FastQCFastQC Report
Thu 26 May 2016
SRR2048275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6584975
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT387690.5887493878108877No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT301850.458392021230149No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166950.2535317142434102No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT74040.11243778450183942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG26100.083.058981
TCGACGG38700.061.8657652
TATCTCG91200.060.8385472
ATATCTC101500.055.483921
ATCTCGA190100.052.1060451
TCTCGAG202750.047.1643372
CGACGGC54100.044.423713
GACGGCG60050.040.575694
ACGGCGC60300.040.013635
CGGCGCG63500.038.0719956
CTCGAGG253200.037.8546333
TCGAGGG256150.037.418674
CGAGGGC300400.032.0016335
AGGGCGC316050.030.371917
GGGCGCG322150.029.7970338
GAGGGCG324050.029.6807276
GGCGCGC388750.025.681719
CTCGACG14350.023.8309943
TCTCGAC14950.023.513722
TATGCCG13850.018.51870246-47
Produced by FastQC (version 0.11.5)