FastQCFastQC Report
Thu 26 May 2016
SRR2048275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6584975
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT599970.9111196321929848No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG123270.18719888837846765No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC120510.1830075285023861No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA114680.17415404006848925No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC89080.13527765860918226No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA85800.13029662223470856No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG84750.12870208315141668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG231400.092.826391
TATCTCG271400.088.597312
TCGACGG246400.086.612832
ATCTCGA289100.083.0250553
ATATCTC293350.082.52191
CGACGGC259750.082.2161943
TCTCGAG276800.081.61684
GACGGCG263600.080.493824
ACGGCGC263000.080.3708955
CGGCGCG267200.079.065526
CTCGAGG285850.078.252065
TCGAGGG287650.077.6556
AGGGCGC298950.074.870079
CGAGGGC314350.071.0656747
GAGGGCG322350.069.331458
CTCGACG19300.065.988355
TCTCGAC23650.054.051484
CGCGCCG565100.039.4481939
GGCGCGC564250.039.2884867
GCGCGCC569700.039.0293438
Produced by FastQC (version 0.11.5)