FastQCFastQC Report
Thu 26 May 2016
SRR2048273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8503863
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT554890.6525152157319563No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT426690.501760200040852No Hit
CTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246910.2903503972253551No Hit
TATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTT106390.12510784804505903No Hit
CGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89230.10492878354225602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG35000.084.2663351
TCGACGG47600.066.266392
TATCTCG141000.057.5104262
ATATCTC142400.057.282941
ATCTCGA263250.049.773231
TCTCGAG273050.046.677832
CGACGGC69500.045.6512153
GACGGCG76050.041.656694
ACGGCGC77950.040.580395
CGGCGCG85150.037.260616
CTCGAGG345000.037.0747263
TCGAGGG348650.036.6863754
CGAGGGC411200.031.2213065
AGGGCGC426350.029.967067
GAGGGCG433600.029.5974436
GGGCGCG434250.029.4218928
GGCGCGC522250.025.1736269
CTCGACG16300.022.436953
TCTCGAC18450.019.825612
CGCGCCG520350.018.456269
Produced by FastQC (version 0.11.5)