FastQCFastQC Report
Thu 26 May 2016
SRR2048276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5833619
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT353380.6057646205554391No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT267610.4587375349675733No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156930.2690096833543637No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT66860.11461153016677983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG22400.084.4624561
TCGACGG30850.066.0619052
TATCTCG82350.059.1301772
ATATCTC91050.054.0681651
ATCTCGA166000.050.29761
TCTCGAG174750.046.5953522
CGACGGC46150.044.1510933
GACGGCG48950.041.62564
ACGGCGC50550.040.4020275
CTCGAGG223300.036.5843733
TCGAGGG224700.036.3141564
CGGCGCG57050.035.7155656
CGAGGGC269300.030.4587365
AGGGCGC280650.029.0915377
GAGGGCG285550.028.6588676
GGGCGCG285850.028.5459568
GGCGCGC344350.024.4410299
CTCGACG10400.023.7479363
TCTCGAC12550.020.0623192
CGCGCCG342150.019.2954169
Produced by FastQC (version 0.11.5)