##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048275_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6584975 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.236264830162604 34.0 31.0 34.0 30.0 34.0 2 32.395599983295305 34.0 31.0 34.0 30.0 34.0 3 32.465472837786024 34.0 31.0 34.0 30.0 34.0 4 35.93549269359413 37.0 35.0 37.0 35.0 37.0 5 35.8962967968747 37.0 35.0 37.0 35.0 37.0 6 35.82783670401179 37.0 35.0 37.0 35.0 37.0 7 35.7502307905497 37.0 35.0 37.0 33.0 37.0 8 35.710761696134 37.0 35.0 37.0 33.0 37.0 9 37.33112365650591 39.0 37.0 39.0 34.0 39.0 10-11 37.325538062027576 39.0 37.0 39.0 34.0 39.0 12-13 37.346629941647464 39.0 37.0 39.0 34.0 39.0 14-15 38.739270612265045 40.0 38.0 41.0 34.0 41.0 16-17 38.79211371341577 40.0 38.0 41.0 34.5 41.0 18-19 38.88518331504676 40.0 38.0 41.0 35.0 41.0 20-21 38.96822630306114 40.0 38.0 41.0 35.0 41.0 22-23 38.985668434580234 40.0 38.0 41.0 36.0 41.0 24-25 38.9964183614972 40.0 38.0 41.0 36.0 41.0 26-27 38.88678339705162 40.0 38.0 41.0 35.0 41.0 28-29 38.737968025694855 40.0 38.0 41.0 35.0 41.0 30-31 38.60716464375339 40.0 38.0 41.0 35.0 41.0 32-33 38.493287218250636 40.0 38.0 41.0 35.0 41.0 34-35 38.42995334074921 40.0 38.0 41.0 35.0 41.0 36-37 38.35752914779479 40.0 38.0 41.0 34.5 41.0 38-39 38.24442021419975 40.0 38.0 41.0 34.0 41.0 40-41 38.09262639569626 40.0 37.5 41.0 34.0 41.0 42-43 37.88858378353753 40.0 37.0 41.0 33.0 41.0 44-45 37.645659095137034 40.0 37.0 41.0 33.0 41.0 46-47 37.330525780280105 40.0 37.0 41.0 33.0 41.0 48-49 37.039441000155655 40.0 37.0 41.0 32.0 41.0 50-51 36.788392211056234 40.0 36.0 41.0 31.0 41.0 52-53 36.58683032205893 40.0 36.0 41.0 31.0 41.0 54-55 36.356776904999634 39.0 35.5 41.0 31.0 41.0 56-57 36.114509850075365 39.0 35.0 41.0 30.0 41.0 58-59 35.86949449314538 39.0 35.0 41.0 30.0 41.0 60-61 35.60613282510563 38.5 35.0 40.0 29.5 41.0 62-63 35.306368740959535 38.0 35.0 40.0 29.0 41.0 64-65 34.98383494242575 37.0 35.0 40.0 29.0 41.0 66-67 34.65363308137084 37.0 34.0 40.0 28.0 41.0 68-69 34.29249215069154 36.0 34.0 39.0 28.0 41.0 70-71 33.950435498995816 36.0 34.0 39.0 27.5 41.0 72-73 33.58827550598142 35.0 34.0 38.5 27.0 40.0 74-75 33.25423148910968 35.0 34.0 37.0 26.0 40.0 76-77 32.951009836787534 35.0 33.0 37.0 26.0 39.0 78-79 32.661445487644215 35.0 33.0 36.5 26.0 39.0 80-81 32.40404071389793 35.0 33.0 36.0 26.0 38.5 82-83 32.165185820751034 35.0 33.0 36.0 26.0 37.0 84-85 31.926388331011125 35.0 33.0 35.0 25.5 37.0 86-87 31.75623605252867 35.0 33.0 35.0 25.0 36.5 88-89 31.612008853488433 35.0 33.0 35.0 25.0 36.0 90-91 31.460593851912876 35.0 33.0 35.0 25.0 36.0 92-93 31.272131860789145 35.0 33.0 35.0 24.5 36.0 94-95 31.146191595260422 35.0 33.0 35.0 24.0 35.5 96-97 31.031617963621727 35.0 33.0 35.0 23.5 35.0 98-99 30.891503612390327 35.0 33.0 35.0 21.5 35.0 100-101 30.02894513646597 34.5 31.5 35.0 10.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45244352581358527 1101 2 0.4142929194198999 1101 3 0.38770495950814166 1101 4 0.2998510829978258 1101 5 0.31299909586989116 1101 6 0.2734678813833398 1101 7 0.2395553820103018 1101 8 0.24822517038984415 1101 9 0.3140020216975188 1101 10-11 0.2819567025765508 1101 12-13 0.30958713955782713 1101 14-15 0.4413582678899317 1101 16-17 0.46722482119321995 1101 18-19 0.4859663827975709 1101 20-21 0.4509978550075786 1101 22-23 0.45377000995357974 1101 24-25 0.46980088790000707 1101 26-27 0.5100778939462742 1101 28-29 0.5268419302927896 1101 30-31 0.5263293193211922 1101 32-33 0.5491979594377554 1101 34-35 0.5604400945400414 1101 36-37 0.5316843070785708 1101 38-39 0.5346129750465423 1101 40-41 0.5518641986285147 1101 42-43 0.5694388990592785 1101 44-45 0.5315590320677188 1101 46-47 0.5260046979297002 1101 48-49 0.4938037515802165 1101 50-51 0.47461775421751895 1101 52-53 0.49690188096584365 1101 54-55 0.48381061993634233 1101 56-57 0.45149068754379584 1101 58-59 0.46656326606600373 1101 60-61 0.48466073857166947 1101 62-63 0.5089900029723609 1101 64-65 0.4867557751865803 1101 66-67 0.4485065212992936 1101 68-69 0.4280565906403382 1101 70-71 0.4110459925338219 1101 72-73 0.39618340892585735 1101 74-75 0.3877619102025136 1101 76-77 0.3716441928672438 1101 78-79 0.43394521408811926 1101 80-81 0.40366777112804186 1101 82-83 0.4350328850194032 1101 84-85 0.43535404546317125 1101 86-87 0.4801041196417586 1101 88-89 0.4779755297134969 1101 90-91 0.44190499963555396 1101 92-93 0.4664422603526752 1101 94-95 0.42365340550449204 1101 96-97 0.4462626538868406 1101 98-99 0.48876706554473515 1101 100-101 0.6787912753836487 1102 1 0.44981383335493774 1102 2 0.4249907341309367 1102 3 0.36680986248588 1102 4 0.28113209625074376 1102 5 0.2821556171863193 1102 6 0.25196428430507467 1102 7 0.19996876020613996 1102 8 0.2088085080943074 1102 9 0.29136626702034363 1102 10-11 0.2738452669997855 1102 12-13 0.26656504786846114 1102 14-15 0.3759872625045091 1102 16-17 0.42260627804539297 1102 18-19 0.40510332940089455 1102 20-21 0.4342887382637102 1102 22-23 0.4195975811462844 1102 24-25 0.43302419056733754 1102 26-27 0.45111356191993934 1102 28-29 0.4497452010169596 1102 30-31 0.46228281508388136 1102 32-33 0.4945714856696455 1102 34-35 0.5369972860930972 1102 36-37 0.5821587471804293 1102 38-39 0.5647896124377354 1102 40-41 0.5161225950523161 1102 42-43 0.5226608106497537 1102 44-45 0.5286296322649591 1102 46-47 0.5540372983154 1102 48-49 0.5537564810429103 1102 50-51 0.5931471985083903 1102 52-53 0.6001903257202343 1102 54-55 0.6016932327025657 1102 56-57 0.6217447981443414 1102 58-59 0.6188044890586113 1102 60-61 0.6406664123426893 1102 62-63 0.6874779683047905 1102 64-65 0.6727993286281873 1102 66-67 0.5999380280427644 1102 68-69 0.6007282599685198 1102 70-71 0.5800703464523238 1102 72-73 0.5641849796240024 1102 74-75 0.5601635986316467 1102 76-77 0.5391191690606902 1102 78-79 0.5282414366584405 1102 80-81 0.5453788401280661 1102 82-83 0.5364628368200215 1102 84-85 0.5446106985119386 1102 86-87 0.5259534281944909 1102 88-89 0.5808838369154117 1102 90-91 0.6002549041582164 1102 92-93 0.6205915684085923 1102 94-95 0.6046121791522125 1102 96-97 0.5546189497324363 1102 98-99 0.5196264916563607 1102 100-101 0.6033245461789107 1103 1 0.38474680194426725 1103 2 0.3284097933907475 1103 3 0.35542962795216226 1103 4 0.25049886629849993 1103 5 0.2768105654819166 1103 6 0.20669486986331265 1103 7 0.22259480614282268 1103 8 0.2090413234135582 1103 9 0.2613420985729178 1103 10-11 0.2439184513867403 1103 12-13 0.23312140441957752 1103 14-15 0.31262996859457104 1103 16-17 0.37372510809298376 1103 18-19 0.4120084516150726 1103 20-21 0.3892265017399765 1103 22-23 0.42235879854867164 1103 24-25 0.4211914905471019 1103 26-27 0.41180345745792124 1103 28-29 0.3917090536670429 1103 30-31 0.35451114870824085 1103 32-33 0.4048745336102044 1103 34-35 0.42940057765825657 1103 36-37 0.4536744857731705 1103 38-39 0.41184068768420445 1103 40-41 0.40410170264902234 1103 42-43 0.40220036292372185 1103 44-45 0.369361088109045 1103 46-47 0.34878876796209823 1103 48-49 0.32889118133476813 1103 50-51 0.3727071221227902 1103 52-53 0.33275238901828885 1103 54-55 0.3116995796916271 1103 56-57 0.3564004670587835 1103 58-59 0.32488069172988787 1103 60-61 0.33733507456923206 1103 62-63 0.38079367419252463 1103 64-65 0.34523752863317725 1103 66-67 0.3235384338066041 1103 68-69 0.3315708851275474 1103 70-71 0.2851667846550967 1103 72-73 0.27911613280816283 1103 74-75 0.2744143213292034 1103 76-77 0.30202760057834865 1103 78-79 0.28530558005050466 1103 80-81 0.24617304524389994 1103 82-83 0.2860935196618115 1103 84-85 0.29317777565418623 1103 86-87 0.28153718489139834 1103 88-89 0.2991814580417369 1103 90-91 0.2795306683606462 1103 92-93 0.32399351297083356 1103 94-95 0.3010934111887096 1103 96-97 0.3708504041907119 1103 98-99 0.36493526297351053 1103 100-101 0.502382475619239 1104 1 0.35918305893552116 1104 2 0.35587922536314665 1104 3 0.32309511125541945 1104 4 0.2138521154142694 1104 5 0.19457207365818618 1104 6 0.15437316087067643 1104 7 0.16183850660839738 1104 8 0.14766438333597165 1104 9 0.12723075974003706 1104 10-11 0.13020943636895055 1104 12-13 0.18700741716936875 1104 14-15 0.2443103117304659 1104 16-17 0.31493225065865715 1104 18-19 0.2601645963513448 1104 20-21 0.29440078787171586 1104 22-23 0.32089525838343036 1104 24-25 0.31424716127985874 1104 26-27 0.27963548698541274 1104 28-29 0.3563549199904088 1104 30-31 0.330221100939319 1104 32-33 0.30008072711578393 1104 34-35 0.35540712762080773 1104 36-37 0.34590877856545177 1104 38-39 0.27600909517190075 1104 40-41 0.28971988827254336 1104 42-43 0.26412561226589304 1104 44-45 0.31911783387282355 1104 46-47 0.3073541010742957 1104 48-49 0.2869721120718083 1104 50-51 0.37540523018927985 1104 52-53 0.37401494826872295 1104 54-55 0.3275858056837677 1104 56-57 0.38976302249191974 1104 58-59 0.44624948384361574 1104 60-61 0.36435202470342887 1104 62-63 0.36508087214856033 1104 64-65 0.3680414615065928 1104 66-67 0.32070737834911256 1104 68-69 0.36912819400908603 1104 70-71 0.3666401708251428 1104 72-73 0.29652878571412344 1104 74-75 0.29338482806937805 1104 76-77 0.3042845799872964 1104 78-79 0.28341996482657805 1104 80-81 0.2914689453306494 1104 82-83 0.3393113072329399 1104 84-85 0.3017137221277295 1104 86-87 0.33155156558691345 1104 88-89 0.29357298679194344 1104 90-91 0.29198412031990983 1104 92-93 0.3634902412803349 1104 94-95 0.39062640386080716 1104 96-97 0.41766252978255736 1104 98-99 0.4037509649818105 1104 100-101 0.5221231887174547 1105 1 0.2770952887325464 1105 2 0.26485478785308203 1105 3 0.1918490784595832 1105 4 0.1887745187220915 1105 5 0.16025428199202452 1105 6 0.14532110917777885 1105 7 0.19089344718273082 1105 8 0.17708685908519328 1105 9 0.24823841987088713 1105 10-11 0.18721136071588518 1105 12-13 0.2233593060183452 1105 14-15 0.29761209065544136 1105 16-17 0.315532782860771 1105 18-19 0.2981231960319306 1105 20-21 0.2816309263471837 1105 22-23 0.35207297032260243 1105 24-25 0.32370522885186404 1105 26-27 0.31378064656944105 1105 28-29 0.31382029148409174 1105 30-31 0.3166412511863257 1105 32-33 0.31503972854047646 1105 34-35 0.30361087594158676 1105 36-37 0.30788815140709147 1105 38-39 0.3314336685524566 1105 40-41 0.26415512332504676 1105 42-43 0.22475554543473208 1105 44-45 0.23706310073408332 1105 46-47 0.22521139224814846 1105 48-49 0.13924672635585722 1105 50-51 0.22293314627765426 1105 52-53 0.18918343804678983 1105 54-55 0.1453934968116073 1105 56-57 0.1266984683916732 1105 58-59 0.1412176636032001 1105 60-61 0.15231303447913547 1105 62-63 0.17158184595975712 1105 64-65 0.22900145821107998 1105 66-67 0.15210974347964878 1105 68-69 0.10723671257366618 1105 70-71 0.08426917894464481 1105 72-73 0.10002940181524878 1105 74-75 0.11559995104616405 1105 76-77 0.13123677232469078 1105 78-79 0.1074526227871857 1105 80-81 0.12236871759715484 1105 82-83 0.12055103221816665 1105 84-85 0.11466694484283124 1105 86-87 0.153220679351584 1105 88-89 0.07072535787699508 1105 90-91 0.1017132130450058 1105 92-93 0.1623250171551689 1105 94-95 0.15045924843839842 1105 96-97 0.14401188546282384 1105 98-99 0.16480928086691904 1105 100-101 0.2910848328070976 1106 1 0.2819510158395033 1106 2 0.24740554242016088 1106 3 0.22833624536541208 1106 4 0.17406398360733988 1106 5 0.16512745514048532 1106 6 0.12648831584697717 1106 7 0.12340435729520038 1106 8 0.11773049929589519 1106 9 0.12442116410534254 1106 10-11 0.12254185836158626 1106 12-13 0.15438192614978163 1106 14-15 0.1973636853580416 1106 16-17 0.20992402644894526 1106 18-19 0.2379884465379476 1106 20-21 0.2115466664653738 1106 22-23 0.23718592625446178 1106 24-25 0.2284515648896246 1106 26-27 0.2088456913920993 1106 28-29 0.2056394947984188 1106 30-31 0.14475584442368472 1106 32-33 0.06248615199388752 1106 34-35 0.11353136006637499 1106 36-37 0.1836584394005314 1106 38-39 0.19555811487516905 1106 40-41 0.17759557893753453 1106 42-43 0.1731387873027188 1106 44-45 0.02761816607538492 1106 46-47 -0.019249931210971738 1106 48-49 -0.001620606121242929 1106 50-51 0.045595204175626236 1106 52-53 -0.015589507044602158 1106 54-55 -0.06019023385714917 1106 56-57 -0.07156467284183066 1106 58-59 -0.09271665806338092 1106 60-61 -0.07234296661406603 1106 62-63 -0.045336045716190654 1106 64-65 -0.0850071930285452 1106 66-67 -0.10652961582358955 1106 68-69 -0.13322187999841617 1106 70-71 -0.10948519744698615 1106 72-73 -0.10138266761740766 1106 74-75 -0.1260188526616446 1106 76-77 -0.17463315242570587 1106 78-79 -0.1352089055850172 1106 80-81 -0.16872325241457276 1106 82-83 -0.12102859470272875 1106 84-85 -0.03964442791452427 1106 86-87 -0.011539325165948355 1106 88-89 -0.016888124919830716 1106 90-91 -0.006164686345826453 1106 92-93 0.015161934971573032 1106 94-95 0.02704092000244529 1106 96-97 0.025352820691125544 1106 98-99 0.024069672066637793 1106 100-101 0.18082140120123924 1107 1 0.20853983404565213 1107 2 0.1882411353793927 1107 3 0.16559715112661166 1107 4 0.14886528475550165 1107 5 0.111700116305812 1107 6 0.08894002728078476 1107 7 0.0032490466873014157 1107 8 0.06475235025767034 1107 9 0.06352496807985375 1107 10-11 0.10048944546602456 1107 12-13 0.08010315771022647 1107 14-15 0.07786873891830481 1107 16-17 0.11853542345500045 1107 18-19 0.10800759184731845 1107 20-21 0.1332234128315477 1107 22-23 0.10200449553573065 1107 24-25 0.11884545118947187 1107 26-27 0.06790805362946628 1107 28-29 0.10981052760087096 1107 30-31 0.11328063055009352 1107 32-33 0.17914682115279135 1107 34-35 0.18166553248568817 1107 36-37 0.18886129638767812 1107 38-39 0.16040744315448308 1107 40-41 0.1695859116601781 1107 42-43 0.22660006999080906 1107 44-45 0.1144154743357575 1107 46-47 0.10723062206948697 1107 48-49 0.029026036115539 1107 50-51 0.08450661508915402 1107 52-53 0.0932285830443007 1107 54-55 0.031256109228138484 1107 56-57 0.09291274347277323 1107 58-59 0.08363705957152234 1107 60-61 0.08872377937198195 1107 62-63 0.09217127364269828 1107 64-65 0.11696240025959526 1107 66-67 0.0812117368298999 1107 68-69 0.10523601896648671 1107 70-71 0.06885290682163259 1107 72-73 0.09573368666060134 1107 74-75 0.04077526480224947 1107 76-77 0.0299398797826953 1107 78-79 0.05920731302260407 1107 80-81 0.08462051950684213 1107 82-83 0.09366667070631962 1107 84-85 0.03859543279547495 1107 86-87 0.08727899800514294 1107 88-89 0.08854238026793126 1107 90-91 0.0905924455261875 1107 92-93 0.06871434993060532 1107 94-95 0.10827623692531674 1107 96-97 0.08856846032131926 1107 98-99 0.11108272192139168 1107 100-101 0.18250733229991667 1108 1 0.17240704943919383 1108 2 0.10020947268092328 1108 3 0.11823429844393729 1108 4 0.1004716791436806 1108 5 0.10335746755288966 1108 6 0.030734705580712784 1108 7 0.08647644256078024 1108 8 0.12050821492265129 1108 9 0.09427106198835133 1108 10-11 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28-29 -0.38692356069061873 2214 30-31 -0.4504917995929105 2214 32-33 -0.4660865214945744 2214 34-35 -0.49404279902306314 2214 36-37 -0.5193605682752178 2214 38-39 -0.5038743265678178 2214 40-41 -0.42456494808087797 2214 42-43 -0.4203524332951645 2214 44-45 -0.39291067467907226 2214 46-47 -0.4204085428229902 2214 48-49 -0.4889870777332561 2214 50-51 -0.4454274342432569 2214 52-53 -0.41709006469680077 2214 54-55 -0.472496274017999 2214 56-57 -0.47351938240710467 2214 58-59 -0.4199644484680931 2214 60-61 -0.4647648785311489 2214 62-63 -0.4490418811808965 2214 64-65 -0.41400129741933256 2214 66-67 -0.38780495562413364 2214 68-69 -0.388976667896344 2214 70-71 -0.3741821758126278 2214 72-73 -0.3842796840192264 2214 74-75 -0.33024604348607056 2214 76-77 -0.3721498917037067 2214 78-79 -0.34043195057084574 2214 80-81 -0.3714215394440572 2214 82-83 -0.30910348149474487 2214 84-85 -0.3797716752104954 2214 86-87 -0.35330587214399856 2214 88-89 -0.39763192929445523 2214 90-91 -0.40169184072330566 2214 92-93 -0.34651300288852127 2214 94-95 -0.33044504422023024 2214 96-97 -0.4004508438854941 2214 98-99 -0.42757354777970136 2214 100-101 -0.47050510645508936 2215 1 -0.5641672487517937 2215 2 -0.5153507377809596 2215 3 -0.4538382887917862 2215 4 -0.33182621095110676 2215 5 -0.33761441036210726 2215 6 -0.3280836657012003 2215 7 -0.30147972555450053 2215 8 -0.3465955209734304 2215 9 -0.38303251629976387 2215 10-11 -0.40497453463109423 2215 12-13 -0.42455628088094954 2215 14-15 -0.5119880187260932 2215 16-17 -0.4952929809134332 2215 18-19 -0.5738284030824516 2215 20-21 -0.4972843899734798 2215 22-23 -0.5813970088304146 2215 24-25 -0.5579742491990061 2215 26-27 -0.5077964574801896 2215 28-29 -0.5427510601709429 2215 30-31 -0.6004620252302502 2215 32-33 -0.5997231511150289 2215 34-35 -0.6371831097727352 2215 36-37 -0.6201100528744163 2215 38-39 -0.635776426043023 2215 40-41 -0.5748777657571296 2215 42-43 -0.5887758763433766 2215 44-45 -0.5623680516022205 2215 46-47 -0.6076481372794476 2215 48-49 -0.5511246776455465 2215 50-51 -0.5590858046655143 2215 52-53 -0.5815761730790427 2215 54-55 -0.5483624534742901 2215 56-57 -0.5659081619038346 2215 58-59 -0.5498441692974012 2215 60-61 -0.6142773656105405 2215 62-63 -0.5508782812913822 2215 64-65 -0.5836858529075002 2215 66-67 -0.6067069419104811 2215 68-69 -0.5350903927919148 2215 70-71 -0.5415954617632579 2215 72-73 -0.4640055142843593 2215 74-75 -0.43818086664094125 2215 76-77 -0.48307590819653257 2215 78-79 -0.47133439317200043 2215 80-81 -0.471041947464947 2215 82-83 -0.4927697964929081 2215 84-85 -0.4588326099653095 2215 86-87 -0.507220800400777 2215 88-89 -0.5392126187070971 2215 90-91 -0.5125673315642665 2215 92-93 -0.4898756514221958 2215 94-95 -0.4883148193801894 2215 96-97 -0.5366669868935574 2215 98-99 -0.6032066222736319 2215 100-101 -0.7147134323136903 2216 1 -0.6363225769895244 2216 2 -0.6170556440185564 2216 3 -0.5881215647922495 2216 4 -0.45375148639570995 2216 5 -0.4440974427852211 2216 6 -0.43644069519353934 2216 7 -0.42455404775419225 2216 8 -0.37151324092168636 2216 9 -0.5335639301371486 2216 10-11 -0.46503106053637566 2216 12-13 -0.4379106866083262 2216 14-15 -0.6769618216502451 2216 16-17 -0.7071055020878205 2216 18-19 -0.740016863791233 2216 20-21 -0.7368238854126616 2216 22-23 -0.8063405437856588 2216 24-25 -0.7355050496975224 2216 26-27 -0.6961900675305586 2216 28-29 -0.7211463168366947 2216 30-31 -0.6261759868380778 2216 32-33 -0.73017070928924 2216 34-35 -0.759562742864901 2216 36-37 -0.783787983021405 2216 38-39 -0.7720089632981129 2216 40-41 -0.7614374959691759 2216 42-43 -0.7115674168653356 2216 44-45 -0.6850762808246031 2216 46-47 -0.6683423124536247 2216 48-49 -0.6612209995733878 2216 50-51 -0.5689953185860048 2216 52-53 -0.5771979850862792 2216 54-55 -0.5674171745268239 2216 56-57 -0.5641514227838371 2216 58-59 -0.5746073880348703 2216 60-61 -0.5873010214212613 2216 62-63 -0.706707992859819 2216 64-65 -0.6791955293932261 2216 66-67 -0.6619649871650211 2216 68-69 -0.5720165033781939 2216 70-71 -0.571059682873603 2216 72-73 -0.5957777525519745 2216 74-75 -0.5538813780722478 2216 76-77 -0.6575871643510496 2216 78-79 -0.6050579382841903 2216 80-81 -0.6050967259968658 2216 82-83 -0.6050840789033387 2216 84-85 -0.5818739660460537 2216 86-87 -0.5192169174991363 2216 88-89 -0.5709410712064695 2216 90-91 -0.6141706852498103 2216 92-93 -0.5507578349085662 2216 94-95 -0.5640346472666167 2216 96-97 -0.5854170638145 2216 98-99 -0.6576453664910318 2216 100-101 -0.8554972212331471 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 207.0 3 81.0 4 67.0 5 140.0 6 363.0 7 831.0 8 1825.0 9 3406.0 10 5171.0 11 6160.0 12 7156.0 13 10223.0 14 16889.0 15 30548.0 16 42671.0 17 58018.0 18 56970.0 19 37179.0 20 29860.0 21 25687.0 22 24208.0 23 23900.0 24 24768.0 25 28546.0 26 35014.0 27 44442.0 28 55946.0 29 70707.0 30 92392.0 31 124474.0 32 170359.0 33 236203.0 34 330754.0 35 474613.0 36 821149.0 37 1531275.0 38 1731154.0 39 431277.0 40 342.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 24.063785943983504 37.33961326999376 17.621555476928574 20.975045309094153 2 23.335736240395207 28.983053885266806 29.622178558778266 18.05903131555972 3 22.307016408897603 28.20226503895845 24.515001145032837 24.97571740711111 4 23.550339978511687 26.67074666190836 26.85515131036944 22.923762049210513 5 23.307802990899738 27.21301143891966 25.38788681809726 24.09129875208334 6 24.746046871856006 25.938746920071832 26.1875709474979 23.127635260574262 7 23.785420597648436 27.822869486976032 24.712424876328306 23.679285039047222 8 25.364377781732937 26.006830147166465 25.992570301494748 22.636221769605843 9 23.480681399701595 26.257593992384177 26.211883871996477 24.04984073591775 10-11 24.38931051370734 26.210243774653662 25.667606938522923 23.73283877311607 12-13 23.91758510852357 26.35220331132616 25.677614569531393 24.05259701061887 14-15 23.326545655774996 26.36456920393891 26.22057466607299 24.088310474213102 16-17 23.365441782239113 26.422241542298945 26.263357112213793 23.948959563248152 18-19 22.778689364803967 26.556167639208955 27.3854494512128 23.279693544774275 20-21 21.83491868531579 26.370996509780674 28.440124204002053 23.353960600901477 22-23 21.457998230909784 26.119814440675377 29.333995774017215 23.088191554397625 24-25 21.45134197739177 25.706531882488953 30.5554015848571 22.28672455526218 26-27 21.416989380716313 25.61873807738743 31.152235146477892 21.81203739541836 28-29 21.5146584158085 25.767290928409874 31.08298284804055 21.635067807741073 30-31 21.488555620597385 25.67267986771965 30.990334112595026 21.84843039908794 32-33 21.47203739433299 25.643432643047674 31.031556024300848 21.852973938318485 34-35 21.556007207018954 25.81551820399472 30.949794072698438 21.678680516287887 36-37 21.58510093052745 25.68394716760504 30.79509033823211 21.9358615636354 38-39 21.641896894065656 25.727030095026937 30.789418334921542 21.84165467598586 40-41 21.725116648126985 25.95055410233144 30.437670606190608 21.886658643350962 42-43 21.910880451330492 25.92414549789483 30.016400973428148 22.148573077346533 44-45 21.938655803552784 26.059939483445266 29.782580799471525 22.21882391353042 46-47 22.20461732960262 26.43949293657151 29.012061549208617 22.343828184617255 48-49 22.422484656537186 26.473125430098737 28.378449075815904 22.725940837548173 50-51 22.52153576892851 26.39653149784168 28.132399895216004 22.949532838013813 52-53 22.658220543290494 26.594726551377207 27.817771787619357 22.92928111771294 54-55 22.674208686631395 26.5178202545819 27.636564015304042 23.171407043482663 56-57 22.6658041478962 26.452197258721156 27.72275453403256 23.15924405935008 58-59 22.831423385704834 26.717339242509862 27.429186243395726 23.022051128389577 60-61 22.79461030957644 26.641133884536483 27.304154008857886 23.26010179702919 62-63 22.81193363178195 26.605581286852377 27.44911779061761 23.133367290748062 64-65 22.88274250038231 26.886276913376513 27.210393853031047 23.020586733210134 66-67 22.89669909264266 26.76105062045298 27.091205138021614 23.25104514888275 68-69 22.884820879226766 26.729553946180207 27.214629284187374 23.170995890405656 70-71 22.938858978122408 26.968057650186626 27.010745856047354 23.082337515643612 72-73 22.923545255957364 26.889606676370185 26.903008559513207 23.28383950815924 74-75 22.882605125904615 26.824995969962 27.097535120177135 23.194863783956247 76-77 22.92944198328191 27.01701937930109 26.888172813093586 23.165365824323413 78-79 22.961431121090158 26.929884740218448 26.839374736850896 23.269309401840495 80-81 22.88635389298134 26.88702117833725 27.00698523543829 23.21963969324312 82-83 22.934593211757555 27.058443966521356 26.808154443738125 23.198808377982967 84-85 22.910079145174407 26.918721862003558 26.80751769053728 23.363681302284753 86-87 22.862802454819576 26.87383348496431 26.949473055462764 23.313891004753348 88-89 22.954509092281434 27.01089135924021 26.72079884294068 23.313800705537684 90-91 22.900919164253107 26.951465263281627 26.700745447061326 23.446870125403947 92-93 22.88222361565796 26.89274444356532 26.88083071988201 23.34420122089471 94-95 22.955569559190003 27.102962983605345 26.690801548445258 23.250665908759395 96-97 22.922462372223652 27.065726328824287 26.538656130071747 23.473155168880314 98-99 22.867693449704134 27.015140237002385 26.757071380522973 23.360094932770508 100-101 22.945704731106094 27.150884328441222 26.51305712023597 23.39035382021671 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1243.0 1 854.0 2 374.0 3 240.0 4 193.0 5 178.0 6 152.5 7 149.5 8 169.5 9 170.0 10 174.5 11 194.5 12 243.0 13 435.5 14 651.5 15 946.0 16 3757.0 17 4321.5 18 2424.0 19 2829.5 20 3793.0 21 5527.0 22 8075.0 23 11729.0 24 16479.5 25 22556.5 26 30239.0 27 39251.5 28 49413.5 29 60425.5 30 72623.5 31 87232.0 32 104042.0 33 120321.0 34 132789.5 35 146474.0 36 162826.0 37 182258.0 38 198550.0 39 209043.5 40 218772.5 41 230383.0 42 246416.0 43 260541.0 44 268133.0 45 268300.0 46 266775.5 47 265373.0 48 265582.0 49 262022.5 50 252237.0 51 240334.5 52 217764.5 53 190767.5 54 172860.0 55 159892.5 56 145105.0 57 129728.0 58 119408.0 59 110811.5 60 98462.0 61 86063.5 62 75876.5 63 67156.5 64 58236.5 65 49142.0 66 41349.0 67 33917.0 68 26740.0 69 21117.0 70 16097.5 71 11667.0 72 8486.5 73 6104.5 74 4139.5 75 2565.0 76 1562.0 77 1013.0 78 697.0 79 370.5 80 147.5 81 48.0 82 29.0 83 22.0 84 12.5 85 6.5 86 3.0 87 3.0 88 3.0 89 1.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29637166428118555 2 0.019483748989176118 3 1.670469515829597E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 7.744904118846312E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 4.5558259522625375E-5 16-17 0.0 18-19 0.0 20-21 3.0372173015083583E-5 22-23 0.00356113728601855 24-25 4.2521042221117014E-4 26-27 0.008891453650165719 28-29 0.011708472697314721 30-31 0.004912698985189769 32-33 3.037217301508358E-4 34-35 8.352347579147984E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 6.833738928393806E-5 50-51 0.0 52-53 0.001518608650754179 54-55 0.00271071644159621 56-57 0.005831457218896048 58-59 0.0062870398141223 60-61 0.0016249112563069715 62-63 0.00390282423243824 64-65 9.111651904525075E-5 66-67 4.2521042221117014E-4 68-69 2.8094260038952315E-4 70-71 2.7334955713575223E-4 72-73 0.0012072938773495722 74-75 7.517112821233186E-4 76-77 1.670469515829597E-4 78-79 0.0010250608392590708 80-81 0.001306003439648594 82-83 1.5186086507541792E-5 84-85 0.003644660761810029 86-87 0.0069628206637079105 88-89 0.005337909407400939 90-91 0.0020425286352643707 92-93 0.0020045634189955162 94-95 0.002680344268581126 96-97 0.00271071644159621 98-99 0.001586946040038117 100-101 0.0018147373376512438 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 6584975.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.13895939564106 #Duplication Level Percentage of deduplicated Percentage of total 1 79.69532381975564 41.55231252660705 2 12.046307195740695 12.561638434922855 3 3.1908849018457435 4.991082550004979 4 1.3661562166416952 2.849198540303358 5 0.6957816443698583 1.8138665452016205 6 0.4472009226108056 1.3989954447418806 7 0.33163566260050065 1.2103796842531815 8 0.23751879387631536 0.9907186199695075 9 0.19534349562910194 0.9166505928127499 >10 1.5278691072973618 15.354331113844784 >50 0.16843107522632367 6.278085811958553 >100 0.09623512487854326 7.680224117985783 >500 5.831400537150562E-4 0.2041179580599223 >1k 5.248076211049308E-4 0.5198955416016713 >5k 1.1662391574868567E-4 0.37649862208980395 >10k+ 8.746793681151426E-5 1.3020038956421698 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38769 0.5887493878108877 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 30185 0.458392021230149 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16695 0.2535317142434102 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 7404 0.11243778450183942 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.518608650754179E-5 0.0 0.0 0.0 0.0 2 1.518608650754179E-5 0.0 0.0 0.0 0.0 3 1.518608650754179E-5 1.518608650754179E-5 0.0 1.518608650754179E-5 0.0 4 1.518608650754179E-5 1.518608650754179E-5 0.0 1.518608650754179E-5 0.0 5 4.555825952262537E-5 1.518608650754179E-5 0.0 3.037217301508358E-5 0.0 6 4.555825952262537E-5 1.518608650754179E-5 0.0 4.555825952262537E-5 0.0 7 6.074434603016716E-5 1.518608650754179E-5 0.0 4.555825952262537E-5 0.0 8 1.2148869206033432E-4 1.518608650754179E-5 0.0 4.555825952262537E-5 0.0 9 6.985599793469223E-4 1.518608650754179E-5 0.0 4.555825952262537E-5 0.0 10-11 9.643164932289037E-4 1.518608650754179E-5 0.0 6.833738928393806E-5 0.0 12-13 0.0014426782182164703 1.518608650754179E-5 0.0 7.593043253770895E-5 0.0 14-15 0.0017084347320984513 1.518608650754179E-5 0.0 1.0630260555279253E-4 0.0 16-17 0.0020653077650256836 1.518608650754179E-5 0.0 1.2148869206033432E-4 0.0 18-19 0.0026423790523122715 1.518608650754179E-5 0.0 1.2148869206033432E-4 0.0 20-21 0.0035991025022874042 1.518608650754179E-5 0.0 1.594539083291888E-4 0.0 22-23 0.005504956358983899 1.518608650754179E-5 0.0 1.670469515829597E-4 0.0 24-25 0.006978006750215452 2.2779129761312684E-5 0.0 1.670469515829597E-4 0.0 26-27 0.007638601513293521 3.037217301508358E-5 0.0 1.670469515829597E-4 0.0 28-29 0.008716813655328988 8.352347579147984E-5 0.0 1.670469515829597E-4 0.0 30-31 0.010121526657276603 1.2908173531410522E-4 0.0 1.670469515829597E-4 0.0 32-33 0.011465495313194053 1.8223303809050147E-4 0.0 1.670469515829597E-4 0.0 34-35 0.013849710894878112 1.898260813442724E-4 0.0 1.8223303809050147E-4 0.0 36-37 0.017448813397165516 2.0501216785181418E-4 0.0 1.8223303809050147E-4 0.0 38-39 0.021837592397845093 2.1260521110558507E-4 0.0 1.9741912459804328E-4 0.0 40-41 0.027600712227457205 2.1260521110558507E-4 0.0 1.9741912459804328E-4 0.0 42-43 0.034563532891165115 2.1260521110558507E-4 0.0 1.9741912459804328E-4 0.0 44-45 0.0427336474322226 2.1260521110558507E-4 0.0 1.9741912459804328E-4 0.0 46-47 0.05272609235418509 2.2019825435935596E-4 0.0 1.9741912459804328E-4 7.593043253770895E-6 48-49 0.06392583115349716 2.2779129761312685E-4 0.0 1.9741912459804328E-4 1.518608650754179E-5 50-51 0.07823871768685531 2.2779129761312685E-4 0.0 2.0501216785181418E-4 1.518608650754179E-5 52-53 0.09448783024992502 2.2779129761312685E-4 0.0 2.1260521110558507E-4 1.518608650754179E-5 54-55 0.11169366626296987 2.2779129761312685E-4 0.0 2.2779129761312685E-4 1.518608650754179E-5 56-57 0.13129131090095253 2.4297738412066864E-4 0.0 2.5816347062821045E-4 1.518608650754179E-5 58-59 0.15410840587853408 2.4297738412066864E-4 0.0 2.809426003895231E-4 1.518608650754179E-5 60-61 0.17962862425445808 2.4297738412066864E-4 0.0 3.037217301508358E-4 1.518608650754179E-5 62-63 0.2083455138402196 2.4297738412066864E-4 0.0 3.037217301508358E-4 1.518608650754179E-5 64-65 0.24051723810644687 2.4297738412066864E-4 0.0 3.037217301508358E-4 1.518608650754179E-5 66-67 0.27561228402537596 2.4297738412066864E-4 0.0 3.037217301508358E-4 1.518608650754179E-5 68-69 0.31416216462477076 2.4297738412066864E-4 0.0 3.037217301508358E-4 1.518608650754179E-5 70-71 0.3565920903268426 2.4297738412066864E-4 0.0 3.037217301508358E-4 1.518608650754179E-5 72-73 0.4037676680625211 2.4297738412066864E-4 0.0 3.1131477340460667E-4 1.518608650754179E-5 74-75 0.45603817782148 2.4297738412066864E-4 0.0 3.4168694641969024E-4 1.518608650754179E-5 76-77 0.5112244161898869 2.4297738412066864E-4 0.0 3.6446607618100294E-4 1.518608650754179E-5 78-79 0.5695086162058323 2.4297738412066864E-4 0.0 3.796521626885448E-4 1.518608650754179E-5 80-81 0.6334951917053595 2.4297738412066864E-4 0.0 4.024312924498575E-4 1.518608650754179E-5 82-83 0.7039358539705922 2.4297738412066864E-4 0.0 4.176173789573992E-4 1.518608650754179E-5 84-85 0.7808002308285149 2.4297738412066864E-4 0.0 4.3280346546494106E-4 1.518608650754179E-5 86-87 0.8645211257445928 2.4297738412066864E-4 0.0 4.631756384800246E-4 1.518608650754179E-5 88-89 0.9544835022152703 2.4297738412066864E-4 0.0 4.707686817337955E-4 1.518608650754179E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 2610 0.0 83.05898 1 TCGACGG 3870 0.0 61.865765 2 TATCTCG 9120 0.0 60.838547 2 ATATCTC 10150 0.0 55.48392 1 ATCTCGA 19010 0.0 52.106045 1 TCTCGAG 20275 0.0 47.164337 2 CGACGGC 5410 0.0 44.42371 3 GACGGCG 6005 0.0 40.57569 4 ACGGCGC 6030 0.0 40.01363 5 CGGCGCG 6350 0.0 38.071995 6 CTCGAGG 25320 0.0 37.854633 3 TCGAGGG 25615 0.0 37.41867 4 CGAGGGC 30040 0.0 32.001633 5 AGGGCGC 31605 0.0 30.37191 7 GGGCGCG 32215 0.0 29.797033 8 GAGGGCG 32405 0.0 29.680727 6 GGCGCGC 38875 0.0 25.68171 9 CTCGACG 1435 0.0 23.830994 3 TCTCGAC 1495 0.0 23.51372 2 TATGCCG 1385 0.0 18.518702 46-47 >>END_MODULE