FastQCFastQC Report
Thu 26 May 2016
SRR2048274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19177401
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1476030.7696715524694926No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG285040.14863327934791581No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA272080.14187532502449107No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC268250.13987818265884933No Hit
ATATACATATATATGTATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTT259730.135435453427709No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA205810.10731902618086778No Hit
ATATACATATATATGTGTCGACGGCGCGCCGGATCCATATTTTTTTTTTT202180.10542617323379742No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC196360.10239135115337059No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG193880.1010981623630856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG567700.080.607981
TATCTCG754050.078.118782
ATATCTC809600.073.3803251
TCGACGG629900.072.3470842
ATCTCGA819800.071.696963
TCTCGAG764300.070.3696754
CGACGGC668000.068.256273
GACGGCG678600.066.742784
CTCGAGG801050.066.5487755
ACGGCGC680500.066.263835
TCGAGGG807050.065.8510066
CGGCGCG693100.064.994136
AGGGCGC845100.062.98689
CTCGACG74800.061.6246385
CGAGGGC889700.059.659617
GAGGGCG905300.058.635898
TCTCGAC82500.057.312434
ATATACA1854950.037.6578671
ACATATA2326650.032.611935
TATACAT2574200.032.5819052