##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048275_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6584975 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.044008063811937 31.0 31.0 34.0 28.0 34.0 2 31.242669410286297 33.0 31.0 34.0 28.0 34.0 3 31.321481706460542 33.0 31.0 34.0 28.0 34.0 4 34.797611228592366 37.0 35.0 37.0 32.0 37.0 5 34.73363209427522 37.0 35.0 37.0 32.0 37.0 6 34.70813693294204 37.0 35.0 37.0 32.0 37.0 7 34.60814551308091 37.0 35.0 37.0 32.0 37.0 8 34.403680803647696 37.0 35.0 37.0 31.0 37.0 9 36.013157832793596 39.0 37.0 39.0 31.0 39.0 10-11 35.765367750067384 39.0 37.0 39.0 30.0 39.0 12-13 35.82834338778811 39.0 37.0 39.0 30.0 39.0 14-15 37.09264780807824 40.0 38.0 41.0 31.0 41.0 16-17 37.06827808761613 40.0 37.5 41.0 31.0 41.0 18-19 37.06347525996682 40.0 38.0 41.0 30.5 41.0 20-21 37.0842502059613 40.0 38.0 41.0 31.0 41.0 22-23 37.133661707143915 40.0 38.0 41.0 31.0 41.0 24-25 37.19418213432853 40.0 38.0 41.0 31.0 41.0 26-27 37.13061894692083 40.0 38.0 41.0 31.0 41.0 28-29 37.025937532033154 40.0 37.5 41.0 31.0 41.0 30-31 36.8201052395795 40.0 36.5 41.0 30.5 41.0 32-33 36.55251766331687 40.0 35.5 41.0 30.0 41.0 34-35 36.43164993033383 40.0 35.0 41.0 30.0 41.0 36-37 36.32911977646081 40.0 35.0 41.0 30.0 41.0 38-39 36.19878746692281 40.0 35.0 41.0 30.0 41.0 40-41 35.77350149393126 40.0 35.0 41.0 27.5 41.0 42-43 35.57032919639027 39.5 35.0 41.0 27.0 41.0 44-45 35.336853442875636 39.0 35.0 41.0 25.5 41.0 46-47 34.98138519888079 39.0 35.0 41.0 23.5 41.0 48-49 34.44360889752808 39.0 34.0 41.0 19.0 41.0 50-51 34.10042657717 39.0 34.0 41.0 14.0 41.0 52-53 33.89311409686445 39.0 34.0 41.0 10.0 41.0 54-55 33.68181170012035 39.0 34.0 40.5 7.5 41.0 56-57 33.455081074719345 38.0 33.0 40.0 7.0 41.0 58-59 33.2313567629338 38.0 33.0 40.0 3.5 41.0 60-61 32.98138314875911 37.5 33.0 40.0 2.0 41.0 62-63 32.694014404003056 37.0 33.0 40.0 2.0 41.0 64-65 32.39416740382461 36.5 32.0 40.0 2.0 41.0 66-67 32.08140380183676 36.0 32.0 39.0 2.0 41.0 68-69 31.731652815690264 35.5 32.0 39.0 2.0 41.0 70-71 31.411502929016436 35.0 31.5 39.0 2.0 40.5 72-73 31.075182897429375 35.0 31.0 37.5 2.0 40.0 74-75 30.734384337070377 35.0 31.0 37.0 2.0 39.5 76-77 30.42528733973933 35.0 31.0 37.0 2.0 39.0 78-79 30.12377040155809 35.0 31.0 36.0 2.0 39.0 80-81 29.818460358619433 35.0 30.0 36.0 2.0 37.5 82-83 29.58216447290992 35.0 30.0 35.0 2.0 37.0 84-85 29.356609706187193 34.5 30.0 35.0 2.0 37.0 86-87 29.117375844251498 34.0 30.0 35.0 2.0 36.0 88-89 28.91482572067472 34.0 30.0 35.0 2.0 36.0 90-91 28.731632770056073 34.0 30.0 35.0 2.0 36.0 92-93 28.533611137475845 34.0 29.5 35.0 2.0 35.5 94-95 28.3376745545731 34.0 29.0 35.0 2.0 35.0 96-97 28.13473984335552 34.0 29.0 35.0 2.0 35.0 98-99 27.86264442917399 34.0 29.0 35.0 2.0 35.0 100-101 26.62720891119556 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7070616189777681 1101 2 0.7422165875210531 1101 3 0.72315737309318 1101 4 0.6802379251768116 1101 5 0.7277665770406117 1101 6 0.6870920185713132 1101 7 0.6968492328613536 1101 8 0.8360635630647124 1101 9 0.8826123572637812 1101 10-11 0.998201922609752 1101 12-13 0.950656580957471 1101 14-15 1.003288788806664 1101 16-17 0.9627088203792624 1101 18-19 0.9785894242601074 1101 20-21 0.9855583525217568 1101 22-23 0.9855861562031052 1101 24-25 0.9317663403658258 1101 26-27 0.9737295195079696 1101 28-29 1.0369872541565002 1101 30-31 0.9962311028399427 1101 32-33 1.0044677427705366 1101 34-35 1.0296033525230683 1101 36-37 0.9334053681232746 1101 38-39 1.0011151676960068 1101 40-41 1.0079375054882078 1101 42-43 0.9147068613779226 1101 44-45 0.8325370037643793 1101 46-47 0.8124827493458042 1101 48-49 0.6723004936201065 1101 50-51 0.5588609074000814 1101 52-53 0.49140434313054726 1101 54-55 0.4557512437348663 1101 56-57 0.48782809143753525 1101 58-59 0.49653158345287807 1101 60-61 0.5575892805652813 1101 62-63 0.4930159345365652 1101 64-65 0.5138812167742657 1101 66-67 0.512050478630286 1101 68-69 0.5293767681784836 1101 70-71 0.5244879351252827 1101 72-73 0.5927763697057706 1101 74-75 0.543597167561586 1101 76-77 0.5562114469027826 1101 78-79 0.5035913847638405 1101 80-81 0.5483477048335494 1101 82-83 0.5222151421530512 1101 84-85 0.4972337440217771 1101 86-87 0.5028387988889129 1101 88-89 0.5572501479412928 1101 90-91 0.5387536099428267 1101 92-93 0.5679010851581907 1101 94-95 0.5810338493485325 1101 96-97 0.5909096221718926 1101 98-99 0.642328791370776 1101 100-101 0.671395611882847 1102 1 0.5902336568397608 1102 2 0.6223031137092683 1102 3 0.6252387375298696 1102 4 0.6363529637194958 1102 5 0.6044235004051473 1102 6 0.6305691183802793 1102 7 0.6314349964962602 1102 8 0.8248824397251298 1102 9 0.8349718862925926 1102 10-11 0.999305163486703 1102 12-13 0.8908211309667564 1102 14-15 1.0271795731338358 1102 16-17 1.0007541273791958 1102 18-19 1.0387809328338307 1102 20-21 1.0031637984394308 1102 22-23 0.9790068608560034 1102 24-25 0.9509056214420397 1102 26-27 0.9754170167820106 1102 28-29 1.020987468582156 1102 30-31 0.9826875985993127 1102 32-33 1.011555866748317 1102 34-35 0.9511311507154687 1102 36-37 0.9153455714713772 1102 38-39 0.9428214579572582 1102 40-41 1.0089448010385382 1102 42-43 0.8492874647435045 1102 44-45 0.7978068610091 1102 46-47 0.6953876677878839 1102 48-49 0.502116993619687 1102 50-51 0.38550159101280457 1102 52-53 0.32154441671983847 1102 54-55 0.33709728049844045 1102 56-57 0.34021619271287307 1102 58-59 0.3238682515279123 1102 60-61 0.34761245291934273 1102 62-63 0.2969855856202912 1102 64-65 0.3225675392910432 1102 66-67 0.32524258774407144 1102 68-69 0.35663105722004573 1102 70-71 0.358301221539687 1102 72-73 0.33959977104403904 1102 74-75 0.3576155510759307 1102 76-77 0.3197016741075167 1102 78-79 0.3571026406150146 1102 80-81 0.3828390794571064 1102 82-83 0.3315332202480761 1102 84-85 0.37778351417432177 1102 86-87 0.4459504116440769 1102 88-89 0.3959701779960163 1102 90-91 0.4050790153732464 1102 92-93 0.41245380467913506 1102 94-95 0.5291788862385545 1102 96-97 0.5476181808930285 1102 98-99 0.6085342424981235 1102 100-101 0.616166827637084 1103 1 0.6089565816653568 1103 2 0.6351148852952697 1103 3 0.5854765140327061 1103 4 0.6197676438340167 1103 5 0.6244213662647198 1103 6 0.5809877702132837 1103 7 0.6062715407408703 1103 8 0.7512227214871032 1103 9 0.7538101101334362 1103 10-11 0.8994800521372355 1103 12-13 0.8134789811390135 1103 14-15 0.8886471827025488 1103 16-17 0.873633498556103 1103 18-19 0.8549832853772017 1103 20-21 0.8605335812282817 1103 22-23 0.8626660663460157 1103 24-25 0.8259402665788329 1103 26-27 0.9221968898976627 1103 28-29 0.8944062645389295 1103 30-31 0.8895829508849147 1103 32-33 0.9215228568619764 1103 34-35 0.9275416235245828 1103 36-37 0.9241024762471426 1103 38-39 0.9050665736858718 1103 40-41 0.8270706515766477 1103 42-43 0.7151532744280686 1103 44-45 0.6508151675270994 1103 46-47 0.5705605470204773 1103 48-49 0.39563100827042064 1103 50-51 0.33744636112152904 1103 52-53 0.3506352451820547 1103 54-55 0.2342289403330824 1103 56-57 0.3111745603448952 1103 58-59 0.26018356107528007 1103 60-61 0.2771920549714437 1103 62-63 0.28393580194452284 1103 64-65 0.2828714880051564 1103 66-67 0.2610409918504857 1103 68-69 0.27157586058810423 1103 70-71 0.2868726501111176 1103 72-73 0.288354416956885 1103 74-75 0.27734658009192614 1103 76-77 0.2613741611571676 1103 78-79 0.2408844898892717 1103 80-81 0.28601354405116197 1103 82-83 0.22323460134180806 1103 84-85 0.21291839462088546 1103 86-87 0.21033541889183738 1103 88-89 0.22081406700963058 1103 90-91 0.209980470746153 1103 92-93 0.3060589360396371 1103 94-95 0.2731800808028737 1103 96-97 0.3090826031091538 1103 98-99 0.37449525701859443 1103 100-101 0.4442638949955473 1104 1 0.40145565037546405 1104 2 0.4814461296433592 1104 3 0.4890616839486839 1104 4 0.4953565283790766 1104 5 0.43688755353099396 1104 6 0.45015174173358474 1104 7 0.463155648788927 1104 8 0.5853669022744938 1104 9 0.6001627445320565 1104 10-11 0.7553288817394872 1104 12-13 0.6754544148305115 1104 14-15 0.7119039923074055 1104 16-17 0.6750176651799933 1104 18-19 0.7487551937251169 1104 20-21 0.7980820379196842 1104 22-23 0.6602923301689216 1104 24-25 0.6513620783753495 1104 26-27 0.7352759843811327 1104 28-29 0.7321536816080538 1104 30-31 0.705756198447439 1104 32-33 0.674584924865222 1104 34-35 0.6414702649996897 1104 36-37 0.6113026041925451 1104 38-39 0.6131220009011216 1104 40-41 0.6526688490206425 1104 42-43 0.5928202846518573 1104 44-45 0.532437362633317 1104 46-47 0.4748844963741021 1104 48-49 0.40903072315749966 1104 50-51 0.29237867642009263 1104 52-53 0.20546327575732448 1104 54-55 0.25752336351911254 1104 56-57 0.23558277991606502 1104 58-59 0.2692164148765883 1104 60-61 0.2359362418138673 1104 62-63 0.2659396942755734 1104 64-65 0.22027646681912927 1104 66-67 0.22146182957700944 1104 68-69 0.29159892235335505 1104 70-71 0.2378450089005888 1104 72-73 0.2203582434723863 1104 74-75 0.2051187964565635 1104 76-77 0.23050782462124886 1104 78-79 0.21302691076911984 1104 80-81 0.2713231864226806 1104 82-83 0.21155168481828923 1104 84-85 0.21542728300957847 1104 86-87 0.2870254961461214 1104 88-89 0.2794270834806234 1104 90-91 0.3043286928709712 1104 92-93 0.3162361575444699 1104 94-95 0.3864481317019788 1104 96-97 0.3519559977035911 1104 98-99 0.41294008845841645 1104 100-101 0.4631684216567642 1105 1 0.306610123963992 1105 2 0.36778770674887085 1105 3 0.3710837356204557 1105 4 0.35812553958742654 1105 5 0.3289285350999833 1105 6 0.398520750420424 1105 7 0.34953564887182154 1105 8 0.5382864129832754 1105 9 0.500119275075285 1105 10-11 0.6634927646048681 1105 12-13 0.5617169869978511 1105 14-15 0.5593191563533679 1105 16-17 0.5406801297837802 1105 18-19 0.5322730271993521 1105 20-21 0.5293135362545058 1105 22-23 0.4698386539807089 1105 24-25 0.5335829181554672 1105 26-27 0.5850768019985395 1105 28-29 0.6075480929513617 1105 30-31 0.6028275363044528 1105 32-33 0.6413314889268449 1105 34-35 0.5961601074642147 1105 36-37 0.6277203221773391 1105 38-39 0.5632243730824129 1105 40-41 0.5790648849239091 1105 42-43 0.4572097976312719 1105 44-45 0.4003243883195253 1105 46-47 0.38841184819426644 1105 48-49 0.4241456562926871 1105 50-51 0.38352571416959336 1105 52-53 0.3698030432191146 1105 54-55 0.3225018483258495 1105 56-57 0.28623258831667897 1105 58-59 0.2925293543603189 1105 60-61 0.30028052235913805 1105 62-63 0.2638537064461559 1105 64-65 0.2991148226809912 1105 66-67 0.25140319447407933 1105 68-69 0.33975662313716626 1105 70-71 0.2789460666535213 1105 72-73 0.2956956691991728 1105 74-75 0.3281161041118956 1105 76-77 0.2807337333711324 1105 78-79 0.28002175330412626 1105 80-81 0.30869533347475553 1105 82-83 0.30696928410517543 1105 84-85 0.3172489697069345 1105 86-87 0.3080843761378027 1105 88-89 0.35059465212322394 1105 90-91 0.3549919631681071 1105 92-93 0.40552704106155346 1105 94-95 0.4111610853334007 1105 96-97 0.41335290799514013 1105 98-99 0.4575267383279602 1105 100-101 0.37877549217270357 1106 1 0.24769273510709766 1106 2 0.32543555374879674 1106 3 0.26515591415143547 1106 4 0.3382097969455913 1106 5 0.32600751913425086 1106 6 0.2843587594483168 1106 7 0.3485857832321244 1106 8 0.44958364982765175 1106 9 0.3539236088102413 1106 10-11 0.5020002171313678 1106 12-13 0.4153430474250399 1106 14-15 0.44090639896514716 1106 16-17 0.44352833650571455 1106 18-19 0.49239591887129563 1106 20-21 0.4412801360165233 1106 22-23 0.35331493531372615 1106 24-25 0.43794534801511276 1106 26-27 0.5034841346553662 1106 28-29 0.4325223822591511 1106 30-31 0.46391896636667695 1106 32-33 0.551688855945244 1106 34-35 0.474588096892532 1106 36-37 0.5001308482058846 1106 38-39 0.5089899680906811 1106 40-41 0.5108717485104464 1106 42-43 0.446489781174499 1106 44-45 0.3563361751723946 1106 46-47 0.33731095524551336 1106 48-49 0.24617944273555992 1106 50-51 0.23341775115216734 1106 52-53 0.2252633738668024 1106 54-55 0.20422339802634326 1106 56-57 0.22992229091576633 1106 58-59 0.21654803070983775 1106 60-61 0.21914985143146026 1106 62-63 0.22323968390831084 1106 64-65 0.18460591333322895 1106 66-67 0.1476344358511099 1106 68-69 0.19599145927653794 1106 70-71 0.22868573824857563 1106 72-73 0.18621642070046462 1106 74-75 0.16616141209613744 1106 76-77 0.1995153494523798 1106 78-79 0.165674346022346 1106 80-81 0.2029605925040947 1106 82-83 0.19635337114060292 1106 84-85 0.22050077249472722 1106 86-87 0.21703876870707006 1106 88-89 0.22850223381406565 1106 90-91 0.2424652478198155 1106 92-93 0.24144832640952174 1106 94-95 0.3353793213558056 1106 96-97 0.3672079910578816 1106 98-99 0.410872309199906 1106 100-101 0.44380027932194466 1107 1 0.2298851604834269 1107 2 0.24643046310438876 1107 3 0.30360973285192117 1107 4 0.3046812478202554 1107 5 0.23422877135595144 1107 6 0.24280878814968077 1107 7 0.2168961401112952 1107 8 0.4011365463767049 1107 9 0.2890281595389155 1107 10-11 0.5220913606479627 1107 12-13 0.36316540892273963 1107 14-15 0.4242442158859774 1107 16-17 0.3189934837325552 1107 18-19 0.4323991156088809 1107 20-21 0.42429491609011194 1107 22-23 0.31118082631569166 1107 24-25 0.3811955907489377 1107 26-27 0.42685589351997066 1107 28-29 0.4588657247131067 1107 30-31 0.43151524302248845 1107 32-33 0.43684447734707277 1107 34-35 0.38227053884527606 1107 36-37 0.34390371382576745 1107 38-39 0.3490083432846234 1107 40-41 0.39501433234288186 1107 42-43 0.32389810924455986 1107 44-45 0.25563490969525304 1107 46-47 0.1569231175186303 1107 48-49 0.13304667459134834 1107 50-51 0.10299232162875427 1107 52-53 0.07798618704426019 1107 54-55 0.0785756971593301 1107 56-57 0.019316848740558612 1107 58-59 0.03643558961335458 1107 60-61 -0.03582477968892306 1107 62-63 -0.023715434678912573 1107 64-65 0.030646057554761796 1107 66-67 0.08404039041884204 1107 68-69 0.05851064147909213 1107 70-71 0.10893157937858788 1107 72-73 0.0859264757949667 1107 74-75 0.09975404768353258 1107 76-77 0.1643965740265152 1107 78-79 0.12891710466429984 1107 80-81 0.1990386107245392 1107 82-83 0.15073265709581207 1107 84-85 0.11412156339192236 1107 86-87 0.08261234604346868 1107 88-89 0.13237481108147264 1107 90-91 0.11441693303320832 1107 92-93 0.10008880139983134 1107 94-95 0.14720990695215264 1107 96-97 0.1790341632636938 1107 98-99 0.13782875859412158 1107 100-101 0.24676254917198648 1108 1 0.19749220036603177 1108 2 0.20076863030662295 1108 3 0.18714821875822096 1108 4 0.251811145452713 1108 5 0.23585195245161827 1108 6 0.20235695531235365 1108 7 0.20457791351670096 1108 8 0.3825691989119946 1108 9 0.3321173250733338 1108 10-11 0.44281581607728526 1108 12-13 0.387031169033321 1108 14-15 0.3535628514138409 1108 16-17 0.2949808697061371 1108 18-19 0.31241699118947963 1108 20-21 0.25772428787992396 1108 22-23 0.19812374950109302 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-0.7428821763456028 2215 58-59 -0.7262687828803323 2215 60-61 -0.6827319347411844 2215 62-63 -0.6617823247364214 2215 64-65 -0.678079375585952 2215 66-67 -0.6955865508303525 2215 68-69 -0.6589785976055857 2215 70-71 -0.6256887811401413 2215 72-73 -0.7142489690068565 2215 74-75 -0.7242762873603965 2215 76-77 -0.6913659607648732 2215 78-79 -0.6667539752311527 2215 80-81 -0.570357598861964 2215 82-83 -0.6950391727929137 2215 84-85 -0.7331599702781766 2215 86-87 -0.8011584473689837 2215 88-89 -0.8495655585574262 2215 90-91 -0.884981218716689 2215 92-93 -0.7627197319554462 2215 94-95 -0.7927726422328902 2215 96-97 -0.865002075683222 2215 98-99 -0.9339675603454616 2215 100-101 -0.9474838783871782 2216 1 -0.9931134828324453 2216 2 -0.9846502639753005 2216 3 -1.1554157572603287 2216 4 -1.0754565846181166 2216 5 -1.0496303602968808 2216 6 -1.065917567886558 2216 7 -1.051134677202164 2216 8 -1.0029573086315793 2216 9 -1.2694387279761372 2216 10-11 -1.1530324931074674 2216 12-13 -1.197814208132165 2216 14-15 -1.5342629644378007 2216 16-17 -1.392721113934428 2216 18-19 -1.280721533539463 2216 20-21 -1.35110275049243 2216 22-23 -1.274930361368078 2216 24-25 -1.3673497125803067 2216 26-27 -1.6285237452907353 2216 28-29 -1.4958536262177802 2216 30-31 -1.3574069292963742 2216 32-33 -1.3453091633747718 2216 34-35 -1.3427857819508517 2216 36-37 -1.3444891222415976 2216 38-39 -1.5511645435671966 2216 40-41 -1.3849626548675786 2216 42-43 -1.3499468316144814 2216 44-45 -1.1884877090302908 2216 46-47 -1.1657204051853967 2216 48-49 -1.1786643782658572 2216 50-51 -1.0722293548459092 2216 52-53 -1.0267967228624357 2216 54-55 -1.0612046698931366 2216 56-57 -1.0673633572562267 2216 58-59 -0.966603302768533 2216 60-61 -1.0002427425564875 2216 62-63 -0.9816181568452222 2216 64-65 -0.9748558132299436 2216 66-67 -0.9456224416357486 2216 68-69 -1.0167123307700372 2216 70-71 -1.0123390413865216 2216 72-73 -0.9415416526923828 2216 74-75 -0.9406162062536012 2216 76-77 -1.0081646835052744 2216 78-79 -1.0300496505497065 2216 80-81 -1.0389666856091786 2216 82-83 -1.0640901238738998 2216 84-85 -1.110641524466704 2216 86-87 -1.0803819650939346 2216 88-89 -1.121638961763157 2216 90-91 -1.2002740998608132 2216 92-93 -1.1681715165551658 2216 94-95 -1.1916576942787849 2216 96-97 -1.2519206632321058 2216 98-99 -1.314550262701644 2216 100-101 -1.4719624896310926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126515.0 3 25346.0 4 15191.0 5 10607.0 6 9648.0 7 10621.0 8 12995.0 9 14423.0 10 15948.0 11 17237.0 12 19378.0 13 24691.0 14 34064.0 15 53440.0 16 59165.0 17 85474.0 18 131156.0 19 60708.0 20 37856.0 21 32816.0 22 33813.0 23 38012.0 24 43561.0 25 50357.0 26 59066.0 27 70660.0 28 84331.0 29 97933.0 30 113071.0 31 135942.0 32 170452.0 33 222218.0 34 302610.0 35 456855.0 36 795628.0 37 1352912.0 38 1430180.0 39 329913.0 40 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.690997627896483 38.20642237722294 16.64633326573256 19.45624672914801 2 22.536581556106814 27.780543179622576 32.9102420455951 16.772633218675516 3 21.59479189614178 30.302048753807703 23.028114341276655 25.07504500877386 4 24.849621992249908 25.881336837924216 27.741380520710727 21.527660649115145 5 21.97872143218558 29.01253101605009 24.340332489018078 24.66841506274625 6 22.046247870187855 25.020193155621413 28.33281236662569 24.600746607565043 7 25.19094600568946 25.773211533289096 23.927059696530602 25.10878276449084 8 28.57176053934676 25.089985545509318 25.243339535636768 21.094914379507156 9 21.117612182203324 28.605588592457863 25.408638031174767 24.86816119416404 10-11 26.876810845254894 25.427435990063664 24.93964830046622 22.756104864215228 12-13 25.016872023000175 25.51283014165361 24.77033203123665 24.699965804109567 14-15 24.820581089070227 25.49642111434322 24.991340885109246 24.691656911477306 16-17 24.87726446651099 27.08666734457543 24.9140279802159 23.12204020869768 18-19 23.082491525597497 25.32720667669381 26.794839908122935 24.79546188958576 20-21 22.885892084985805 28.825201869611135 25.49164779340411 22.79725825199895 22-23 21.102007834488457 27.007310467806146 29.04226492357652 22.84841677412887 24-25 21.043979644780027 25.247985073810757 30.91245505678732 22.795580224621894 26-27 20.991757452745954 25.2144776983745 33.01556243830923 20.778202410570316 28-29 21.074871770570816 25.20404088821899 33.03376302019852 20.687324321011673 30-31 21.13654493286714 25.040680657314677 32.953728196485166 20.86904621333302 32-33 21.107469296873337 24.9642923320403 33.08717926130236 20.84105910978399 34-35 21.21003771970412 25.134767199994474 32.94349243407481 20.7117026462266 36-37 21.173285483705843 25.022860521049754 32.85898385059882 20.94487014464558 38-39 21.280079491417776 24.98561792925829 32.78370049199062 20.950602087333312 40-41 21.769538660473504 25.573423484188357 31.38533869766164 21.271699157676494 42-43 22.094835758992414 25.55708832190291 30.88754261009845 21.460533309006227 44-45 22.22917229223563 25.895011406849882 30.371509726110276 21.50430657480421 46-47 22.47004688241403 26.32724446053702 29.54097951986751 21.66172913718144 48-49 22.693890933128756 26.764442264101984 28.311909772108045 22.22975703066122 50-51 23.01690772855938 26.92651137975493 27.52657721989041 22.530003671795278 52-53 23.127702173345327 27.038824398212636 27.15660759479056 22.676865833651476 54-55 23.26021754687711 26.767412638625736 27.034119518595105 22.93825029590204 56-57 23.20873250334989 26.59148972601309 27.398675937187804 22.80110183344922 58-59 23.23163960951032 26.849412971770732 27.22676194500974 22.69218547370921 60-61 23.238457608044484 26.824929278497095 27.09619233513978 22.840420778318645 62-63 23.263661771956492 26.868912474863617 27.175084459010318 22.692341294169573 64-65 23.343589955519523 27.04623714858205 26.99697224026872 22.613200655629704 66-67 23.36357631427988 26.925046608686355 26.90253983918484 22.808837237848927 68-69 23.408430137065167 26.91026397924423 27.017349820253923 22.663956063436682 70-71 23.444417187605616 27.08194696865556 26.865974072923603 22.607661770815216 72-73 23.445089093465164 27.045412945419855 26.74531823543217 22.764179725682812 74-75 23.424192256333676 27.028322533745598 26.901603050078123 22.64588215984261 76-77 23.496991263468832 27.21894129295314 26.679086736738363 22.60498070683966 78-79 23.462267787527605 27.174978494889096 26.633767486550997 22.7289862310323 80-81 23.40783609357301 27.10523739271456 26.80316674012908 22.683759773583354 82-83 23.40066112322274 27.259740415620037 26.661221371749406 22.67837708940782 84-85 23.441538344900284 27.06139951718573 26.69737445241268 22.799687685501304 86-87 23.379994987758444 26.96193970557525 26.877115987508443 22.780949319157862 88-89 23.43304449398214 27.03583579063543 26.70790598965867 22.82321372572376 90-91 23.466751260224473 26.961363092702793 26.61790686702031 22.95397878005242 92-93 23.467970514544668 26.97362389371624 26.792600303756853 22.765805287982236 94-95 23.534981523110616 27.17713751162496 26.609804494063184 22.67807647120124 96-97 23.527000394286095 27.160516520101265 26.41382630918972 22.89865677642292 98-99 23.48732295866543 27.117975707850146 26.590077680134776 22.804623653349644 100-101 23.725989222136203 27.184116941029806 26.19717522538948 22.89271861144451 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 472.0 1 405.5 2 342.0 3 327.5 4 336.0 5 378.0 6 365.5 7 340.5 8 364.5 9 376.0 10 376.5 11 407.0 12 418.0 13 444.0 14 476.5 15 562.0 16 1218.5 17 1466.5 18 1336.5 19 1948.5 20 3068.0 21 4810.0 22 7458.0 23 11062.5 24 15882.0 25 22137.5 26 30006.0 27 39506.0 28 50222.0 29 62243.5 30 75769.0 31 91062.0 32 108588.0 33 125891.0 34 139431.5 35 153239.5 36 169556.5 37 188182.0 38 202318.5 39 211237.0 40 220177.0 41 230739.0 42 245513.5 43 258176.0 44 265174.0 45 266317.5 46 265453.5 47 264398.0 48 263977.5 49 259378.5 50 248841.0 51 236655.0 52 215409.5 53 189502.0 54 171088.0 55 157813.0 56 143661.0 57 128662.0 58 117195.5 59 108613.5 60 96712.0 61 84067.5 62 74251.5 63 65274.0 64 56627.0 65 48220.5 66 40588.5 67 33575.5 68 26951.0 69 21229.0 70 16224.0 71 12097.5 72 8828.0 73 6378.0 74 4477.0 75 2988.5 76 1963.0 77 1314.5 78 867.5 79 534.5 80 342.0 81 209.5 82 135.0 83 106.5 84 88.0 85 66.5 86 50.5 87 47.0 88 44.5 89 31.5 90 25.5 91 19.5 92 12.0 93 11.5 94 12.0 95 10.5 96 7.0 97 7.0 98 6.5 99 7.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033561251181667355 2 0.055474774012050164 3 0.04441930303455974 4 0.05911943477386019 5 0.05626445051044233 6 0.01611243778450184 7 0.007927137156936815 8 0.013090406569501025 9 0.00636297024666001 10-11 0.013067627439739712 12-13 0.012915766574664292 14-15 0.013249860477830212 16-17 0.015208865637303103 18-19 0.01788161686263046 20-21 0.01740325513764289 22-23 0.019210399432040365 24-25 0.016871742109878927 26-27 0.010994726631460255 28-29 0.013333383953621691 30-31 0.006947634577200369 32-33 0.014502712614702411 34-35 0.011397157923910113 36-37 0.023887714076363236 38-39 0.03228561991503385 40-41 0.033880158998325735 42-43 0.03748685454386691 44-45 0.03504189461615268 46-47 0.03385737986856442 48-49 0.044502826510351215 50-51 0.03589990850382879 52-53 0.03860303190217123 54-55 0.0517086245581798 56-57 0.046112551680150646 58-59 0.04763116033090483 60-61 0.04817026640192256 62-63 0.05132897239549125 64-65 0.04876252377571669 66-67 0.03813226322043744 68-69 0.02943822869486976 70-71 0.022930990626388103 72-73 0.02612006879297188 74-75 0.029316740002809424 76-77 0.02753237483817327 78-79 0.03397886856062476 80-81 0.034069985079670004 82-83 0.04777542815272647 84-85 0.047805800325741554 86-87 0.04691741426505036 88-89 0.04906624550586752 90-91 0.044244663039723006 92-93 0.03656050326690686 94-95 0.03275638859676764 96-97 0.03328030858127783 98-99 0.025755602716790876 100-101 0.02671991921001978 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6584975.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.253627065052456 #Duplication Level Percentage of deduplicated Percentage of total 1 80.83750666676342 43.85727939567274 2 11.418737386234735 12.390158394131022 3 3.065542707872822 4.989504324747694 4 1.2372836619701102 2.6850850548083516 5 0.6788069710735009 1.8413870128889782 6 0.4271547652305325 1.3904817199126407 7 0.32387679137326875 1.2300043457933785 8 0.23314078308292963 1.0118986479228442 9 0.18262837805132429 0.8917426722862763 >10 1.3554049267856685 14.130062704210475 >50 0.158890360795626 6.126482354391671 >100 0.0794644898836683 6.537821480466565 >500 6.816578342339859E-4 0.2673812190660017 >1k 6.816410696991288E-4 0.7738070772859176 >5k 8.520513362113474E-5 0.4000627889737478 >10k+ 1.1360684482817966E-4 1.476840807441779 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 59997 0.9111196321929848 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 12327 0.18719888837846765 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 12051 0.1830075285023861 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 11468 0.17415404006848925 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 8908 0.13527765860918226 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 8580 0.13029662223470856 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 8475 0.12870208315141668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.518608650754179E-5 0.0 0.0 0.0 0.0 2 1.518608650754179E-5 0.0 0.0 3.037217301508358E-5 0.0 3 1.518608650754179E-5 0.0 1.518608650754179E-5 3.037217301508358E-5 0.0 4 1.518608650754179E-5 0.0 1.518608650754179E-5 3.037217301508358E-5 0.0 5 6.074434603016716E-5 0.0 1.518608650754179E-5 3.037217301508358E-5 0.0 6 6.074434603016716E-5 0.0 1.518608650754179E-5 3.037217301508358E-5 0.0 7 9.111651904525074E-5 0.0 1.518608650754179E-5 3.037217301508358E-5 0.0 8 1.518608650754179E-4 0.0 1.518608650754179E-5 3.037217301508358E-5 0.0 9 7.137460658544642E-4 0.0 1.518608650754179E-5 3.037217301508358E-5 0.0 10-11 9.870956229902164E-4 0.0 1.518608650754179E-5 3.7965216268854475E-5 0.0 12-13 0.0014806434344853245 0.0 1.518608650754179E-5 4.555825952262537E-5 0.0 14-15 0.0017539929916210767 7.593043253770895E-6 1.518608650754179E-5 4.555825952262537E-5 0.0 16-17 0.0020653077650256836 1.518608650754179E-5 1.518608650754179E-5 7.593043253770895E-5 0.0 18-19 0.0026195999225509586 1.518608650754179E-5 1.518608650754179E-5 7.593043253770895E-5 0.0 20-21 0.0035839164157798623 1.518608650754179E-5 2.2779129761312684E-5 7.593043253770895E-5 0.0 22-23 0.005497363315730128 1.518608650754179E-5 3.037217301508358E-5 9.111651904525074E-5 0.0 24-25 0.006970413706961682 1.518608650754179E-5 3.7965216268854475E-5 9.111651904525074E-5 0.0 26-27 0.007615822383532207 1.518608650754179E-5 5.315130277639626E-5 9.870956229902163E-5 0.0 28-29 0.008716813655328988 1.518608650754179E-5 9.870956229902163E-5 1.1389564880656343E-4 0.0 30-31 0.010083561441007749 1.518608650754179E-5 1.3667477856787612E-4 1.2148869206033432E-4 0.0 32-33 0.011435123140178968 1.518608650754179E-5 1.8223303809050147E-4 1.2908173531410522E-4 0.0 34-35 0.013804152635355487 1.518608650754179E-5 1.898260813442724E-4 1.3667477856787612E-4 0.0 36-37 0.017418441224150434 2.2779129761312684E-5 1.9741912459804328E-4 1.518608650754179E-4 0.0 38-39 0.02181481326808378 3.037217301508358E-5 1.9741912459804328E-4 1.670469515829597E-4 0.0 40-41 0.027532374838173265 3.037217301508358E-5 1.9741912459804328E-4 1.7463999483673058E-4 0.0 42-43 0.034457230285612324 3.037217301508358E-5 1.9741912459804328E-4 1.898260813442724E-4 0.0 44-45 0.042596972653654724 3.037217301508358E-5 1.9741912459804328E-4 1.9741912459804328E-4 0.0 46-47 0.05252108018633328 3.037217301508358E-5 1.9741912459804328E-4 1.9741912459804328E-4 0.0 48-49 0.06369044681263027 4.555825952262537E-5 1.9741912459804328E-4 2.1260521110558507E-4 0.0 50-51 0.07788184465392808 4.555825952262537E-5 1.9741912459804328E-4 2.1260521110558507E-4 0.0 52-53 0.09413855026025156 4.555825952262537E-5 1.9741912459804328E-4 2.1260521110558507E-4 0.0 54-55 0.11148106105186428 4.555825952262537E-5 1.9741912459804328E-4 2.1260521110558507E-4 0.0 56-57 0.13107111264659319 4.555825952262537E-5 2.1260521110558507E-4 2.1260521110558507E-4 0.0 58-59 0.15399451022972752 4.555825952262537E-5 2.1260521110558507E-4 2.2779129761312685E-4 0.0 60-61 0.17961343816795053 4.555825952262537E-5 2.1260521110558507E-4 2.2779129761312685E-4 0.0 62-63 0.2083151416672045 4.555825952262537E-5 2.1260521110558507E-4 2.2779129761312685E-4 0.0 64-65 0.2407146572310449 4.555825952262537E-5 2.1260521110558507E-4 2.3538434086689775E-4 0.0 66-67 0.2760374944475871 6.074434603016716E-5 2.1260521110558507E-4 2.4297738412066864E-4 0.0 68-69 0.31463293330650455 6.074434603016716E-5 2.1260521110558507E-4 2.4297738412066864E-4 0.0 70-71 0.3569109981435009 6.074434603016716E-5 2.1260521110558507E-4 2.4297738412066864E-4 0.0 72-73 0.4044966002148831 6.074434603016716E-5 2.1260521110558507E-4 2.5816347062821045E-4 0.0 74-75 0.45707083170399276 6.074434603016716E-5 2.1260521110558507E-4 2.6575651388198137E-4 0.0 76-77 0.5127658039704024 6.074434603016716E-5 2.1260521110558507E-4 3.1131477340460667E-4 0.0 78-79 0.5712626091974533 6.074434603016716E-5 2.1260521110558507E-4 3.568730329272321E-4 0.0 80-81 0.6353782664322948 6.074434603016716E-5 2.1260521110558507E-4 3.6446607618100294E-4 0.0 82-83 0.7063656278117988 6.074434603016716E-5 2.1260521110558507E-4 3.7205911943477386E-4 0.0 84-85 0.7836020637891563 6.074434603016716E-5 2.1260521110558507E-4 3.796521626885448E-4 0.0 86-87 0.8678544717329982 6.074434603016716E-5 2.1260521110558507E-4 3.796521626885448E-4 0.0 88-89 0.957877592549706 6.074434603016716E-5 2.1260521110558507E-4 3.9483824919608657E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 23140 0.0 92.82639 1 TATCTCG 27140 0.0 88.59731 2 TCGACGG 24640 0.0 86.61283 2 ATCTCGA 28910 0.0 83.025055 3 ATATCTC 29335 0.0 82.5219 1 CGACGGC 25975 0.0 82.216194 3 TCTCGAG 27680 0.0 81.6168 4 GACGGCG 26360 0.0 80.49382 4 ACGGCGC 26300 0.0 80.370895 5 CGGCGCG 26720 0.0 79.06552 6 CTCGAGG 28585 0.0 78.25206 5 TCGAGGG 28765 0.0 77.655 6 AGGGCGC 29895 0.0 74.87007 9 CGAGGGC 31435 0.0 71.065674 7 GAGGGCG 32235 0.0 69.33145 8 CTCGACG 1930 0.0 65.98835 5 TCTCGAC 2365 0.0 54.05148 4 CGCGCCG 56510 0.0 39.448193 9 GGCGCGC 56425 0.0 39.288486 7 GCGCGCC 56970 0.0 39.029343 8 >>END_MODULE