FastQCFastQC Report
Thu 26 May 2016
SRR2048273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8503863
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT816910.9606340083324484No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG164550.1935002951011793No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC163120.1918187063926124No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA149970.17635514589075577No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC117480.13814898005765144No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG115140.13539728944363286No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA110070.12943529311325924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG320400.092.857761
TCGACGG335300.088.220662
TATCTCG357700.087.7707442
CGACGGC354900.083.375283
ATATCTC382350.082.734091
GACGGCG358000.082.202034
ACGGCGC357300.082.030595
ATCTCGA385900.081.270633
CGGCGCG363450.080.493886
TCTCGAG372950.080.3339164
TCGAGGG388450.076.205946
CTCGAGG390250.076.029915
AGGGCGC403050.073.638439
CGAGGGC430400.068.783727
GAGGGCG432400.068.540838
CTCGACG21850.057.6313635
TCTCGAC24500.051.7856754
CGCGCCG767500.040.51489
GGCGCGC766800.040.292397
GCGCGCC771350.040.1522758
Produced by FastQC (version 0.11.5)