##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048274_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19177401 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.215166434700926 34.0 31.0 34.0 30.0 34.0 2 32.36692740585651 34.0 31.0 34.0 30.0 34.0 3 32.4410729587393 34.0 31.0 34.0 30.0 34.0 4 35.90985712818958 37.0 35.0 37.0 35.0 37.0 5 35.877688692018275 37.0 35.0 37.0 35.0 37.0 6 35.81552541973753 37.0 35.0 37.0 35.0 37.0 7 35.752677904581546 37.0 35.0 37.0 33.0 37.0 8 35.69811999029483 37.0 35.0 37.0 33.0 37.0 9 37.33669651064813 39.0 37.0 39.0 34.0 39.0 10-11 37.31175793841929 39.0 37.0 39.0 34.0 39.0 12-13 37.33471941792321 39.0 37.0 39.0 34.0 39.0 14-15 38.71471407934787 40.0 38.0 41.0 34.0 41.0 16-17 38.705751733511754 40.0 38.0 41.0 34.0 41.0 18-19 38.82480824695692 40.0 38.0 41.0 35.0 41.0 20-21 38.906603402619574 40.0 38.0 41.0 35.0 41.0 22-23 38.91968830917182 40.0 38.0 41.0 35.0 41.0 24-25 38.92785735147322 40.0 38.0 41.0 35.0 41.0 26-27 38.80867305741795 40.0 38.0 41.0 35.0 41.0 28-29 38.65273610329157 40.0 38.0 41.0 35.0 41.0 30-31 38.51657956675152 40.0 38.0 41.0 35.0 41.0 32-33 38.38740400745648 40.0 38.0 41.0 34.0 41.0 34-35 38.32082280075387 40.0 38.0 41.0 34.0 41.0 36-37 38.244359702339224 40.0 38.0 41.0 34.0 41.0 38-39 38.13303132160609 40.0 37.0 41.0 34.0 41.0 40-41 37.98893431909778 40.0 37.0 41.0 33.5 41.0 42-43 37.79487353369729 40.0 37.0 41.0 33.0 41.0 44-45 37.551680412794205 40.0 37.0 41.0 33.0 41.0 46-47 37.24814843262651 40.0 37.0 41.0 32.5 41.0 48-49 36.9551726274066 40.0 36.5 41.0 31.5 41.0 50-51 36.703306068429185 40.0 36.0 41.0 31.0 41.0 52-53 36.49644798062052 40.0 36.0 41.0 31.0 41.0 54-55 36.26212311042565 39.0 35.0 41.0 30.5 41.0 56-57 36.0100286008516 39.0 35.0 41.0 30.0 41.0 58-59 35.75430419377474 39.0 35.0 41.0 29.5 41.0 60-61 35.48609845515563 39.0 35.0 40.5 29.0 41.0 62-63 35.17801129569121 38.0 35.0 40.0 28.0 41.0 64-65 34.844488468484336 37.5 35.0 40.0 28.0 41.0 66-67 34.5126575545873 37.0 34.0 40.0 27.5 41.0 68-69 34.15291143987655 36.5 34.0 39.0 26.5 41.0 70-71 33.81554833733726 36.0 34.0 39.0 26.0 41.0 72-73 33.45521006730787 35.5 34.0 39.0 26.0 40.5 74-75 33.10237732422657 35.0 33.5 37.5 26.0 40.0 76-77 32.78290402854903 35.0 33.0 37.0 25.5 39.0 78-79 32.465342149335044 35.0 33.0 37.0 25.0 39.0 80-81 32.17223308831056 35.0 33.0 36.0 25.0 39.0 82-83 31.889563945604515 35.0 33.0 36.0 24.0 37.0 84-85 31.601402478886477 35.0 33.0 35.5 23.5 37.0 86-87 31.382464026277596 35.0 33.0 35.0 22.5 37.0 88-89 31.19077008923159 35.0 33.0 35.0 20.5 36.0 90-91 30.986414034936224 35.0 33.0 35.0 19.5 36.0 92-93 30.748282366312306 35.0 32.0 35.0 18.0 36.0 94-95 30.57470313625918 35.0 32.0 35.0 12.5 35.5 96-97 30.416371540648285 35.0 32.0 35.0 4.5 35.0 98-99 30.2414896836125 35.0 32.0 35.0 2.0 35.0 100-101 29.373607664563096 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4605983439588286 1101 2 0.3992676722248447 1101 3 0.40463517596612064 1101 4 0.2885981166333309 1101 5 0.2683214430582481 1101 6 0.26110244209105815 1101 7 0.2571068897743487 1101 8 0.2525084579083483 1101 9 0.3075870506330105 1101 10-11 0.329950495181734 1101 12-13 0.3194763750726537 1101 14-15 0.4412722474497244 1101 16-17 0.4449271093139018 1101 18-19 0.48770945508729824 1101 20-21 0.4669566548882145 1101 22-23 0.491098274400656 1101 24-25 0.4640696139411986 1101 26-27 0.4990066925067893 1101 28-29 0.4874279052934227 1101 30-31 0.45884248107795855 1101 32-33 0.5151345168392396 1101 34-35 0.5152633522586001 1101 36-37 0.5311314725783731 1101 38-39 0.5164254993456652 1101 40-41 0.5437160370141001 1101 42-43 0.4946592946635704 1101 44-45 0.45680309554269627 1101 46-47 0.45656722351233014 1101 48-49 0.4549633866932936 1101 50-51 0.5295199341393655 1101 52-53 0.5129761085116229 1101 54-55 0.5117957563629503 1101 56-57 0.46510024314436293 1101 58-59 0.49547421046522544 1101 60-61 0.47786753947715965 1101 62-63 0.5081647364863286 1101 64-65 0.48175411117828304 1101 66-67 0.48043985300419223 1101 68-69 0.4473373692839928 1101 70-71 0.44128045068148936 1101 72-73 0.4137541050293976 1101 74-75 0.42226942274603374 1101 76-77 0.42326811933286024 1101 78-79 0.4337404468737276 1101 80-81 0.4635938759183418 1101 82-83 0.4696721195407747 1101 84-85 0.4847796563586151 1101 86-87 0.47474776033749677 1101 88-89 0.4841251071118613 1101 90-91 0.465000502503667 1101 92-93 0.49864429654294185 1101 94-95 0.49090813315935833 1101 96-97 0.49218047326448655 1101 98-99 0.513115977831788 1101 100-101 0.6317389494220009 1102 1 0.4421849624159577 1102 2 0.4041461635856791 1102 3 0.36713537361221427 1102 4 0.27147386066028645 1102 5 0.2808502526519874 1102 6 0.26587357711007087 1102 7 0.23303210503355842 1102 8 0.22912052111681902 1102 9 0.29870924635018525 1102 10-11 0.2484565565765564 1102 12-13 0.2720082518571445 1102 14-15 0.399712231916304 1102 16-17 0.41858603008967776 1102 18-19 0.4388118108042107 1102 20-21 0.4412426696548408 1102 22-23 0.44955769607809515 1102 24-25 0.4490462410872951 1102 26-27 0.4357196085431383 1102 28-29 0.45200553720310666 1102 30-31 0.4278098988263963 1102 32-33 0.4502908962352947 1102 34-35 0.4825741473767806 1102 36-37 0.47917214139179265 1102 38-39 0.46707239704132064 1102 40-41 0.4935080674651573 1102 42-43 0.4840710954623759 1102 44-45 0.4279958161182691 1102 46-47 0.41560414263896917 1102 48-49 0.4183312474997791 1102 50-51 0.42059817487626106 1102 52-53 0.40888551655542216 1102 54-55 0.4019311479794965 1102 56-57 0.3838900075366354 1102 58-59 0.3655291726198513 1102 60-61 0.38580294849364805 1102 62-63 0.400746917638088 1102 64-65 0.37447672643996555 1102 66-67 0.37864504960651146 1102 68-69 0.3576567498398475 1102 70-71 0.36074561209819933 1102 72-73 0.3365926300675284 1102 74-75 0.3236195251542284 1102 76-77 0.2976118272936503 1102 78-79 0.34196923204333984 1102 80-81 0.2957077523902214 1102 82-83 0.2914385727170483 1102 84-85 0.32515088200427655 1102 86-87 0.31301686566540354 1102 88-89 0.3093702128908653 1102 90-91 0.3267564207647453 1102 92-93 0.3617594263082786 1102 94-95 0.3522845475806484 1102 96-97 0.34548186844677176 1102 98-99 0.3303460741359068 1102 100-101 0.4668593182939418 1103 1 0.4029898080465699 1103 2 0.3454027349828692 1103 3 0.33575588234973 1103 4 0.26839113099086376 1103 5 0.2711092354911173 1103 6 0.22100843345984345 1103 7 0.21004240607410907 1103 8 0.21988393529657912 1103 9 0.2485634227848692 1103 10-11 0.25859997605336105 1103 12-13 0.27121562042901104 1103 14-15 0.3724931777289484 1103 16-17 0.3715762103301117 1103 18-19 0.38847394872367147 1103 20-21 0.3757109127685254 1103 22-23 0.3875286175168924 1103 24-25 0.38793849486874876 1103 26-27 0.40728825208838515 1103 28-29 0.4293674856958276 1103 30-31 0.4041451226320518 1103 32-33 0.421885049686189 1103 34-35 0.4529133707728974 1103 36-37 0.4653224790999033 1103 38-39 0.4351544665296174 1103 40-41 0.4489029834462599 1103 42-43 0.451128263859637 1103 44-45 0.4417059123757241 1103 46-47 0.45409870229787686 1103 48-49 0.40800290372756365 1103 50-51 0.4177655912584015 1103 52-53 0.3924849681636857 1103 54-55 0.37609001173560586 1103 56-57 0.38709083037828407 1103 58-59 0.3841694156338491 1103 60-61 0.36953180123492047 1103 62-63 0.39776620488147074 1103 64-65 0.3892151696203925 1103 66-67 0.35931237907905 1103 68-69 0.36671407367198583 1103 70-71 0.374996474601204 1103 72-73 0.3348986834614749 1103 74-75 0.32325369014005645 1103 76-77 0.3477121896469484 1103 78-79 0.3580339079591255 1103 80-81 0.36049898697255855 1103 82-83 0.3754103631201673 1103 84-85 0.3667093593006534 1103 86-87 0.3589760889650151 1103 88-89 0.3681953202767083 1103 90-91 0.38553098373087025 1103 92-93 0.430450108747074 1103 94-95 0.44848274394288623 1103 96-97 0.467753475257755 1103 98-99 0.44296055677345336 1103 100-101 0.5552621321475897 1104 1 0.35903164649894137 1104 2 0.33449878663281396 1104 3 0.3113358547326115 1104 4 0.25031494375762975 1104 5 0.2521675172928681 1104 6 0.18832982532325815 1104 7 0.1716991861962356 1104 8 0.1632118004986296 1104 9 0.21287890512846985 1104 10-11 0.22552720374979884 1104 12-13 0.22239571644556833 1104 14-15 0.29384954943272845 1104 16-17 0.3621848608328193 1104 18-19 0.34454172402732297 1104 20-21 0.3446640620533188 1104 22-23 0.36347093468943115 1104 24-25 0.35565901904660535 1104 26-27 0.31049496484910577 1104 28-29 0.327691844373561 1104 30-31 0.3287907671452004 1104 32-33 0.3351455054728518 1104 34-35 0.35353501266405374 1104 36-37 0.39219766655285326 1104 38-39 0.36557678796984305 1104 40-41 0.3799348497555144 1104 42-43 0.4049394767992496 1104 44-45 0.3874865600292736 1104 46-47 0.38372966790588237 1104 48-49 0.3465088721624383 1104 50-51 0.3943913626001745 1104 52-53 0.36664886885767345 1104 54-55 0.35322493658866705 1104 56-57 0.3960237191429812 1104 58-59 0.37893476281006144 1104 60-61 0.3518447024053444 1104 62-63 0.37736474076621107 1104 64-65 0.4068073997108783 1104 66-67 0.35631029669333714 1104 68-69 0.35478358675393906 1104 70-71 0.3579921543127256 1104 72-73 0.34444686207248054 1104 74-75 0.3297279060876406 1104 76-77 0.37462389893463666 1104 78-79 0.35932063320567664 1104 80-81 0.3524631763034378 1104 82-83 0.3625129767842168 1104 84-85 0.3651999326217883 1104 86-87 0.38086805646962674 1104 88-89 0.3730028927294846 1104 90-91 0.38714045112502404 1104 92-93 0.42662131265630165 1104 94-95 0.42985611718746597 1104 96-97 0.44202562398105627 1104 98-99 0.4567528753016674 1104 100-101 0.6040940527619512 1105 1 0.2742637599150157 1105 2 0.2519778965964008 1105 3 0.1960552955577981 1105 4 0.1792230304096165 1105 5 0.16810632604881448 1105 6 0.1347519717843113 1105 7 0.16180047869019631 1105 8 0.15739432773642648 1105 9 0.20089234117406107 1105 10-11 0.20820004584459895 1105 12-13 0.22213572542383275 1105 14-15 0.28434697780686946 1105 16-17 0.3091345620292216 1105 18-19 0.2963141598102297 1105 20-21 0.2649764633766196 1105 22-23 0.3008371151449296 1105 24-25 0.2812341221341157 1105 26-27 0.28111129124555845 1105 28-29 0.3060016944527817 1105 30-31 0.2897006054536959 1105 32-33 0.302003460688411 1105 34-35 0.2886032962119742 1105 36-37 0.31202258513290104 1105 38-39 0.30889508124220555 1105 40-41 0.34288466187204136 1105 42-43 0.35272890110039157 1105 44-45 0.3580734041692182 1105 46-47 0.3420810308302009 1105 48-49 0.32810924816598686 1105 50-51 0.34253069096365607 1105 52-53 0.34997319094457424 1105 54-55 0.3157763137065501 1105 56-57 0.3532151501006524 1105 58-59 0.3514555718601784 1105 60-61 0.3626849107518524 1105 62-63 0.35310328677232405 1105 64-65 0.34638164583088127 1105 66-67 0.3010664401291834 1105 68-69 0.34325310814291043 1105 70-71 0.3239814091483595 1105 72-73 0.30021971665863845 1105 74-75 0.25804987842274585 1105 76-77 0.27730202582463903 1105 78-79 0.276475800690406 1105 80-81 0.34531248456217867 1105 82-83 0.33121399756711867 1105 84-85 0.30205341101225613 1105 86-87 0.3328103471495609 1105 88-89 0.283393865091103 1105 90-91 0.3079444406154117 1105 92-93 0.3056886725818835 1105 94-95 0.3408988737468128 1105 96-97 0.347825988219288 1105 98-99 0.37152512311253716 1105 100-101 0.4536546399144896 1106 1 0.2731543962036369 1106 2 0.24522372356803146 1106 3 0.22912604308407936 1106 4 0.1609008452676477 1106 5 0.17026611574431172 1106 6 0.13134995893032908 1106 7 0.15455856946109492 1106 8 0.10302104169706183 1106 9 0.1277816215070402 1106 10-11 0.1266674333488922 1106 12-13 0.1550292233602235 1106 14-15 0.1686720463717606 1106 16-17 0.20758819213870794 1106 18-19 0.2144890555891763 1106 20-21 0.16553282168608519 1106 22-23 0.21408648562133692 1106 24-25 0.23880323790127278 1106 26-27 0.23136044020766633 1106 28-29 0.18745440029508842 1106 30-31 0.19550956371151784 1106 32-33 0.13059233542560378 1106 34-35 0.1658909221352758 1106 36-37 0.18767778923917433 1106 38-39 0.22192131203502896 1106 40-41 0.18829493032666278 1106 42-43 0.15628661726630355 1106 44-45 0.0810200343762233 1106 46-47 0.03925273369204518 1106 48-49 0.03787861351205635 1106 50-51 0.06322359187561943 1106 52-53 0.05608850642246921 1106 54-55 0.025794338591069277 1106 56-57 0.038061696003950374 1106 58-59 0.03193979300348104 1106 60-61 0.011750474292533397 1106 62-63 0.098056379233455 1106 64-65 0.08073727201688286 1106 66-67 0.06443781157168615 1106 68-69 0.038013621774219075 1106 70-71 0.021186160067081516 1106 72-73 0.044237368598466276 1106 74-75 0.04389775277826402 1106 76-77 -0.008121079235948514 1106 78-79 0.08114290384278888 1106 80-81 0.07498098777652018 1106 82-83 0.10429338700899393 1106 84-85 0.16316796403664213 1106 86-87 0.1507732138728528 1106 88-89 0.1578194501524628 1106 90-91 0.18686802229187194 1106 92-93 0.2325243613489434 1106 94-95 0.23055721375913052 1106 96-97 0.24871401800696802 1106 98-99 0.24067638544473624 1106 100-101 0.3704760342784468 1107 1 0.21444823778158906 1107 2 0.1978856207517623 1107 3 0.1648344936694741 1107 4 0.1419730660286831 1107 5 0.12432546903238517 1107 6 0.11984921385180769 1107 7 0.06718180106529559 1107 8 0.06666843428363478 1107 9 0.08095575594325055 1107 10-11 0.1408985729018255 1107 12-13 0.05638016409162816 1107 14-15 0.08729630943768285 1107 16-17 0.13736538194093129 1107 18-19 0.13004003423299082 1107 20-21 0.14542270894682474 1107 22-23 0.1398559295562336 1107 24-25 0.15347903743310098 1107 26-27 0.12176265363999761 1107 28-29 0.1131777794674278 1107 30-31 0.11656488989101632 1107 32-33 0.1895311772248789 1107 34-35 0.1836672878167107 1107 36-37 0.1809270942102401 1107 38-39 0.15582029123006436 1107 40-41 0.13189498360996765 1107 42-43 0.19686846635592303 1107 44-45 0.16304359768079735 1107 46-47 0.16017139961401483 1107 48-49 0.16237385406134308 1107 50-51 0.1891395546114154 1107 52-53 0.1643447509188789 1107 54-55 0.11642411553426513 1107 56-57 0.16806642550837125 1107 58-59 0.16905130751197106 1107 60-61 0.19541011621386417 1107 62-63 0.23013015727693187 1107 64-65 0.2668125540464885 1107 66-67 0.2008264034077527 1107 68-69 0.21849797416295047 1107 70-71 0.15202012922972585 1107 72-73 0.1593266710821979 1107 74-75 0.1634438000502243 1107 76-77 0.19334387219004157 1107 78-79 0.21506801142326992 1107 80-81 0.21468586157801894 1107 82-83 0.2548238459131724 1107 84-85 0.17412400059715338 1107 86-87 0.22866694296060786 1107 88-89 0.20598019887867025 1107 90-91 0.1781044007695307 1107 92-93 0.19600285899545256 1107 94-95 0.22082231256428386 1107 96-97 0.24413926241711792 1107 98-99 0.21385115033450575 1107 100-101 0.2898783922218797 1108 1 0.15481866869940575 1108 2 0.10965026589499871 1108 3 0.125674508809837 1108 4 0.11203779815981108 1108 5 0.09965744031774193 1108 6 0.07267925668841002 1108 7 0.06241648082135498 1108 8 0.10461544439414894 1108 9 0.08173987486624412 1108 10-11 0.050753480586173794 1108 12-13 0.054093280446700476 1108 14-15 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94-95 -0.32327343122208774 2214 96-97 -0.38265213621805216 2214 98-99 -0.42239668005931463 2214 100-101 -0.5214475506270659 2215 1 -0.4870005984459205 2215 2 -0.4567527313911057 2215 3 -0.41791066665992105 2215 4 -0.3289069755232461 2215 5 -0.33982519109974163 2215 6 -0.34117540029261306 2215 7 -0.3181977758541237 2215 8 -0.30595202012215594 2215 9 -0.3461361700857495 2215 10-11 -0.3529711425142139 2215 12-13 -0.3796253229442925 2215 14-15 -0.49233479526386503 2215 16-17 -0.5015990550354985 2215 18-19 -0.507372013720186 2215 20-21 -0.45917959374280315 2215 22-23 -0.5133825884713161 2215 24-25 -0.4933416536069686 2215 26-27 -0.48243120620741564 2215 28-29 -0.44658603217780524 2215 30-31 -0.5069275864631848 2215 32-33 -0.5427846245985108 2215 34-35 -0.5872624054599029 2215 36-37 -0.5777635685115357 2215 38-39 -0.6008341807396747 2215 40-41 -0.5406059667411895 2215 42-43 -0.5457173140137215 2215 44-45 -0.5126822039904013 2215 46-47 -0.5380944909547978 2215 48-49 -0.5028336660014929 2215 50-51 -0.5017338175452366 2215 52-53 -0.4919211986483205 2215 54-55 -0.44344537428687403 2215 56-57 -0.45249477548809125 2215 58-59 -0.39484498287227154 2215 60-61 -0.46114223146123123 2215 62-63 -0.4095176605238251 2215 64-65 -0.43189849378180867 2215 66-67 -0.4216703680929257 2215 68-69 -0.33398252507006276 2215 70-71 -0.36891477852027776 2215 72-73 -0.3544471386648098 2215 74-75 -0.36515242650838786 2215 76-77 -0.387066133236857 2215 78-79 -0.3298470333018031 2215 80-81 -0.3522799215425394 2215 82-83 -0.3750243473240147 2215 84-85 -0.33940897986328267 2215 86-87 -0.37364011781771467 2215 88-89 -0.37426024779099976 2215 90-91 -0.3868224768005355 2215 92-93 -0.395943149222056 2215 94-95 -0.4402193452222285 2215 96-97 -0.4459779171721401 2215 98-99 -0.5006501914647359 2215 100-101 -0.5839188495485388 2216 1 -0.5830825228013516 2216 2 -0.5846080517186607 2216 3 -0.5595837940043396 2216 4 -0.437507649967948 2216 5 -0.4459978878967803 2216 6 -0.43212760162418107 2216 7 -0.416875197410711 2216 8 -0.3910410002158642 2216 9 -0.49135446292959273 2216 10-11 -0.49355972847249774 2216 12-13 -0.4650644309822738 2216 14-15 -0.6802198199762088 2216 16-17 -0.608184568075437 2216 18-19 -0.7005385450215584 2216 20-21 -0.6531219080667512 2216 22-23 -0.6896712813251753 2216 24-25 -0.6429074886728827 2216 26-27 -0.6688441546566466 2216 28-29 -0.6858466949864805 2216 30-31 -0.6363032484996936 2216 32-33 -0.6851539886829556 2216 34-35 -0.752149819780854 2216 36-37 -0.7941429153723405 2216 38-39 -0.7570440694469625 2216 40-41 -0.7625548641114719 2216 42-43 -0.6991176740393428 2216 44-45 -0.6706583145337532 2216 46-47 -0.6856133600477321 2216 48-49 -0.6399862462139936 2216 50-51 -0.5245783552786634 2216 52-53 -0.5671014953675311 2216 54-55 -0.5883776315823965 2216 56-57 -0.5948686884632934 2216 58-59 -0.6382928383021422 2216 60-61 -0.6146879592407402 2216 62-63 -0.6987890605899949 2216 64-65 -0.634498227958936 2216 66-67 -0.6504915738829098 2216 68-69 -0.5964389495057318 2216 70-71 -0.5761954491007515 2216 72-73 -0.6249283244951727 2216 74-75 -0.6083440842601391 2216 76-77 -0.6799126761206793 2216 78-79 -0.6714547521494154 2216 80-81 -0.6672790979256433 2216 82-83 -0.6402999732455719 2216 84-85 -0.5951097189071994 2216 86-87 -0.601142346048892 2216 88-89 -0.6142958593383803 2216 90-91 -0.7178767655146849 2216 92-93 -0.6374933077718161 2216 94-95 -0.6740109230112843 2216 96-97 -0.6797407378131766 2216 98-99 -0.8016102383826151 2216 100-101 -0.9565057072873842 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4494.0 3 975.0 4 1021.0 5 1290.0 6 2273.0 7 4445.0 8 8331.0 9 12938.0 10 18767.0 11 21299.0 12 23928.0 13 32438.0 14 51188.0 15 88285.0 16 121410.0 17 169419.0 18 173061.0 19 121685.0 20 102844.0 21 95715.0 22 91788.0 23 92164.0 24 95127.0 25 103704.0 26 120955.0 27 147394.0 28 180448.0 29 222740.0 30 286018.0 31 376760.0 32 501284.0 33 682642.0 34 942303.0 35 1346998.0 36 2261761.0 37 4234012.0 38 5031114.0 39 1393946.0 40 10437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.270864669125412 43.127733928007345 14.677064396752442 20.924337006114794 2 20.15293834171111 25.836972859755058 38.4398088218574 15.570279976676431 3 20.03432168523542 34.984382642467324 22.521387175933313 22.459908496363944 4 20.83128469806727 24.681399737117662 31.956358424168112 22.53095714064695 5 21.155937658079946 33.60594065900796 23.53380418962924 21.704317493282847 6 22.41293280564973 23.932726859077516 28.76140515599585 24.8929351792769 7 21.636258218723174 34.16057264485422 23.003487281722897 21.199681854699705 8 22.87717866358023 23.7298718407729 32.808395035823196 20.58455445982367 9 22.25780229552482 31.313414158675617 24.884972682168975 21.543810863630583 10-11 22.72258112556545 27.428867968083892 28.1099169798869 21.738633926463756 12-13 22.37642368744336 27.80941484197989 27.509298574921598 22.304862895655152 14-15 23.46791948197134 26.078876356222423 27.827521095214475 22.625683066591762 16-17 22.20746179318042 27.190902980023207 27.91660872085847 22.6850265059379 18-19 22.158810779416875 27.66850680131265 27.922146488984612 22.25053593028586 20-21 21.059064315287788 27.32905920510601 29.05428525243255 22.557591227173653 22-23 20.71906139955043 26.779589240149736 30.00907169564992 22.492277664649922 24-25 20.80288916327555 26.348719687952176 31.318119213443673 21.530271935328596 26-27 20.952963611181122 26.102579715319063 32.02204744842194 20.922409225077875 28-29 20.997040960326757 26.2986684842979 31.84536853446702 20.85892202090832 30-31 20.998768068699462 26.078393570762053 31.734287478657354 21.18855088188113 32-33 20.977067422670594 26.053685118918175 31.73557751835625 21.233669940054988 34-35 21.037909468941947 26.241366426095986 31.633472664978235 21.087251439983827 36-37 21.00358124648903 26.09914659447336 31.48666495527731 21.4106072037603 38-39 21.10564669320937 26.006490660543623 31.590362531398284 21.297500114848724 40-41 21.092185536507266 26.229656980109034 31.336071034860254 21.34208644852345 42-43 21.346052053664625 26.149479275111364 30.90686793272978 21.59760073849423 44-45 21.262325901200064 26.283783188347577 30.73096818489638 21.722922725555982 46-47 21.582405770208382 26.527585776612796 30.00424562223004 21.88576283094878 48-49 21.764090436031182 26.710324354290655 29.23843840282967 22.287146806848497 50-51 21.821888169309283 26.63394794737827 29.082564420486385 22.46159946282606 52-53 21.886306387019232 26.78381018002458 28.8014692334084 22.52841419954779 54-55 22.026633503089126 26.712444578066126 28.488611131701607 22.77231078714314 56-57 22.029586831743977 26.653721846548084 28.62164652219208 22.69504479951586 58-59 22.2263311999126 26.86945918677442 28.30203428291915 22.602175330393834 60-61 22.17474830238745 26.811883081881238 28.047425783666014 22.965942832065302 62-63 22.248107324623266 26.738251041000467 28.225087267052658 22.788554367323613 64-65 22.28105194765917 27.001073660739895 27.992238772971074 22.725635618629866 66-67 22.325207637295968 26.83129037225292 27.922480894219216 22.921021096231897 68-69 22.30963845435631 26.812629184016025 27.979933717096948 22.89779864453072 70-71 22.420568249701315 26.939575157018613 27.74783619671212 22.89202039656795 72-73 22.41747007013133 26.918286770661187 27.664697970982573 22.999545188224914 74-75 22.437963503558475 26.848731278555498 27.80889293268721 22.904412285198816 76-77 22.458070029181542 27.057396448547085 27.569455914492934 22.915077607778443 78-79 22.463627833856965 27.01906381164068 27.509330937988675 23.00797741651368 80-81 22.39311975866238 27.066361066350016 27.559858055835086 22.980661119152522 82-83 22.407243861282215 27.32017351781823 27.432052625078697 22.84052999582086 84-85 22.367486770467814 27.30355540605699 27.306220098313588 23.02273772516161 86-87 22.407270000949364 27.237034234731382 27.403867702707785 22.951828061611465 88-89 22.390457205039382 27.472090951603022 27.234691790318312 22.902760053039284 90-91 22.41935953603977 27.46941992482292 27.069753418270025 23.041467120867285 92-93 22.34783634381715 27.492657343824245 27.247274649652937 22.91223166270567 94-95 22.44408450256576 27.64550914653057 27.05352228778416 22.856884063119505 96-97 22.4542520538246 27.650849961264484 26.78492804173283 23.109969943178086 98-99 22.402509288371235 27.57801302341773 27.070829196774344 22.94864849143669 100-101 22.460479269359 27.707836732122775 26.78550644771577 23.04617755080246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 15944.0 1 10079.0 2 3808.0 3 2751.5 4 2047.0 5 1842.5 6 1649.5 7 1570.5 8 1553.0 9 1503.5 10 1521.0 11 1836.5 12 4898.0 13 12813.5 14 15900.5 15 14510.0 16 32467.0 17 35012.0 18 21607.5 19 23120.0 20 27286.5 21 34727.5 22 43488.0 23 57011.5 24 73265.0 25 93103.5 26 118554.0 27 147843.5 28 180100.0 29 216650.5 30 256491.0 31 296236.5 32 342460.0 33 390774.0 34 426394.5 35 468015.5 36 525665.0 37 616669.0 38 676325.5 39 678272.0 40 679494.5 41 692727.0 42 727466.5 43 756665.0 44 767947.0 45 762853.0 46 750248.0 47 738559.0 48 733151.0 49 713732.0 50 680589.5 51 647955.0 52 584050.5 53 503788.0 54 449705.5 55 409431.5 56 365941.5 57 323507.5 58 293362.0 59 269631.5 60 236490.0 61 204498.0 62 180425.0 63 159382.0 64 138224.0 65 116403.0 66 97249.5 67 79832.5 68 63106.0 69 49306.0 70 37991.5 71 28379.5 72 21275.5 73 15794.0 74 11146.0 75 7358.5 76 4572.5 77 3021.5 78 2138.0 79 1247.0 80 529.0 81 202.0 82 89.0 83 53.0 84 37.5 85 23.0 86 15.5 87 6.0 88 1.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29493569019076155 2 0.019090178069489188 3 1.0950388949993798E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 8.499587613566615E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 3.389406103569509E-5 16-17 0.0 18-19 0.0 20-21 5.2144709285684745E-6 22-23 0.003762240774962155 24-25 4.380155579997519E-4 26-27 0.009167039892423378 28-29 0.011789918769493321 30-31 0.005250972225068454 32-33 3.9369255510691986E-4 34-35 8.082429939281136E-5 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.1732559589279067E-4 50-51 0.0 52-53 0.0016973102872490388 54-55 0.002813207065962692 56-57 0.005910602797532367 58-59 0.006432049890389214 60-61 0.001679059638999049 62-63 0.004049036676033421 64-65 1.0950388949993798E-4 66-67 4.693023835711627E-4 68-69 2.9983207839268735E-4 70-71 2.972248429284031E-4 72-73 0.0012358296100707284 74-75 8.421370549638088E-4 76-77 2.007571307498863E-4 78-79 0.0010559303630351164 80-81 0.0014756952727848784 82-83 2.346511917855814E-5 84-85 0.003663165827319354 86-87 0.007261150768031601 88-89 0.005467372768604047 90-91 0.002150969258034496 92-93 0.002174434377213054 94-95 0.002813207065962692 96-97 0.002901853071748356 98-99 0.001723382641891881 100-101 0.001965855540070315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.9177401E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.9201057407714 #Duplication Level Percentage of deduplicated Percentage of total 1 71.23298619749212 29.14861327432287 2 15.258353420724962 12.487468708122528 3 5.754467942170887 7.064203100265355 4 2.576029191167008 4.216455475754711 5 1.3738794152393974 2.8109645473332656 6 0.80634974997345 1.9797550219774886 7 0.5091258059890892 1.4583437271500295 8 0.35646704281592306 1.1669335268102126 9 0.2517018377490111 0.9269699234252418 >10 1.5039689979690782 12.349653036808972 >50 0.20969493339754522 5.92863102962723 >100 0.15426034532344232 13.467175775760518 >500 0.010659579094012548 2.7808771690278227 >1k 0.0017620218897317164 1.3147922977991284 >5k 1.2761695839684013E-4 0.35272670612865237 >10k+ 1.6590204591589118E-4 2.54643667968607 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 132632 0.691605708197894 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 125777 0.6558605099825571 No Hit CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64952 0.33869031575237957 No Hit TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT 24182 0.12609633599464287 No Hit CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22967 0.11976075381643216 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19491 0.10163525286872815 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.042894185713695E-5 5.214470928568475E-6 0.0 0.0 0.0 2 1.042894185713695E-5 5.214470928568475E-6 0.0 0.0 0.0 3 2.6072354642842376E-5 5.214470928568475E-6 0.0 0.0 0.0 4 5.7359180214253226E-5 5.214470928568475E-6 0.0 0.0 0.0 5 7.300259299995864E-5 5.214470928568475E-6 0.0 0.0 0.0 6 8.864600578566408E-5 5.214470928568475E-6 0.0 0.0 0.0 7 1.0950388949993798E-4 5.214470928568475E-6 0.0 0.0 0.0 8 1.3036177321421188E-4 1.042894185713695E-5 0.0 0.0 0.0 9 0.033482117832338176 1.042894185713695E-5 0.0 0.0 0.0 10-11 0.07942421394849072 1.042894185713695E-5 0.0 0.0 0.0 12-13 0.16490503588051375 1.5643412785705424E-5 0.0 0.0 0.0 14-15 0.22668087297126444 1.5643412785705424E-5 0.0 0.0 0.0 16-17 0.3067881826113976 3.128682557141085E-5 0.0 0.0 0.0 18-19 0.40590223878616294 3.9108531964263566E-5 0.0 0.0 0.0 20-21 0.5017363927468587 4.9537473821400515E-5 0.0 0.0 0.0 22-23 0.6260467724484668 7.821706392852713E-5 0.0 0.0 0.0 24-25 0.7367213106718684 1.1211112496422221E-4 0.0 0.0 0.0 26-27 0.8633130214047253 1.4861242146420154E-4 0.0 0.0 0.0 28-29 0.9955650403305433 1.7207754064275967E-4 0.0 0.0 0.0 30-31 1.0810927925009233 2.3204395632129714E-4 0.0 0.0 0.0 32-33 1.1656402241367325 3.050465493212558E-4 0.0 0.0 0.0 34-35 1.2474422368286504 3.337261394283824E-4 0.0 0.0 0.0 36-37 1.3154989041528622 3.4415508128551935E-4 0.0 0.0 0.0 38-39 1.379751614934683 3.597984940712248E-4 0.0 0.0 0.0 40-41 1.4300165074506186 3.597984940712248E-4 0.0 0.0 0.0 42-43 1.4876676980368715 3.597984940712248E-4 0.0 0.0 0.0 44-45 1.5545745745213337 3.7022743592836176E-4 0.0 0.0 0.0 46-47 1.6177974272947622 3.754419068569302E-4 0.0 5.214470928568475E-6 0.0 48-49 1.684096817916046 3.806563777854987E-4 0.0 5.214470928568475E-6 0.0 50-51 1.7461203423759037 3.8587084871406714E-4 0.0 5.214470928568475E-6 0.0 52-53 1.8053828044790845 3.884780841783514E-4 0.0 5.214470928568475E-6 2.6072354642842377E-6 54-55 1.8638396308238012 3.9369255510691986E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 56-57 1.9149649110429512 3.962997905712041E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 58-59 1.9704755613130267 3.962997905712041E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 60-61 2.024187219112746 3.962997905712041E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 62-63 2.074944879131432 3.962997905712041E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 64-65 2.127063516062474 3.962997905712041E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 66-67 2.181687706274693 3.962997905712041E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 68-69 2.238645372227446 3.962997905712041E-4 0.0 5.214470928568475E-6 5.214470928568475E-6 70-71 2.297469818772627 3.962997905712041E-4 0.0 5.214470928568475E-6 1.042894185713695E-5 72-73 2.359084007264592 3.962997905712041E-4 0.0 5.214470928568475E-6 1.042894185713695E-5 74-75 2.4287910546376956 3.962997905712041E-4 0.0 5.214470928568475E-6 1.042894185713695E-5 76-77 2.503686500584725 3.962997905712041E-4 0.0 5.214470928568475E-6 1.042894185713695E-5 78-79 2.579218111985039 3.962997905712041E-4 0.0 5.214470928568475E-6 1.042894185713695E-5 80-81 2.6581234860761374 3.962997905712041E-4 0.0 5.214470928568475E-6 1.042894185713695E-5 82-83 2.7424414809910895 3.989070260354883E-4 0.0 5.214470928568475E-6 1.3036177321421186E-5 84-85 2.831817512706753 4.015142614997726E-4 0.0 5.214470928568475E-6 1.5643412785705424E-5 86-87 2.928423408364877 4.015142614997726E-4 0.0 5.214470928568475E-6 1.5643412785705424E-5 88-89 3.0294172813093914 4.015142614997726E-4 0.0 5.214470928568475E-6 1.5643412785705424E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 8655 0.0 72.78217 1 TCGACGG 11970 0.0 57.270008 2 ATCTCGA 67670 0.0 42.91787 1 CGACGGC 16510 0.0 41.512966 3 TCTCGAG 69905 0.0 40.29967 2 GACGGCG 17740 0.0 38.688118 4 ACGGCGC 17960 0.0 38.319992 5 ATATACA 184120 0.0 36.653454 1 CGGCGCG 19850 0.0 34.671387 6 CTCGAGG 85980 0.0 32.841194 3 ACATATA 233090 0.0 32.605198 5 TCGAGGG 87300 0.0 32.371742 4 TATACAT 258370 0.0 32.27118 2 CATATAT 236685 0.0 31.192875 6 CATATGT 85520 0.0 30.896173 4 ACATATG 88845 0.0 29.969852 3 ATATATG 233970 0.0 29.173153 9 TATATAT 246525 0.0 28.737885 8 ATATATA 254670 0.0 28.50883 7 CGAGGGC 100650 0.0 28.233751 5 >>END_MODULE