FastQCFastQC Report
Thu 26 May 2016
SRR2048274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19177401
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT1326320.691605708197894No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT1257770.6558605099825571No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT649520.33869031575237957No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT241820.12609633599464287No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229670.11976075381643216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194910.10163525286872815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG86550.072.782171
TCGACGG119700.057.2700082
ATCTCGA676700.042.917871
CGACGGC165100.041.5129663
TCTCGAG699050.040.299672
GACGGCG177400.038.6881184
ACGGCGC179600.038.3199925
ATATACA1841200.036.6534541
CGGCGCG198500.034.6713876
CTCGAGG859800.032.8411943
ACATATA2330900.032.6051985
TCGAGGG873000.032.3717424
TATACAT2583700.032.271182
CATATAT2366850.031.1928756
CATATGT855200.030.8961734
ACATATG888450.029.9698523
ATATATG2339700.029.1731539
TATATAT2465250.028.7378858
ATATATA2546700.028.508837
CGAGGGC1006500.028.2337515
Produced by FastQC (version 0.11.5)