##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2048273_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8503863 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.02662378262679 31.0 31.0 34.0 28.0 34.0 2 31.13617822864738 33.0 31.0 34.0 28.0 34.0 3 31.2015549874216 33.0 31.0 34.0 28.0 34.0 4 34.70976531489277 37.0 35.0 37.0 32.0 37.0 5 34.624739015668524 37.0 35.0 37.0 32.0 37.0 6 34.58623733707846 37.0 35.0 37.0 32.0 37.0 7 34.4593983934125 37.0 35.0 37.0 31.0 37.0 8 34.3068056246908 37.0 35.0 37.0 30.0 37.0 9 35.90688843411518 39.0 37.0 39.0 30.0 39.0 10-11 35.6964146176861 39.0 37.0 39.0 30.0 39.0 12-13 35.70135407872869 39.0 37.0 39.0 30.0 39.0 14-15 37.030347678460956 40.0 38.0 41.0 30.5 41.0 16-17 36.985569734601796 40.0 37.5 41.0 30.5 41.0 18-19 36.94618463397164 40.0 37.5 41.0 30.0 41.0 20-21 37.02014149334249 40.0 38.0 41.0 30.0 41.0 22-23 37.08812259792991 40.0 38.0 41.0 31.0 41.0 24-25 37.083700901578496 40.0 38.0 41.0 31.0 41.0 26-27 37.035942782709455 40.0 38.0 41.0 30.5 41.0 28-29 36.94695716523185 40.0 37.0 41.0 30.0 41.0 30-31 36.70867475169814 40.0 36.5 41.0 30.0 41.0 32-33 36.40339143516306 40.0 35.5 41.0 30.0 41.0 34-35 36.34857781692861 40.0 35.0 41.0 30.0 41.0 36-37 36.206336990612385 40.0 35.0 41.0 30.0 41.0 38-39 36.071585819291776 40.0 35.0 41.0 29.5 41.0 40-41 35.63247791033322 40.0 35.0 41.0 27.0 41.0 42-43 35.472286359740274 40.0 35.0 41.0 26.0 41.0 44-45 35.22889556193462 39.0 35.0 41.0 24.5 41.0 46-47 34.85662986339267 39.0 35.0 41.0 23.0 41.0 48-49 34.29930585664421 39.0 34.0 41.0 16.5 41.0 50-51 33.94357546682019 39.0 34.0 41.0 10.5 41.0 52-53 33.73213026832629 39.0 33.5 41.0 7.5 41.0 54-55 33.51874018901763 39.0 33.0 40.5 6.0 41.0 56-57 33.29057500103188 38.0 33.0 40.0 2.0 41.0 58-59 33.07000371478233 38.0 33.0 40.0 2.0 41.0 60-61 32.82058600897028 37.5 33.0 40.0 2.0 41.0 62-63 32.53348201870138 37.0 32.0 40.0 2.0 41.0 64-65 32.236726062026165 36.5 32.0 40.0 2.0 41.0 66-67 31.92279073639827 36.0 32.0 39.0 2.0 41.0 68-69 31.5764512551531 35.5 31.5 39.0 2.0 41.0 70-71 31.25809676143654 35.0 31.0 39.0 2.0 40.5 72-73 30.917852980463113 35.0 31.0 37.5 2.0 40.0 74-75 30.57515537350496 35.0 31.0 37.0 2.0 39.5 76-77 30.266792103776837 35.0 31.0 37.0 2.0 39.0 78-79 29.962462059889724 35.0 30.5 36.0 2.0 39.0 80-81 29.657146228719817 35.0 30.0 36.0 2.0 37.5 82-83 29.42301786846754 35.0 30.0 35.5 2.0 37.0 84-85 29.19362588508305 34.5 30.0 35.0 2.0 37.0 86-87 28.954582111682654 34.0 30.0 35.0 2.0 36.0 88-89 28.753800067098915 34.0 30.0 35.0 2.0 36.0 90-91 28.570457155765563 34.0 29.0 35.0 2.0 36.0 92-93 28.375137275847457 34.0 29.0 35.0 2.0 35.5 94-95 28.179852380030113 34.0 29.0 35.0 2.0 35.0 96-97 27.97762005338045 34.0 29.0 35.0 2.0 35.0 98-99 27.70906792595318 34.0 29.0 35.0 2.0 35.0 100-101 26.487754388799537 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6999494916918181 1101 2 0.713561552367814 1101 3 0.7189616608736031 1101 4 0.7391766080297018 1101 5 0.7285780892233973 1101 6 0.7089343404825428 1101 7 0.7523774840135928 1101 8 0.9344848120550964 1101 9 0.9294028071190468 1101 10-11 1.075035270342319 1101 12-13 1.0081920200820491 1101 14-15 1.1094920838205553 1101 16-17 1.0475418599487085 1101 18-19 1.0751099663314676 1101 20-21 1.0649774047119323 1101 22-23 1.0093647890071864 1101 24-25 1.0024860724057234 1101 26-27 1.0740476461085464 1101 28-29 1.0935698505006002 1101 30-31 1.049248630955745 1101 32-33 1.0384185105512813 1101 34-35 1.0231210240562874 1101 36-37 0.9926986737263022 1101 38-39 1.0314198833267838 1101 40-41 1.104168184990776 1101 42-43 0.9872336788817293 1101 44-45 0.968695114091453 1101 46-47 0.817116130019734 1101 48-49 0.6706811729326176 1101 50-51 0.5610012508295625 1101 52-53 0.4946860117526626 1101 54-55 0.46575077208188276 1101 56-57 0.4456683693611083 1101 58-59 0.4672243664477662 1101 60-61 0.4547277018585305 1101 62-63 0.4683359483599716 1101 64-65 0.4606672666457996 1101 66-67 0.41583596399181033 1101 68-69 0.47657762635707357 1101 70-71 0.4668519140322509 1101 72-73 0.4883868614718523 1101 74-75 0.4679042682646113 1101 76-77 0.476548696821812 1101 78-79 0.42495839313288997 1101 80-81 0.4834277241234908 1101 82-83 0.45187703360263143 1101 84-85 0.45539422594766776 1101 86-87 0.4835522609853271 1101 88-89 0.5062517425671444 1101 90-91 0.53914858461739 1101 92-93 0.6008882417310417 1101 94-95 0.6899477072531965 1101 96-97 0.6884912772681666 1101 98-99 0.684798968464591 1101 100-101 0.7110920418507405 1102 1 0.6193762125421429 1102 2 0.6229636218829029 1102 3 0.6121808725041724 1102 4 0.6422713008565637 1102 5 0.620396534453036 1102 6 0.6722739511113147 1102 7 0.6794214780227179 1102 8 0.8193810894863418 1102 9 0.81004253500695 1102 10-11 0.9631835755335416 1102 12-13 0.8878974767587451 1102 14-15 0.9794308649420174 1102 16-17 0.9187946768636834 1102 18-19 0.9520363637209499 1102 20-21 0.9572969804484117 1102 22-23 0.9439865984247859 1102 24-25 0.9384272767795494 1102 26-27 0.9501435481219076 1102 28-29 0.9996422372561682 1102 30-31 0.9761694252145574 1102 32-33 1.0213893725640588 1102 34-35 0.9836057948853991 1102 36-37 0.9750048084550045 1102 38-39 1.006960668791372 1102 40-41 1.0457334768736288 1102 42-43 0.884260709799058 1102 44-45 0.8631918232483855 1102 46-47 0.7590110185414218 1102 48-49 0.5981393391377452 1102 50-51 0.5272066676174987 1102 52-53 0.48639118266156345 1102 54-55 0.4909327904726766 1102 56-57 0.5217480475055751 1102 58-59 0.4680767376170252 1102 60-61 0.49589485023992097 1102 62-63 0.45912719091884924 1102 64-65 0.4938045676624867 1102 66-67 0.4845744065182913 1102 68-69 0.47914085939436646 1102 70-71 0.4527738164748314 1102 72-73 0.49634952006220345 1102 74-75 0.4351371276251399 1102 76-77 0.44892179018176037 1102 78-79 0.4277129214424029 1102 80-81 0.4650056673711802 1102 82-83 0.4791115055913302 1102 84-85 0.4829587784449778 1102 86-87 0.4763165537055585 1102 88-89 0.5183864483340699 1102 90-91 0.5058590069364932 1102 92-93 0.5626978723985303 1102 94-95 0.6132876537445213 1102 96-97 0.6137816621577024 1102 98-99 0.723232382738356 1102 100-101 0.6813017046569385 1103 1 0.5275681436652064 1103 2 0.5522224225734647 1103 3 0.5394312815141227 1103 4 0.5949774868639253 1103 5 0.5446350203815413 1103 6 0.5878706614145699 1103 7 0.5967293287736695 1103 8 0.7463907815699073 1103 9 0.7266873131392231 1103 10-11 0.9154835042676623 1103 12-13 0.8383515357055416 1103 14-15 0.8968428618331998 1103 16-17 0.8500027910743739 1103 18-19 0.8606419558712943 1103 20-21 0.8955000285929344 1103 22-23 0.8745592806985698 1103 24-25 0.8539448998024142 1103 26-27 0.8619530104233206 1103 28-29 0.9220932671183562 1103 30-31 0.8869438047325104 1103 32-33 0.8965787283197528 1103 34-35 0.9025182099937652 1103 36-37 0.9086435181667127 1103 38-39 0.9113029705387632 1103 40-41 0.9185053550617539 1103 42-43 0.8841081924657317 1103 44-45 0.8215269126003122 1103 46-47 0.6882543472844347 1103 48-49 0.5914755238655829 1103 50-51 0.517747460593732 1103 52-53 0.4975397934705086 1103 54-55 0.4778267599821149 1103 56-57 0.4800921922182084 1103 58-59 0.4585704882790935 1103 60-61 0.4894646898518289 1103 62-63 0.4633087172995616 1103 64-65 0.5028951816387277 1103 66-67 0.5051419593193955 1103 68-69 0.49899715822354906 1103 70-71 0.4736229777054355 1103 72-73 0.4960460420822983 1103 74-75 0.5451657592709118 1103 76-77 0.46739810519289193 1103 78-79 0.488479631430522 1103 80-81 0.4948568633052659 1103 82-83 0.4359208710744191 1103 84-85 0.4095800771849305 1103 86-87 0.4350855030445153 1103 88-89 0.4952247719874663 1103 90-91 0.49378346510509274 1103 92-93 0.534604460562619 1103 94-95 0.5226276033762147 1103 96-97 0.5138971607208056 1103 98-99 0.534977793075285 1103 100-101 0.5734180924298826 1104 1 0.5652995160050338 1104 2 0.5999891094879004 1104 3 0.6338232877754457 1104 4 0.6625429924099819 1104 5 0.6530673627214014 1104 6 0.609434161972672 1104 7 0.6472822634955548 1104 8 0.7972082350315901 1104 9 0.8060039595581259 1104 10-11 0.9119574636585597 1104 12-13 0.7760203937772872 1104 14-15 0.8966830808417825 1104 16-17 0.8574325928355293 1104 18-19 0.9260984347843646 1104 20-21 0.9141999899328894 1104 22-23 0.8838525367863781 1104 24-25 0.8571098289180128 1104 26-27 0.9189292234539366 1104 28-29 0.935798550667414 1104 30-31 0.8732350975611283 1104 32-33 0.8944526086697877 1104 34-35 0.8910971314071858 1104 36-37 0.8658526844978311 1104 38-39 0.8414890377797164 1104 40-41 0.897967790432368 1104 42-43 0.8176830542876132 1104 44-45 0.7752352548243024 1104 46-47 0.6552098672964561 1104 48-49 0.5365166668435108 1104 50-51 0.4428960601422034 1104 52-53 0.41702581849514786 1104 54-55 0.3881858345099616 1104 56-57 0.3907191840135624 1104 58-59 0.3418251571678539 1104 60-61 0.35862966514152106 1104 62-63 0.371111693893603 1104 64-65 0.32706167120911545 1104 66-67 0.30736079639770253 1104 68-69 0.3270283653117616 1104 70-71 0.3456133484002031 1104 72-73 0.31666323550641806 1104 74-75 0.4027512336766996 1104 76-77 0.3554376788253606 1104 78-79 0.3366676676665712 1104 80-81 0.3870412175161704 1104 82-83 0.32275822628566786 1104 84-85 0.3325082909589234 1104 86-87 0.37087504340870225 1104 88-89 0.40875919348061984 1104 90-91 0.383274923472122 1104 92-93 0.4474285180662818 1104 94-95 0.5303109647007958 1104 96-97 0.5147148185243431 1104 98-99 0.5080672146487402 1104 100-101 0.5803904337200727 1105 1 0.4184638641402181 1105 2 0.38178338965390424 1105 3 0.4115014098317431 1105 4 0.4523433187097865 1105 5 0.4148488409010227 1105 6 0.4407194932320806 1105 7 0.44751206657021925 1105 8 0.6120462908073137 1105 9 0.5786501867036122 1105 10-11 0.708104614528061 1105 12-13 0.6632963809171457 1105 14-15 0.6558425226530602 1105 16-17 0.6439731412835528 1105 18-19 0.6687385211541041 1105 20-21 0.6577903730189618 1105 22-23 0.6333966258342372 1105 24-25 0.645841535535979 1105 26-27 0.6887623816389805 1105 28-29 0.7377641553421199 1105 30-31 0.6891569979976921 1105 32-33 0.6963855253248568 1105 34-35 0.6776792995887462 1105 36-37 0.6654653369406276 1105 38-39 0.6610811471436406 1105 40-41 0.7514513840306023 1105 42-43 0.6139082635456958 1105 44-45 0.5625897185349089 1105 46-47 0.5160935775038524 1105 48-49 0.46505291169746954 1105 50-51 0.3931614059628288 1105 52-53 0.34495619542251177 1105 54-55 0.27621348526196243 1105 56-57 0.24748420032581464 1105 58-59 0.2306396063621321 1105 60-61 0.26995822055774 1105 62-63 0.20853504952195578 1105 64-65 0.21968920253212332 1105 66-67 0.25460987505547905 1105 68-69 0.33332362206657606 1105 70-71 0.26149325883064733 1105 72-73 0.27595168617203925 1105 74-75 0.29810253393165453 1105 76-77 0.24594360299506945 1105 78-79 0.27118352045265226 1105 80-81 0.3266360363719798 1105 82-83 0.27792858192584546 1105 84-85 0.28388827360963376 1105 86-87 0.31961720006349026 1105 88-89 0.28280574499857636 1105 90-91 0.3067081721816294 1105 92-93 0.28192062207529034 1105 94-95 0.30762208571260885 1105 96-97 0.359027485701386 1105 98-99 0.4563206730904952 1105 100-101 0.42744772418329546 1106 1 0.3784352429371616 1106 2 0.392504584097189 1106 3 0.3426141855344973 1106 4 0.4473963341539289 1106 5 0.43189545730916024 1106 6 0.4012768222108818 1106 7 0.48740302924972667 1106 8 0.5939282774338608 1106 9 0.5121970795781934 1106 10-11 0.6541665351302655 1106 12-13 0.6035506408537259 1106 14-15 0.6164745198376522 1106 16-17 0.5946197987495196 1106 18-19 0.5979683425025115 1106 20-21 0.5920837410596604 1106 22-23 0.539318318646167 1106 24-25 0.5184271141271779 1106 26-27 0.5865634169356468 1106 28-29 0.6283771385868135 1106 30-31 0.582925362346856 1106 32-33 0.6202375084752276 1106 34-35 0.5923826590990657 1106 36-37 0.6007761623204857 1106 38-39 0.662115214932065 1106 40-41 0.7331532796276718 1106 42-43 0.6406163651717947 1106 44-45 0.5728043139796952 1106 46-47 0.6044179797486962 1106 48-49 0.4632421461323091 1106 50-51 0.45147644054480907 1106 52-53 0.42276535900212053 1106 54-55 0.3990068323967435 1106 56-57 0.4061439145375516 1106 58-59 0.3743996556346758 1106 60-61 0.42511024662729824 1106 62-63 0.374959222676587 1106 64-65 0.3951008142594503 1106 66-67 0.33034312655690456 1106 68-69 0.32249301474507064 1106 70-71 0.3390942105272927 1106 72-73 0.3858724178767119 1106 74-75 0.4207824715656905 1106 76-77 0.3973242229474785 1106 78-79 0.4104861599351395 1106 80-81 0.4481320969106761 1106 82-83 0.4152882947280929 1106 84-85 0.41824010924949206 1106 86-87 0.426020690483071 1106 88-89 0.4172251389939561 1106 90-91 0.4075485512287962 1106 92-93 0.40451150760323173 1106 94-95 0.43620903144428524 1106 96-97 0.4403546852930056 1106 98-99 0.4567406172996158 1106 100-101 0.4901382722754448 1107 1 0.25557200122008084 1107 2 0.24009736747015253 1107 3 0.27982637062958915 1107 4 0.3423753825315501 1107 5 0.3034274402345005 1107 6 0.3043863999540477 1107 7 0.23702901321430403 1107 8 0.4046752814128425 1107 9 0.32552461714273306 1107 10-11 0.5187999155523002 1107 12-13 0.3804255144036617 1107 14-15 0.4132591235470997 1107 16-17 0.3817046139758986 1107 18-19 0.4741092694281974 1107 20-21 0.5139822281231474 1107 22-23 0.418143380953623 1107 24-25 0.40801388332894817 1107 26-27 0.45223595379974313 1107 28-29 0.4840130341137652 1107 30-31 0.4737839062389497 1107 32-33 0.4721330902395877 1107 34-35 0.4225127933868933 1107 36-37 0.3810239940783262 1107 38-39 0.42305209502635677 1107 40-41 0.43291178414750675 1107 42-43 0.3087869773359415 1107 44-45 0.23009355431697287 1107 46-47 0.24771388885074686 1107 48-49 0.13250425256597254 1107 50-51 0.17753439015327643 1107 52-53 0.20713875772219836 1107 54-55 0.2104917434931366 1107 56-57 0.13529795217849738 1107 58-59 0.14772712646656316 1107 60-61 0.07289859884508587 1107 62-63 0.1112549959355178 1107 64-65 0.13593603112816055 1107 66-67 0.1485502460815411 1107 68-69 0.16877733964756558 1107 70-71 0.22675413844938674 1107 72-73 0.2578830733163393 1107 74-75 0.2909348592738823 1107 76-77 0.3047463852586141 1107 78-79 0.25165110000321533 1107 80-81 0.27728999277251276 1107 82-83 0.29631791223675563 1107 84-85 0.3151508440549513 1107 86-87 0.29578944217168157 1107 88-89 0.32615721694556044 1107 90-91 0.3121290213544192 1107 92-93 0.3246263984668474 1107 94-95 0.33970950014525414 1107 96-97 0.2870946522759432 1107 98-99 0.2974986224495879 1107 100-101 0.4156684663567418 1108 1 0.2445266186460593 1108 2 0.2732592251913921 1108 3 0.27732452986678524 1108 4 0.28679708314435715 1108 5 0.27729204842855637 1108 6 0.29077858333085516 1108 7 0.26760522474874193 1108 8 0.43437253699935496 1108 9 0.40208511461163 1108 10-11 0.495967322716119 1108 12-13 0.49754517807423326 1108 14-15 0.515820304946601 1108 16-17 0.421553020804474 1108 18-19 0.4042049779371979 1108 20-21 0.43783246152693067 1108 22-23 0.3237082080037368 1108 24-25 0.37108919244117544 1108 26-27 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-0.4490292541745262 2215 72-73 -0.5427647660445452 2215 74-75 -0.5504637479816878 2215 76-77 -0.5008748276922041 2215 78-79 -0.5045406938005641 2215 80-81 -0.5088209362506255 2215 82-83 -0.6027598973892374 2215 84-85 -0.6070346120255046 2215 86-87 -0.6657403581989705 2215 88-89 -0.7737819517661713 2215 90-91 -0.8289363221600148 2215 92-93 -0.6968794466881079 2215 94-95 -0.6841853093435759 2215 96-97 -0.817408414452121 2215 98-99 -0.9291980276076934 2215 100-101 -1.0453502419741696 2216 1 -1.117544606031931 2216 2 -1.0622381770883784 2216 3 -1.1794062912129846 2216 4 -1.117964659995593 2216 5 -1.1433967102740183 2216 6 -1.1315850342632672 2216 7 -1.1083965240203568 2216 8 -1.1325970219371868 2216 9 -1.3534996559889834 2216 10-11 -1.2913272754972454 2216 12-13 -1.334151549344753 2216 14-15 -1.6944136596752202 2216 16-17 -1.493476442436389 2216 18-19 -1.3894178983785253 2216 20-21 -1.5013530184980581 2216 22-23 -1.408293331557985 2216 24-25 -1.4721254082155255 2216 26-27 -1.7470779652396615 2216 28-29 -1.6847206739057725 2216 30-31 -1.5617395711245976 2216 32-33 -1.5299624702265149 2216 34-35 -1.5271175629445892 2216 36-37 -1.5686900516159028 2216 38-39 -1.7252616132612957 2216 40-41 -1.6190547350728437 2216 42-43 -1.5480963858440617 2216 44-45 -1.4279735358630248 2216 46-47 -1.2549333890942904 2216 48-49 -1.2456291513948727 2216 50-51 -1.2550364120165511 2216 52-53 -1.19984823222795 2216 54-55 -1.2135283492660065 2216 56-57 -1.2318884076500751 2216 58-59 -1.1017876840034049 2216 60-61 -1.1702898432084083 2216 62-63 -1.149457761933462 2216 64-65 -1.107840938538157 2216 66-67 -1.1043741567317227 2216 68-69 -1.1916448209440738 2216 70-71 -1.1342363965813647 2216 72-73 -1.093355013810541 2216 74-75 -1.138511127133917 2216 76-77 -1.1569249055000697 2216 78-79 -1.1234957184894228 2216 80-81 -1.1290334258386636 2216 82-83 -1.1465980509168325 2216 84-85 -1.1887347377822088 2216 86-87 -1.1060989546567868 2216 88-89 -1.1493365637800323 2216 90-91 -1.2229731522894802 2216 92-93 -1.1799419688999286 2216 94-95 -1.2074977534549518 2216 96-97 -1.283063699825565 2216 98-99 -1.3164054271865773 2216 100-101 -1.4653526551482443 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 172516.0 3 34119.0 4 19652.0 5 13848.0 6 12649.0 7 13859.0 8 17040.0 9 19264.0 10 21103.0 11 22750.0 12 25887.0 13 33039.0 14 45392.0 15 72203.0 16 79692.0 17 113700.0 18 175979.0 19 83326.0 20 50848.0 21 44204.0 22 46916.0 23 52146.0 24 59051.0 25 66746.0 26 77409.0 27 92731.0 28 110966.0 29 128442.0 30 146505.0 31 176059.0 32 220312.0 33 283556.0 34 384747.0 35 577986.0 36 1002241.0 37 1722247.0 38 1850291.0 39 434229.0 40 213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.789626420275923 38.27814993323787 16.374137065221266 19.558086581264938 2 22.386807763071182 27.758508324274487 33.283002271192615 16.57168164146172 3 21.423890782734404 30.338758087013158 22.89763540158832 25.33971572866412 4 25.129923652535197 25.797653625960375 27.582067494288797 21.490355227215634 5 21.83767284395917 29.324962704097917 24.22046887587992 24.61689557606299 6 21.957356892274674 24.99304908358459 28.179156283416734 24.870437740723997 7 25.351118838485338 25.792886926938678 23.7274294187158 25.128564815860184 8 28.905460723638264 24.969979933305467 25.129024541301614 20.995534801754655 9 20.93844288121449 28.592380367278547 25.265476776250612 25.20369997525635 10-11 27.03002800010232 25.34545269576329 24.787761694225242 22.83675760990915 12-13 25.084385595243642 25.509977141206562 24.56875726539393 24.836879998155865 14-15 24.88160564578752 25.432706203291982 24.87253197469369 24.813156176226805 16-17 24.904645535435165 27.258557085539454 24.753548550388675 23.08324882863671 18-19 22.940790449814934 25.335652396052804 26.82598108473304 24.89757606939922 20-21 22.748073691499542 28.96551095507559 25.442603284096872 22.843812069327992 22-23 20.909354745090454 27.147117813573164 29.22682499464108 22.716702446695304 24-25 20.829102128293194 25.19632909001393 31.284636191640246 22.689932590052628 26-27 20.80886996712867 25.106823006403733 33.44221643331354 20.642090593154062 28-29 20.860927658587542 25.139332965918644 33.45916463259589 20.540574742897924 30-31 20.94854457476816 24.969684888662307 33.357018575980646 20.724751960588886 32-33 20.93323398276388 24.898649540670235 33.47149382158991 20.69662265497598 34-35 21.02840202503324 25.023362963663388 33.39326083464055 20.554974176662817 36-37 20.989582399085833 24.904056151007133 33.269730498572784 20.836630951334246 38-39 21.11665559253859 24.96755252045175 33.108433545026905 20.80735834198276 40-41 21.64359167897069 25.543935211426827 31.610450063572355 21.202023046030128 42-43 21.946214124280228 25.587641148926497 31.086696744557674 21.3794479822356 44-45 22.098217082190676 25.894648305676665 30.578106504525305 21.429028107607362 46-47 22.346596656812217 26.378552808270793 29.713929651083355 21.56092088383363 48-49 22.603961887013984 26.81959625834854 28.446051113001307 22.130390741636166 50-51 22.891329785625913 26.989491807793264 27.64839629108235 22.470782115498476 52-53 23.040722433945398 27.125053371945242 27.208936806146333 22.62528738796303 54-55 23.1792045097032 26.816218676178284 27.12953042828391 22.875046385834608 56-57 23.12764699228264 26.6846940234718 27.45740091350922 22.730258070736344 58-59 23.157670767222022 26.917631509564465 27.29796065598689 22.62673706722662 60-61 23.177559240906017 26.878419475416603 27.183749371592437 22.760271912084942 62-63 23.222563939777054 26.89353564366926 27.262462358303708 22.621438058249982 64-65 23.323459777323496 27.109290929338826 27.027228625402287 22.540020667935387 66-67 23.321988561194598 26.994838444343173 26.93038958240055 22.75278341206168 68-69 23.298571398827914 26.997643759102335 27.042419511570827 22.661365330498928 70-71 23.350532546327457 27.175321114217297 26.895534703212864 22.57861163624238 72-73 23.34899984402888 27.137110139839987 26.775130111200586 22.738759904930543 74-75 23.360571227660245 27.10510042677158 26.928544432601768 22.605783912966405 76-77 23.415915794049997 27.313301672822092 26.706869258179967 22.563913274947943 78-79 23.390755116783556 27.24536598902445 26.666085943636304 22.69779295055569 80-81 23.343144698789434 27.190316315810804 26.827076404251315 22.63946258114845 82-83 23.337255234844367 27.305481891221266 26.723179226193004 22.63408364774136 84-85 23.328169234970282 27.140911530030028 26.772204789599268 22.758714445400425 86-87 23.28118741558963 27.024664960071572 26.97721073763109 22.716936886707707 88-89 23.32961589124723 27.122124502269056 26.79632574440219 22.751933862081525 90-91 23.38125431654437 27.025119236771722 26.69934862937345 22.89427781731046 92-93 23.3744837474255 27.015049612478244 26.859644470962575 22.750822169133684 94-95 23.417570791914375 27.215205619911142 26.709340919588353 22.65788266858613 96-97 23.387172477396618 27.235046947749307 26.516873428115005 22.860907146739066 98-99 23.38905286519532 27.175354849288375 26.707376416606493 22.728215868909814 100-101 23.593205553908128 27.251642156600393 26.29993061202453 22.855221677466954 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 472.0 1 411.5 2 337.5 3 360.0 4 384.5 5 398.0 6 427.5 7 449.0 8 469.0 9 464.0 10 465.5 11 483.5 12 502.5 13 540.0 14 603.5 15 684.5 16 1248.0 17 1571.5 18 1628.5 19 2403.0 20 3769.0 21 5917.0 22 9232.5 23 14088.5 24 20572.0 25 28984.5 26 39353.5 27 52298.0 28 67019.0 29 82435.5 30 99677.0 31 120012.5 32 143517.5 33 166744.5 34 184338.0 35 202845.5 36 224189.5 37 248401.5 38 267947.0 39 278379.0 40 287016.5 41 299398.0 42 319216.0 43 335917.0 44 344098.5 45 345391.5 46 343463.0 47 340597.0 48 339041.5 49 331665.5 50 317222.5 51 302082.0 52 274985.0 53 240804.5 54 216945.0 55 198875.0 56 180233.0 57 161767.5 58 147395.0 59 135825.5 60 120914.0 61 105841.5 62 93928.5 63 83146.0 64 72469.0 65 61579.5 66 51125.0 67 42292.5 68 34420.5 69 27285.5 70 20884.5 71 15706.0 72 11748.0 73 8539.0 74 6003.0 75 4037.5 76 2670.5 77 1853.0 78 1291.0 79 809.0 80 478.0 81 284.0 82 171.0 83 123.5 84 106.0 85 77.5 86 54.5 87 45.5 88 36.0 89 32.0 90 26.0 91 22.5 92 19.5 93 16.0 94 16.0 95 15.0 96 11.0 97 7.0 98 4.0 99 7.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03276158141305898 2 0.05649197311856976 3 0.04486196449778177 4 0.061360348820294966 5 0.05809124629594809 6 0.01631023453693927 7 0.008925355453162875 8 0.01431114306521636 9 0.00789053163250631 10-11 0.014293504022818806 12-13 0.014428736681200061 14-15 0.014587488062778056 16-17 0.01619264092095557 18-19 0.01855039292142877 20-21 0.017938906118313523 22-23 0.019761607166060884 24-25 0.018309326008662183 26-27 0.011912233299148868 28-29 0.014217068172429402 30-31 0.0079728471636949 32-33 0.015422402736262332 34-35 0.012805944780624993 36-37 0.02522971030930296 38-39 0.03313200130340764 40-41 0.034331456186441386 42-43 0.038417834341874985 44-45 0.035871932555827865 46-47 0.034860627458368036 48-49 0.04554400747048724 50-51 0.037183101374046124 52-53 0.039705484436896504 54-55 0.05292888655426363 56-57 0.04707860415907453 58-59 0.04844856978528464 60-61 0.04943635615954772 62-63 0.0526643009183003 64-65 0.04966566371071594 66-67 0.03909987731458044 68-69 0.03069193377174585 70-71 0.023759790109506705 72-73 0.026917178698669064 74-75 0.030503783986171932 76-77 0.02846941442965391 78-79 0.034878266500765594 80-81 0.03536628000709795 82-83 0.048913064568420254 84-85 0.04884838807962922 86-87 0.048125187341329465 88-89 0.05045942061860592 90-91 0.04543229353530272 92-93 0.03738301052121842 94-95 0.03380228491451473 96-97 0.034372613952035676 98-99 0.026999494229857653 100-101 0.028104874220104437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 8503863.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.866998756623815 #Duplication Level Percentage of deduplicated Percentage of total 1 79.08487669518598 41.0189520121696 2 12.40107091103045 12.864126590464403 3 3.5521067362062517 5.527113470106143 4 1.4262720418828183 2.9590580089177383 5 0.7519062169045724 1.9499559408643587 6 0.46132041059819323 1.4356383097741006 7 0.3180992633937282 1.1549197869247816 8 0.23213954127506406 0.9632305038941577 9 0.18220005654897975 0.8505153095834451 >10 1.3370827143384594 13.524517809313608 >50 0.13968473585304486 5.191895560196656 >100 0.11153664414035297 9.394243287208589 >500 6.909547466325538E-4 0.2291956252738486 >1k 8.288819273895349E-4 0.921322274084925 >5k 2.302449601198854E-5 0.06010466509827347 >10k+ 1.611714720839198E-4 1.9552108461255169 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT 81691 0.9606340083324484 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG 16455 0.1935002951011793 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC 16312 0.1918187063926124 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA 14997 0.17635514589075577 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC 11748 0.13814898005765144 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG 11514 0.13539728944363286 No Hit ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA 11007 0.12943529311325924 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3518723196740118E-5 0.0 1.1759361598370059E-5 0.0 0.0 2 3.527808479511017E-5 0.0 1.1759361598370059E-5 0.0 0.0 3 3.527808479511017E-5 0.0 1.1759361598370059E-5 0.0 0.0 4 4.7037446393480235E-5 0.0 1.1759361598370059E-5 0.0 0.0 5 4.7037446393480235E-5 0.0 1.1759361598370059E-5 0.0 0.0 6 9.407489278696047E-5 0.0 1.1759361598370059E-5 0.0 0.0 7 1.2935297758207064E-4 0.0 2.3518723196740118E-5 0.0 0.0 8 1.2935297758207064E-4 0.0 2.3518723196740118E-5 1.1759361598370059E-5 0.0 9 2.1166850877066105E-4 0.0 2.3518723196740118E-5 1.1759361598370059E-5 0.0 10-11 3.175027631559916E-4 0.0 2.3518723196740118E-5 1.1759361598370059E-5 0.0 12-13 5.526899951233927E-4 0.0 2.3518723196740118E-5 1.1759361598370059E-5 0.0 14-15 6.526445687095382E-4 0.0 2.3518723196740118E-5 1.1759361598370059E-5 0.0 16-17 8.290349926850892E-4 0.0 2.3518723196740118E-5 1.1759361598370059E-5 0.0 18-19 9.87786374263085E-4 0.0 2.9398403995925145E-5 1.1759361598370059E-5 0.0 20-21 0.0011759361598370059 0.0 4.115776559429521E-5 1.1759361598370059E-5 0.0 22-23 0.0020931663645098707 1.1759361598370059E-5 5.879680799185029E-5 1.1759361598370059E-5 0.0 24-25 0.0030868324195721403 4.115776559429521E-5 7.055616959022035E-5 1.1759361598370059E-5 0.0 26-27 0.003545447521908573 4.7037446393480235E-5 8.819521198777544E-5 1.1759361598370059E-5 0.0 28-29 0.004262768579409146 4.7037446393480235E-5 9.407489278696047E-5 1.1759361598370059E-5 0.0 30-31 0.0056915310136111084 5.291712719266526E-5 1.2935297758207064E-4 1.1759361598370059E-5 0.0 32-33 0.007496593018960912 5.879680799185029E-5 1.587513815779958E-4 1.1759361598370059E-5 5.879680799185029E-6 34-35 0.010036615124208845 5.879680799185029E-5 1.8814978557392094E-4 1.1759361598370059E-5 1.1759361598370059E-5 36-37 0.013570303284519048 7.055616959022035E-5 1.8814978557392094E-4 1.1759361598370059E-5 1.1759361598370059E-5 38-39 0.01815645430788337 7.055616959022035E-5 1.8814978557392094E-4 1.1759361598370059E-5 1.1759361598370059E-5 40-41 0.02350108415434256 7.055616959022035E-5 1.8814978557392094E-4 1.1759361598370059E-5 1.1759361598370059E-5 42-43 0.030286235796602085 7.055616959022035E-5 1.9402946637310595E-4 1.1759361598370059E-5 1.1759361598370059E-5 44-45 0.03863538253144483 7.055616959022035E-5 1.99909147172291E-4 1.1759361598370059E-5 1.1759361598370059E-5 46-47 0.04808990925653435 7.055616959022035E-5 1.99909147172291E-4 1.1759361598370059E-5 1.1759361598370059E-5 48-49 0.05926718245578509 7.055616959022035E-5 1.99909147172291E-4 1.1759361598370059E-5 1.1759361598370059E-5 50-51 0.07166154958046714 7.055616959022035E-5 2.1166850877066105E-4 1.1759361598370059E-5 1.1759361598370059E-5 52-53 0.08556111498974053 7.055616959022035E-5 2.1166850877066105E-4 1.1759361598370059E-5 1.1759361598370059E-5 54-55 0.10121870495797028 7.055616959022035E-5 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 56-57 0.11981613532579252 7.643585038940537E-5 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 58-59 0.1393837130254803 8.819521198777544E-5 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 60-61 0.1616030267656005 9.407489278696047E-5 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 62-63 0.18699736813728068 1.0583425438533053E-4 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 64-65 0.21442607906547884 1.0583425438533053E-4 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 66-67 0.2444477292261176 1.0583425438533053E-4 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 68-69 0.2766860190480491 1.1759361598370058E-4 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 70-71 0.3123521627759055 1.1759361598370058E-4 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 72-73 0.35084643296816986 1.1759361598370058E-4 2.1166850877066105E-4 2.3518723196740118E-5 1.1759361598370059E-5 74-75 0.393779862163819 1.1759361598370058E-4 2.1166850877066105E-4 2.9398403995925145E-5 1.1759361598370059E-5 76-77 0.44162870450758673 1.1759361598370058E-4 2.1166850877066105E-4 3.527808479511017E-5 1.1759361598370059E-5 78-79 0.49287012267248426 1.2347329678288562E-4 2.1166850877066105E-4 4.7037446393480235E-5 1.1759361598370059E-5 80-81 0.5485330607983689 1.2935297758207064E-4 2.1166850877066105E-4 4.7037446393480235E-5 1.1759361598370059E-5 82-83 0.6092936821771471 1.2935297758207064E-4 2.1166850877066105E-4 4.7037446393480235E-5 1.1759361598370059E-5 84-85 0.6739701709681823 1.2935297758207064E-4 2.1166850877066105E-4 5.879680799185029E-5 1.1759361598370059E-5 86-87 0.7437031852465168 1.2935297758207064E-4 2.1166850877066105E-4 5.879680799185029E-5 1.1759361598370059E-5 88-89 0.8175108183186864 1.2935297758207064E-4 2.1166850877066105E-4 7.055616959022035E-5 1.1759361598370059E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGACG 32040 0.0 92.85776 1 TCGACGG 33530 0.0 88.22066 2 TATCTCG 35770 0.0 87.770744 2 CGACGGC 35490 0.0 83.37528 3 ATATCTC 38235 0.0 82.73409 1 GACGGCG 35800 0.0 82.20203 4 ACGGCGC 35730 0.0 82.03059 5 ATCTCGA 38590 0.0 81.27063 3 CGGCGCG 36345 0.0 80.49388 6 TCTCGAG 37295 0.0 80.333916 4 TCGAGGG 38845 0.0 76.20594 6 CTCGAGG 39025 0.0 76.02991 5 AGGGCGC 40305 0.0 73.63843 9 CGAGGGC 43040 0.0 68.78372 7 GAGGGCG 43240 0.0 68.54083 8 CTCGACG 2185 0.0 57.631363 5 TCTCGAC 2450 0.0 51.785675 4 CGCGCCG 76750 0.0 40.5148 9 GGCGCGC 76680 0.0 40.29239 7 GCGCGCC 77135 0.0 40.152275 8 >>END_MODULE