.28709330690156776
1105	22-23	0.2883293822344797
1105	24-25	0.3117761208059022
1105	26-27	0.2832949662106401
1105	28-29	0.3088196888704289
1105	30-31	0.27880865050384074
1105	32-33	0.2568404555718047
1105	34-35	0.3008671359094919
1105	36-37	0.2792421207216549
1105	38-39	0.2934167837071371
1105	40-41	0.31745217122297475
1105	42-43	0.36097463921582573
1105	44-45	0.23026426371715303
1105	46-47	0.3197630721862481
1105	48-49	0.28938894279552585
1105	50-51	0.2655374542187232
1105	52-53	0.30360722397133344
1105	54-55	0.26175645090101085
1105	56-57	0.28520038146483984
1105	58-59	0.22253220097413617
1105	60-61	0.25154102630315833
1105	62-63	0.2587805097372069
1105	64-65	0.2590716131338624
1105	66-67	0.1726765290007677
1105	68-69	0.1606226946533411
1105	70-71	0.19273320768389368
1105	72-73	0.16293078147921136
1105	74-75	0.2264833028789326
1105	76-77	0.23739318143706356
1105	78-79	0.24655140180095003
1105	80-81	0.2098730710581549
1105	82-83	0.194211532680427
1105	84-85	0.20668307374166517
1105	86-87	0.19285836396996103
1105	88-89	0.2184124742923501
1105	90-91	0.17129509100622187
1105	92-93	0.1807188870294283
1105	94-95	0.15409659282152788
1105	96-97	0.18844028511178124
1105	98-99	0.23969113796563235
1105	100-101	0.3025967366480451
1106	1	0.1767660255205996
1106	2	0.19056107854463278
1106	3	0.1710870163272702
1106	4	0.1218955238448487
1106	5	0.13868389065530806
1106	6	0.15193595818651318
1106	7	0.20218033111812161
1106	8	0.18608895835445338
1106	9	0.21892470659118857
1106	10-11	0.1911682668711734
1106	12-13	0.18047955720876274
1106	14-15	0.2652789397055315
1106	16-17	0.2532954724721108
1106	18-19	0.251763720049091
1106	20-21	0.2516977412294281
1106	22-23	0.26692219235411585
1106	24-25	0.24911027151408405
1106	26-27	0.26630890005753827
1106	28-29	0.25546895810378345
1106	30-31	0.2531230659414234
1106	32-33	0.21704243764801845
1106	34-35	0.29062365139253643
1106	36-37	0.1949433986653446
1106	38-39	0.2928771046280758
1106	40-41	0.2171749709567905
1106	42-43	0.30719735371332746
1106	44-45	0.2729861384147867
1106	46-47	0.3432452829817976
1106	48-49	0.3037009647208819
1106	50-51	0.24554592093236494
1106	52-53	0.22650320532757462
1106	54-55	0.2623726601572187
1106	56-57	0.24134916780159443
1106	58-59	0.2686887796845028
1106	60-61	0.2851647086663931
1106	62-63	0.252989855544854
1106	64-65	0.1836812242396988
1106	66-67	0.2159651690573554
1106	68-69	0.2502598118884478
1106	70-71	0.2327337683819266
1106	72-73	0.22534806539765384
1106	74-75	0.27551982247466356
1106	76-77	0.23288320508104476
1106	78-79	0.2499590291644722
1106	80-81	0.2344938421928333
1106	82-83	0.16884139613754456
1106	84-85	0.20823425563427378
1106	86-87	0.2016960801908283
1106	88-89	0.2530363626846679
1106	90-91	0.2505093836594128
1106	92-93	0.24071107009758919
1106	94-95	0.26092115367203306
1106	96-97	0.24886325559846512
1106	98-99	0.2973012476420003
1106	100-101	0.2938153261820027
1107	1	0.019459958789987297
1107	2	0.08941187334175993
1107	3	0.10912369440670489
1107	4	0.05272028179349775
1107	5	-0.08659147200454953
1107	6	0.03202456723594338
1107	7	0.067867379623749
1107	8	0.06712632805434282
1107	9	0.11828119836960127
1107	10-11	0.08703864007917872
1107	12-13	0.10508240773527433
1107	14-15	0.15215017333591163
1107	16-17	0.06632349317715835
1107	18-19	0.0845296215528819
1107	20-21	0.11778052676248763
1107	22-23	0.09891778092940484
1107	24-25	0.0903108852727712
1107	26-27	0.14400827438807795
1107	28-29	0.10814995791191961
1107	30-31	0.07715171719948444
1107	32-33	0.05666116490499462
1107	34-35	0.14395991001799757
1107	36-37	0.15982811395885932
1107	38-39	0.19164226434574516
1107	40-41	0.15354788601581504
1107	42-43	0.17385657444866354
1107	44-45	0.1101780109991779
1107	46-47	0.1664534033489531
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2216	82-83	-0.906338712836952
2216	84-85	-0.9473225450476406
2216	86-87	-0.9266469662987973
2216	88-89	-0.8395611654732065
2216	90-91	-0.8780658362561127
2216	92-93	-0.8275017167108913
2216	94-95	-0.9792613209949614
2216	96-97	-1.1753148216633242
2216	98-99	-1.0199756485335918
2216	100-101	-1.167595318339469
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	337.0
3	485.0
4	783.0
5	1498.0
6	3086.0
7	6103.0
8	10288.0
9	13166.0
10	16093.0
11	19179.0
12	22420.0
13	23920.0
14	24724.0
15	25309.0
16	27182.0
17	29129.0
18	31318.0
19	34250.0
20	38038.0
21	43803.0
22	52244.0
23	64868.0
24	84948.0
25	112317.0
26	149326.0
27	201930.0
28	239697.0
29	290716.0
30	363782.0
31	453890.0
32	569800.0
33	733654.0
34	1006452.0
35	1540404.0
36	2657495.0
37	4090119.0
38	3254576.0
39	527419.0
40	561.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.61614575715697	17.416195305993917	6.546499792551557	36.421159144297555
2	30.958638347991656	16.565545585555963	27.142092040744476	25.33372402570791
3	29.939544323277268	16.153395356743967	19.478597748010383	34.42846257196838
4	33.356516125053226	20.178608100811026	18.401056610409032	28.063819163726716
5	34.002113872456505	22.045188005387338	20.450854075932988	23.50184404622317
6	29.351844622274157	27.17869520489053	21.645063992631783	21.82439618020353
7	23.514770115885277	23.19142469843135	32.312214704649875	20.9815904810335
8	22.636063552422446	21.649377294507367	30.6915428758277	25.023016277242487
9	22.39309755638861	19.342661683121975	34.01109994453428	24.25314081595514
10-11	25.765242382350362	26.261099631387648	25.107288508669896	22.86636947759209
12-13	25.333815837670016	24.74832998882752	24.748380688764005	25.169473484738454
14-15	25.503341453473954	24.92261252089061	25.480398243778268	24.093647781857168
16-17	26.55966257466534	23.981073059852342	24.80160371634069	24.65766064914163
18-19	26.639998105612012	23.480378440981912	25.1828403520627	24.696783101343375
20-21	26.731490603602953	23.844660423497118	24.757834764632133	24.666014208267796
22-23	26.44099491396192	24.92228744486606	23.611879148782762	25.024838492389257
24-25	25.494331777600998	23.73054979183503	24.851704194655763	25.92341423590821
26-27	25.69146705158526	23.336633280364975	24.071896235182262	26.900003432867504
28-29	25.781892835974574	24.004717121527555	23.48128209268317	26.732107949814704
30-31	25.67882463435493	24.259861230057176	24.0545592319184	26.006754903669492
32-33	24.868696119391302	23.861363000309925	24.822609879878136	26.447331000420633
34-35	25.656860842827296	24.01055357822513	24.042351381653628	26.290234197293948
36-37	26.42520453689585	23.15166879542487	23.923061935575497	26.50006473210378
38-39	26.690978376837553	23.76506153271616	23.619827108465465	25.92413298198083
40-41	26.227093135123653	24.63262615995329	24.01302904317308	25.127251661749977
42-43	25.792766353354107	24.02243665641246	24.392305015510146	25.792491974723287
44-45	27.14759387971913	24.011680906090067	23.509328101259573	25.33139711293123
46-47	26.183040028764548	24.277373778349958	24.14336309668402	25.39622309620147
48-49	26.298739856211416	24.455096532965783	24.3486177320084	24.8975458788144
50-51	25.28019772263002	24.473737547496903	24.52254071861745	25.723524011255627
52-53	25.831399826868683	24.56803211918134	24.245577579273963	25.354990474676008
54-55	26.192568237185487	23.839772353733537	24.123107423788014	25.844551985292963
56-57	24.85286432835804	24.006956865513185	24.740131541864216	26.400047264264558
58-59	25.282537291737363	24.935221891824362	24.23959796983163	25.542642846606643
60-61	25.177379671320104	24.51605276109137	24.416475115370673	25.89009245221785
62-63	25.84917462600898	24.56922505872096	24.31870775540135	25.262892559868718
64-65	26.782458945433095	24.058524659461987	23.982626863602693	25.176389531502224
66-67	26.44864718648609	23.781847506552992	23.653439004321143	26.11606630263978
68-69	26.31276882519732	23.677747305462727	23.974631186338407	26.034852683001546
70-71	25.51507302134767	24.466434879942422	24.089545598389908	25.928946500319995
72-73	25.80789295324053	24.98353296083001	23.44672561656931	25.761848469360153
74-75	26.187765518597217	25.42287929881856	22.866537344029073	25.522817838555152
76-77	26.343862810205092	24.70826721586453	23.343719567908703	25.604150406021674
78-79	25.490908041122296	24.627911719372424	24.21277472428334	25.668405515221938
80-81	26.131284085641855	24.653512160558705	24.03963732963554	25.175566424163897
82-83	25.939430815797763	24.340791534493533	23.984241697920783	25.73553595178792
84-85	25.8419649444442	24.822890750534278	22.969684794416917	26.365459510604605
86-87	26.319645999181994	24.969250217072204	22.52603748046768	26.185066303278127
88-89	26.044629483135147	24.3846763508773	22.6934800454213	26.87721412056626
90-91	25.832162462267295	24.200825323931056	23.363849346888955	26.6031628669127
92-93	25.806826429079386	24.75294743593242	23.469482856087527	25.970743278900667
94-95	25.759744881816403	25.94305560439169	22.799368419778066	25.49783109401384
96-97	26.14201230655699	25.554083459383058	22.642184180346337	25.661720053713616
98-99	25.5142719460543	25.870835737355897	22.4515387523864	26.1633535642034
100-101	25.17773125283325	26.250602438384274	22.30829635196717	26.263369956815307
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	390.0
1	279.0
2	136.0
3	111.0
4	120.5
5	136.5
6	137.0
7	132.0
8	168.5
9	189.0
10	201.0
11	235.5
12	323.0
13	403.5
14	455.5
15	683.5
16	1080.5
17	1942.5
18	2699.5
19	3430.0
20	4171.0
21	4849.0
22	6000.0
23	7211.5
24	9081.5
25	11615.0
26	15272.5
27	19910.0
28	23147.5
29	28421.5
30	39629.5
31	46242.0
32	49561.5
33	64544.0
34	82224.0
35	108231.5
36	149427.0
37	177510.5
38	219342.0
39	286339.5
40	358689.0
41	441976.0
42	507902.5
43	543569.5
44	599848.0
45	658153.5
46	712273.0
47	752570.5
48	748421.5
49	727423.0
50	678472.5
51	615764.5
52	580220.0
53	546282.0
54	487213.5
55	454859.0
56	434274.0
57	406466.5
58	399394.0
59	434461.5
60	443167.0
61	428506.5
62	464284.5
63	506291.5
64	452975.5
65	311167.5
66	206688.0
67	181310.0
68	179765.5
69	138453.0
70	95536.5
71	78441.0
72	67675.0
73	57170.5
74	50774.5
75	63226.0
76	69439.0
77	67783.5
78	68606.0
79	53968.5
80	41098.0
81	41712.5
82	42626.0
83	36499.0
84	28809.0
85	28511.0
86	33009.0
87	29440.0
88	17810.5
89	10093.5
90	7082.5
91	4253.0
92	2050.0
93	1156.0
94	721.0
95	452.0
96	326.5
97	227.0
98	115.0
99	47.5
100	14.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08445415470719925
2	0.011374678510249946
3	7.992694915435201E-4
4	0.0
5	0.004867193321638152
6	0.001980279635764542
7	0.005272792765107998
8	0.0
9	0.0
10-11	0.0
12-13	1.192939539617194E-5
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0053592808817302445
28-29	0.0
30-31	4.175288388660179E-5
32-33	5.96469769808597E-6
34-35	0.0
36-37	3.4297011763994326E-4
38-39	0.0
40-41	0.0018013387048219631
42-43	9.245281432033254E-4
44-45	0.0
46-47	0.0013569687263145583
48-49	0.0
50-51	2.9823488490429848E-5
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	3.817406526775021E-4
68-69	0.0
70-71	2.415702567724818E-4
72-73	0.0
74-75	2.9823488490429848E-5
76-77	5.6664628131816713E-5
78-79	0.0
80-81	1.192939539617194E-5
82-83	3.041995826023845E-4
84-85	5.487521882239092E-4
86-87	8.947046547128955E-4
88-89	2.773584429609976E-4
90-91	7.75410700751176E-5
92-93	6.05416816355726E-4
94-95	3.548995130361152E-4
96-97	5.96469769808597E-4
98-99	3.578818618851582E-5
100-101	6.501520490913708E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.6765309E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.19752687672363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.04323326811384	32.866387153189784
2	9.006094393324606	6.880210652866532
3	2.0828423222455656	2.3867827675185733
4	0.8388390163429115	1.2816630348801106
5	0.42367369576293434	0.8091643690432757
6	0.26261045853549897	0.6018642028811061
7	0.19017965855301375	0.5085074833289405
8	0.1359266046644911	0.41536481079469484
9	0.09957070347845712	0.342301916002738
>10	0.650570900675138	5.018716785521284
>50	0.10128739065141425	2.7211152672760646
>100	0.11631489765504323	9.758663680663146
>500	0.02355342816405842	6.333131192018918
>1k	0.022126668748075316	17.146911291365022
>5k	0.0022064020936875543	5.633884713899989
>10k+	9.701909915505559E-4	7.295330678749896
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC	93030	0.5548958268529378	TruSeq Adapter, Index 7 (97% over 35bp)
CCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGCAC	47991	0.2862518072288438	No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT	47228	0.2817007428852042	No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT	39858	0.2377409208503106	No Hit
GCCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTC	38886	0.23194323468777103	No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT	38660	0.23059521300800362	No Hit
GTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGACAGG	32597	0.19443125086450835	No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT	30152	0.17984756499268817	No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG	28494	0.16995809620926164	No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT	27775	0.16566947856433784	No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG	26418	0.15757538378803515	No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA	25596	0.1526724022802085	No Hit
CCCCCATTAAACAATACTATACGCTAGCCCTAAAGCTATTTCGAAGAGAA	24411	0.14560423550797663	No Hit
CCGGCATTCTCACTTTTAATCTCTCCACCAGTCCTCACGGTCTGACTTCA	23429	0.13974690236845622	No Hit
GCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGAC	22658	0.13514812044323193	No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG	22573	0.13464112113889462	No Hit
CGCCGGCCCATACTGCAAGATGTACGCCATCACACTTTAACGTGCTCTGA	20670	0.123290301419437	No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC	20389	0.12161422136627484	No Hit
GTCCCGTTACATTATTGGCGCAAGATCTCTTGACTAGTGAGCAATTACGC	19387	0.1156375942727927	No Hit
CGGTGTCTGATTAGTATTTAGCCTTACCGGGTGGTCCCGGCAGATTCAGA	19309	0.115172347852342	No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA	18576	0.11080022443964498	No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG	18198	0.10854556870976849	No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT	17489	0.10431659804182553	No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT	17330	0.10336821110782987	No Hit
CTCGCTAATTTGACTATGGATTCATCAAAATGCAACTGAGGTTTGCTCAG	17009	0.10145354314674428	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.771758158468776E-5	0.0	0.0	5.96469769808597E-6	0.0
2	4.771758158468776E-5	4.175288388660179E-5	0.0	2.385879079234388E-5	0.0
3	4.771758158468776E-5	5.96469769808597E-5	0.0	2.385879079234388E-5	5.96469769808597E-6
4	5.368227928277373E-5	6.561167467894568E-5	0.0	3.578818618851582E-5	1.192939539617194E-5
5	9.543516316937552E-5	7.157637237703164E-5	0.0	3.578818618851582E-5	1.192939539617194E-5
6	1.192939539617194E-4	7.754107007511762E-5	0.0	3.578818618851582E-5	1.192939539617194E-5
7	1.4315274475406328E-4	7.754107007511762E-5	0.0	3.578818618851582E-5	1.192939539617194E-5
8	2.385879079234388E-4	8.947046547128956E-5	0.0	4.771758158468776E-5	1.192939539617194E-5
9	3.5788186188515824E-4	8.947046547128956E-5	0.0	5.368227928277373E-5	1.192939539617194E-5
10-11	5.875227232614681E-4	8.947046547128956E-5	0.0	5.368227928277373E-5	1.192939539617194E-5
12-13	8.529517708262938E-4	8.947046547128956E-5	0.0	5.368227928277373E-5	2.0876441943300896E-5
14-15	0.0010975043764478187	1.0438220971650447E-4	0.0	5.96469769808597E-5	2.385879079234388E-5
16-17	0.0015269626107100084	1.1332925626363344E-4	0.0	6.561167467894568E-5	2.6841139641386866E-5
18-19	0.002379914381536302	1.1332925626363344E-4	0.0	6.859402352798867E-5	2.982348849042985E-5
20-21	0.003134448640344177	1.1332925626363344E-4	0.0	7.455872122607463E-5	2.982348849042985E-5
22-23	0.00519525169503288	1.1332925626363344E-4	0.0	7.754107007511762E-5	2.982348849042985E-5
24-25	0.010032621528180602	1.222763028107624E-4	5.96469769808597E-6	7.754107007511762E-5	2.982348849042985E-5
26-27	0.017461652511146678	1.3122334935789136E-4	5.96469769808597E-6	8.350576777320358E-5	2.982348849042985E-5
28-29	0.0295908652802045	1.3122334935789136E-4	5.96469769808597E-6	8.350576777320358E-5	2.982348849042985E-5
30-31	0.04706444718674735	1.3420569820693433E-4	5.96469769808597E-6	8.350576777320358E-5	2.982348849042985E-5
32-33	0.0696795985090403	1.371880470559773E-4	8.947046547128955E-6	9.245281432033255E-5	2.982348849042985E-5
34-35	0.09615689159084392	1.4613509360310626E-4	1.192939539617194E-5	1.0438220971650447E-4	3.578818618851582E-5
36-37	0.12750137799428568	1.5508214015023524E-4	1.192939539617194E-5	1.2525865165980538E-4	3.578818618851582E-5
38-39	0.16426180990758954	1.6402918669736416E-4	1.4911744245214925E-5	1.3122334935789136E-4	3.578818618851582E-5
40-41	0.20751481526525994	1.6701153554640717E-4	1.789409309425791E-5	1.3420569820693433E-4	3.578818618851582E-5
42-43	0.25423032763666925	1.6701153554640717E-4	1.789409309425791E-5	1.371880470559773E-4	3.578818618851582E-5
44-45	0.3042264237420259	1.7595858209353612E-4	1.789409309425791E-5	1.670115355464072E-4	3.578818618851582E-5
46-47	0.35751503297672593	1.9385267518779402E-4	1.789409309425791E-5	1.849056286406651E-4	3.578818618851582E-5
48-49	0.422876190352352	2.02799721734923E-4	1.789409309425791E-5	1.9087032633875105E-4	3.578818618851582E-5
50-51	0.49441080984549707	2.0578207058396598E-4	1.789409309425791E-5	1.9385267518779402E-4	3.578818618851582E-5
52-53	0.5709378813119401	2.1472911713109493E-4	1.789409309425791E-5	1.9981737288588003E-4	3.578818618851582E-5
54-55	0.6593048777090836	2.2367616367822388E-4	1.789409309425791E-5	2.0578207058396598E-4	3.578818618851582E-5
56-57	0.763329205563703	2.475349544705678E-4	1.789409309425791E-5	2.1174676828205192E-4	3.578818618851582E-5
58-59	0.8825873713392339	2.8630548950812657E-4	1.789409309425791E-5	2.206938148291809E-4	3.578818618851582E-5
60-61	1.016429222986585	3.0419958260238453E-4	1.789409309425791E-5	2.2964086137630986E-4	3.578818618851582E-5
62-63	1.1726416733506075	3.429701176399433E-4	1.789409309425791E-5	2.385879079234388E-4	3.578818618851582E-5
64-65	1.3538670835115534	3.638465595832442E-4	1.789409309425791E-5	2.564820010176967E-4	3.578818618851582E-5
66-67	1.5596670481886137	3.7577595497941614E-4	1.789409309425791E-5	2.564820010176967E-4	3.578818618851582E-5
68-69	1.802713567641372	3.787583038284591E-4	1.789409309425791E-5	2.564820010176967E-4	3.578818618851582E-5
70-71	2.06888223772076	3.90687699224631E-4	1.789409309425791E-5	2.564820010176967E-4	3.578818618851582E-5
72-73	2.360072814643619	3.96652396922717E-4	1.789409309425791E-5	2.624466987157827E-4	3.578818618851582E-5
74-75	2.6827361189704284	4.145464900169749E-4	1.789409309425791E-5	2.6841139641386867E-4	3.578818618851582E-5
76-77	3.053799962768357	4.443699785074048E-4	1.789409309425791E-5	2.7437609411195467E-4	3.578818618851582E-5
78-79	3.4785729269887002	4.4735232735644776E-4	1.789409309425791E-5	2.803407918100406E-4	3.578818618851582E-5
80-81	3.9487730288776666	4.533170250545337E-4	1.789409309425791E-5	2.803407918100406E-4	3.578818618851582E-5
82-83	4.490084256723213	4.6524642045070566E-4	1.789409309425791E-5	2.8630548950812657E-4	3.578818618851582E-5
84-85	5.113466742545574	4.6524642045070566E-4	2.0876441943300896E-5	2.8928783835716954E-4	3.578818618851582E-5
86-87	5.774459629703216	4.7121111814879167E-4	2.982348849042985E-5	2.9823488490429847E-4	4.175288388660179E-5
88-89	6.473912887618116	4.7121111814879167E-4	3.578818618851582E-5	3.101642803004705E-4	4.473523273564477E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCGAT	7695	0.0	37.572212	1
GGCGATC	7980	0.0	35.30562	2
CGGCATT	14990	0.0	32.704823	3
GGCGCGT	8810	0.0	31.543776	5
GCGATCT	10620	0.0	27.688528	3
TAAAACG	13755	0.0	27.228212	58-59
GCATTCT	18155	0.0	26.846325	5
CGCTAAT	6495	0.0	26.842358	3
ACGCTCT	13855	0.0	26.654583	62-63
GCGTGCC	10515	0.0	26.651447	8
CGAAATT	14180	0.0	26.345142	52-53
GGCATTC	19285	0.0	25.889091	4
CCCGTTA	17935	0.0	25.665266	1
TCAACGA	14770	0.0	25.485714	48-49
AACGAAA	14890	0.0	25.248423	50-51
AACGCAC	14645	0.0	24.973572	72-73
GCCGTCT	21145	0.0	24.855457	48-49
CTTGAAA	21015	0.0	24.8397	60-61
TCGCTAA	7105	0.0	24.808578	2
GCTTGAA	21825	0.0	24.385723	58-59
>>END_MODULE