FastQCFastQC Report
Thu 26 May 2016
SRR2048276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5833619
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT494020.8468499571192427No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG104410.17897980653176013No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC102210.17520856264353224No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA89160.15283822957927146No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC76990.13197639407030182No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG73100.125308149195208No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA68730.11781708747177352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG213300.093.111221
TCGACGG224800.088.0347062
TATCTCG223850.087.4106752
CGACGGC238550.082.920563
ATATCTC239300.082.379151
ATCTCGA239800.081.457963
GACGGCG241800.081.394764
ACGGCGC242100.081.115185
TCTCGAG228000.080.902764
CGGCGCG245250.079.90076
CTCGAGG238450.076.757445
TCGAGGG239050.076.370396
AGGGCGC247350.073.9754649
CGAGGGC268700.067.9917
GAGGGCG270150.067.5035868
CTCGACG15600.064.26725
TCTCGAC18850.053.4400864
CGCGCCG492000.042.2196169
GCGCGCC493300.041.867688
GGCGCGC491100.041.8518037
Produced by FastQC (version 0.11.5)