FastQCFastQC Report
Thu 26 May 2016
SRR2048272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2048272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10673276
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTT881070.8254916297489168No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTG188170.17630013502883277No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTC181300.16986349832984737No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTTA163770.15343930017362992No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTCC133860.12541603908678084No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTGG133230.12482577982617521No Hit
ATATCTCGAGGGCGCGCCGGATCCTTTTTTTTTTTTTTTTTTTTTTTTAA126570.11858589621405836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACG388200.093.006191
TCGACGG413300.087.154992
TATCTCG405850.086.377912
CGACGGC436850.082.608873
ATCTCGA429100.081.6312263
GACGGCG441200.081.428954
ATATCTC435200.081.127491
ACGGCGC442650.080.916425
TCTCGAG409600.080.1349644
CGGCGCG449600.079.69556
CTCGAGG423850.076.757955
TCGAGGG425750.076.239276
AGGGCGC439800.073.9462369
CGAGGGC472300.068.778367
GAGGGCG478500.067.9259348
CTCGACG33100.062.023235
TCTCGAC36350.056.9999664
CGCGCCG888350.042.4253129
GCGCGCC895700.041.944638
GGCGCGC890650.041.9164357
Produced by FastQC (version 0.11.5)