##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048276_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5833619 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.85582483189252 31.0 31.0 34.0 28.0 34.0 2 31.07872968735188 31.0 31.0 34.0 28.0 34.0 3 31.122819985329862 33.0 31.0 34.0 28.0 34.0 4 34.57175314328892 37.0 35.0 37.0 32.0 37.0 5 34.57739081006147 37.0 35.0 37.0 32.0 37.0 6 34.58751814268295 37.0 35.0 37.0 32.0 37.0 7 34.475023137438356 37.0 35.0 37.0 31.0 37.0 8 34.29548998657609 37.0 35.0 37.0 30.0 37.0 9 35.90120506670045 39.0 37.0 39.0 30.0 39.0 10-11 35.64285411851545 39.0 37.0 39.0 30.0 39.0 12-13 35.71694003327951 39.0 37.0 39.0 30.0 39.0 14-15 36.944722821288124 40.0 37.0 41.0 30.5 41.0 16-17 36.921595496723384 40.0 37.0 41.0 30.0 41.0 18-19 36.914553470152924 40.0 37.0 41.0 30.5 41.0 20-21 36.9429964486882 40.0 37.5 41.0 30.0 41.0 22-23 36.98107684097984 40.0 37.5 41.0 30.5 41.0 24-25 37.051078841453304 40.0 38.0 41.0 31.0 41.0 26-27 37.001283851413675 40.0 37.5 41.0 30.5 41.0 28-29 36.87615963606811 40.0 37.0 41.0 30.0 41.0 30-31 36.661408124185 40.0 36.5 41.0 30.0 41.0 32-33 36.41137654001744 40.0 35.5 41.0 30.0 41.0 34-35 36.261244949318765 40.0 35.0 41.0 30.0 41.0 36-37 36.16805074174368 40.0 35.0 41.0 30.0 41.0 38-39 36.04047316082864 40.0 35.0 41.0 29.5 41.0 40-41 35.5969766794849 39.5 35.0 41.0 26.5 41.0 42-43 35.37805237194955 39.0 35.0 41.0 26.0 41.0 44-45 35.14974992367517 39.0 35.0 41.0 24.5 41.0 46-47 34.81683839825672 39.0 35.0 41.0 23.0 41.0 48-49 34.31076455284447 39.0 34.0 41.0 18.5 41.0 50-51 33.980392445924224 39.0 34.0 41.0 13.5 41.0 52-53 33.77531563168592 39.0 33.5 40.5 9.0 41.0 54-55 33.564298765483315 38.5 33.0 40.0 7.5 41.0 56-57 33.337036580551455 38.0 33.0 40.0 6.5 41.0 58-59 33.11443531022509 38.0 33.0 40.0 2.0 41.0 60-61 32.86412996803528 37.0 33.0 40.0 2.0 41.0 62-63 32.575005069751725 37.0 32.0 40.0 2.0 41.0 64-65 32.275880375458186 36.5 32.0 40.0 2.0 41.0 66-67 31.963160518367758 36.0 32.0 39.0 2.0 41.0 68-69 31.615601567397526 35.0 31.5 39.0 2.0 41.0 70-71 31.295014209875553 35.0 31.0 39.0 2.0 40.5 72-73 30.955941671884982 35.0 31.0 37.5 2.0 40.0 74-75 30.614236034269638 35.0 31.0 37.0 2.0 39.5 76-77 30.306260059150247 35.0 31.0 36.5 2.0 39.0 78-79 30.007463805915332 35.0 30.5 36.0 2.0 39.0 80-81 29.702827438679144 35.0 30.0 36.0 2.0 37.5 82-83 29.470191231206563 35.0 30.0 35.0 2.0 37.0 84-85 29.244806954310867 34.0 30.0 35.0 2.0 37.0 86-87 29.003561682722165 34.0 30.0 35.0 2.0 36.0 88-89 28.796861862250516 34.0 30.0 35.0 2.0 36.0 90-91 28.609542892670913 34.0 29.0 35.0 2.0 36.0 92-93 28.412330407590897 34.0 29.0 35.0 2.0 35.5 94-95 28.214115543027408 34.0 29.0 35.0 2.0 35.0 96-97 28.006270978615504 34.0 29.0 35.0 2.0 35.0 98-99 27.72804351809743 34.0 29.0 35.0 2.0 35.0 100-101 26.4788318366352 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7324083862120006 1101 2 0.7757792454641574 1101 3 0.7341335520678705 1101 4 0.7382438983471076 1101 5 0.751109743194867 1101 6 0.7432074779450204 1101 7 0.7877164799910688 1101 8 0.949838744449842 1101 9 0.9838807629396271 1101 10-11 1.0221496550549887 1101 12-13 1.029036545454943 1101 14-15 1.1410970863583003 1101 16-17 1.082132682856539 1101 18-19 1.1061522264875805 1101 20-21 1.1069427506069331 1101 22-23 1.0904981345610167 1101 24-25 1.067484117022218 1101 26-27 1.1314127441994515 1101 28-29 1.1437109033634485 1101 30-31 1.0338319518623749 1101 32-33 1.0778849282043055 1101 34-35 1.0530116456659826 1101 36-37 0.9708666489415236 1101 38-39 0.989517782802217 1101 40-41 0.9894266544514991 1101 42-43 0.8688251934725955 1101 44-45 0.7677218828504024 1101 46-47 0.7937638963413889 1101 48-49 0.5934453636959347 1101 50-51 0.5170175295288786 1101 52-53 0.48106007703162135 1101 54-55 0.4419234573942319 1101 56-57 0.4301083453425676 1101 58-59 0.46396833911585134 1101 60-61 0.47634514508781933 1101 62-63 0.401793913581173 1101 64-65 0.4413322271784139 1101 66-67 0.4484168866826863 1101 68-69 0.4616750687407709 1101 70-71 0.47935236916330837 1101 72-73 0.48600825555068994 1101 74-75 0.45931387584259653 1101 76-77 0.5649107470776507 1101 78-79 0.4862744769014995 1101 80-81 0.5548656053472385 1101 82-83 0.4662761111830278 1101 84-85 0.5197886143152388 1101 86-87 0.5354404515573243 1101 88-89 0.5945451891433677 1101 90-91 0.5837975918905762 1101 92-93 0.5890938104604153 1101 94-95 0.637220044924252 1101 96-97 0.6756138639749203 1101 98-99 0.6685158201550223 1101 100-101 0.7699304869340438 1102 1 0.7103911076519118 1102 2 0.7651465806292137 1102 3 0.7206213470071674 1102 4 0.7844984765308425 1102 5 0.7643302713454645 1102 6 0.7990163346876855 1102 7 0.8763933615374953 1102 8 1.0197870333392203 1102 9 0.9868922780065787 1102 10-11 1.1266569154211155 1102 12-13 1.0713695384027417 1102 14-15 1.1813842663854572 1102 16-17 1.1325218556310972 1102 18-19 1.163037339243644 1102 20-21 1.141186874340903 1102 22-23 1.1272339554696345 1102 24-25 1.1296774807232879 1102 26-27 1.16690846484844 1102 28-29 1.1762854929451052 1102 30-31 1.1545822533494814 1102 32-33 1.1827979866921723 1102 34-35 1.0909355867356894 1102 36-37 1.0957974893739078 1102 38-39 1.0816324432746214 1102 40-41 1.0803215282399563 1102 42-43 0.9786526325636942 1102 44-45 0.9030102207429209 1102 46-47 0.7547972762547701 1102 48-49 0.5715077840086806 1102 50-51 0.4667956415437402 1102 52-53 0.42487043584612394 1102 54-55 0.40970149390311406 1102 56-57 0.4257460916146556 1102 58-59 0.3750102798107875 1102 60-61 0.2891429581264049 1102 62-63 0.2793045316300393 1102 64-65 0.29215545617740446 1102 66-67 0.31668439667637216 1102 68-69 0.38078000272147605 1102 70-71 0.399853766370434 1102 72-73 0.3663851334976478 1102 74-75 0.2931908403610599 1102 76-77 0.2534239077978313 1102 78-79 0.31798504041902476 1102 80-81 0.33800755909933855 1102 82-83 0.31200655853800896 1102 84-85 0.3309737931922925 1102 86-87 0.32088225204717347 1102 88-89 0.39454006467829217 1102 90-91 0.362658563124306 1102 92-93 0.3902355619051967 1102 94-95 0.41984982267851834 1102 96-97 0.4689958295131156 1102 98-99 0.472123401299811 1102 100-101 0.5157918051131603 1103 1 0.587902937938523 1103 2 0.6447608631169288 1103 3 0.6044861081899064 1103 4 0.6162972795458614 1103 5 0.6822414109691195 1103 6 0.6652898855293756 1103 7 0.7011243834935357 1103 8 0.8564164378182326 1103 9 0.8622866094947099 1103 10-11 0.9977553628938836 1103 12-13 0.9800469191586529 1103 14-15 1.0100210309840207 1103 16-17 1.004112198097289 1103 18-19 1.0493088962100217 1103 20-21 1.0479917422611464 1103 22-23 1.0476608620364587 1103 24-25 1.0133461775004804 1103 26-27 1.0431211864005263 1103 28-29 1.0376482849949369 1103 30-31 1.0537787811419506 1103 32-33 1.1248974891164778 1103 34-35 1.0646474752936683 1103 36-37 1.040604116056322 1103 38-39 1.0271535419433704 1103 40-41 1.0211305878042864 1103 42-43 0.8919984414305517 1103 44-45 0.8864495977204498 1103 46-47 0.7593779996002326 1103 48-49 0.6258808441945121 1103 50-51 0.6113819924775399 1103 52-53 0.5451200259068045 1103 54-55 0.5594316408230853 1103 56-57 0.5948873549446745 1103 58-59 0.5642570640431472 1103 60-61 0.5629548059781087 1103 62-63 0.6015848008176903 1103 64-65 0.5559096755611321 1103 66-67 0.5960500643160245 1103 68-69 0.5601524533994144 1103 70-71 0.5694323304889366 1103 72-73 0.5800083227663784 1103 74-75 0.6029201874779346 1103 76-77 0.533626077742472 1103 78-79 0.5466735991070735 1103 80-81 0.5410568526374533 1103 82-83 0.5585476475367024 1103 84-85 0.5193467161080925 1103 86-87 0.5228890738686651 1103 88-89 0.60115986749706 1103 90-91 0.5675463255657291 1103 92-93 0.6260992957352336 1103 94-95 0.6011699628548719 1103 96-97 0.6555887962829097 1103 98-99 0.7875060769050677 1103 100-101 0.784623543037803 1104 1 0.42758248839712465 1104 2 0.5082904428492085 1104 3 0.4950101203298267 1104 4 0.584103586358502 1104 5 0.5422324717097382 1104 6 0.5520534421186696 1104 7 0.5126814927352328 1104 8 0.6664458819699703 1104 9 0.6713685347703802 1104 10-11 0.8058717096455226 1104 12-13 0.7212134339076357 1104 14-15 0.8174960205024959 1104 16-17 0.7305160427246449 1104 18-19 0.7989580116335517 1104 20-21 0.8135810807825834 1104 22-23 0.7825842257337996 1104 24-25 0.7620017459185675 1104 26-27 0.7889178965612942 1104 28-29 0.8284410076493529 1104 30-31 0.8286138603300444 1104 32-33 0.8687270294797926 1104 34-35 0.7728735650389282 1104 36-37 0.7911396458832414 1104 38-39 0.8507688490665899 1104 40-41 0.8323433384484886 1104 42-43 0.7258554335242593 1104 44-45 0.6564870435215298 1104 46-47 0.6798537514595964 1104 48-49 0.5046672038862283 1104 50-51 0.45828604140800167 1104 52-53 0.4287921862935278 1104 54-55 0.3610953126425187 1104 56-57 0.3372517197964413 1104 58-59 0.3553773109329228 1104 60-61 0.38219285930787805 1104 62-63 0.3061836182570232 1104 64-65 0.23666810619371148 1104 66-67 0.28707452581620885 1104 68-69 0.2885807366194477 1104 70-71 0.29405610114099545 1104 72-73 0.26230837609294966 1104 74-75 0.23248553596787502 1104 76-77 0.2744860046621618 1104 78-79 0.25680729910275346 1104 80-81 0.27588997136818705 1104 82-83 0.2794163965145202 1104 84-85 0.3345117594485849 1104 86-87 0.35567090426459913 1104 88-89 0.40169201446956393 1104 90-91 0.4153933118686304 1104 92-93 0.4801585345770043 1104 94-95 0.5286928131041648 1104 96-97 0.5224434821755573 1104 98-99 0.5604864462275323 1104 100-101 0.5276294782159212 1105 1 0.3937471380888091 1105 2 0.4453791256313444 1105 3 0.4250228415166255 1105 4 0.47150775434216996 1105 5 0.4411733201261896 1105 6 0.46530613111057306 1105 7 0.45539905373777856 1105 8 0.6178830416292698 1105 9 0.5441453018849245 1105 10-11 0.7058445768322414 1105 12-13 0.6557725945022383 1105 14-15 0.6966812378168541 1105 16-17 0.6399388663117094 1105 18-19 0.6522137095098941 1105 20-21 0.60651427754064 1105 22-23 0.572229541855549 1105 24-25 0.6558712680621994 1105 26-27 0.6708030807888932 1105 28-29 0.714492210151839 1105 30-31 0.6672115211866654 1105 32-33 0.6757804807609418 1105 34-35 0.6299542834327596 1105 36-37 0.6557913419490617 1105 38-39 0.5820378719283426 1105 40-41 0.6732884735866165 1105 42-43 0.5192803105348816 1105 44-45 0.46924582260182746 1105 46-47 0.36312549335011113 1105 48-49 0.2996086822680084 1105 50-51 0.3078095153276905 1105 52-53 0.2919961852505608 1105 54-55 0.2765655304051151 1105 56-57 0.2188367015178656 1105 58-59 0.15265719477577022 1105 60-61 0.12297840342323951 1105 62-63 0.1987059656790251 1105 64-65 0.22209510380647401 1105 66-67 0.15075890510224355 1105 68-69 0.2041967125654658 1105 70-71 0.15789879488334435 1105 72-73 0.11436963955639357 1105 74-75 0.12015973241262046 1105 76-77 0.14991400822083634 1105 78-79 0.10006633366963413 1105 80-81 0.1823055278841501 1105 82-83 0.22605895889183358 1105 84-85 0.2562911541527626 1105 86-87 0.230708847458434 1105 88-89 0.20149316036412657 1105 90-91 0.21072681456363185 1105 92-93 0.22549456874370222 1105 94-95 0.2245835153913056 1105 96-97 0.2770976282669757 1105 98-99 0.32828658403894195 1105 100-101 0.30498873041138097 1106 1 0.2658982387351472 1106 2 0.3399876422959025 1106 3 0.3011715447546415 1106 4 0.37029213059425103 1106 5 0.38247124852289716 1106 6 0.42183086435112216 1106 7 0.4443817993831658 1106 8 0.5616615759284187 1106 9 0.46019869529185087 1106 10-11 0.6305572321563631 1106 12-13 0.547166966194105 1106 14-15 0.553656275906512 1106 16-17 0.5648794500588963 1106 18-19 0.5718935289727938 1106 20-21 0.5728226344658367 1106 22-23 0.4933443136602307 1106 24-25 0.5079583658801567 1106 26-27 0.5527597989359165 1106 28-29 0.5990323779440629 1106 30-31 0.6282554893784962 1106 32-33 0.6302544348108299 1106 34-35 0.5735149389176755 1106 36-37 0.6279036774649711 1106 38-39 0.6955822651692003 1106 40-41 0.7176200988159493 1106 42-43 0.5719217266532723 1106 44-45 0.5034698478227213 1106 46-47 0.5072220153946674 1106 48-49 0.4300098527673697 1106 50-51 0.3628145664917284 1106 52-53 0.3504131197969613 1106 54-55 0.374001595123282 1106 56-57 0.38146420798915415 1106 58-59 0.31305377046217586 1106 60-61 0.3310847968196242 1106 62-63 0.30653398491101314 1106 64-65 0.320209187624819 1106 66-67 0.2911380191814388 1106 68-69 0.3346059145144942 1106 70-71 0.3580725048430651 1106 72-73 0.3469425881841133 1106 74-75 0.35205709196851487 1106 76-77 0.3701050150400711 1106 78-79 0.33282206985455076 1106 80-81 0.4031902872075541 1106 82-83 0.418672850614378 1106 84-85 0.4124005008161582 1106 86-87 0.3849674186215246 1106 88-89 0.36697772119951466 1106 90-91 0.3870686185639691 1106 92-93 0.33670844122372046 1106 94-95 0.41469530163843515 1106 96-97 0.3847246965094193 1106 98-99 0.4156120421034082 1106 100-101 0.5127355207969728 1107 1 0.20158389889195405 1107 2 0.26290749887687426 1107 3 0.2662428910744872 1107 4 0.28717787063761335 1107 5 0.2721064795768555 1107 6 0.24640916506331934 1107 7 0.27422975471694144 1107 8 0.403825853262461 1107 9 0.34593690272644295 1107 10-11 0.4840611210380672 1107 12-13 0.35022008037263674 1107 14-15 0.4051094799334791 1107 16-17 0.34625080549449194 1107 18-19 0.45491708640514616 1107 20-21 0.42241233784950794 1107 22-23 0.375679480201498 1107 24-25 0.3492455514805215 1107 26-27 0.42294247955112496 1107 28-29 0.48700306641318036 1107 30-31 0.423440999708113 1107 32-33 0.4050419747441296 1107 34-35 0.38788515464488427 1107 36-37 0.35303036804971555 1107 38-39 0.39988042175992433 1107 40-41 0.5043068472753305 1107 42-43 0.3592560366970545 1107 44-45 0.32056050069730446 1107 46-47 0.2618625873849183 1107 48-49 0.0966965549295864 1107 50-51 0.18095508731563115 1107 52-53 0.21387872092648053 1107 54-55 0.16485341179993185 1107 56-57 0.20734381812874147 1107 58-59 0.1548772049037126 1107 60-61 0.12592790771425655 1107 62-63 0.04086247572094237 1107 64-65 0.12211085947916445 1107 66-67 0.15054212203585138 1107 68-69 0.14039279622987877 1107 70-71 0.2155160027500358 1107 72-73 0.16610555786282433 1107 74-75 0.16223422171375645 1107 76-77 0.22901673162226288 1107 78-79 0.16372336542472965 1107 80-81 0.2104639862852622 1107 82-83 0.2077981423862738 1107 84-85 0.24340804038865826 1107 86-87 0.21707982397775183 1107 88-89 0.1814804825058829 1107 90-91 0.22355147592257296 1107 92-93 0.12241560561360387 1107 94-95 0.20310386546378112 1107 96-97 0.17224016415145726 1107 98-99 0.18849022325717613 1107 100-101 0.22623639557384934 1108 1 0.20452546681273276 1108 2 0.23693464986220292 1108 3 0.3085183014198769 1108 4 0.25188642103265124 1108 5 0.2221170538827053 1108 6 0.2440326503442236 1108 7 0.28012605049588046 1108 8 0.5012507754334052 1108 9 0.36775322028649526 1108 10-11 0.46029386207635525 1108 12-13 0.4236004577780079 1108 14-15 0.40380284752075823 1108 16-17 0.3110786838148769 1108 18-19 0.364750629421863 1108 20-21 0.370575576288104 1108 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2215 56-57 -0.7580056761785912 2215 58-59 -0.7954972174610049 2215 60-61 -0.7360294451900131 2215 62-63 -0.6647859449527118 2215 64-65 -0.6809829489670278 2215 66-67 -0.7389492195767566 2215 68-69 -0.7411488912480912 2215 70-71 -0.6268020268937491 2215 72-73 -0.7057933917096584 2215 74-75 -0.7157697424204521 2215 76-77 -0.7520535620796274 2215 78-79 -0.7629250071752551 2215 80-81 -0.6903501202283309 2215 82-83 -0.7650601907614814 2215 84-85 -0.7800317544887392 2215 86-87 -0.8066814083838914 2215 88-89 -0.8871799184109115 2215 90-91 -0.954250554465645 2215 92-93 -0.8810224686856785 2215 94-95 -0.8355059250940506 2215 96-97 -0.9648220317869551 2215 98-99 -1.1133774744484732 2215 100-101 -1.221501342457131 2216 1 -1.061865505197332 2216 2 -1.0896792772860486 2216 3 -1.183658495701028 2216 4 -1.0967358122121453 2216 5 -1.102768599183051 2216 6 -1.1882153255579482 2216 7 -1.2573474953927217 2216 8 -1.2098312118045342 2216 9 -1.4138521880558343 2216 10-11 -1.2911964178396715 2216 12-13 -1.4158215526935578 2216 14-15 -1.721149628749295 2216 16-17 -1.5606351691729898 2216 18-19 -1.52507123544936 2216 20-21 -1.5182933939874133 2216 22-23 -1.4622385228629042 2216 24-25 -1.5261206429229972 2216 26-27 -1.7476717110924724 2216 28-29 -1.6501788621853137 2216 30-31 -1.5498302354957971 2216 32-33 -1.5855093303308436 2216 34-35 -1.5247704855706914 2216 36-37 -1.5055417347996567 2216 38-39 -1.7261227265473593 2216 40-41 -1.5377275592562114 2216 42-43 -1.5257460879392681 2216 44-45 -1.3509735378865315 2216 46-47 -1.27999136255211 2216 48-49 -1.3098292100002453 2216 50-51 -1.275655871970777 2216 52-53 -1.216896185334356 2216 54-55 -1.2145345207006315 2216 56-57 -1.1981122104260962 2216 58-59 -1.1572238449058005 2216 60-61 -1.150059898958034 2216 62-63 -1.1187546702284585 2216 64-65 -1.070470422941682 2216 66-67 -1.0606400028468883 2216 68-69 -1.0878129798392067 2216 70-71 -1.0855042703349014 2216 72-73 -1.0536312605135763 2216 74-75 -1.0612065665883534 2216 76-77 -1.0953254166389392 2216 78-79 -1.1277774945552395 2216 80-81 -1.113018448469937 2216 82-83 -1.138952950382805 2216 84-85 -1.211774081253946 2216 86-87 -1.1874016686215718 2216 88-89 -1.2117144205497787 2216 90-91 -1.304390955663262 2216 92-93 -1.322161283092587 2216 94-95 -1.3226133042793329 2216 96-97 -1.4326031774403951 2216 98-99 -1.4356961446310947 2216 100-101 -1.539125831363915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119149.0 3 24344.0 4 14566.0 5 10199.0 6 9106.0 7 9634.0 8 11856.0 9 13458.0 10 14548.0 11 16042.0 12 17911.0 13 22782.0 14 31109.0 15 48267.0 16 51822.0 17 73182.0 18 111502.0 19 53032.0 20 34044.0 21 29949.0 22 31509.0 23 34755.0 24 40085.0 25 45986.0 26 54083.0 27 64412.0 28 77370.0 29 89706.0 30 102810.0 31 124106.0 32 156030.0 33 201644.0 34 276060.0 35 417030.0 36 717279.0 37 1192779.0 38 1216564.0 39 274799.0 40 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.126651060032497 37.801309667768 16.62379186337862 19.448247408820883 2 22.734394577047023 27.88302341085726 32.582123581705254 16.800458430390464 3 21.68581739430628 29.94683780187456 23.040982688629423 25.32636211518974 4 25.222214122459828 25.862154431975576 27.343233148801687 21.572398296762906 5 22.04208353622591 29.280918984587483 24.37607853233329 24.300918946853322 6 22.147055308831373 24.972101012764526 27.974767554159353 24.90607612424475 7 25.576712806434127 25.704156899210133 23.85524037971962 24.86388991463612 8 28.61798037520749 25.067780180653426 25.17839551531282 21.13584392882627 9 21.18344382100483 28.307815738450227 25.34588898431915 25.1628514562258 10-11 26.807747895096874 25.48851239796942 24.866350866906345 22.837388840027362 12-13 25.06167272864428 25.529157926589352 24.74381536842051 24.66535397634586 14-15 24.882232935380248 25.513138486593036 24.98216767520061 24.62246090282611 16-17 24.914078948270777 27.231581365684388 24.90663811489744 22.947701571147398 18-19 23.008972153072417 25.293480300585685 26.900214168888326 24.79733337745357 20-21 22.836380363764547 28.829242165195275 25.41912956291607 22.915247908124112 22-23 21.195051897501877 27.17271174520245 28.957405265498696 22.674831091796975 24-25 21.15343335314161 25.22388899042585 30.972633370698173 22.65004428573437 26-27 21.099440455425874 25.181443103139095 32.926784317665245 20.792332123769793 28-29 21.15494108592812 25.21058864010306 32.93602388005658 20.69844639391224 30-31 21.260128353406536 25.004965120723366 32.854175968678554 20.880730557191548 32-33 21.184517143548803 24.95899874295958 32.99172191221017 20.864762201281454 34-35 21.320465354581323 25.11012264251139 32.824755180866696 20.744656822040593 36-37 21.304561895219525 25.03117859326268 32.70106538326972 20.963194128248077 38-39 21.39249957215961 25.00437271739865 32.637765462528904 20.965362247912843 40-41 21.93756411280905 25.593576149469143 31.14693292552642 21.321926812195382 42-43 22.261340455328803 25.611677072678773 30.610404205768347 21.51657826622408 44-45 22.370824306858474 25.93037211798012 30.138368507218694 21.560435067942713 46-47 22.58056384574598 26.402515020087375 29.344481819846752 21.672439314319895 48-49 22.814749162267848 26.764939878279947 28.203366157764787 22.21694480168742 50-51 23.102432874332205 26.870168018987567 27.506199417652162 22.52119968902807 52-53 23.177775769437677 27.0253200048909 27.144462780551443 22.652441445119983 54-55 23.312027450269454 26.72112596049273 27.062401991194196 22.90444459804362 56-57 23.254521703025457 26.58795336438784 27.378879448064193 22.77864548452251 58-59 23.310784071011525 26.833936589771874 27.226752874497134 22.628526464719467 60-61 23.328413387922662 26.781359249543108 27.117520265189977 22.77270709734426 62-63 23.334385558970645 26.840603766556153 27.19910656009945 22.625904114373753 64-65 23.40779932112183 27.004509018164963 26.985094368147898 22.602597292565314 66-67 23.412237805979665 26.91290262918779 26.901507129818402 22.773352435014143 68-69 23.45070287891377 26.90481981828659 26.9986678991122 22.64580940368744 70-71 23.49119796358202 27.08905033545223 26.848000707794473 22.571750993171275 72-73 23.50384589697401 26.9877140659169 26.734593113899543 22.773846923209547 74-75 23.472511561223428 27.006853311828177 26.859814132188482 22.660820994759916 76-77 23.54266846630871 27.191273239315382 26.670096652026213 22.595961642349693 78-79 23.548737749135004 27.13492538060786 26.6017116957531 22.714625174504036 80-81 23.443777179362723 27.071933530829163 26.783946643029587 22.700342646778527 82-83 23.45514536501805 27.23497970275876 26.65830120250189 22.651573729721303 84-85 23.471226622882543 27.04445936729861 26.69559215456525 22.788721855253595 86-87 23.452395693592972 26.903553125376238 26.886908622795612 22.757142558235177 88-89 23.48790782471191 27.044195910218967 26.664339874078784 22.80355639099034 90-91 23.52123728125962 26.95611931132232 26.607503266855186 22.91514014056288 92-93 23.53715234718676 26.940235102375947 26.75139454686026 22.77121800357703 94-95 23.598458155426485 27.12417109820801 26.593183518756003 22.684187227609502 96-97 23.55930941989632 27.122943582344416 26.42006145524329 22.897685542515976 98-99 23.540040742430968 27.065561603315224 26.613384234080108 22.7810134201737 100-101 23.782623169878438 27.123252226461602 26.20693007688416 22.887194526775794 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 337.0 1 277.0 2 228.0 3 231.0 4 250.5 5 287.5 6 302.5 7 309.5 8 299.0 9 294.0 10 307.5 11 333.0 12 359.0 13 401.0 14 467.5 15 524.5 16 938.5 17 1121.5 18 1135.5 19 1726.0 20 2767.0 21 4329.0 22 6762.0 23 10214.0 24 14487.5 25 19883.0 26 26749.5 27 35290.5 28 45167.5 29 56082.5 30 68075.0 31 81627.5 32 97243.5 33 112833.0 34 124077.5 35 135967.0 36 150193.5 37 166180.0 38 179039.5 39 186504.5 40 192999.5 41 201094.5 42 214334.0 43 226131.5 44 231771.0 45 232423.5 46 231589.0 47 230185.0 48 229918.0 49 225110.0 50 216566.0 51 208520.0 52 190979.0 53 169117.5 54 153852.0 55 141844.0 56 129042.5 57 116024.5 58 106258.5 59 98168.0 60 87276.5 61 76592.5 62 68030.0 63 60108.5 64 52374.0 65 44482.5 66 37111.5 67 30857.5 68 25074.0 69 19713.0 70 15183.0 71 11363.0 72 8196.0 73 5884.5 74 4084.5 75 2680.5 76 1709.0 77 1117.0 78 781.5 79 507.5 80 298.5 81 174.5 82 131.5 83 109.0 84 82.5 85 64.5 86 48.0 87 37.5 88 29.0 89 22.5 90 21.5 91 17.5 92 10.5 93 10.0 94 16.0 95 13.5 96 6.5 97 5.0 98 4.5 99 4.5 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03275839577456121 2 0.05639723814668047 3 0.04450067788108891 4 0.06075131063581629 5 0.05756289534849636 6 0.016627757143550168 7 0.008108174359689928 8 0.013593620015294109 9 0.006668244875093832 10-11 0.013867892298074317 12-13 0.013953602386443132 14-15 0.013927889359932489 16-17 0.016096354595663515 18-19 0.018384813955110883 20-21 0.01808482864582003 22-23 0.019901882519238915 24-25 0.017844840398387348 26-27 0.011330873682357384 28-29 0.013747898174357976 30-31 0.007328212555533709 32-33 0.014930697393847626 34-35 0.011836563203733394 36-37 0.02558446137809137 38-39 0.03436974543589494 40-41 0.03601537913257619 42-43 0.03933235955244934 44-45 0.037061042210675736 46-47 0.035912527026533615 48-49 0.04624916368381274 50-51 0.03813241831528593 52-53 0.040798002063556084 54-55 0.053963071637006116 56-57 0.04792908141584152 58-59 0.049771848315771056 60-61 0.05024325380179953 62-63 0.05349166615097764 64-65 0.05092036349991318 66-67 0.04061801087798157 68-69 0.03177272975831983 70-71 0.02429881005255914 72-73 0.027847207711028093 74-75 0.03154131251972404 76-77 0.029458557372361825 78-79 0.035861100973512326 80-81 0.0360582341767606 82-83 0.04972042226274976 84-85 0.04954900208601212 86-87 0.04911188063533117 88-89 0.05123749082687779 90-91 0.04637772881636596 92-93 0.03847525866876119 94-95 0.03484115092192342 96-97 0.035278272372604384 98-99 0.027692929551964226 100-101 0.028515746400304853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5833619.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.393640474273795 #Duplication Level Percentage of deduplicated Percentage of total 1 80.87250021399845 44.79822201109861 2 11.334299886837217 12.55696265918126 3 3.113124398838004 5.173418811027664 4 1.262968948978225 2.79841791559485 5 0.6928836186438571 1.919067303083583 6 0.4404501533790046 1.4638882465869199 7 0.3011623341543538 1.1677734643777566 8 0.23199403807750807 1.0280795469952373 9 0.1873162815987493 0.9338517682073053 >10 1.3382441476354459 14.100646667427172 >50 0.16072164044175594 6.278068642180306 >100 0.06289036495955878 5.12327874725706 >500 6.905973290093855E-4 0.28584915695878127 >1k 5.336407163114518E-4 0.6186449552078644 >5k 1.2556252147237747E-4 0.535528341378423 >10k+ 9.41718911042831E-5 1.2183017634371658 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 49402 0.8468499571192427 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 10441 0.17897980653176013 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 10221 0.17520856264353224 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 8916 0.15283822957927146 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 7699 0.13197639407030182 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 7310 0.125308149195208 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 6873 0.11781708747177352 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7142017673763064E-5 0.0 0.0 0.0 0.0 2 1.7142017673763064E-5 0.0 0.0 0.0 0.0 3 1.7142017673763064E-5 0.0 0.0 0.0 0.0 4 1.7142017673763064E-5 1.7142017673763064E-5 0.0 0.0 0.0 5 6.856807069505225E-5 3.428403534752613E-5 0.0 0.0 0.0 6 6.856807069505225E-5 5.142605302128919E-5 0.0 0.0 0.0 7 8.571008836881531E-5 5.142605302128919E-5 0.0 0.0 0.0 8 1.0285210604257837E-4 5.142605302128919E-5 0.0 0.0 0.0 9 1.1999412371634143E-4 6.856807069505225E-5 0.0 0.0 0.0 10-11 1.971332032482752E-4 6.856807069505225E-5 0.0 0.0 0.0 12-13 2.9998530929085357E-4 8.571008836881531E-5 0.0 0.0 0.0 14-15 4.19979433007195E-4 8.571008836881531E-5 0.0 0.0 0.0 16-17 5.828286009079441E-4 8.571008836881531E-5 1.7142017673763064E-5 0.0 0.0 18-19 8.142458395037455E-4 1.0285210604257837E-4 1.7142017673763064E-5 8.571008836881532E-6 0.0 20-21 9.170979455463239E-4 1.0285210604257837E-4 2.5713026510644594E-5 1.7142017673763064E-5 0.0 22-23 0.0013370773785535187 1.0285210604257837E-4 3.428403534752613E-5 1.7142017673763064E-5 0.0 24-25 0.0018770509352770554 1.0285210604257837E-4 5.142605302128919E-5 1.7142017673763064E-5 0.0 26-27 0.002219891288752316 1.114231148794599E-4 5.142605302128919E-5 1.7142017673763064E-5 0.0 28-29 0.002725580810128327 1.2856513255322298E-4 5.142605302128919E-5 1.7142017673763064E-5 0.0 30-31 0.00347125857893702 1.371361413901045E-4 5.999706185817072E-5 1.7142017673763064E-5 0.0 32-33 0.004705483851447961 1.4570715022698604E-4 9.428109720569685E-5 1.7142017673763064E-5 0.0 34-35 0.006316833512781688 1.5427815906386756E-4 1.0285210604257837E-4 1.7142017673763064E-5 0.0 36-37 0.008416730677817664 1.5427815906386756E-4 1.0285210604257837E-4 1.7142017673763064E-5 0.0 38-39 0.011459438814910607 1.5427815906386756E-4 1.0285210604257837E-4 1.7142017673763064E-5 0.0 40-41 0.015650662136145677 1.5427815906386756E-4 1.0285210604257837E-4 1.7142017673763064E-5 0.0 42-43 0.020904690553154054 1.5427815906386756E-4 1.0285210604257837E-4 1.7142017673763064E-5 0.0 44-45 0.027384373233836492 1.5427815906386756E-4 1.0285210604257837E-4 1.7142017673763064E-5 0.0 46-47 0.03515827824888804 1.5427815906386756E-4 1.1999412371634143E-4 1.7142017673763064E-5 0.0 48-49 0.04425211862481934 1.7142017673763062E-4 1.1999412371634143E-4 1.7142017673763064E-5 0.0 50-51 0.05569441542205619 1.7142017673763062E-4 1.1999412371634143E-4 1.7142017673763064E-5 0.0 52-53 0.06862806775691041 1.7142017673763062E-4 1.1999412371634143E-4 1.7142017673763064E-5 0.0 54-55 0.08408159668980782 1.7142017673763062E-4 1.1999412371634143E-4 1.7142017673763064E-5 0.0 56-57 0.10160073875239367 1.7142017673763062E-4 1.1999412371634143E-4 1.7142017673763064E-5 0.0 58-59 0.12175975153673903 1.7999118557451214E-4 1.1999412371634143E-4 1.7142017673763064E-5 0.0 60-61 0.14522717373212066 1.971332032482752E-4 1.1999412371634143E-4 2.5713026510644594E-5 0.0 62-63 0.1701345254120984 2.0570421208515675E-4 1.1999412371634143E-4 3.428403534752613E-5 0.0 64-65 0.1980245881673109 2.228462297589198E-4 1.1999412371634143E-4 4.285504418440766E-5 0.0 66-67 0.22858023467079355 2.228462297589198E-4 1.1999412371634143E-4 5.142605302128919E-5 0.0 68-69 0.2630099771685467 2.228462297589198E-4 1.1999412371634143E-4 5.142605302128919E-5 0.0 70-71 0.30146809381963413 2.228462297589198E-4 1.1999412371634143E-4 5.142605302128919E-5 0.0 72-73 0.3426603622896867 2.3141723859580133E-4 1.1999412371634143E-4 5.142605302128919E-5 0.0 74-75 0.3864753594638251 2.3998824743268286E-4 1.1999412371634143E-4 5.142605302128919E-5 0.0 76-77 0.43629863383261747 2.3998824743268286E-4 1.1999412371634143E-4 5.142605302128919E-5 0.0 78-79 0.48997886217800646 2.3998824743268286E-4 1.1999412371634143E-4 5.999706185817072E-5 0.0 80-81 0.5490331130641202 2.3998824743268286E-4 1.1999412371634143E-4 6.856807069505225E-5 0.0 82-83 0.6129128419253982 2.4855925626956436E-4 1.1999412371634143E-4 8.571008836881531E-5 0.0 84-85 0.6801695482684076 2.571302651064459E-4 1.1999412371634143E-4 8.571008836881531E-5 0.0 86-87 0.7546516150609082 2.9141430045397207E-4 1.1999412371634143E-4 8.571008836881531E-5 0.0 88-89 0.8340620119346156 3.085563181277351E-4 1.1999412371634143E-4 8.571008836881531E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 21330 0.0 93.11122 1 TCGACGG 22480 0.0 88.034706 2 TATCTCG 22385 0.0 87.410675 2 CGACGGC 23855 0.0 82.92056 3 ATATCTC 23930 0.0 82.37915 1 ATCTCGA 23980 0.0 81.45796 3 GACGGCG 24180 0.0 81.39476 4 ACGGCGC 24210 0.0 81.11518 5 TCTCGAG 22800 0.0 80.90276 4 CGGCGCG 24525 0.0 79.9007 6 CTCGAGG 23845 0.0 76.75744 5 TCGAGGG 23905 0.0 76.37039 6 AGGGCGC 24735 0.0 73.975464 9 CGAGGGC 26870 0.0 67.991 7 GAGGGCG 27015 0.0 67.503586 8 CTCGACG 1560 0.0 64.2672 5 TCTCGAC 1885 0.0 53.440086 4 CGCGCCG 49200 0.0 42.219616 9 GCGCGCC 49330 0.0 41.86768 8 GGCGCGC 49110 0.0 41.851803 7 >>END_MODULE