##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048276_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5833619 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.232641692918236 34.0 31.0 34.0 30.0 34.0 2 32.393063893956736 34.0 31.0 34.0 30.0 34.0 3 32.46421560955558 34.0 31.0 34.0 30.0 34.0 4 35.94067747653729 37.0 35.0 37.0 35.0 37.0 5 35.902652710092994 37.0 35.0 37.0 35.0 37.0 6 35.84270895990979 37.0 35.0 37.0 35.0 37.0 7 35.768299403852055 37.0 35.0 37.0 33.0 37.0 8 35.72946244861038 37.0 35.0 37.0 33.0 37.0 9 37.35393312453213 39.0 37.0 39.0 34.0 39.0 10-11 37.34804561285199 39.0 37.0 39.0 34.0 39.0 12-13 37.36376527160927 39.0 37.0 39.0 34.0 39.0 14-15 38.752803362715326 40.0 38.0 41.0 34.0 41.0 16-17 38.80045465773476 40.0 38.0 41.0 35.0 41.0 18-19 38.888661395267675 40.0 38.0 41.0 35.0 41.0 20-21 38.971095986899385 40.0 38.0 41.0 35.0 41.0 22-23 38.98760794971355 40.0 38.0 41.0 36.0 41.0 24-25 38.99607113525926 40.0 38.0 41.0 36.0 41.0 26-27 38.88379931908477 40.0 38.0 41.0 35.0 41.0 28-29 38.73091969153282 40.0 38.0 41.0 35.0 41.0 30-31 38.59835438344534 40.0 38.0 41.0 35.0 41.0 32-33 38.48181840466441 40.0 38.0 41.0 35.0 41.0 34-35 38.417273308387124 40.0 38.0 41.0 35.0 41.0 36-37 38.339562285435505 40.0 38.0 41.0 34.0 41.0 38-39 38.22456943108557 40.0 38.0 41.0 34.0 41.0 40-41 38.06588577347955 40.0 37.5 41.0 34.0 41.0 42-43 37.86154589458105 40.0 37.0 41.0 33.0 41.0 44-45 37.61515287508492 40.0 37.0 41.0 33.0 41.0 46-47 37.303643930122966 40.0 37.0 41.0 33.0 41.0 48-49 37.00976280761565 40.0 37.0 41.0 32.0 41.0 50-51 36.75868710315157 40.0 36.0 41.0 31.0 41.0 52-53 36.556665082172835 40.0 36.0 41.0 31.0 41.0 54-55 36.32327179748969 39.0 35.5 41.0 31.0 41.0 56-57 36.07703262760218 39.0 35.0 41.0 30.0 41.0 58-59 35.83178375207568 39.0 35.0 41.0 30.0 41.0 60-61 35.56981386682949 38.5 35.0 40.0 29.0 41.0 62-63 35.27206541942489 38.0 35.0 40.0 29.0 41.0 64-65 34.95413001774713 37.0 35.0 40.0 29.0 41.0 66-67 34.63247762666708 37.0 34.0 40.0 28.0 41.0 68-69 34.285426593680526 36.0 34.0 39.0 28.0 41.0 70-71 33.95755447861782 36.0 34.0 39.0 28.0 41.0 72-73 33.6031665763568 35.0 34.0 38.5 27.0 40.0 74-75 33.27571966904249 35.0 34.0 37.5 26.5 40.0 76-77 32.973443757639984 35.0 33.5 37.0 26.0 39.0 78-79 32.67560162910879 35.0 33.0 36.5 26.0 39.0 80-81 32.41408283605769 35.0 33.0 36.0 26.0 38.5 82-83 32.16749388329954 35.0 33.0 36.0 26.0 37.0 84-85 31.9215888113365 35.0 33.0 35.0 25.0 37.0 86-87 31.744761270833763 35.0 33.0 35.0 25.0 36.5 88-89 31.595903846308783 35.0 33.0 35.0 25.0 36.0 90-91 31.44229139750128 35.0 33.0 35.0 25.0 36.0 92-93 31.251295122290298 35.0 33.0 35.0 24.0 36.0 94-95 31.1244946747465 35.0 33.0 35.0 24.0 35.5 96-97 31.007574114799063 35.0 33.0 35.0 23.0 35.0 98-99 30.866350030744208 35.0 33.0 35.0 21.5 35.0 100-101 29.998049752649255 34.5 31.5 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4329316956010203 1101 2 0.41927561887674614 1101 3 0.381455649944094 1101 4 0.3074382328898224 1101 5 0.2959769813313571 1101 6 0.251240791313343 1101 7 0.22956604560715732 1101 8 0.2374664215523552 1101 9 0.2665321016404647 1101 10-11 0.32853653154826645 1101 12-13 0.3433895541098906 1101 14-15 0.44993917800157845 1101 16-17 0.4735569507328137 1101 18-19 0.5294703030734667 1101 20-21 0.5148002636506561 1101 22-23 0.4914546798034678 1101 24-25 0.5102318698967565 1101 26-27 0.5699911004449945 1101 28-29 0.5095202805763535 1101 30-31 0.471958755501511 1101 32-33 0.4944030200647731 1101 34-35 0.4924807815053569 1101 36-37 0.5467557262079197 1101 38-39 0.5203356382540676 1101 40-41 0.458615278194344 1101 42-43 0.48392902670160964 1101 44-45 0.42006037564927823 1101 46-47 0.4012763234079131 1101 48-49 0.3774984769424776 1101 50-51 0.4251530325426458 1101 52-53 0.38967578130903036 1101 54-55 0.38505539890334717 1101 56-57 0.3779003745761642 1101 58-59 0.3805282850534226 1101 60-61 0.2924793129569494 1101 62-63 0.350825232737094 1101 64-65 0.3208929784113792 1101 66-67 0.3598704843527756 1101 68-69 0.3243850426681405 1101 70-71 0.3584324499862035 1101 72-73 0.3428041610782131 1101 74-75 0.3589999885203099 1101 76-77 0.34248747824562287 1101 78-79 0.35344352033902027 1101 80-81 0.34846297238250656 1101 82-83 0.3965493894760712 1101 84-85 0.3737987950726698 1101 86-87 0.3658299857014278 1101 88-89 0.4065474810066121 1101 90-91 0.4194649550705378 1101 92-93 0.47408127699852187 1101 94-95 0.4630606862357851 1101 96-97 0.41288648052009336 1101 98-99 0.46907255142491877 1101 100-101 0.6341027345654275 1102 1 0.44985480615572015 1102 2 0.4057535233110343 1102 3 0.35411168352325006 1102 4 0.28025613261419835 1102 5 0.24501780269164186 1102 6 0.24506402722722953 1102 7 0.22964691105238444 1102 8 0.1910107195265951 1102 9 0.2714186197612136 1102 10-11 0.25425500953242874 1102 12-13 0.24329864092599252 1102 14-15 0.362392595202337 1102 16-17 0.36206209084517127 1102 18-19 0.3867851439013492 1102 20-21 0.39012376981202834 1102 22-23 0.43421128561681854 1102 24-25 0.443758554994929 1102 26-27 0.4190324577523086 1102 28-29 0.4578237521080979 1102 30-31 0.4619009403431491 1102 32-33 0.4373484077224461 1102 34-35 0.4688515535110085 1102 36-37 0.46669872409979263 1102 38-39 0.5056340281119773 1102 40-41 0.48598674360047767 1102 42-43 0.5254984134887835 1102 44-45 0.4652051969039306 1102 46-47 0.4237265609381069 1102 48-49 0.37216517005144567 1102 50-51 0.41037185032906365 1102 52-53 0.3910101287826251 1102 54-55 0.35853857457104255 1102 56-57 0.35616530509232547 1102 58-59 0.3660283313190007 1102 60-61 0.38914991198696924 1102 62-63 0.3759002327747112 1102 64-65 0.39578287637975507 1102 66-67 0.2921109388615477 1102 68-69 0.3255557318708213 1102 70-71 0.286953313803636 1102 72-73 0.3190451054121297 1102 74-75 0.3383681245626704 1102 76-77 0.33344103062506036 1102 78-79 0.36234270932943957 1102 80-81 0.4085019812105344 1102 82-83 0.3714881938783492 1102 84-85 0.3843947974116979 1102 86-87 0.3375979956390047 1102 88-89 0.4012469311300322 1102 90-91 0.4318933721178624 1102 92-93 0.4356609424718876 1102 94-95 0.4954355636970753 1102 96-97 0.44115072884500606 1102 98-99 0.4827377296297577 1102 100-101 0.5642269760461183 1103 1 0.39285872977725944 1103 2 0.3439984093386883 1103 3 0.34935340228239653 1103 4 0.26937982441514663 1103 5 0.2589925030048619 1103 6 0.21289440202670562 1103 7 0.20468930504166138 1103 8 0.17203665446945138 1103 9 0.278960087016884 1103 10-11 0.23975522320215958 1103 12-13 0.24946699754547552 1103 14-15 0.3360841265991539 1103 16-17 0.3540119600886271 1103 18-19 0.33457777990347637 1103 20-21 0.3687433676108469 1103 22-23 0.36155016451978383 1103 24-25 0.3748899730224764 1103 26-27 0.3981785818574437 1103 28-29 0.3803380723249603 1103 30-31 0.36896275908733145 1103 32-33 0.4010961739665504 1103 34-35 0.44838382801983556 1103 36-37 0.42639850505512555 1103 38-39 0.39225533398121826 1103 40-41 0.36080918369349035 1103 42-43 0.3570247926281169 1103 44-45 0.33458959554574363 1103 46-47 0.23717562808528214 1103 48-49 0.2788691292135326 1103 50-51 0.3725610948442366 1103 52-53 0.35818869624839067 1103 54-55 0.34813995158413036 1103 56-57 0.3901157473938852 1103 58-59 0.37654643394468224 1103 60-61 0.4183451708765489 1103 62-63 0.4334071853889583 1103 64-65 0.4033460928079933 1103 66-67 0.356750179792094 1103 68-69 0.36333064105012625 1103 70-71 0.4236824764186551 1103 72-73 0.3791224272207927 1103 74-75 0.3386187348953058 1103 76-77 0.29396663643365883 1103 78-79 0.3105202002795622 1103 80-81 0.29196328437943464 1103 82-83 0.32238141059062997 1103 84-85 0.33794989398792197 1103 86-87 0.39354848637934836 1103 88-89 0.38702487923922746 1103 90-91 0.37396257214338746 1103 92-93 0.3788918926950906 1103 94-95 0.395767441748184 1103 96-97 0.43688002977607 1103 98-99 0.402484908171477 1103 100-101 0.539539870843285 1104 1 0.35636982367517334 1104 2 0.3228720306923307 1104 3 0.2987082892817341 1104 4 0.23831978700663825 1104 5 0.24392692091150536 1104 6 0.15740173855892436 1104 7 0.17639170122132697 1104 8 0.14875068287371107 1104 9 0.15173673924826403 1104 10-11 0.15696526862829785 1104 12-13 0.1645606013935108 1104 14-15 0.2628002592986718 1104 16-17 0.31017069959978016 1104 18-19 0.29723820578087157 1104 20-21 0.29710026461172134 1104 22-23 0.29643549108244116 1104 24-25 0.3152364518972206 1104 26-27 0.31615789370494696 1104 28-29 0.3414452086561255 1104 30-31 0.3160444744662172 1104 32-33 0.28035773147534826 1104 34-35 0.3845851648599776 1104 36-37 0.3641049723802112 1104 38-39 0.3343396411060482 1104 40-41 0.36253747602326314 1104 42-43 0.3722863671843655 1104 44-45 0.3236250451963443 1104 46-47 0.3740163510465493 1104 48-49 0.30770671118116155 1104 50-51 0.2918116141256988 1104 52-53 0.3310411031093494 1104 54-55 0.295331143590154 1104 56-57 0.3169213259605499 1104 58-59 0.3354513054148569 1104 60-61 0.28838133811847655 1104 62-63 0.30326739092799926 1104 64-65 0.343380496703773 1104 66-67 0.2650944829853472 1104 68-69 0.2816996470716049 1104 70-71 0.29513336935903567 1104 72-73 0.25895970925974865 1104 74-75 0.2150954307575148 1104 76-77 0.25359197598434235 1104 78-79 0.32462238805408106 1104 80-81 0.22699841545572497 1104 82-83 0.23670915750837196 1104 84-85 0.19129463135854863 1104 86-87 0.21543534135291864 1104 88-89 0.2615041993440883 1104 90-91 0.254494165073595 1104 92-93 0.2736836287280262 1104 94-95 0.32983273243659994 1104 96-97 0.3467208331800222 1104 98-99 0.31020974945700175 1104 100-101 0.4330809629352359 1105 1 0.2751111707496747 1105 2 0.24782318036460538 1105 3 0.2021335972057372 1105 4 0.14102418146821094 1105 5 0.1702067538649885 1105 6 0.13022566065163232 1105 7 0.12756869548382355 1105 8 0.13702549227829763 1105 9 0.18164271984075242 1105 10-11 0.14946532523622125 1105 12-13 0.22045248551548013 1105 14-15 0.23116755651984988 1105 16-17 0.2514265053777649 1105 18-19 0.24041452923947304 1105 20-21 0.2448802924581983 1105 22-23 0.2803107167632106 1105 24-25 0.26635703318665804 1105 26-27 0.25590249174940993 1105 28-29 0.28181291264566255 1105 30-31 0.201678922790812 1105 32-33 0.26094103309668526 1105 34-35 0.2477276099202541 1105 36-37 0.3162440125316337 1105 38-39 0.2986908732872493 1105 40-41 0.2969968434909447 1105 42-43 0.3260527840599039 1105 44-45 0.24147438424684964 1105 46-47 0.16091491929352486 1105 48-49 0.14725937081782092 1105 50-51 0.20475903189171873 1105 52-53 0.21964528687392004 1105 54-55 0.15572113666088683 1105 56-57 0.16443190442191025 1105 58-59 0.1647829495215376 1105 60-61 0.18454527183545366 1105 62-63 0.1387020190578312 1105 64-65 0.05492566543696853 1105 66-67 0.03119347326172317 1105 68-69 0.0985108458980335 1105 70-71 0.08809783044235786 1105 72-73 0.1280033282066384 1105 74-75 0.10852240746000064 1105 76-77 0.12042089655673038 1105 78-79 0.15736470289303384 1105 80-81 0.08152406589232442 1105 82-83 0.16405138544837428 1105 84-85 0.13697237521394 1105 86-87 0.1727642811604504 1105 88-89 0.14870449215724335 1105 90-91 0.2107424350661269 1105 92-93 0.2182544855016637 1105 94-95 0.2548500065178416 1105 96-97 0.32748088293492117 1105 98-99 0.3643148665591589 1105 100-101 0.49130639319011493 1106 1 0.2572113347065752 1106 2 0.23422111719639105 1106 3 0.2239676903553942 1106 4 0.172989763040718 1106 5 0.16782590268618947 1106 6 0.12804110634024823 1106 7 0.1423468923797131 1106 8 0.09662186374347215 1106 9 0.12450418614787395 1106 10-11 0.08753189252708182 1106 12-13 0.1158005133538822 1106 14-15 0.09460107456828837 1106 16-17 0.15963082479848367 1106 18-19 0.19708131617194624 1106 20-21 0.16998978377006324 1106 22-23 0.20074685166389372 1106 24-25 0.20659139171189622 1106 26-27 0.25207717404997254 1106 28-29 0.2267888743108557 1106 30-31 0.19215840116009275 1106 32-33 0.06662776899692346 1106 34-35 0.08329121504772985 1106 36-37 0.14390343557206364 1106 38-39 0.18246195769605578 1106 40-41 0.17232588580605324 1106 42-43 0.14322000361980258 1106 44-45 0.036794989832984015 1106 46-47 -0.058627640635094735 1106 48-49 -0.05003796811166694 1106 50-51 0.024953696109577095 1106 52-53 0.09250208423163286 1106 54-55 0.08147870418107317 1106 56-57 0.08723694629417622 1106 58-59 0.06933522462117736 1106 60-61 0.03667948749149019 1106 62-63 0.12669924404782051 1106 64-65 0.12727774178500084 1106 66-67 0.03056718493481725 1106 68-69 0.07745419707683965 1106 70-71 0.0765630212333619 1106 72-73 0.051442854603166666 1106 74-75 0.07764440773414805 1106 76-77 0.020815846183474207 1106 78-79 0.08487366187218015 1106 80-81 0.1261888933158275 1106 82-83 0.1907378745565751 1106 84-85 0.18235422575219573 1106 86-87 0.2061550537743777 1106 88-89 0.18631894237444513 1106 90-91 0.15768667851839524 1106 92-93 0.20271082967144238 1106 94-95 0.20411686284204222 1106 96-97 0.19658991756620026 1106 98-99 0.1602896028310603 1106 100-101 0.3064333362243197 1107 1 0.21771522662154297 1107 2 0.1769374801935939 1107 3 0.17564622585805267 1107 4 0.15289152685080865 1107 5 0.1108814322993723 1107 6 0.12348738143830218 1107 7 0.06234412093586883 1107 8 0.08058181318696711 1107 9 0.07788917114839222 1107 10-11 0.10846196098462713 1107 12-13 0.06503120620047298 1107 14-15 0.11805465759339029 1107 16-17 0.1607211628523686 1107 18-19 0.13655943222232736 1107 20-21 0.16604115478067172 1107 22-23 0.16726682018820327 1107 24-25 0.12481883146451622 1107 26-27 0.11769978385925839 1107 28-29 0.1546749395518603 1107 30-31 0.09334433486926486 1107 32-33 0.18544679866270997 1107 34-35 0.18179712583820873 1107 36-37 0.13240154449267294 1107 38-39 0.10557414705208856 1107 40-41 0.1376610860324945 1107 42-43 0.1431047396533458 1107 44-45 0.12717634753075657 1107 46-47 0.07512801061190544 1107 48-49 0.0243056937990076 1107 50-51 0.014547451293061897 1107 52-53 0.0239867160486682 1107 54-55 -0.047931319920593296 1107 56-57 -0.0017161040740987232 1107 58-59 -0.06414990752448801 1107 60-61 -0.025394708338794203 1107 62-63 -0.051659012324464015 1107 64-65 -0.025427776501345534 1107 66-67 -0.0018079807571282913 1107 68-69 -0.028368022144050542 1107 70-71 -0.019633659004760773 1107 72-73 -0.057428312051769126 1107 74-75 -0.05317576403470525 1107 76-77 -0.10378729535029407 1107 78-79 -0.06242945665670163 1107 80-81 -0.08092221492167084 1107 82-83 -0.00574360506237781 1107 84-85 -0.060752885284198754 1107 86-87 -0.05212403604898341 1107 88-89 -0.059273727539725485 1107 90-91 -0.06747545334134841 1107 92-93 -0.025859092531767658 1107 94-95 -0.012608728935454394 1107 96-97 0.044155517051379434 1107 98-99 -0.027693194740347593 1107 100-101 0.06160329230428019 1108 1 0.17964816337462253 1108 2 0.11411835171212203 1108 3 0.08450243105954058 1108 4 0.0720272656043619 1108 5 0.10876337772029387 1108 6 0.010719105427185127 1108 7 0.06090003027311752 1108 8 0.09159194216671551 1108 9 0.07991943272056545 1108 10-11 0.03188895959281268 1108 12-13 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-0.28949810064829506 2214 78-79 -0.3696395809733133 2214 80-81 -0.36420574337749656 2214 82-83 -0.3172352684497426 2214 84-85 -0.3898239731762665 2214 86-87 -0.39283324581496615 2214 88-89 -0.38317540972166597 2214 90-91 -0.4496133234932529 2214 92-93 -0.3581265835023153 2214 94-95 -0.30820083429474465 2214 96-97 -0.38564690786504485 2214 98-99 -0.4241224176932157 2214 100-101 -0.5044391273787561 2215 1 -0.5201106448640864 2215 2 -0.5146840422856229 2215 3 -0.46549007061655345 2215 4 -0.3857136684290694 2215 5 -0.366101107588527 2215 6 -0.3634344574788244 2215 7 -0.4055190677416505 2215 8 -0.40164979341764706 2215 9 -0.4405143647766039 2215 10-11 -0.39704452966876147 2215 12-13 -0.459183854045385 2215 14-15 -0.5774432469598594 2215 16-17 -0.6098604906417506 2215 18-19 -0.6679470928072675 2215 20-21 -0.5909047293702514 2215 22-23 -0.5483930984011351 2215 24-25 -0.5999246260968576 2215 26-27 -0.5391457796296351 2215 28-29 -0.4798727335407733 2215 30-31 -0.6163564360946552 2215 32-33 -0.6505985550517437 2215 34-35 -0.6720106893863687 2215 36-37 -0.6237277388807954 2215 38-39 -0.6715979524703215 2215 40-41 -0.6047400032604742 2215 42-43 -0.6040219302441514 2215 44-45 -0.5902820037860224 2215 46-47 -0.5740108077865997 2215 48-49 -0.5886695021277433 2215 50-51 -0.6088313876640044 2215 52-53 -0.5914703718317824 2215 54-55 -0.5645165286575562 2215 56-57 -0.570084539145121 2215 58-59 -0.4788474754913068 2215 60-61 -0.5184964670577159 2215 62-63 -0.49143609406492317 2215 64-65 -0.48684131581623546 2215 66-67 -0.47241183297864353 2215 68-69 -0.4151018434959042 2215 70-71 -0.4574409792648808 2215 72-73 -0.42275785887606077 2215 74-75 -0.4042777337829335 2215 76-77 -0.4065135699301763 2215 78-79 -0.39160285938226025 2215 80-81 -0.3726622624333089 2215 82-83 -0.42650459811608243 2215 84-85 -0.42386139950357915 2215 86-87 -0.46632891510303764 2215 88-89 -0.4214013597944799 2215 90-91 -0.44559383547829867 2215 92-93 -0.485720179046357 2215 94-95 -0.5442620087620753 2215 96-97 -0.5360050088216255 2215 98-99 -0.5565790567844502 2215 100-101 -0.5809973513527744 2216 1 -0.6176143738150941 2216 2 -0.6397115852669906 2216 3 -0.6240221202667939 2216 4 -0.45929197811826583 2216 5 -0.46351358139853716 2216 6 -0.4528266262951419 2216 7 -0.3810971947415993 2216 8 -0.3821262043126836 2216 9 -0.4668066370786548 2216 10-11 -0.45615747672410123 2216 12-13 -0.4692233691858263 2216 14-15 -0.6895330695078954 2216 16-17 -0.6602331316095587 2216 18-19 -0.7765989732179648 2216 20-21 -0.7504494891668259 2216 22-23 -0.773674024283423 2216 24-25 -0.6726107060494542 2216 26-27 -0.7020674896983579 2216 28-29 -0.753579034873809 2216 30-31 -0.6978680460966373 2216 32-33 -0.7485227574113011 2216 34-35 -0.8274774861881085 2216 36-37 -0.8336538463770964 2216 38-39 -0.7801427481318299 2216 40-41 -0.8084538351560155 2216 42-43 -0.7315771457788145 2216 44-45 -0.6789296978067298 2216 46-47 -0.6773507495264823 2216 48-49 -0.7020213981300216 2216 50-51 -0.6160636126157542 2216 52-53 -0.6195367414327464 2216 54-55 -0.5814157226343042 2216 56-57 -0.6099212291309115 2216 58-59 -0.6414082454377237 2216 60-61 -0.6193986849281075 2216 62-63 -0.6260209531493643 2216 64-65 -0.630378010727199 2216 66-67 -0.5869933736617909 2216 68-69 -0.5579782943076808 2216 70-71 -0.6286239104050679 2216 72-73 -0.6668242523869807 2216 74-75 -0.6223059877633688 2216 76-77 -0.6318828874856095 2216 78-79 -0.6960371594748409 2216 80-81 -0.6884313494509442 2216 82-83 -0.6588918547964795 2216 84-85 -0.6248538899875591 2216 86-87 -0.5512601231514402 2216 88-89 -0.6322465330901217 2216 90-91 -0.7227644228528689 2216 92-93 -0.6255596716513097 2216 94-95 -0.6814133761825119 2216 96-97 -0.7121567546493424 2216 98-99 -0.8078649596602006 2216 100-101 -1.0099679396722863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 164.0 3 49.0 4 71.0 5 118.0 6 271.0 7 682.0 8 1599.0 9 3017.0 10 4671.0 11 5390.0 12 6243.0 13 9182.0 14 15761.0 15 27618.0 16 38159.0 17 51781.0 18 50599.0 19 32701.0 20 26477.0 21 23128.0 22 21812.0 23 21467.0 24 22727.0 25 25858.0 26 31511.0 27 39452.0 28 48743.0 29 62055.0 30 81166.0 31 108424.0 32 148136.0 33 205141.0 34 290234.0 35 425180.0 36 741059.0 37 1364555.0 38 1517723.0 39 380353.0 40 342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.161615230764813 37.162246384448764 17.60648848681807 21.06964989796836 2 23.313599748445228 29.05622211435969 29.416854821246435 18.213323315948646 3 22.313913017243507 28.061085954234905 24.55922707288241 25.065773955639177 4 23.618306234946093 26.624193318075797 26.793745014886984 22.963755432091126 5 23.31950372487473 27.167938118687555 25.369689038656794 24.14286911778092 6 24.77484388335954 25.904588558148895 26.125703444122767 23.194864114368798 7 23.874185132762353 27.710122995691012 24.66842623764082 23.747265633905815 8 25.43966576950445 25.990391147527102 25.844083023474955 22.725860059493495 9 23.446131809430817 26.20220826900077 26.14263975758444 24.20902016398397 10-11 24.379291825537457 26.146291007348953 25.60393471016876 23.87048245694482 12-13 23.968869067383388 26.27441044744266 25.632656160781153 24.124064324392798 14-15 23.30144202975255 26.359115934495193 26.17873043613161 24.160711599620647 16-17 23.353564913992496 26.3902904869173 26.24180632982716 24.014338269263042 18-19 22.697822740909203 26.544037243433277 27.4219999626304 23.33614005302712 20-21 21.7642054865613 26.349842100549104 28.47620708762616 23.40974532526344 22-23 21.430215901828646 26.08633193174743 29.374478862384013 23.108973304039914 24-25 21.432520232385084 25.66544323541255 30.59358957678398 22.308446955418386 26-27 21.384157794766924 25.568189172409284 31.175604683673892 21.872048349149903 28-29 21.463013839540196 25.770149624759448 31.123758663106415 21.64307787259394 30-31 21.463668516760258 25.617580486271212 31.036237839983947 21.882513156984583 32-33 21.457902191404518 25.607149974179695 31.090268209742288 21.844679624673496 34-35 21.544353652591226 25.763729229849364 30.960072421683172 21.731844695876244 36-37 21.619812675459265 25.653080874839446 30.797031825355752 21.93007462434554 38-39 21.637126113309765 25.620622464374172 30.832652938081832 21.90959848423423 40-41 21.69763743569815 25.924987559180675 30.470013554193375 21.907361450927805 42-43 21.89403353218645 25.889589292684352 30.03834326513267 22.178033909996522 44-45 21.881168447922295 26.02599689832332 29.781187286999717 22.31164736675467 46-47 22.195073075564242 26.362220432976514 29.00545956120892 22.43724693025033 48-49 22.400574532159816 26.436698953102155 28.36824535588218 22.794481158855845 50-51 22.482887552306725 26.39218467987025 28.121797121135266 23.003130646687758 52-53 22.60947413758006 26.571332451931834 27.814374272790193 23.00481913769791 54-55 22.650676473555404 26.474356815061682 27.62826220947619 23.246704501906716 56-57 22.68011607905417 26.43417941167092 27.70409407011441 23.181610439160497 58-59 22.811458662981128 26.691202462574008 27.410313228689482 23.087025645755382 60-61 22.758862514574187 26.616509955873102 27.30986532737158 23.31476220218114 62-63 22.79412338229772 26.592078241989498 27.43576440421131 23.178033971501474 64-65 22.876844674581825 26.858088215253073 27.176569870802282 23.08849723936282 66-67 22.915135840509805 26.707836301418297 27.088588282939103 23.288439575132795 68-69 22.888573322524692 26.721641839528676 27.17751790788643 23.212266930060206 70-71 22.947132582310843 26.924194783583555 27.00327970580786 23.125392928297735 72-73 22.9319400357964 26.841671113646637 26.8627731978503 23.363615652706663 74-75 22.871424003038964 26.81402994446535 27.048097771422984 23.266448281072698 76-77 22.950815597381208 26.923768868577465 26.902324162192947 23.22309137184838 78-79 22.971282147454318 26.88461175178266 26.803220475220147 23.340885625542874 80-81 22.891400005519817 26.846777258423593 26.984609825239513 23.277212910817074 82-83 22.925050721091893 27.007808275429735 26.816769043090495 23.25037196038788 84-85 22.965148845179055 26.86239150504762 26.74873553447063 23.423724115302697 86-87 22.899748765470722 26.804855658005604 26.941187462445537 23.354208114078133 88-89 22.98426138498715 26.932260937898572 26.735845395124812 23.347632281989462 90-91 22.96271522373343 26.86877083174945 26.665213563707578 23.503300380809545 92-93 22.915681513455898 26.8606056741225 26.853234435307822 23.370478377113777 94-95 22.99202015959681 27.03479930401392 26.64660706785864 23.32657346853063 96-97 22.98081081789948 26.961701691436772 26.554420776889394 23.503066713774352 98-99 22.91210019899678 26.961316524361013 26.71541832050901 23.411164956133195 100-101 23.047490920535786 27.064611706123294 26.46565677448198 23.422240598858934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 721.0 1 501.5 2 232.0 3 174.5 4 146.5 5 120.0 6 128.5 7 133.5 8 137.5 9 145.0 10 148.5 11 156.5 12 211.0 13 393.0 14 576.5 15 816.5 16 3297.0 17 3852.0 18 2201.0 19 2595.0 20 3448.0 21 4995.0 22 7378.5 23 10836.5 24 15395.5 25 20794.5 26 27312.0 27 35314.0 28 44897.0 29 55184.5 30 65959.0 31 78225.5 32 92606.5 33 107348.0 34 118324.5 35 130291.5 36 144804.0 37 162009.0 38 175701.5 39 183672.0 40 191597.0 41 201285.0 42 215495.0 43 227428.0 44 233103.0 45 233660.5 46 232704.0 47 230416.5 48 230857.0 49 227878.0 50 219788.0 51 211645.5 52 192991.5 53 170298.5 54 155212.5 55 143644.5 56 130602.0 57 117305.5 58 107628.0 59 99377.0 60 88549.5 61 77950.0 62 69508.5 63 61741.5 64 53710.0 65 45397.0 66 37797.0 67 31335.0 68 25316.0 69 19846.5 70 14958.0 71 11052.5 72 8023.0 73 5571.5 74 3682.0 75 2210.0 76 1324.0 77 855.0 78 532.5 79 281.0 80 121.0 81 46.5 82 28.0 83 15.0 84 8.0 85 6.0 86 6.0 87 4.0 88 1.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2963169175086683 2 0.01871908329974926 3 5.142605302128919E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 6.856807069505225E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.5713026510644594E-5 16-17 0.0 18-19 0.0 20-21 2.5713026510644594E-5 22-23 0.003891238011944215 24-25 4.456924595178396E-4 26-27 0.00940239669405904 28-29 0.011896560265591565 30-31 0.005322596487703431 32-33 5.399735567235364E-4 34-35 1.1999412371634144E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 5.142605302128919E-5 50-51 0.0 52-53 0.001825624882255766 54-55 0.002888429978029076 56-57 0.006051132238838361 58-59 0.006393972592313621 60-61 0.001757056811560714 62-63 0.003934093056128623 64-65 3.428403534752612E-5 66-67 5.399735567235364E-4 68-69 2.7427228278020896E-4 70-71 2.828432916170905E-4 72-73 0.0012342252725109405 74-75 9.170979455463238E-4 76-77 1.9713320324827522E-4 78-79 0.0011999412371634143 80-81 0.0015770656259862018 82-83 8.57100883688153E-6 84-85 0.00386552498543357 86-87 0.007268215493675538 88-89 0.005536871708625469 90-91 0.002151323218057264 92-93 0.0023227433947948946 94-95 0.0028970009868659577 96-97 0.003016995110582299 98-99 0.0018427668999295291 100-101 0.0021427522092203827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5833619.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.13199815541992 #Duplication Level Percentage of deduplicated Percentage of total 1 79.97541993399577 42.49253864411997 2 11.840411735459224 12.582094689756637 3 3.1690573348737483 5.0513504541279595 4 1.3105785571935291 2.78534629933358 5 0.6946883319307046 1.845508958536697 6 0.46541010573523234 1.4836901327662895 7 0.3288600199233997 1.2231092980373002 8 0.25602834262712826 1.088263794255984 9 0.19879735237751675 0.9506250503842126 >10 1.5029891128725883 15.21090919691509 >50 0.1767891384537508 6.641797855083413 >100 0.07970852240092292 6.250890620871677 >500 4.852000308335835E-4 0.17146868229317824 >1k 5.498877556961711E-4 0.4877975043818962 >5k 1.2938535426572576E-4 0.3976555915700752 >10k+ 9.703901569929431E-5 1.3369532275658782 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35338 0.6057646205554391 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 26761 0.4587375349675733 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15693 0.2690096833543637 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 6686 0.11461153016677983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7142017673763064E-5 0.0 0.0 0.0 0.0 2 1.7142017673763064E-5 0.0 0.0 0.0 0.0 3 1.7142017673763064E-5 0.0 0.0 0.0 0.0 4 1.7142017673763064E-5 0.0 0.0 0.0 0.0 5 6.856807069505225E-5 0.0 0.0 1.7142017673763064E-5 0.0 6 6.856807069505225E-5 0.0 0.0 1.7142017673763064E-5 0.0 7 8.571008836881531E-5 0.0 0.0 1.7142017673763064E-5 0.0 8 1.0285210604257837E-4 0.0 0.0 1.7142017673763064E-5 0.0 9 1.0285210604257837E-4 0.0 0.0 3.428403534752613E-5 0.0 10-11 1.885621944113937E-4 0.0 0.0 3.428403534752613E-5 0.0 12-13 2.9998530929085357E-4 0.0 0.0 3.428403534752613E-5 0.0 14-15 4.19979433007195E-4 0.0 0.0 3.428403534752613E-5 0.0 16-17 5.828286009079441E-4 1.7142017673763064E-5 0.0 3.428403534752613E-5 0.0 18-19 8.05674830666864E-4 2.5713026510644594E-5 0.0 5.142605302128919E-5 0.0 20-21 9.428109720569684E-4 4.285504418440766E-5 0.0 5.142605302128919E-5 0.0 22-23 0.0014142164580854526 5.999706185817072E-5 0.0 5.142605302128919E-5 0.0 24-25 0.001988474050156515 8.571008836881531E-5 0.0 5.142605302128919E-5 0.0 26-27 0.0023484564213055396 9.428109720569685E-5 0.0 5.142605302128919E-5 0.0 28-29 0.0028712879603553128 1.0285210604257837E-4 0.0 5.142605302128919E-5 0.0 30-31 0.0036255367380008878 1.2856513255322298E-4 0.0 5.142605302128919E-5 0.0 32-33 0.004902617054696235 1.7999118557451214E-4 0.0 5.142605302128919E-5 0.0 34-35 0.0065396797425406085 1.8856219441139367E-4 0.0 5.142605302128919E-5 0.0 36-37 0.00862243488990282 1.8856219441139367E-4 0.0 5.142605302128919E-5 0.0 38-39 0.011613716973974475 1.8856219441139367E-4 0.0 5.999706185817072E-5 0.0 40-41 0.015804940295209544 2.0570421208515675E-4 0.0 6.856807069505225E-5 0.0 42-43 0.021076110729891684 2.3141723859580133E-4 0.0 6.856807069505225E-5 0.0 44-45 0.02759864845475853 2.3998824743268286E-4 0.0 8.571008836881531E-5 0.0 46-47 0.03538112447864696 2.6570127394332746E-4 0.0 8.571008836881531E-5 0.0 48-49 0.04450924888992579 2.74272282780209E-4 0.0 8.571008836881531E-5 0.0 50-51 0.05595154568716264 2.8284329161709057E-4 0.0 8.571008836881531E-5 0.0 52-53 0.06885091398666934 2.9141430045397207E-4 0.0 1.0285210604257837E-4 0.0 54-55 0.08434729796375115 2.9141430045397207E-4 0.0 1.0285210604257837E-4 0.0 56-57 0.1019607211235427 2.9141430045397207E-4 0.0 1.0285210604257837E-4 0.0 58-59 0.12199116877533484 3.256983358014982E-4 0.0 1.0285210604257837E-4 0.0 60-61 0.1453985939088583 3.256983358014982E-4 0.0 1.0285210604257837E-4 0.0 62-63 0.17019452247395656 3.256983358014982E-4 0.0 1.0285210604257837E-4 0.0 64-65 0.1978360259728995 3.256983358014982E-4 0.0 1.0285210604257837E-4 0.0 66-67 0.22845166953824034 3.256983358014982E-4 0.0 1.0285210604257837E-4 0.0 68-69 0.26269284984158203 3.256983358014982E-4 0.0 1.114231148794599E-4 0.0 70-71 0.30097097530709493 3.342693446383797E-4 0.0 1.1999412371634143E-4 0.0 72-73 0.34182040342367237 3.4284035347526123E-4 0.0 1.1999412371634143E-4 0.0 74-75 0.3854639804210731 3.4284035347526123E-4 0.0 1.1999412371634143E-4 0.0 76-77 0.4349444144363902 3.4284035347526123E-4 0.0 1.1999412371634143E-4 0.0 78-79 0.48853036168457353 3.4284035347526123E-4 0.0 1.2856513255322298E-4 0.0 80-81 0.5474303344116234 3.4284035347526123E-4 0.0 1.371361413901045E-4 0.0 82-83 0.6109757939282631 3.514113623121428E-4 0.0 1.371361413901045E-4 0.0 84-85 0.6782325002712725 3.599823711490243E-4 0.0 1.5427815906386756E-4 0.0 86-87 0.7525774309223828 3.599823711490243E-4 0.0 1.5427815906386756E-4 0.0 88-89 0.8316535584514518 3.685533799859058E-4 0.0 1.5427815906386756E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2240 0.0 84.462456 1 TCGACGG 3085 0.0 66.061905 2 TATCTCG 8235 0.0 59.130177 2 ATATCTC 9105 0.0 54.068165 1 ATCTCGA 16600 0.0 50.2976 1 TCTCGAG 17475 0.0 46.595352 2 CGACGGC 4615 0.0 44.151093 3 GACGGCG 4895 0.0 41.6256 4 ACGGCGC 5055 0.0 40.402027 5 CTCGAGG 22330 0.0 36.584373 3 TCGAGGG 22470 0.0 36.314156 4 CGGCGCG 5705 0.0 35.715565 6 CGAGGGC 26930 0.0 30.458736 5 AGGGCGC 28065 0.0 29.091537 7 GAGGGCG 28555 0.0 28.658867 6 GGGCGCG 28585 0.0 28.545956 8 GGCGCGC 34435 0.0 24.441029 9 CTCGACG 1040 0.0 23.747936 3 TCTCGAC 1255 0.0 20.062319 2 CGCGCCG 34215 0.0 19.295416 9 >>END_MODULE